BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30329 (634 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 27 3.0 SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 3.9 SPBC12C2.08 |dnm1||dynamin Dnm1|Schizosaccharomyces pombe|chr 2|... 26 3.9 SPAC1F3.09 |mug161||CwfJ family protein|Schizosaccharomyces pomb... 26 5.2 SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 25 6.9 SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 25 6.9 SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr 3... 25 6.9 SPBC405.06 |||DNAJ protein Xdj1 |Schizosaccharomyces pombe|chr 2... 25 9.1 >SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1044 Score = 26.6 bits (56), Expect = 3.0 Identities = 13/48 (27%), Positives = 23/48 (47%) Frame = +3 Query: 84 KSEQKLKMSPDINQIGTQKVRNVSSGEVLELKSFWEQQNVAIVFFRRW 227 K +Q + ++ Q Q + S G LEL F+E+ ++ +RW Sbjct: 101 KIKQSYETPDELQQAAKQFYQLASKGIGLELALFYEEYGSLLIRMQRW 148 >SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1098 Score = 26.2 bits (55), Expect = 3.9 Identities = 20/57 (35%), Positives = 30/57 (52%), Gaps = 5/57 (8%) Frame = -1 Query: 283 ENRRY--FAQLLAQSLQNMQPQR--RKNTIATFCCSQKLFNSKTSPLET-FLTFCVP 128 + R+Y FA+ L+ +L + + R+ A+ CC QKL K L T FLT +P Sbjct: 158 DKRQYLLFARALSSALDIIPNKNSSRRLQFASLCCVQKLICPKQYRLPTEFLTTFLP 214 >SPBC12C2.08 |dnm1||dynamin Dnm1|Schizosaccharomyces pombe|chr 2|||Manual Length = 781 Score = 26.2 bits (55), Expect = 3.9 Identities = 11/33 (33%), Positives = 20/33 (60%) Frame = +3 Query: 78 NHKSEQKLKMSPDINQIGTQKVRNVSSGEVLEL 176 NHK+ LKM+ N++ T + ++S E +E+ Sbjct: 662 NHKNNHPLKMTDLSNEVETMALEDMSEREEVEV 694 >SPAC1F3.09 |mug161||CwfJ family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 561 Score = 25.8 bits (54), Expect = 5.2 Identities = 11/31 (35%), Positives = 17/31 (54%) Frame = -3 Query: 167 NFATGNVSHFLRADLINIRRHFKFLFAFVIY 75 N TG V+HF+ ++ KF +AF +Y Sbjct: 219 NVNTGTVTHFVALAPFKNSKNEKFSYAFTLY 249 >SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1628 Score = 25.4 bits (53), Expect = 6.9 Identities = 20/71 (28%), Positives = 35/71 (49%), Gaps = 2/71 (2%) Frame = +2 Query: 299 KLVGVGVEEAGSKEFSEGKFFDG--DLYYVENISTYQQLGFKRFNILTILTSLLWKQSRD 472 +LV +G G KEF ++ D ++ENI+ F I+ TSLL++Q + Sbjct: 112 RLVSIG---KGLKEFLPEEWLLSRLDCKFLENINVVPNGDFLNRKIVRTNTSLLYRQKKF 168 Query: 473 AISKGKAMGLS 505 + + ++ G S Sbjct: 169 NLLREESEGFS 179 >SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosaccharomyces pombe|chr 1|||Manual Length = 988 Score = 25.4 bits (53), Expect = 6.9 Identities = 13/26 (50%), Positives = 20/26 (76%) Frame = -2 Query: 372 RSPSKNFPSLNSLDPASSTPTPTSLM 295 ++P K+ SLN + P+SSTPTP S++ Sbjct: 606 KTPEKD-RSLN-VTPSSSTPTPASVL 629 >SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 555 Score = 25.4 bits (53), Expect = 6.9 Identities = 13/41 (31%), Positives = 23/41 (56%) Frame = -1 Query: 403 LIS*NVLDVIQISIEKLSFAELLGSGLFDANPDKFDVVLLE 281 L+S + V+ + L +++ G L+D +KFD+ LLE Sbjct: 183 LVSNFLASVLLQTEAPLDISDVCGMNLWDIQNEKFDIRLLE 223 >SPBC405.06 |||DNAJ protein Xdj1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 413 Score = 25.0 bits (52), Expect = 9.1 Identities = 11/27 (40%), Positives = 14/27 (51%) Frame = -2 Query: 483 FEIASRDCFHSSEVKIVKILNLLKPNC 403 +EI+ D F EVK+ N L P C Sbjct: 124 YEISLEDMFKGKEVKLRATRNTLCPRC 150 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,759,420 Number of Sequences: 5004 Number of extensions: 57425 Number of successful extensions: 224 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 214 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 224 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 281707720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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