BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30280 (372 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 146 7e-37 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 145 2e-36 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 122 2e-29 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 107 4e-25 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 67 7e-13 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 60 1e-10 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 57 8e-10 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 57 8e-10 SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ... 26 1.6 SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|... 26 2.2 SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 26 2.2 SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Ma... 26 2.2 SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces p... 25 2.9 SPBC30D10.14 |||dienelactone hydrolase family|Schizosaccharomyce... 25 3.8 SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p... 25 5.0 SPAC458.03 |||nuclear telomere cap complex subunit |Schizosaccha... 24 6.7 SPBC215.10 |||haloacid dehalogenase-like hydrolase|Schizosacchar... 24 8.8 SPBC146.11c |mug97|meu33|meiotically upregulated gene Mug97|Schi... 24 8.8 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 146 bits (355), Expect = 7e-37 Identities = 70/85 (82%), Positives = 79/85 (92%) Frame = +3 Query: 3 TLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIH 182 TLSSS QASIEIDSLFEGIDFYTSITRARFEEL ADLFR TMEPVE+ LRD+K+DKA ++ Sbjct: 271 TLSSSAQASIEIDSLFEGIDFYTSITRARFEELCADLFRKTMEPVERVLRDSKVDKASVN 330 Query: 183 DIVLVGGSTRIPKVQKLLQDFFNGR 257 +IVLVGGSTRIP+VQKL+ DFFNG+ Sbjct: 331 EIVLVGGSTRIPRVQKLVSDFFNGK 355 Score = 40.3 bits (90), Expect = 9e-05 Identities = 23/52 (44%), Positives = 28/52 (53%) Frame = +2 Query: 215 PQGAEAPARFL*WKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDLLLLD 370 P+ + + F KE KSINPDE +L GD SE+ QDLLLLD Sbjct: 342 PRVQKLVSDFFNGKEPCKSINPDEAVAYGAAVQAAVLTGDTSEKTQDLLLLD 393 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 145 bits (352), Expect = 2e-36 Identities = 68/85 (80%), Positives = 80/85 (94%) Frame = +3 Query: 3 TLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIH 182 TLSSS QASIEIDSL+EGIDFYTSITRARFEEL ADLFR+TMEPVEK LRD+K+DK+ ++ Sbjct: 271 TLSSSAQASIEIDSLYEGIDFYTSITRARFEELCADLFRNTMEPVEKVLRDSKIDKSSVN 330 Query: 183 DIVLVGGSTRIPKVQKLLQDFFNGR 257 +IVLVGGSTRIP++QKL+ DFFNG+ Sbjct: 331 EIVLVGGSTRIPRIQKLVSDFFNGK 355 Score = 39.9 bits (89), Expect = 1e-04 Identities = 22/39 (56%), Positives = 23/39 (58%) Frame = +2 Query: 254 KELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDLLLLD 370 KE KSINPDE IL GD SE+ QDLLLLD Sbjct: 355 KEPCKSINPDEAVAYGAAVQAAILVGDTSEKTQDLLLLD 393 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 122 bits (294), Expect = 2e-29 Identities = 55/85 (64%), Positives = 73/85 (85%) Frame = +3 Query: 6 LSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHD 185 LSS TQ ++E+DSL GIDF +SITRARFE++NA F++T++PV K L+D+K+ KA +HD Sbjct: 276 LSSVTQTTVEVDSLSNGIDFSSSITRARFEDINATTFKATIDPVAKVLKDSKVPKADVHD 