Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30302
(575 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a) ident... 36 0.015
At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1) id... 36 0.015
At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b) ident... 34 0.059
At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly... 34 0.059
At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly... 34 0.059
At2g32410.1 68415.m03960 auxin-resistance protein, putative stro... 34 0.059
At3g18790.1 68416.m02387 expressed protein 32 0.24
At1g05180.2 68414.m00521 auxin-resistance protein AXR1 (AXR1) id... 28 3.9
At5g24340.1 68418.m02868 3'-5' exonuclease domain-containing pro... 27 6.8
At4g30860.1 68417.m04381 SET domain-containing protein low simil... 27 6.8
At1g70550.2 68414.m08120 expressed protein similar to hypothetic... 27 9.0
At1g70550.1 68414.m08119 expressed protein similar to hypothetic... 27 9.0
>At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a)
identical to SUMO activating enzyme 1a [Arabidopsis
thaliana] GI:22652850; contains Pfam profile PF00899:
ThiF family
Length = 322
Score = 36.3 bits (80), Expect = 0.015
Identities = 24/76 (31%), Positives = 34/76 (44%), Gaps = 3/76 (3%)
Frame = +3
Query: 6 FLCPPDK---IGVNRAEGSLERARGLNPMVXVTSHTKGVDELPDSFFTEFDVVCATGLKQ 176
FL PPD+ G AE + + NPMV V+ + L FF +FDVV +
Sbjct: 75 FLIPPDENVYSGKTVAEICSDSLKDFNPMVRVSVEKGDLSMLGTDFFEQFDVVVIGYGSR 134
Query: 177 EQFERINNACRDSNKK 224
+ +N CR K+
Sbjct: 135 ATKKYVNEKCRKLKKR 150
>At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1)
identical to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family; identical to cDNA ubiquitin activating
enzyme E1-related protein (AXR1) GI:304103
Length = 540
Score = 36.3 bits (80), Expect = 0.015
Identities = 23/82 (28%), Positives = 42/82 (51%), Gaps = 7/82 (8%)
Frame = +3
Query: 6 FLCPPDKIGVNRAEGSLERARGLNPMVXVTSHTKGVDELPD-------SFFTEFDVVCAT 164
F+ +G ++A+ + LN V + K ++E PD SFF++F +V AT
Sbjct: 85 FMVDAKSVGQSKAKSVCAFLQELNDSV----NAKFIEENPDTLITTNPSFFSQFTLVIAT 140
Query: 165 GLKQEQFERINNACRDSNKKFI 230
L ++ +++ CRD+N K +
Sbjct: 141 QLVEDSMLKLDRICRDANVKLV 162
>At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b)
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50580;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 34.3 bits (75), Expect = 0.059
Identities = 23/75 (30%), Positives = 34/75 (45%), Gaps = 3/75 (4%)
Frame = +3
Query: 6 FLCPPDK---IGVNRAEGSLERARGLNPMVXVTSHTKGVDELPDSFFTEFDVVCATGLKQ 176
FL PD+ +G AE + + NPMV V+ + L FF +FDVV +
Sbjct: 75 FLILPDENAYVGKTVAEICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKFDVVVIGYSSR 134
Query: 177 EQFERINNACRDSNK 221
+ +N CR+ K
Sbjct: 135 ATKKAVNEKCRNLAK 149
>At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 34.3 bits (75), Expect = 0.059
Identities = 23/75 (30%), Positives = 34/75 (45%), Gaps = 3/75 (4%)
Frame = +3
Query: 6 FLCPPDK---IGVNRAEGSLERARGLNPMVXVTSHTKGVDELPDSFFTEFDVVCATGLKQ 176
FL PD+ +G AE + + NPMV V+ + L FF +FDVV +
Sbjct: 75 FLILPDENAYVGKTVAEICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKFDVVVIGYSSR 134
Query: 177 EQFERINNACRDSNK 221
+ +N CR+ K
Sbjct: 135 ATKKAVNEKCRNLAK 149
>At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 318
Score = 34.3 bits (75), Expect = 0.059
Identities = 23/75 (30%), Positives = 34/75 (45%), Gaps = 3/75 (4%)
Frame = +3
Query: 6 FLCPPDK---IGVNRAEGSLERARGLNPMVXVTSHTKGVDELPDSFFTEFDVVCATGLKQ 176
FL PD+ +G AE + + NPMV V+ + L FF +FDVV +
Sbjct: 75 FLILPDENAYVGKTVAEICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKFDVVVIGYSSR 134
Query: 177 EQFERINNACRDSNK 221
+ +N CR+ K
Sbjct: 135 ATKKAVNEKCRNLAK 149
