BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30282 (538 letters) Database: mosquito 2352 sequences; 563,979 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DQ182016-1|ABA56308.1| 353|Anopheles gambiae G(alpha)i protein. 40 7e-05 DQ182015-1|ABA56307.1| 353|Anopheles gambiae G(alpha)q2 protein. 36 0.001 EF127647-1|ABL74413.1| 213|Anopheles gambiae Rab5 protein. 31 0.024 DQ182017-1|ABA56309.1| 383|Anopheles gambiae G(alpha)s protein. 29 0.13 AY578812-1|AAT07317.1| 932|Anopheles gambiae wishful thinking p... 24 3.7 DQ974168-1|ABJ52808.1| 447|Anopheles gambiae serpin 9 protein. 23 4.9 AJ438610-4|CAD27476.1| 593|Anopheles gambiae putative transcrip... 23 6.5 >DQ182016-1|ABA56308.1| 353|Anopheles gambiae G(alpha)i protein. Length = 353 Score = 39.5 bits (88), Expect = 7e-05 Identities = 22/69 (31%), Positives = 38/69 (55%), Gaps = 1/69 (1%) Frame = +1 Query: 301 TTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYW-RCYYGNTDAIIYVVDSADRDRIGI 477 T + T G ++K++ F+++D+GGQ S R W C+ G T AII+ V + D + Sbjct: 176 TRVKTTGIVETHFSFKSIHFKMFDVGGQRSERKKWIHCFEGVT-AIIFCVALSGYDLVLA 234 Query: 478 SKDELVHML 504 +E+ M+ Sbjct: 235 EDEEMNRMI 243 >DQ182015-1|ABA56307.1| 353|Anopheles gambiae G(alpha)q2 protein. Length = 353 Score = 35.5 bits (78), Expect = 0.001 Identities = 17/55 (30%), Positives = 31/55 (56%) Frame = +1 Query: 307 IPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRI 471 +PT G + ++F++ D+GGQ S R W + N +II++V ++ D+I Sbjct: 178 VPTTGIIEYPFDLEEIRFRMVDVGGQRSERRKWIHCFENVTSIIFLVALSEYDQI 232 >EF127647-1|ABL74413.1| 213|Anopheles gambiae Rab5 protein. Length = 213 Score = 31.1 bits (67), Expect = 0.024 Identities = 15/53 (28%), Positives = 23/53 (43%) Frame = +1 Query: 352 LKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKDELVHMLRE 510 +KF++WD GQ YY A I V D + D +K + + R+ Sbjct: 73 VKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDIQNSDSFARAKTWVKELQRQ 125 >DQ182017-1|ABA56309.1| 383|Anopheles gambiae G(alpha)s protein. Length = 383 Score = 28.7 bits (61), Expect = 0.13 Identities = 15/53 (28%), Positives = 28/53 (52%) Frame = +1 Query: 352 LKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKDELVHMLRE 510 + F ++D+GGQ R W + + AII+V + + + + +D + LRE Sbjct: 206 VNFHMFDVGGQRDERRKWIQCFNDVTAIIFVTACSSYNMV-LREDPTQNRLRE 257 >AY578812-1|AAT07317.1| 932|Anopheles gambiae wishful thinking protein. Length = 932 Score = 23.8 bits (49), Expect = 3.7 Identities = 15/60 (25%), Positives = 35/60 (58%), Gaps = 2/60 (3%) Frame = +2 Query: 134 SLLIAIN*TKLINKMG-GLF-SYFRGLLGAREMRILILGLDGAERRQFFINYKSVRS*PL 307 S L ++ KL++ +G G + + ++G++ + + + I A+ RQ+F+N + + + PL Sbjct: 235 SNLYNVDNLKLVSMIGQGKYGTVWKGIVNEKPVAVKIFS---AQHRQYFLNERDIYTVPL 291 >DQ974168-1|ABJ52808.1| 447|Anopheles gambiae serpin 9 protein. Length = 447 Score = 23.4 bits (48), Expect = 4.9 Identities = 9/19 (47%), Positives = 12/19 (63%) Frame = -3 Query: 89 FFVLIYTWSTLNILFNNKY 33 F LIY + T ++LFN Y Sbjct: 425 FVFLIYDYGTRSVLFNGVY 443 >AJ438610-4|CAD27476.1| 593|Anopheles gambiae putative transcription factor protein. Length = 593 Score = 23.0 bits (47), Expect = 6.5 Identities = 12/35 (34%), Positives = 17/35 (48%), Gaps = 4/35 (11%) Frame = -2 Query: 450 VYNIYYCICVAI--VTSPIRP--NTGLSPKIPHLE 358 ++ I +C C+ I P TG+SP PH E Sbjct: 408 IHRIQHCTCMLQNNARESISPASGTGMSPSYPHSE 442 Database: mosquito Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 563,979 Number of sequences in database: 2352 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 581,397 Number of Sequences: 2352 Number of extensions: 11785 Number of successful extensions: 18 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 18 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 18 length of database: 563,979 effective HSP length: 60 effective length of database: 422,859 effective search space used: 49897362 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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