Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30326
(575 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g05630.1 68415.m00599 autophagy 8d (APG8d) identical to autop... 93 9e-20
At4g21980.1 68417.m03182 autophagy 8a (APG8a) identical to autop... 89 1e-18
At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to autop... 89 2e-18
At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to autop... 89 2e-18
At3g60640.1 68416.m06785 autophagy 8g (APG8g) identical to autop... 86 1e-17
At2g45170.2 68415.m05624 autophagy 8e (APG8e) identical to autop... 86 2e-17
At2g45170.1 68415.m05623 autophagy 8e (APG8e) identical to autop... 86 2e-17
At1g62040.1 68414.m06997 autophagy 8c (APG8c) identical to autop... 84 6e-17
At4g16520.2 68417.m02501 autophagy 8f (APG8f) identical to autop... 84 7e-17
At4g16520.1 68417.m02500 autophagy 8f (APG8f) identical to autop... 84 7e-17
At3g15580.1 68416.m01974 autophagy 8i (APG8i) identical to autop... 74 6e-14
At3g06420.1 68416.m00740 autophagy 8h (APG8h) identical to autop... 69 2e-12
At5g06680.1 68418.m00754 tubulin family protein similar to SP|Q9... 30 0.96
At4g19080.1 68417.m02813 hypothetical protein contains Pfam doma... 28 3.9
At1g49050.1 68414.m05500 aspartyl protease family protein contai... 28 3.9
At5g54830.1 68418.m06829 DOMON domain-containing protein / dopam... 28 5.1
>At2g05630.1 68415.m00599 autophagy 8d (APG8d) identical to
autophagy 8d [Arabidopsis thaliana] GI:19912157;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 120
Score = 93.5 bits (222), Expect = 9e-20
Identities = 38/62 (61%), Positives = 51/62 (82%)
Frame = +2
Query: 80 DLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEI 259
D+D+KKYLVP+DLTVGQF +++RKRI L PE A+F FV N++PPT+A M ++Y+EH DE
Sbjct: 44 DIDRKKYLVPADLTVGQFVYVVRKRIKLSPEKAIFIFVKNILPPTAAIMSAIYEEHKDED 103
Query: 260 SF 265
F
Sbjct: 104 GF 105
Score = 37.1 bits (82), Expect = 0.008
Identities = 15/22 (68%), Positives = 18/22 (81%)
Frame = +3
Query: 3 EGEKIRRKYPDRVPVIVEKAPK 68
E +IR KYPDR+PVIVE+A K
Sbjct: 18 EAARIREKYPDRIPVIVERAEK 39
>At4g21980.1 68417.m03182 autophagy 8a (APG8a) identical to
autophagy 8a [Arabidopsis thaliana] GI:19912151;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 89.4 bits (212), Expect = 1e-18
Identities = 38/62 (61%), Positives = 49/62 (79%)
Frame = +2
Query: 80 DLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEI 259
D+DKKKYLVP+DLTVGQF +++RKRI L E A+F FV N +PPT+A M ++Y+EH DE
Sbjct: 44 DIDKKKYLVPADLTVGQFVYVVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDED 103
Query: 260 SF 265
F
Sbjct: 104 GF 105
Score = 37.1 bits (82), Expect = 0.008
Identities = 15/20 (75%), Positives = 17/20 (85%)
Frame = +3
Query: 3 EGEKIRRKYPDRVPVIVEKA 62
E +IR KYPDR+PVIVEKA
Sbjct: 18 ESSRIREKYPDRIPVIVEKA 37
>At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to
autophagy 8b [Arabidopsis thaliana] GI:19912153;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 89.0 bits (211), Expect = 2e-18
Identities = 37/62 (59%), Positives = 49/62 (79%)
Frame = +2
Query: 80 DLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEI 259