335 Query: 186 IVLVGGSTRIPKVQKLLQDFFNGRS 260 IVLVGGSTRIPKVQ+L+ DFF+GR+ Sbjct: 336 IVLVGGSTRIPKVQRLVSDFFDGRA 360 Score = 31.9 bits (69), Expect = 0.033 Identities = 18/40 (45%), Positives = 23/40 (57%), Gaps = 1/40 (2%) Frame = +2 Query: 254 KELNKSINPDEXXXXXXXXXXXIL-HGDKSEEVQDLLLLD 370 + LNKSINPDE +L + S++ QDLLLLD Sbjct: 359 RALNKSINPDEAVAYGAAVQAAVLTNKADSDKTQDLLLLD 398 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 107 bits (258), Expect = 4e-25 Identities = 49/90 (54%), Positives = 69/90 (76%) Frame = +3 Query: 3 TLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIH 182 TLSS IEI+S F G DF +++RA+FEE+N DLF+ T++PVE+ L+D+ + K++I Sbjct: 303 TLSSQKSVRIEIESFFNGQDFSETLSRAKFEEINMDLFKKTLKPVEQVLKDSNLKKSEID 362 Query: 183 DIVLVGGSTRIPKVQKLLQDFFNGRSSTNL 272 DIVLVGGSTRIPKVQ+LL+ FF ++S + Sbjct: 363 DIVLVGGSTRIPKVQELLESFFGKKASKGI 392 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 67.3 bits (157), Expect = 7e-13 Identities = 31/80 (38%), Positives = 53/80 (66%) Frame = +3 Query: 6 LSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHD 185 LS++++A I+ L +GIDF ITR+ E L D+ + +EP+ K+L+ A + ++I+ Sbjct: 308 LSANSEAIASIEELADGIDFRLKITRSVLESLCKDMEDAAVEPINKALKKANLTFSEINS 367 Query: 186 IVLVGGSTRIPKVQKLLQDF 245 I+L GG++RIP +Q L D+ Sbjct: 368 IILFGGASRIPFIQSTLADY 387 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 59.7 bits (138), Expect = 1e-10 Identities = 27/81 (33%), Positives = 48/81 (59%) Frame = +3 Query: 6 LSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHD 185 LS+ T AS +DSL +GIDF++SI R R++ + + V +++ A M+ I + Sbjct: 298 LSNGTTASAAVDSLADGIDFHSSINRLRYDLAASATLNRMADLVTEAVEKANMEPFDISE 357 Query: 186 IVLVGGSTRIPKVQKLLQDFF 248 ++L GG++ PK+ L++ F Sbjct: 358 VILAGGASNTPKLTSLMESIF 378 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 57.2 bits (132), Expect = 8e-10 Identities = 23/58 (39%), Positives = 44/58 (75%) Frame = +3 Query: 75 ITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFF 248 I+RA+FE+L L R T++P +++L+DA + ++I++++LVGG TR+P+V + ++ F Sbjct: 343 ISRAQFEKLVDPLVRRTIDPCKRALKDANLQTSEINEVILVGGMTRMPRVVETVKSIF 400 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 57.2 bits (132), Expect = 8e-10 Identities = 27/81 (33%), Positives = 48/81 (59%) Frame = +3 Query: 6 LSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHD 185 LS++ A + ++ + ID + I R+ FEEL L P+EK+L A + K ++ Sbjct: 280 LSANANAPLNVEMIMNDIDASSFIKRSDFEELIKPLLERLTPPIEKALELAGIKKEDLYS 339 Query: 186 IVLVGGSTRIPKVQKLLQDFF 248 I +VGG TR+P V++++ ++F Sbjct: 340 IEMVGGCTRVPIVKEVIANYF 360 >SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 932 Score = 26.2 bits (55), Expect = 1.6 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = -1 Query: 183 RGFEPYPSWHHGETSPLAP 127 + +P P+ HG+TSPL+P Sbjct: 420 KAIKPMPTSIHGQTSPLSP 438 >SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|chr 3|||Manual Length = 828 Score = 25.8 bits (54), Expect = 2.2 Identities = 14/46 (30%), Positives = 22/46 (47%) Frame = -3 Query: 322 DSSLDSCTISYGLVRVNRFVELLPLKKSCRSFCTLGIRVEPPTSTI 185 DS + C S+ + + L L ++ CTL I + PTST+ Sbjct: 472 DSDVMDCCFSFLASILTHSIALPRLLRTKIDLCTLQISLSNPTSTV 