BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30282 (538 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 124 6e-30 SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 116 3e-27 SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 75 5e-15 SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 63 2e-11 SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 38 0.001 SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 35 0.007 SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||... 35 0.009 SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 34 0.012 SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1||... 29 0.44 SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||... 29 0.58 SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|ch... 28 0.77 SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 27 1.8 SPAC23H4.07c |srp102||signal recognition particle receptor beta ... 27 1.8 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 25 5.4 SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pom... 25 7.2 SPAPB17E12.12c |||mitochondrial transporter|Schizosaccharomyces ... 25 9.5 SPAC2G11.13 |atg22||autophagy associated protein Atg22 |Schizosa... 25 9.5 SPBP8B7.04 |mug45||sequence orphan|Schizosaccharomyces pombe|chr... 25 9.5 >SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces pombe|chr 2|||Manual Length = 180 Score = 124 bits (300), Expect = 6e-30 Identities = 56/85 (65%), Positives = 67/85 (78%) Frame = +1 Query: 256 GKTTILYKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAI 435 GKTTILYKL++GE+VTTIPTIGFNVE V Y+N+ F VWD+GGQ IRP WR Y+ NT I Sbjct: 29 GKTTILYKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRHYFQNTQGI 88 Query: 436 IYVVDSADRDRIGISKDELVHMLRE 510 I+VVDS DR+RI + +EL ML E Sbjct: 89 IFVVDSNDRERISEAHEELQRMLNE 113 Score = 31.1 bits (67), Expect = 0.11 Identities = 14/30 (46%), Positives = 18/30 (60%) Frame = +2 Query: 197 FRGLLGAREMRILILGLDGAERRQFFINYK 286 F+ L G REMRIL++GLD A + K Sbjct: 9 FQSLFGKREMRILMVGLDAAGKTTILYKLK 38 >SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 116 bits (278), Expect = 3e-27 Identities = 51/89 (57%), Positives = 67/89 (75%) Frame = +1 Query: 256 GKTTILYKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAI 435 GKTTILYKL++ + V TIPT+GFNVE VTYKN+KF VWD+GGQ IRP WR Y+ T + Sbjct: 33 GKTTILYKLKLNQSVVTIPTVGFNVETVTYKNIKFNVWDVGGQDKIRPLWRHYFTGTKGL 92 Query: 436 IYVVDSADRDRIGISKDELVHMLREKN*R 522 I+VVDSAD +RI ++ EL ++ ++ R Sbjct: 93 IFVVDSADSNRISEARQELHRIISDREMR 121 Score = 27.1 bits (57), Expect = 1.8 Identities = 14/35 (40%), Positives = 17/35 (48%) Frame = +2 Query: 182 GLFSYFRGLLGAREMRILILGLDGAERRQFFINYK 286 G F L +EMRIL+LGLD A + K Sbjct: 8 GFSKPFSRLFSNKEMRILMLGLDAAGKTTILYKLK 42 >SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosaccharomyces pombe|chr 1|||Manual Length = 186 Score = 75.4 bits (177), Expect = 5e-15 Identities = 35/90 (38%), Positives = 56/90 (62%) Frame = +1 Query: 244 IGRCGKTTILYKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGN 423 + GKTTIL L +V PT GF + + + L+F +WD+GGQ ++R +W+ Y+ + Sbjct: 24 LDNAGKTTILKCLLNEDVNEVSPTFGFQIRTLEVEGLRFTIWDIGGQKTLRNFWKNYFES 83 Query: 424 