D+DKKKYLVP+DLT+GQF +++RKRI L E A+F FV N +PPT+A M ++Y+EH DE
Sbjct: 44 DIDKKKYLVPADLTIGQFVYVVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDED 103
Query: 260 SF 265
F
Sbjct: 104 GF 105
Score = 35.1 bits (77), Expect = 0.034
Identities = 15/20 (75%), Positives = 17/20 (85%)
Frame = +3
Query: 3 EGEKIRRKYPDRVPVIVEKA 62
E +IR KYP+RVPVIVEKA
Sbjct: 18 ESTRIRAKYPERVPVIVEKA 37
>At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to
autophagy 8b [Arabidopsis thaliana] GI:19912153;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 89.0 bits (211), Expect = 2e-18
Identities = 37/62 (59%), Positives = 49/62 (79%)
Frame = +2
Query: 80 DLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEI 259
D+DKKKYLVP+DLT+GQF +++RKRI L E A+F FV N +PPT+A M ++Y+EH DE
Sbjct: 44 DIDKKKYLVPADLTIGQFVYVVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDED 103
Query: 260 SF 265
F
Sbjct: 104 GF 105
Score = 35.1 bits (77), Expect = 0.034
Identities = 15/20 (75%), Positives = 17/20 (85%)
Frame = +3
Query: 3 EGEKIRRKYPDRVPVIVEKA 62
E +IR KYP+RVPVIVEKA
Sbjct: 18 ESTRIRAKYPERVPVIVEKA 37
>At3g60640.1 68416.m06785 autophagy 8g (APG8g) identical to
autophagy 8g [Arabidopsis thaliana] GI:19912163;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3; supporting cDNA
gi|19912162|dbj|AB073181.1|
Length = 121
Score = 86.2 bits (204), Expect = 1e-17
Identities = 38/70 (54%), Positives = 53/70 (75%)
Frame = +2
Query: 56 ESAEVXLGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSL 235
+S + + ++DKKKYLVP+DLTVGQF ++IRKRI L E A+F FV+NV+PPT A M ++
Sbjct: 36 KSEKSDIPNIDKKKYLVPADLTVGQFVYVIRKRIQLSAEKAIFIFVDNVLPPTGAMMSTI 95
Query: 236 YQEHHDEISF 265
Y E+ +E F
Sbjct: 96 YDENKEEDGF 105
Score = 34.3 bits (75), Expect = 0.059
Identities = 16/31 (51%), Positives = 20/31 (64%)
Frame = +3
Query: 3 EGEKIRRKYPDRVPVIVEKAPKXRLETSTKR 95
E +IR KY DRVPVIVEK+ K + K+
Sbjct: 18 EALRIREKYSDRVPVIVEKSEKSDIPNIDKK 48
>At2g45170.2 68415.m05624 autophagy 8e (APG8e) identical to
autophagy 8e [Arabidopsis thaliana] GI:19912159;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 85.8 bits (203), Expect = 2e-17
Identities = 41/70 (58%), Positives = 52/70 (74%)
Frame = +2
Query: 56 ESAEVXLGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSL 235
E +EV ++DKKKYLVPSDLTVGQF ++IRKRI L E A+F FV+NV+PPT M S+
Sbjct: 39 EKSEVP--NIDKKKYLVPSDLTVGQFVYVIRKRIKLSAEKAIFIFVDNVLPPTGELMSSV 96
Query: 236 YQEHHDEISF 265
Y++ DE F
Sbjct: 97 YEDKKDEDGF 106
Score = 39.1 bits (87), Expect = 0.002
Identities = 17/31 (54%), Positives = 21/31 (67%)
Frame = +3
Query: 3 EGEKIRRKYPDRVPVIVEKAPKXRLETSTKR 95
E +IR KYPDR+PVIVEKA K + K+
Sbjct: 19 EAGRIREKYPDRIPVIVEKAEKSEVPNIDKK 49
>At2g45170.1 68415.m05623 autophagy 8e (APG8e) identical to
autophagy 8e [Arabidopsis thaliana] GI:19912159;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 85.8 bits (203), Expect = 2e-17
Identities = 41/70 (58%), Positives = 52/70 (74%)
Frame = +2
Query: 56 ESAEVXLGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSL 235
E +EV ++DKKKYLVPSDLTVGQF ++IRKRI L E A+F FV+NV+PPT M S+
Sbjct: 39 EKSEVP--NIDKKKYLVPSDLTVGQFVYVIRKRIKLSAEKAIFIFVDNVLPPTGELMSSV 96
