BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30325 (538 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 29 0.44 SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 27 2.3 SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pomb... 26 3.1 SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 25 7.2 SPBC365.05c |slu7||splicing factor Slu7|Schizosaccharomyces pomb... 25 7.2 SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 25 7.2 SPBC2D10.17 |clr1||cryptic loci regulator Clr1|Schizosaccharomyc... 25 9.5 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 25 9.5 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 29.1 bits (62), Expect = 0.44 Identities = 10/22 (45%), Positives = 13/22 (59%) Frame = -1 Query: 88 PEEGEGDQTHHFTGHHFFSDSY 23 PE E + HH GHH F+D + Sbjct: 63 PERDEAEAPHHPKGHHEFNDDF 84 >SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Schizosaccharomyces pombe|chr 2|||Manual Length = 388 Score = 26.6 bits (56), Expect = 2.3 Identities = 25/69 (36%), Positives = 29/69 (42%), Gaps = 3/69 (4%) Frame = +3 Query: 105 QPRMPSGREPLWTA-WSRR*GPAAPKDPTRPPASS--TRSCRYWTPFSKRTLSDL*CCRS 275 +PR RE T W RR GP P +P P T S R+ P R SD Sbjct: 168 EPRRSFAREERSTGDWVRR-GPLPPAEPAESPFGKRRTNSGRFRDP--ARDPSDR---VR 221 Query: 276 DEERRWQRR 302 +E R W RR Sbjct: 222 EEPREWVRR 230 >SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 420 Score = 26.2 bits (55), Expect = 3.1 Identities = 13/46 (28%), Positives = 22/46 (47%) Frame = +1 Query: 295 NDVTGRSTGDFFDGVENYIQSHDVXFKLPIADTQITVSPRNLDNDE 432 N + R +GDF + D+ LP+ D + V P++ D+ E Sbjct: 218 NSLGTRVSGDFDEDFPTGTVFFDLESHLPLLDVALPVLPKSSDSSE 263 >SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 542 Score = 25.0 bits (52), Expect = 7.2 Identities = 18/73 (24%), Positives = 35/73 (47%), Gaps = 4/73 (5%) Frame = +1 Query: 313 STGDFFDGVENYI---QSHDVXFKLPIADTQITVSPRN-LDNDELTLNVKFNKEGARSVG 480 S GDF+D + + Q++++ L + + + N L++ + TLN + N+E S+ Sbjct: 73 SMGDFYDDIRLMVSNAQTYNMPGSLVYECSVLIANTANSLESKDGTLNEEENEEMESSIN 132 Query: 481 EARKAKLKKTSSP 519 E K + P Sbjct: 133 EEHKPGTNEIDVP 145 >SPBC365.05c |slu7||splicing factor Slu7|Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 25.0 bits (52), Expect = 7.2 Identities = 8/20 (40%), Positives = 12/20 (60%) Frame = -1 Query: 367 SRRGTECSSRPRRRSRQWIG 308 S + +C RPR+R +W G Sbjct: 120 SHKVKDCMERPRKRGARWTG 139 >SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 905 Score = 25.0 bits (52), Expect = 7.2 Identities = 11/33 (33%), Positives = 20/33 (60%) Frame = -2 Query: 243 FLKMESSSDMTLYLMQAVGSDPSAQPDLISSTM 145 F K + S + L+ + ++ SDP + P + SST+ Sbjct: 106 FAKSRNESSLKLWDLNSLLSDPKSSPLMQSSTL 138 >SPBC2D10.17 |clr1||cryptic loci regulator Clr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1238 Score = 24.6 bits (51), Expect = 9.5 Identities = 15/45 (33%), Positives = 18/45 (40%) Frame = -2 Query: 183 DPSAQPDLISSTMLSIGVPFQKASLAGCAATLPKRARATKHTTLL 49 DP + L + MLS G + GC A L KH LL Sbjct: 980 DPFEEKVLKAFNMLSKGYAEYRCQWEGCLANLHSLENFIKHVLLL 1024 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 24.6 bits (51), Expect = 9.5 Identities = 10/24 (41%), Positives = 11/24 (45%) Frame = +3 Query: 162 GPAAPKDPTRPPASSTRSCRYWTP 233 G P P PPA S RY+ P Sbjct: 772 GAGPPPPPPPPPAVSAGGSRYYAP 795 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,174,042 Number of Sequences: 5004 Number of extensions: 43528 Number of successful extensions: 124 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 122 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 124 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 222442660 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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