TDAIIYVVDSADRDRIGISKDELVHMLREK 513 T+AII+VVDS D R+ ++ L +L E+ Sbjct: 84 TEAIIWVVDSLDDLRLEECRNTLQELLVEE 113 >SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharomyces pombe|chr 2|||Manual Length = 190 Score = 63.3 bits (147), Expect = 2e-11 Identities = 30/87 (34%), Positives = 48/87 (55%) Frame = +1 Query: 244 IGRCGKTTILYKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGN 423 + GKTT+L+ L+ + PT+ E++ N++F +DLGG R WR Y+ Sbjct: 28 LDNAGKTTLLHMLKNDRLAVMQPTLHPTSEELAIGNVRFTTFDLGGHQQARRLWRDYFPE 87 Query: 424 TDAIIYVVDSADRDRIGISKDELVHML 504 + I+Y+VD D +R+ SK EL +L Sbjct: 88 VNGIVYLVDCCDFERLSESKAELDALL 114 >SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 203 Score = 37.9 bits (84), Expect = 0.001 Identities = 21/57 (36%), Positives = 27/57 (47%), Gaps = 4/57 (7%) Frame = +1 Query: 307 IPTIGFNVEQVTY----KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRD 465 I TIG + + T+ K +K Q+WD GQ R YY II V D D+D Sbjct: 38 ISTIGVDFKIRTFELEGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQD 94 >SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 35.1 bits (77), Expect = 0.007 Identities = 16/52 (30%), Positives = 30/52 (57%) Frame = +1 Query: 313 TIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDR 468 T+G + T +L+ +++D+GGQ + R W + N ++II+ V D D+ Sbjct: 179 TLGISEISFTLDHLQIRMFDVGGQRTERRKWIYCFENVNSIIFCVSLNDYDK 230 >SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 34.7 bits (76), Expect = 0.009 Identities = 17/58 (29%), Positives = 27/58 (46%) Frame = +1 Query: 292 EVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRD 465 + + I ++G +Q K +K Q+WD GQ R R YY + V D ++D Sbjct: 45 DFASRIISVGMGNQQ---KRIKLQIWDTAGQEKFRSVARNYYRGAAGAVLVYDVTNKD 99 >SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 34.3 bits (75), Expect = 0.012 Identities = 20/74 (27%), Positives = 33/74 (44%), Gaps = 5/74 (6%) Frame = +1 Query: 256 GKTTILYKLQVGEVVTT-IPTIGFNVE----QVTYKNLKFQVWDLGGQTSIRPYWRCYYG 420 GK+ +L + + I TIG + + ++ K +K Q+WD GQ R YY Sbjct: 21 GKSCLLLRFSEDSFTPSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYR 80 Query: 421 NTDAIIYVVDSADR 462 I+ + D D+ Sbjct: 81 GAMGILLLYDVTDK 94 >SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1|||Manual Length = 214 Score = 29.1 bits (62), Expect = 0.44 Identities = 20/70 (28%), Positives = 29/70 (41%), Gaps = 5/70 (7%) Frame = +1 Query: 256 GKTTILYKLQVGEV-VTTIPTIG--FNVEQVTYKN--LKFQVWDLGGQTSIRPYWRCYYG 420 GK+ +L + E + + TIG F + N +K Q+WD GQ R YY Sbjct: 22 GKSNLLMRFTRNEFNIESKSTIGVEFATRNIVLDNKKIKAQIWDTAGQERYRAITSAYYR 81 Query: 421 NTDAIIYVVD 450 + V D Sbjct: 82 GAVGALIVYD 91 >SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 211 Score = 28.7 bits (61), Expect = 0.58 Identities = 13/54 (24%), Positives = 24/54 (44%) Frame = +1 Query: 349 NLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKDELVHMLRE 510 ++K ++WD GQ + YY N + I V D + +K + + R+ Sbjct: 63 SVKLEIWDTAGQERYKSLAPMYYRNANCAIVVYDITQAASLEKAKSWIKELQRQ 116 >SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 198 Score = 28.3 bits (60), Expect = 0.77 Identities = 19/82 (23%), Positives = 37/82 (45%), Gaps = 5/82 (6%) Frame = +1 Query: 256 GKTTILYKLQVG---EVVTTIPTIGFNVEQVTYKN--LKFQVWDLGGQTSIRPYWRCYYG 