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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30280
         (372 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)                      138   1e-33
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)             136   7e-33
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)                       132   1e-31
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)                      117   4e-27
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)                 77   5e-15
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)                 67   4e-12
SB_38725| Best HMM Match : No HMM Matches (HMM E-Value=.)              67   4e-12
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)              66   1e-11
SB_47390| Best HMM Match : No HMM Matches (HMM E-Value=.)              37   0.005
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)              33   0.056
SB_30598| Best HMM Match : No HMM Matches (HMM E-Value=.)              32   0.17 
SB_56121| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   0.52 
SB_24074| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   0.69 
SB_22339| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   0.91 
SB_25455| Best HMM Match : DUF948 (HMM E-Value=0.11)                   28   2.1  
SB_8228| Best HMM Match : No HMM Matches (HMM E-Value=.)               28   2.8  
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)                 27   3.7  
SB_48536| Best HMM Match : EPSP_synthase (HMM E-Value=8.2e-06)         27   4.9  
SB_8091| Best HMM Match : C1_4 (HMM E-Value=1.3)                       27   4.9  
SB_57167| Best HMM Match : Ribosomal_MRP8 (HMM E-Value=3)              27   6.4  
SB_26282| Best HMM Match : Tctex-1 (HMM E-Value=3.1e-14)               27   6.4  
SB_53255| Best HMM Match : No HMM Matches (HMM E-Value=.)              26   8.5  
SB_8303| Best HMM Match : START (HMM E-Value=0.0093)                   26   8.5  

>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
          Length = 1097

 Score =  138 bits (334), Expect = 1e-33
 Identities = 67/87 (77%), Positives = 77/87 (88%), Gaps = 2/87 (2%)
 Frame = +3

Query: 3   TLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKM--DKAQ 176
           TLSSSTQASIEIDSLFEGIDFYTSITRARFEELN DLF+ T EPVE+++RD+K+   K  
Sbjct: 273 TLSSSTQASIEIDSLFEGIDFYTSITRARFEELNQDLFKKTTEPVEQAIRDSKIPGGKES 332

Query: 177 IHDIVLVGGSTRIPKVQKLLQDFFNGR 257
           IHDIVLVGGSTRIPK+QK+LQ+ F G+
Sbjct: 333 IHDIVLVGGSTRIPKIQKMLQELFGGK 359



 Score = 49.6 bits (113), Expect = 8e-07
 Identities = 24/39 (61%), Positives = 26/39 (66%)
 Frame = +2

Query: 254 KELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDLLLLD 370
           KELNKSINPDE           ILHGDK + V+DLLLLD
Sbjct: 359 KELNKSINPDEAVAYGAAVQAAILHGDKDDTVKDLLLLD 397


>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 746

 Score =  136 bits (328), Expect = 7e-33
 Identities = 63/86 (73%), Positives = 80/86 (93%), Gaps = 1/86 (1%)
 Frame = +3

Query: 3   TLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMD-KAQI 179
           TLSSSTQASIEIDSL++GIDFYTSITRARFEE+NA LF+ T+EPVEK+++D+K++ K +I
Sbjct: 366 TLSSSTQASIEIDSLYDGIDFYTSITRARFEEMNAHLFKKTLEPVEKAIKDSKLESKEKI 425

Query: 180 HDIVLVGGSTRIPKVQKLLQDFFNGR 257
            +IV+VGGSTRIPK+Q LLQ+FFNG+
Sbjct: 426 DEIVMVGGSTRIPKIQSLLQNFFNGK 451



 Score = 52.4 bits (120), Expect = 1e-07
 Identities = 26/39 (66%), Positives = 26/39 (66%)
 Frame = +2

Query: 254 KELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDLLLLD 370
           KELNKSINPDE           ILHGDKSE  QDLLLLD
Sbjct: 451 KELNKSINPDEAVAYGAAVQAAILHGDKSEATQDLLLLD 489


>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
          Length = 640

 Score =  132 bits (318), Expect = 1e-31
 Identities = 58/86 (67%), Positives = 77/86 (89%)
 Frame = +3

Query: 3   TLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIH 182
           TLSSST+ASIE+DSLFEGIDFYT I+RARFE+L +DLF S ++PV K+L DAK  K+ IH
Sbjct: 274 TLSSSTEASIEVDSLFEGIDFYTKISRARFEDLCSDLFLSCLDPVNKALSDAKFSKSDIH 333

Query: 183 DIVLVGGSTRIPKVQKLLQDFFNGRS 260
           ++VLVGGSTR+PK+QK+L+++F+G+S
Sbjct: 334 EVVLVGGSTRVPKIQKMLEEYFSGKS 359



 Score = 45.6 bits (103), Expect = 1e-05
 Identities = 22/39 (56%), Positives = 26/39 (66%)
 Frame = +2

Query: 254 KELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDLLLLD 370
           K LNKSINPDE           IL GDKS+EV+D+LL+D
Sbjct: 358 KSLNKSINPDEAVAYGAAVQAAILSGDKSDEVKDVLLVD 396


>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
          Length = 1327

 Score =  117 bits (281), Expect = 4e-27
 Identities = 54/84 (64%), Positives = 70/84 (83%)
 Frame = +3

