Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30293
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 155 4e-39
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 155 4e-39
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 155 4e-39
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 69 5e-13
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 45 9e-06
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 43 5e-05
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 32 0.091
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 27 2.6
SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synth... 27 2.6
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.4
SPBP35G2.11c |||transcription related zf-ZZ type zinc finger pro... 26 6.0
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 7.9
SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 25 7.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 155 bits (377), Expect = 4e-39
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +1
Query: 256 ARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANIXTEVKSVEMHHEA 435
ARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA + TEVKSVEMHHE+
Sbjct: 237 ARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHES 296
Query: 436 LQEAVPGDNVGFNVKNVSVKELRR 507
L +PGDNVGFNVKNVSVK++RR
Sbjct: 297 LDAGLPGDNVGFNVKNVSVKDIRR 320
Score = 100 bits (239), Expect = 2e-22
Identities = 43/73 (58%), Positives = 56/73 (76%)
Frame = +2
Query: 2 KMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 181
KMD+T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++G
Sbjct: 154 KMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQG 211
Query: 182 WQVERKEGKADGK 220
WQ E K G GK
Sbjct: 212 WQKETKAGVVKGK 224
Score = 66.1 bits (154), Expect = 5e-12
Identities = 31/60 (51%), Positives = 37/60 (61%)
Frame = +2
Query: 515 CW*LQKQPT*GCCRFYSSSHCA*PSWSISNGYTPVLDCHTXHIACKFAEIKEKVDRRTGK 694
C + P GC F + IS GY+PVLDCHT HIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383
Score = 53.2 bits (122), Expect = 3e-08
Identities = 22/28 (78%), Positives = 24/28 (85%)
Frame = +3
Query: 513 VAGDSKNNPPKGAADFTAQVIVLNHPGQ 596
V GDSKN+PP G A FTAQVI+LNHPGQ
Sbjct: 323 VCGDSKNDPPMGCASFTAQVIILNHPGQ 350
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 155 bits (377), Expect = 4e-39
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +1
Query: 256 ARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANIXTEVKSVEMHHEA 435
ARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA + TEVKSVEMHHE+
Sbjct: 237 ARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHES 296
Query: 436 LQEAVPGDNVGFNVKNVSVKELRR 507
L +PGDNVGFNVKNVSVK++RR
Sbjct: 297 LDAGLPGDNVGFNVKNVSVKDIRR 320
Score = 100 bits (239), Expect = 2e-22
Identities = 43/73 (58%), Positives = 56/73 (76%)
Frame = +2
Query: 2 KMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 181
KMD+T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++G
Sbjct: 154 KMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQG 211
Query: 182 WQVERKEGKADGK 220
WQ E K G GK
Sbjct: 212 WQKETKAGVVKGK 224
Score = 66.1 bits (154), Expect = 5e-12
Identities = 31/60 (51%), Positives = 37/60 (61%)
Frame = +2
Query: 515 CW*LQKQPT*GCCRFYSSSHCA*PSWSISNGYTPVLDCHTXHIACKFAEIKEKVDRRTGK 694
C + P GC F + IS GY+PVLDCHT HIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383
Score = 53.2 bits (122), Expect = 3e-08
Identities = 22/28 (78%), Positives = 24/28 (85%)
Frame = +3
Query: 513 VAGDSKNNPPKGAADFTAQVIVLNHPGQ 596
V GDSKN+PP G A FTAQVI+LNHPGQ
Sbjct: 323 VCGDSKNDPPMGCASFTAQVIILNHPGQ 350
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 155 bits (377), Expect = 4e-39
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +1
Query: 256 ARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANIXTEVKSVEMHHEA 435
ARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA + TEVKSVEMHHE+
Sbjct: 237 ARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHES 296
Query: 436 LQEAVPGDNVGFNVKNVSVKELRR 507
L +PGDNVGFNVKNVSVK++RR
Sbjct: 297 LDAGLPGDNVGFNVKNVSVKDIRR 320
Score = 100 bits (239), Expect = 2e-22
Identities = 43/73 (58%), Positives = 56/73 (76%)
Frame = +2
Query: 2 KMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 181
KMD+T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++G
Sbjct: 154 KMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQG 211
Query: 182 WQVERKEGKADGK 220
WQ E K G GK
Sbjct: 212 WQKETKAGVVKGK 224
Score = 66.1 bits (154), Expect = 5e-12
Identities = 31/60 (51%), Positives = 37/60 (61%)
Frame = +2
Query: 515 CW*LQKQPT*GCCRFYSSSHCA*PSWSISNGYTPVLDCHTXHIACKFAEIKEKVDRRTGK 694
C + P GC F + IS GY+PVLDCHT HIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383
Score = 53.2 bits (122), Expect = 3e-08
Identities = 22/28 (78%), Positives = 24/28 (85%)
Frame = +3
Query: 513 VAGDSKNNPPKGAADFTAQVIVLNHPGQ 596