Query: 236 YQEHHDEISF 265
Y++ DE F
Sbjct: 97 YEDKKDEDGF 106
Score = 39.1 bits (87), Expect = 0.002
Identities = 17/31 (54%), Positives = 21/31 (67%)
Frame = +3
Query: 3 EGEKIRRKYPDRVPVIVEKAPKXRLETSTKR 95
E +IR KYPDR+PVIVEKA K + K+
Sbjct: 19 EAGRIREKYPDRIPVIVEKAEKSEVPNIDKK 49
>At1g62040.1 68414.m06997 autophagy 8c (APG8c) identical to
autophagy 8c [Arabidopsis thaliana] GI:19912155;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 119
Score = 84.2 bits (199), Expect = 6e-17
Identities = 36/62 (58%), Positives = 48/62 (77%)
Frame = +2
Query: 80 DLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEI 259
++DKKKYLVP+DLTVGQF +++RKRI L E A+F FV N +PPT+A M ++Y E+ DE
Sbjct: 44 NIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFVFVKNTLPPTAAMMSAIYDENKDED 103
Query: 260 SF 265
F
Sbjct: 104 GF 105
Score = 36.7 bits (81), Expect = 0.011
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = +3
Query: 3 EGEKIRRKYPDRVPVIVEKAPKXRLETSTKR 95
E +IR KYPDR+PVIVE+A + + K+
Sbjct: 18 ESSRIREKYPDRIPVIVERAERSDVPNIDKK 48
>At4g16520.2 68417.m02501 autophagy 8f (APG8f) identical to
autophagy 8f [Arabidopsis thaliana] GI:19912161;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 121
Score = 83.8 bits (198), Expect = 7e-17
Identities = 37/61 (60%), Positives = 47/61 (77%)
Frame = +2
Query: 83 LDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEIS 262
+DKKKYLVP+DLTVGQF ++IRKRI L E A+F FV+NV+PP A M S+Y+E D+
Sbjct: 45 IDKKKYLVPADLTVGQFVYVIRKRIKLSAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDG 104
Query: 263 F 265
F
Sbjct: 105 F 105
Score = 41.1 bits (92), Expect = 5e-04
Identities = 18/31 (58%), Positives = 22/31 (70%)
Frame = +3
Query: 3 EGEKIRRKYPDRVPVIVEKAPKXRLETSTKR 95
E +IR KYPDR+PVIVEKA K + T K+
Sbjct: 18 EAARIREKYPDRIPVIVEKAEKSDIPTIDKK 48
>At4g16520.1 68417.m02500 autophagy 8f (APG8f) identical to
autophagy 8f [Arabidopsis thaliana] GI:19912161;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 121
Score = 83.8 bits (198), Expect = 7e-17
Identities = 37/61 (60%), Positives = 47/61 (77%)
Frame = +2
Query: 83 LDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEIS 262
+DKKKYLVP+DLTVGQF ++IRKRI L E A+F FV+NV+PP A M S+Y+E D+
Sbjct: 45 IDKKKYLVPADLTVGQFVYVIRKRIKLSAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDG 104
Query: 263 F 265
F
Sbjct: 105 F 105
Score = 41.1 bits (92), Expect = 5e-04
Identities = 18/31 (58%), Positives = 22/31 (70%)
Frame = +3
Query: 3 EGEKIRRKYPDRVPVIVEKAPKXRLETSTKR 95
E +IR KYPDR+PVIVEKA K + T K+
Sbjct: 18 EAARIREKYPDRIPVIVEKAEKSDIPTIDKK 48
>At3g15580.1 68416.m01974 autophagy 8i (APG8i) identical to
autophagy 8i [Arabidopsis thaliana] GI:19912167;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3; supporting cDNA
gi|21636957|gb|AF492760.1|
Length = 115
Score = 74.1 bits (174), Expect = 6e-14
Identities = 30/61 (49%), Positives = 45/61 (73%)
Frame = +2
Query: 83 LDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEIS 262
++KKK+LVP D++VGQF +++ R+HL P ALF FVNN +P T+A M S+Y+ + D+
Sbjct: 43 IEKKKFLVPRDMSVGQFIYILSARLHLSPGKALFVFVNNTLPQTAALMDSVYESYKDDDG 102
Query: 263 F 265
F