>At2g32410.1 68415.m03960 auxin-resistance protein, putative strong
similarity to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family
Length = 523
Score = 34.3 bits (75), Expect = 0.059
Identities = 21/84 (25%), Positives = 39/84 (46%), Gaps = 3/84 (3%)
Frame = +3
Query: 6 FLCPPDKIGVNRAEGSLERARGLNPMVXVTSHTKGVDEL---PDSFFTEFDVVCATGLKQ 176
F+ +G +RA+ + LN V + D L SFF++F +V AT L +
Sbjct: 69 FMVDAKSVGQSRAKTVCGFLQELNDSVKANFVEENPDTLISTDPSFFSQFTLVIATQLVE 128
Query: 177 EQFERINNACRDSNKKFICGDVWG 248
+ +++ CR++N + +G
Sbjct: 129 DSMVKLDRICREANVMLVLARSYG 152
>At3g18790.1 68416.m02387 expressed protein
Length = 300
Score = 32.3 bits (70), Expect = 0.24
Identities = 16/38 (42%), Positives = 23/38 (60%)
Frame = +2
Query: 434 PEMRSRLRRGDCGYLL*SSCYAFRDNITGILTRLEEKT 547
PE+R R R D + +S Y +RD+ GIL +LE K+
Sbjct: 147 PELRKRKTRYDIYKRIDASYYGYRDDEDGILEKLERKS 184
>At1g05180.2 68414.m00521 auxin-resistance protein AXR1 (AXR1)
identical to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family; identical to cDNA ubiquitin activating
enzyme E1-related protein (AXR1) GI:304103
Length = 436
Score = 28.3 bits (60), Expect = 3.9
Identities = 13/39 (33%), Positives = 24/39 (61%)
Frame = +3
Query: 114 DELPDSFFTEFDVVCATGLKQEQFERINNACRDSNKKFI 230
++ P+ FFT +V AT L ++ +++ CRD+N K +
Sbjct: 23 NQKPNLFFT---LVIATQLVEDSMLKLDRICRDANVKLV 58
>At5g24340.1 68418.m02868 3'-5' exonuclease domain-containing
protein contains Pfam profile PF01612: 3'-5' exonuclease
Length = 505
Score = 27.5 bits (58), Expect = 6.8
Identities = 17/72 (23%), Positives = 32/72 (44%)
Frame = +3
Query: 30 GVNRAEGSLERARGLNPMVXVTSHTKGVDELPDSFFTEFDVVCATGLKQEQFERINNACR 209
G N A G + R LN M + K V + + + + LK++ R+ ++
Sbjct: 253 GQNIANGVVPRKTTLNTMPMDENLLKIVRKFGERILLKESDLLPKKLKKKTRRRVASSTM 312
Query: 210 DSNKKFICGDVW 245
++NK+ +C W
Sbjct: 313 NTNKQLVCSADW 324
>At4g30860.1 68417.m04381 SET domain-containing protein low
similarity to IL-5 promoter REII-region-binding protein
[Homo sapiens] GI:12642795; contains Pfam profile
PF00856: SET domain
Length = 497
Score = 27.5 bits (58), Expect = 6.8
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +3
Query: 372 SKTSSYICPASECFIS*LEFHQRCV 446
SK+S + CP ECF+ RCV
Sbjct: 157 SKSSKFKCPQHECFVCKQRTQWRCV 181
>At1g70550.2 68414.m08120 expressed protein similar to hypothetical
protein GB:AAD31338 [Arabidopsis thaliana] and to
putative putative carboxyl-terminal peptidase
GB:AAC16072 [Arabidopsis thaliana] contains Pfam profile
PF03080: Arabidopsis proteins of unknown function
Length = 410
Score = 27.1 bits (57), Expect = 9.0
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = -3
Query: 339 SSSGPRLVALCCTISSEYS*STRSEN 262
SSS RL+ L C +SS +S +T S N
Sbjct: 3 SSSFLRLILLLCLVSSSFSSTTSSSN 28
>At1g70550.1 68414.m08119 expressed protein similar to hypothetical
protein GB:AAD31338 [Arabidopsis thaliana] and to
putative putative carboxyl-terminal peptidase
GB:AAC16072 [Arabidopsis thaliana] contains Pfam profile
PF03080: Arabidopsis proteins of unknown function
Length = 465
Score = 27.1 bits (57), Expect = 9.0
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = -3
Query: 339 SSSGPRLVALCCTISSEYS*STRSEN 262
SSS RL+ L C +SS +S +T S N
Sbjct: 58 SSSFLRLILLLCLVSSSFSSTTSSSN 83
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,731,363
Number of Sequences: 28952
Number of extensions: 191320
Number of successful extensions: 509
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 504
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 509
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1121903184
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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