V GDSKN+PP G A FTAQVI+LNHPGQ
Sbjct: 323 VCGDSKNDPPMGCASFTAQVIILNHPGQ 350
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 69.3 bits (162), Expect = 5e-13
Identities = 36/94 (38%), Positives = 55/94 (58%), Gaps = 2/94 (2%)
Frame = +1
Query: 259 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPANIXTEVKSVEMHHE 432
R TD P + ++DV+ I G GTV GRVE G LK G +V +++ T V +EM +
Sbjct: 247 RKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKK 306
Query: 433 ALQEAVPGDNVGFNVKNVSVKELRRVMLLVTPKT 534
L AV GDN G ++++ ++L+R M++ P T
Sbjct: 307 QLDAAVAGDNCGLLLRSIKREQLKRGMIVAQPGT 340
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 45.2 bits (102), Expect = 9e-06
Identities = 24/55 (43%), Positives = 34/55 (61%), Gaps = 3/55 (5%)
Frame = +2
Query: 26 YSEPRFEEIKKEVSSY-IKKIGYNPAAVAFVPISGWHGDNML--EPSTKMPWFKG 181
+SE RF+EIK VS + IK +G+ + V FVPIS G N++ + S W+KG
Sbjct: 330 WSEDRFQEIKNIVSDFLIKMVGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYKG 384
Score = 40.3 bits (90), Expect = 3e-04
Identities = 27/83 (32%), Positives = 42/83 (50%), Gaps = 1/83 (1%)
Frame = +1
Query: 259 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANIXTEVKSVEMHHEAL 438
+P KPLRL + DVY+ TV GRVE G ++ ++ + VK+V + +
Sbjct: 399 KPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPS 457
Query: 439 QE-AVPGDNVGFNVKNVSVKELR 504
AV GD V + ++ V +LR
Sbjct: 458 STWAVAGDTVTLQLADIEVNQLR 480
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 42.7 bits (96), Expect = 5e-05
Identities = 32/127 (25%), Positives = 57/127 (44%), Gaps = 6/127 (4%)
Frame = +1
Query: 259 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANIXTEVKSV-EMHHEA 435
R + P +P+ YK +GT+ G++E G +K + V+ P N EV ++ + E
Sbjct: 463 RKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEE 520
Query: 436 LQEAVPGDNVGFNVKNVSVKELRRVMLLVTPKTTH-----LRVLQILQLKSLCLTILVNL 600
+ ++ GD V V+ +L T H + + IL+L S+ T +
Sbjct: 521 ISSSICGDQVRLRVRGDDSDVQTGYVLTSTKNPVHATTRFIAQIAILELPSILTTGYSCV 580
Query: 601 KRLHTSV 621
+HT+V
Sbjct: 581 MHIHTAV 587
Score = 25.4 bits (53), Expect = 7.9
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = +2
Query: 596 ISNGYTPVLDCHTXHIACKFAEIKEKVDRRTGKS 697
++ GY+ V+ HT FA++ K+D+ KS
Sbjct: 573 LTTGYSCVMHIHTAVEEVSFAKLLHKLDKTNRKS 606
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 31.9 bits (69), Expect = 0.091
Identities = 17/48 (35%), Positives = 21/48 (43%)
Frame = -2
Query: 270 VSGAGSGRMASRASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPCH 127
V GAG G S+ P+ LPS + P L E +ML CH
Sbjct: 543 VEGAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCH 590
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 27.1 bits (57), Expect = 2.6
Identities = 15/63 (23%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
Frame = +3
Query: 27 TVSPDLRKSRRKYPHTSRRLATTQLL---SLSCPFLDGTETTCWSLQPKCLGSRDGRWSV 197
+ +PD+ ++PHT +R +T + L LSC + +E + P G +D + +
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRCLSCEYSTLSEASDSMSNPLFFGDQDNGYVI 204
Query: 198 RKA 206
++
Sbjct: 205 SQS 207
>SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synthase
Ade3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1323
Score = 27.1 bits (57), Expect = 2.6
Identities = 22/75 (29%), Positives = 34/75 (45%), Gaps = 1/75 (1%)
Frame = -2
Query: 330 GHGTNTTDFVYVLQGKTQGLVSGAGSGRMA-SRASMRHFPSALPSLRSTCHPLNQGILVE 154
G G NT + + + + LVS A S RMA + M +++P+L I +
Sbjct: 701 GKGINTGEALAMGEKPISALVSAAASARMAVAECIMNLVAASIPAL--------DRIRLS 752
Query: 153 GSNMLSPCHPEMGTK 109
+ M +P HP G K
Sbjct: 753 ANWMAAPSHPGEGAK 767
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.4
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 501 QFLDGHVLYVETYIVSRYSFLESFV 427
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPBP35G2.11c |||transcription related zf-ZZ type zinc finger
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 397
Score = 25.8 bits (54), Expect = 6.0
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = +2
Query: 479 RTCPSRNCXVLC 514
RTCPS +C +LC
Sbjct: 5 RTCPSSDCGILC 16
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 7.9
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3
Query: 356 NTPVSTLPTGTVPIPPIL 303
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 347
Score = 25.4 bits (53), Expect = 7.9
Identities = 18/57 (31%), Positives = 28/57 (49%)
Frame = +2
Query: 5 MDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 175
+D +P + F IKK+V Y+ ++ + P + G N LEP TK PW+
Sbjct: 107 LDLKKPLLPQILFGNIKKDV--YLDQV-HRPRHYRGSGSAPLFG-NFLEPLTKTPWY 159
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,155,051
Number of Sequences: 5004
Number of extensions: 68152
Number of successful extensions: 245
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 225
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 243
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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