420 GKT+++ G E T + F + ++ +N + F +WDLGGQ Sbjct: 22 GKTSLMVTYVQGSFDEESTQTLGVNFMEKTISIRNTEITFSIWDLGGQREFVNMLPMVCN 81 Query: 421 NTDAIIYVVDSADRDRIGISKD 486 + AI+++ D + + + K+ Sbjct: 82 DAVAILFMFDLSRKSTLNSIKE 103 >SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces pombe|chr 2|||Manual Length = 1142 Score = 27.1 bits (57), Expect = 1.8 Identities = 10/31 (32%), Positives = 19/31 (61%) Frame = -2 Query: 483 FRYSYSIPVS*VYNIYYCICVAIVTSPIRPN 391 F ++Y++ S VYN + +C + T+P + N Sbjct: 494 FDFTYNLSESEVYNTVFNLCDELYTTPCKKN 524 >SPAC23H4.07c |srp102||signal recognition particle receptor beta subunit Srp102 |Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 27.1 bits (57), Expect = 1.8 Identities = 24/76 (31%), Positives = 38/76 (50%), Gaps = 5/76 (6%) Frame = +1 Query: 256 GKTTILYKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVW--DLGGQTSIRPYWRCYYG--- 420 GKT++ +L E TT+P+I N E V K+ W DL G + + + Sbjct: 49 GKTSLFCELIYKEKKTTVPSIEPN-EAV----WKYGAWLVDLPGHPRAKRWITTKFSGNY 103 Query: 421 NTDAIIYVVDSADRDR 468 N A+++V++SA DR Sbjct: 104 NVKAVVFVLNSATIDR 119 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 25.4 bits (53), Expect = 5.4 Identities = 13/44 (29%), Positives = 20/44 (45%) Frame = -3 Query: 155 NLLLLIRNVYALRLHIKDF*NCFFVLIYTWSTLNILFNNKYCTF 24 N + +R+ + R HI C ++ WS + L NN Y F Sbjct: 422 NKCIQLRDYFDYRKHI-----CIVTDLFGWSVFDFLKNNNYIPF 460 >SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pombe|chr 2|||Manual Length = 1692 Score = 25.0 bits (52), Expect = 7.2 Identities = 13/40 (32%), Positives = 19/40 (47%) Frame = -2 Query: 474 SYSIPVS*VYNIYYCICVAIVTSPIRPNTGLSPKIPHLEL 355 S S +S + N+ Y C S + PN G + HL+L Sbjct: 697 SISDAISAMQNLKYLSCTNCEMSYVSPNLGKLKHLVHLDL 736 >SPAPB17E12.12c |||mitochondrial transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 317 Score = 24.6 bits (51), Expect = 9.5 Identities = 17/50 (34%), Positives = 24/50 (48%) Frame = +1 Query: 298 VTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVV 447 V IP IG N YKN+K ++ + I ++R Y N II V+ Sbjct: 256 VNRIPGIGHN-----YKNIKSAIFHIYKTEGINGFFRGYSSNMLKIIPVM 300 >SPAC2G11.13 |atg22||autophagy associated protein Atg22 |Schizosaccharomyces pombe|chr 1|||Manual Length = 529 Score = 24.6 bits (51), Expect = 9.5 Identities = 11/34 (32%), Positives = 20/34 (58%) Frame = -3 Query: 359 NLRFLYVTCSTLNPIVGIVVTTSPTCNL*RIVVF 258 N R++ +C + I GI+++ SP+ L +VF Sbjct: 113 NRRYILFSCVIIGSISGIILSWSPSSFLLGKIVF 146 >SPBP8B7.04 |mug45||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 819 Score = 24.6 bits (51), Expect = 9.5 Identities = 11/35 (31%), Positives = 17/35 (48%) Frame = +1 Query: 409 CYYGNTDAIIYVVDSADRDRIGISKDELVHMLREK 513 C YGNT +I V + + IS+ + H + K Sbjct: 246 CTYGNTSSIFQVKNEMSNIKSAISEGYIAHDQQSK 280 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,322,539 Number of Sequences: 5004 Number of extensions: 49730 Number of successful extensions: 122 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 119 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 122 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 222442660 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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