BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30290 (685 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g29560.1 68417.m04215 expressed protein 32 0.41 At1g63530.1 68414.m07182 hypothetical protein 29 3.8 At5g64440.1 68418.m08095 amidase family protein low similarity t... 28 6.6 At3g30710.1 68416.m03897 hypothetical protein similar to Arginin... 28 6.6 At3g04350.1 68416.m00460 expressed protein 27 8.8 >At4g29560.1 68417.m04215 expressed protein Length = 493 Score = 31.9 bits (69), Expect = 0.41 Identities = 20/58 (34%), Positives = 27/58 (46%) Frame = -3 Query: 446 YDGAEHHDLRADAEEGPERVVHALDPERDVGGRAPGRAALVQAGVGASAFCSTSTVLS 273 ++G E ++ D EE VVH +D VG +A V S F STS VL+ Sbjct: 190 WNGKEITQIKEDMEEDQREVVHNIDHTMTVGLQAHDHEMAVNLRAQISNFESTSEVLT 247 >At1g63530.1 68414.m07182 hypothetical protein Length = 499 Score = 28.7 bits (61), Expect = 3.8 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = +2 Query: 122 EGQNIKITCKLIGKPYSEVTWKYKKDELDNGT 217 EGQNI + K + E+ W+Y K E G+ Sbjct: 279 EGQNISSFKPYVDKSHDELRWEYYKKEAKGGS 310 >At5g64440.1 68418.m08095 amidase family protein low similarity to enantiomerase-selective amidase [Rhodococcus sp.] GI:152052; contains Pfam profile PF01425: Amidase Length = 607 Score = 27.9 bits (59), Expect = 6.6 Identities = 12/25 (48%), Positives = 18/25 (72%) Frame = -3 Query: 374 DPERDVGGRAPGRAALVQAGVGASA 300 DP+R GG + G AA+V AG+ ++A Sbjct: 273 DPKRYTGGSSSGSAAIVAAGLCSAA 297 >At3g30710.1 68416.m03897 hypothetical protein similar to Arginine-tRNA-protein transferase 1 (EC 2.3.2.8)(R-transferase 1) (Arginyltransferase 1) (Arginyl-tRNA--protein transferase 1)(SP:Q9Z2A5) {Mus musculus}; similar to MRP homologs in mycobacteria (SP:P53382), which belong to the MR?/NBP35 family of ATP-binding proteins. Length = 523 Score = 27.9 bits (59), Expect = 6.6 Identities = 21/60 (35%), Positives = 27/60 (45%), Gaps = 2/60 (3%) Frame = +2 Query: 8 EDDYG--NYTCGLKNQTGHIKAWMVTGNVHAKMTKDANVVEGQNIKITCKLIGKPYSEVT 181 ED+ G N C +K Q + V V A D V EG+ I IT KLI Y ++ Sbjct: 420 EDEAGLANLLCHIKGQNYQVPG--VKNMVEAATYADMVVKEGREIAITNKLIALYYQHLS 477 >At3g04350.1 68416.m00460 expressed protein Length = 567 Score = 27.5 bits (58), Expect = 8.8 Identities = 17/43 (39%), Positives = 21/43 (48%) Frame = -2 Query: 201 SSFLYFHVTSEYGLPINLQVILMFCPSTTLASLVILACTFPVT 73 SS LY HV G V+ +FCP A+L I T P+T Sbjct: 345 SSELYVHVKPALGGTFTDIVMWIFCPFNGPATLKIGLFTLPMT 387 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,775,356 Number of Sequences: 28952 Number of extensions: 150164 Number of successful extensions: 500 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 484 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 499 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1447936096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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