Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30300
(687 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 54 1e-08
SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 50 2e-07
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 50 4e-07
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 48 2e-06
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 45 9e-06
SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 40 3e-04
SPAC1783.04c |hst4||Sir2 family histone deacetylase Hst4|Schizos... 28 1.1
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 27 3.4
SPCC1281.04 |||pyridoxal reductase |Schizosaccharomyces pombe|ch... 26 4.4
SPAC23C11.06c |||hydrolase |Schizosaccharomyces pombe|chr 1|||Ma... 26 4.4
SPAC4A8.16c |tif33|SPAC823.01c|translation initiation factor eIF... 25 7.8
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 54.4 bits (125), Expect = 1e-08
Identities = 31/63 (49%), Positives = 38/63 (60%), Gaps = 1/63 (1%)
Frame = +1
Query: 328 DYIFYTGGTNVGRIVYEAA-TKNLTPVTLELGGKSPVYVDNTVDIVVTAKRILWGKFINV 504
D + +TG T VGR+V AA + NL VTLELGGKSP V N D+ A +G F N
Sbjct: 241 DKVAFTGSTGVGRMVMRAAASSNLKKVTLELGGKSPNIVFNDADLDSAAVWTNYGIFYNS 300
Query: 505 GQL 513
GQ+
Sbjct: 301 GQV 303
Score = 39.1 bits (87), Expect = 6e-04
Identities = 17/42 (40%), Positives = 27/42 (64%)
Frame = +2
Query: 89 NDPYGVVLVIGAWNYPLQLLLLPMAGAIAAGNTVIVKPSELS 214
++P GV I WN+P + +A A+A GNT+I+K +EL+
Sbjct: 158 HEPIGVCGQIIPWNFPFLMCAWKIAPAVACGNTIILKTAELT 199
>SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 50.4 bits (115), Expect = 2e-07
Identities = 28/62 (45%), Positives = 32/62 (51%)
Frame = +1
Query: 328 DYIFYTGGTNVGRIVYEAATKNLTPVTLELGGKSPVYVDNTVDIVVTAKRILWGKFINVG 507
D I +TG T VG IV + A NL VTLE GGKSP V D+ K G N G
Sbjct: 235 DKIAFTGSTKVGVIVQQLAASNLKAVTLECGGKSPFLVFEDADLDQAVKWAALGIMYNSG 294
Query: 508 QL 513
Q+
Sbjct: 295 QI 296
Score = 38.7 bits (86), Expect = 8e-04
Identities = 18/38 (47%), Positives = 24/38 (63%)
Frame = +2
Query: 95 PYGVVLVIGAWNYPLQLLLLPMAGAIAAGNTVIVKPSE 208
P GV I WNYPL + +A A+AAGN +I+K +E
Sbjct: 154 PIGVCGQIVPWNYPLNMAGWKIAPALAAGNCIIIKSAE 191
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 49.6 bits (113), Expect = 4e-07
Identities = 23/49 (46%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
Frame = +2
Query: 95 PYGVVLVIGAWNYPLQLLLLPMAGAIAAGNTVIVKPSELSV-ACSNFVV 238
P GV+ VI WN+PL++ L + AIA+GN V++KPSEL+ +C F +
Sbjct: 162 PRGVIGVITPWNFPLKMALWKLVPAIASGNCVVLKPSELAPWSCLEFAL 210
Score = 49.6 bits (113), Expect = 4e-07
Identities = 21/60 (35%), Positives = 34/60 (56%)
Frame = +1
Query: 331 YIFYTGGTNVGRIVYEAATKNLTPVTLELGGKSPVYVDNTVDIVVTAKRILWGKFINVGQ 510
Y+ +TG G+ + AA +N+ P+TLELGGKSP+ + D+ + + F N G+
Sbjct: 244 YLAFTGSLATGKKIMHAAAENIVPLTLELGGKSPLIICEDADLSLAIPSAAFAIFFNQGE 303
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 47.6 bits (108), Expect = 2e-06
Identities = 23/70 (32%), Positives = 37/70 (52%)
Frame = +2
Query: 5 TLHYLDEWTKPEHPPKGFVNILDEVVIYNDPYGVVLVIGAWNYPLQLLLLPMAGAIAAGN 184
TL ++ +P P + + +P GV+ + +WNYPL L P+ A+ AGN
Sbjct: 97 TLANGEQSLRPTKRPNSLLTSYKGGYVKYEPLGVIAALVSWNYPLHNALGPIISALFAGN 156
Query: 185 TVIVKPSELS 214
++VK SEL+
Sbjct: 157 AIVVKGSELT 166
Score = 39.1 bits (87), Expect = 6e-04
Identities = 19/60 (31%), Positives = 32/60 (53%)
Frame = +1
Query: 331 YIFYTGGTNVGRIVYEAATKNLTPVTLELGGKSPVYVDNTVDIVVTAKRILWGKFINVGQ 510
+I + G + ++V +A K LTP+ LELGGK P + + + ++ G F + GQ