Sbjct: 103 F 103
Score = 28.7 bits (61), Expect = 2.9
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = +3
Query: 3 EGEKIRRKYPDRVPVIVEKAPKXRLETSTKR 95
E +I KYP R+PVI EK K L K+
Sbjct: 16 ESREIIAKYPTRIPVIAEKYCKTDLPAIEKK 46
>At3g06420.1 68416.m00740 autophagy 8h (APG8h) identical to
autophagy 8h [Arabidopsis thaliana] GI:19912165;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3; supporting cDNA
gi|19912164|dbj|AB073182.1|
Length = 119
Score = 69.3 bits (162), Expect = 2e-12
Identities = 31/64 (48%), Positives = 42/64 (65%)
Frame = +2
Query: 74 LGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHD 253
L D++K KYLVP D+TVG F ++ KR+ L P ALF FV+N +P T++ M SLY +
Sbjct: 44 LPDMEKNKYLVPRDMTVGHFIHMLSKRMQLDPSKALFVFVHNTLPQTASRMDSLYNTFKE 103
Query: 254 EISF 265
E F
Sbjct: 104 EDGF 107
Score = 30.7 bits (66), Expect = 0.73
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +3
Query: 3 EGEKIRRKYPDRVPVIVEK 59
E I KYPDR+PVI+EK
Sbjct: 20 ESNNIIAKYPDRIPVIIEK 38
>At5g06680.1 68418.m00754 tubulin family protein similar to
SP|Q96CW5 Gamma-tubulin complex component 3 {Homo
sapiens}
Length = 838
Score = 30.3 bits (65), Expect = 0.96
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = +3
Query: 294 LWKLI-PNYSLSNSFTLSDHMVKFNLSLRLKLCIILW 401
+WK + PN SNSF VK L L+ C +LW
Sbjct: 622 IWKTMKPNCITSNSFVKLQSSVKLQLLSALRRCQVLW 658
>At4g19080.1 68417.m02813 hypothetical protein contains Pfam domain,
PF04578: Protein of unknown function, DUF594
Length = 327
Score = 28.3 bits (60), Expect = 3.9
Identities = 18/63 (28%), Positives = 32/63 (50%)
Frame = +3
Query: 255 RFLFIHSIFRRKCLWKLIPNYSLSNSFTLSDHMVKFNLSLRLKLCIILWYFFYY*LAYNT 434
R L+ +F R CL + NY L F++SD + + + R +L LW F + + + +
Sbjct: 13 RTLYPFRLFIRFCLGIPLINYLLE-FFSISDQVNEIVFTSRTRLTKELWEFIFEEVKHRS 71
Query: 435 IFV 443
+ V
Sbjct: 72 LLV 74
>At1g49050.1 68414.m05500 aspartyl protease family protein contains
Pfam PF00026: Eukaryotic aspartyl protease; contains
similarity to nucellin GI:2290203 from [Hordeum vulgare]
Length = 583
Score = 28.3 bits (60), Expect = 3.9
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +2
Query: 86 DKKKYLVPSDLTVGQFYFLIRKRIHLRPEDAL 181
D KK+ P L +G + +I +++ ++PED L
Sbjct: 485 DVKKFFRPITLQIGSKWLIISRKLLIQPEDYL 516
>At5g54830.1 68418.m06829 DOMON domain-containing protein / dopamine
beta-monooxygenase N-terminal domain-containing protein
contains Pfam PF03351: DOMON domain
Length = 907
Score = 27.9 bits (59), Expect = 5.1
Identities = 17/39 (43%), Positives = 22/39 (56%), Gaps = 5/39 (12%)
Frame = +3
Query: 261 LFIHSIFRRKCLWKL-IPNYSLS----NSFTLSDHMVKF 362
L+ HSIF+ K WK+ + + LS NS L D VKF
Sbjct: 490 LYYHSIFQEKMGWKVQVVDGGLSDMYNNSVNLDDQQVKF 528
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,621,167
Number of Sequences: 28952
Number of extensions: 230684
Number of successful extensions: 586
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 562
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 586
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1121903184
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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