517 >SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 538 Score = 25.8 bits (54), Expect = 2.2 Identities = 17/53 (32%), Positives = 27/53 (50%) Frame = +3 Query: 42 SLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVG 200 SL G+D+YT I E +A +S+ E +K D + D++ D + VG Sbjct: 331 SLARGLDYYTGIIYEAVTEASAPKIKSSAE--KKKSADPEADRSN-DDSIGVG 380 >SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Manual Length = 396 Score = 25.8 bits (54), Expect = 2.2 Identities = 10/39 (25%), Positives = 21/39 (53%) Frame = -3 Query: 301 TISYGLVRVNRFVELLPLKKSCRSFCTLGIRVEPPTSTI 185 T+S G+ R+ F + +PL + + + +PP ++I Sbjct: 2 TVSEGIARIQAFRKQVPLVEKNENITFFNLSFQPPMNSI 40 >SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 591 Score = 25.4 bits (53), Expect = 2.9 Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 4/34 (11%) Frame = +1 Query: 109 ICSGLPWSQ----WRSLSVMPRWIRLKSTILYWW 198 ICS LP + W + S PR+ R S ++ WW Sbjct: 133 ICSALPAAGSIYLWAAESAGPRFGRFVSFLVAWW 166 >SPBC30D10.14 |||dienelactone hydrolase family|Schizosaccharomyces pombe|chr 2|||Manual Length = 249 Score = 25.0 bits (52), Expect = 3.8 Identities = 11/21 (52%), Positives = 14/21 (66%) Frame = +3 Query: 210 RIPKVQKLLQDFFNGRSSTNL 272 + P+ QKL DFF+ R S NL Sbjct: 91 KTPEDQKLCNDFFSTRISPNL 111 >SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 404 Score = 24.6 bits (51), Expect = 5.0 Identities = 9/18 (50%), Positives = 14/18 (77%) Frame = -3 Query: 283 VRVNRFVELLPLKKSCRS 230 VR+ RF+EL+ KK C++ Sbjct: 195 VRLQRFIELIKSKKLCQA 212 >SPAC458.03 |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 868 Score = 24.2 bits (50), Expect = 6.7 Identities = 9/14 (64%), Positives = 12/14 (85%) Frame = -2 Query: 257 PSIKEILQELLHLG 216 P +KEIL+EL H+G Sbjct: 15 PRLKEILKELQHIG 28 Score = 23.8 bits (49), Expect = 8.8 Identities = 14/39 (35%), Positives = 21/39 (53%) Frame = -3 Query: 166 SILASRRDFSTGSMVDLNRSAFSSSKRARVIDV*KSIPS 50 S +R D TGS+ L R FSS+ R+I+ +P+ Sbjct: 258 SYSTARDDRITGSLATLLRLNFSSTHFLRLIEFYWGVPT 296 >SPBC215.10 |||haloacid dehalogenase-like hydrolase|Schizosaccharomyces pombe|chr 2|||Manual Length = 302 Score = 23.8 bits (49), Expect = 8.8 Identities = 10/18 (55%), Positives = 12/18 (66%) Frame = +1 Query: 1 GPCHRPHKRALR*ILSLR 54 GP H+PH R LR + LR Sbjct: 34 GPDHKPHPRNLRALKYLR 51 >SPBC146.11c |mug97|meu33|meiotically upregulated gene Mug97|Schizosaccharomyces pombe|chr 2|||Manual Length = 335 Score = 23.8 bits (49), Expect = 8.8 Identities = 8/22 (36%), Positives = 14/22 (63%) Frame = +3 Query: 207 TRIPKVQKLLQDFFNGRSSTNL 272 T +PK QK+ + +F G + N+ Sbjct: 50 THVPKRQKVSEPYFRGETERNV 71 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,556,887 Number of Sequences: 5004 Number of extensions: 30588 Number of successful extensions: 102 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 91 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 102 length of database: 2,362,478 effective HSP length: 65 effective length of database: 2,037,218 effective search space used: 118158644 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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