Sbjct: 212 HITFIGSQPIAKLVAASAAKQLTPLCLELGGKDPCILTDDHRLEEILSIVMRGVFQSAGQ 271
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 45.2 bits (102), Expect = 9e-06
Identities = 30/88 (34%), Positives = 46/88 (52%)
Frame = +1
Query: 283 EGGPQETTELLKQRFDYIFYTGGTNVGRIVYEAATKNLTPVTLELGGKSPVYVDNTVDIV 462
E G + TT L ++ + +TG TNVG+I+ + ++ L ++LELGG +P V D+
Sbjct: 229 EHGIELTTNPLIRK---VSFTGSTNVGKILAKQSSSTLKKLSLELGGNAPFIVFEDADLE 285
Query: 463 VTAKRILWGKFINVGQLALLPTTYCARR 546
A ++ KF GQ T CA R
Sbjct: 286 KAADALMACKFRGSGQ-----TCVCANR 308
Score = 41.9 bits (94), Expect = 8e-05
Identities = 16/48 (33%), Positives = 29/48 (60%)
Frame = +2
Query: 62 NILDEVVIYNDPYGVVLVIGAWNYPLQLLLLPMAGAIAAGNTVIVKPS 205
N + ++ P GVV +I WN+P ++ + A+AAG TV+++P+
Sbjct: 148 NPQNRIITIKQPVGVVGIITPWNFPAAMITRKVGAALAAGCTVVIRPA 195
>SPAC139.05 |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 493
Score = 39.9 bits (89), Expect = 3e-04
Identities = 20/64 (31%), Positives = 33/64 (51%)
Frame = +1
Query: 334 IFYTGGTNVGRIVYEAATKNLTPVTLELGGKSPVYVDNTVDIVVTAKRILWGKFINVGQL 513
+ +TG TNVG+I+ + + V++ELGG +P V I + KF + GQ+
Sbjct: 238 VSFTGSTNVGKILMGQSASTIKKVSMELGGNAPFIVFPDFPIDQAVESFCTIKFNSCGQV 297
Query: 514 ALLP 525
+ P
Sbjct: 298 CVCP 301
Score = 31.9 bits (69), Expect = 0.089
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = +2
Query: 77 VVIYNDPYGVVLVIGAWNYPLQLLLLPMAGAIAAGNTVIVKPS 205
++ P GV +I WN+P ++ A+AAG T I P+
Sbjct: 148 LISIKQPVGVSALITPWNFPAAMIARKGGAALAAGCTAIFLPA 190
>SPAC1783.04c |hst4||Sir2 family histone deacetylase
Hst4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 415
Score = 28.3 bits (60), Expect = 1.1
Identities = 12/39 (30%), Positives = 19/39 (48%)
Frame = -1
Query: 546 PSCTVRSREQCKLAHVYKFTPENSFCRHNYVHRVIYVNW 430
P C + + CK+ V + E S C H VI++N+
Sbjct: 266 PDCLIVAGTSCKIPGVKRIIKEMSNCVHKQKGNVIWLNY 304
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 26.6 bits (56), Expect = 3.4
Identities = 11/28 (39%), Positives = 14/28 (50%)
Frame = -3
Query: 205 AGFHDNRVPGSDGSGHRQKQELQGIVPG 122
+G H V G G+ H + EL G PG
Sbjct: 323 SGTHGTHVAGIIGANHPETPELNGAAPG 350
>SPCC1281.04 |||pyridoxal reductase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 333
Score = 26.2 bits (55), Expect = 4.4
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -1
Query: 630 IDRGISVGPLRTIPSGLSWRPPET 559
I G VGP+ GL+WRP +T
Sbjct: 3 IVNGFKVGPIGLGLMGLTWRPKQT 26
>SPAC23C11.06c |||hydrolase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 535
Score = 26.2 bits (55), Expect = 4.4
Identities = 18/44 (40%), Positives = 20/44 (45%), Gaps = 1/44 (2%)
Frame = -1
Query: 396 EIFGGGFVNNSTDVSASSVEDVVKPLFEQLSGLLRTSFE-YDES 268
E FG NN T V E V P E L L TS + Y+ES
Sbjct: 247 ECFGSSSSNNKTTVLVEGGEVSVHPFKEWLLNLQHTSPDLYEES 290
>SPAC4A8.16c |tif33|SPAC823.01c|translation initiation factor
eIF3c|Schizosaccharomyces pombe|chr 1|||Manual
Length = 918
Score = 25.4 bits (53), Expect = 7.8
Identities = 20/53 (37%), Positives = 24/53 (45%), Gaps = 3/53 (5%)
Frame = +1
Query: 34 AGTSSKRI--REHSRRGGYIQ*PVRRGP-RNRRLELSLAAPAFADGRSHRCRE 183
A S KR+ R R YI + GP N R + A+ A ADG RC E
Sbjct: 713 ASDSRKRVISRPFRRMLEYIDRQLFVGPPENTREYIMQASKALADGEWRRCEE 765
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,125,905
Number of Sequences: 5004
Number of extensions: 68521
Number of successful extensions: 201
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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