Query: 6    LSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHD 185
            LS+  QA +EI+S FEG DF   +TRARFEELNA LF+ST++PV+K L DA + K++IH+
Sbjct: 971  LSTQHQARVEIESFFEGEDFSEMLTRARFEELNAKLFKSTLKPVQKVLEDADLKKSEIHE 1030

Query: 186  IVLVGGSTRIPKVQKLLQDFFNGR 257
            IVLVGGSTRIPKVQ+L++DFF G+
Sbjct: 1031 IVLVGGSTRIPKVQQLVKDFFEGK 1054


>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
          Length = 437

 Score = 77.0 bits (181), Expect = 5e-15
 Identities = 32/88 (36%), Positives = 60/88 (68%), Gaps = 3/88 (3%)
 Frame = +3

Query: 3   TLSSSTQASIEIDSLFEG---IDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKA 173
           T ++     + + SL EG   + F  +I+R  FE +N DLF+  ++P+ + L + ++ K 
Sbjct: 303 TRTNEVTIKLMLPSLSEGKKIVKFKETISRKMFETINEDLFKKVLQPIRRVLAEVELPKE 362

Query: 174 QIHDIVLVGGSTRIPKVQKLLQDFFNGR 257
           ++ ++VLVGGSTR+PK+++L+Q+FF+G+
Sbjct: 363 EVDEVVLVGGSTRVPKIRQLIQEFFDGK 390


>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
          Length = 212

 Score = 67.3 bits (157), Expect = 4e-12
 Identities = 29/58 (50%), Positives = 45/58 (77%)
 Frame = +3

Query: 75  ITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFF 248
           ++R++FE L ADL   T+ P +K L+DA+++K +I D++LVGG TR+PKVQ+ +QD F
Sbjct: 53  LSRSKFESLVADLINRTVGPCKKCLQDAEINKGEIGDVLLVGGMTRMPKVQQTVQDIF 110


>SB_38725| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 766

 Score = 67.3 bits (157), Expect = 4e-12
 Identities = 29/58 (50%), Positives = 45/58 (77%)
 Frame = +3

Query: 75  ITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFF 248
           ++R++FE L ADL   T+ P +K L+DA+++K +I D++LVGG TR+PKVQ+ +QD F
Sbjct: 108 LSRSKFESLVADLINRTVGPCKKCLQDAEINKGEIGDVLLVGGMTRMPKVQQTVQDIF 165


>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1019

 Score = 65.7 bits (153), Expect = 1e-11
 Identities = 31/77 (40%), Positives = 45/77 (58%)
 Frame = +3

Query: 6   LSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHD 185
           LS++ +   +I+ +F+G DF   +TR   EE+  DLF     PV ++L+ A M    I  
Sbjct: 258 LSANNEIFAQIEGVFDGKDFRVKVTREELEEMCQDLFDRVAGPVNRALKSASMTMNDIDS 317

Query: 186 IVLVGGSTRIPKVQKLL 236
           +VLVGG  R+PKVQ  L
Sbjct: 318 VVLVGGGIRVPKVQDAL 334


>SB_47390| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 168

 Score = 37.1 bits (82), Expect = 0.005
 Identities = 13/32 (40%), Positives = 26/32 (81%)
 Frame = +3

Query: 162 MDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGR 257
           + ++ +  ++LVGG+TR PK+Q+LL+++F G+
Sbjct: 35  LKQSSMIQVILVGGATRTPKIQQLLKNYFVGK 66


>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1068

 Score = 33.5 bits (73), Expect = 0.056
 Identities = 18/61 (29%), Positives = 34/61 (55%)
 Frame = +3

Query: 9   SSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDI 188
           +++++  I I+   E  D +  + RA+FEEL ADL +    P+  +L  A+  K  + ++
Sbjct: 336 ANTSEIPINIECFMEDKDVHGRMKRAQFEELAADLLKLVEAPLRSAL--AQSGKYSVKNV 393

Query: 189 V 191
           V
Sbjct: 394 V 394


>SB_30598| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 809

 Score = 31.9 bits (69), Expect = 0.17
 Identities = 25/92 (27%), Positives = 45/92 (48%), Gaps = 5/92 (5%)
 Frame = +3

Query: 81  RARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIP-----KVQKLLQDF 245
           +  +E+L+ADL    + P+  +L+D   D   I    L+  +  I      KVQ+LL   
Sbjct: 390 QTEWEDLSADLLPLVLNPIISALQDDDDDVRAIAASALLPIAESIVQLAPNKVQELLMTL 449

Query: 246 FNGRSSTNLLTLTRP*LMVQLSRLLSCTVTSL 341
           +N     + LT +   +M+ L+ +L+C   S+
Sbjct: 450 WNTLVELDDLTASTNSIMLLLAGILACPSVSM 481


>SB_56121| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 553

 Score = 30.3 bits (65), Expect = 0.52
 Identities = 26/69 (37%), Positives = 36/69 (52%), Gaps = 7/69 (10%)
 Frame = +1

Query: 94  RS*TPICSGLPW--SQWRSLSVMPRWIRL--KSTILY--WWVAPLVSPRCRSSCKI-SLM 252
           RS  P+ S  P    ++R L ++PRW  +  KST+L+    V P  S R RS   + SL 
Sbjct: 221 RSSPPVASLDPGVRQEYRPLHMLPRWTLVYGKSTVLFTSCLVGPWCSARVRSPPPVASLD 280

Query: 253 EGAQQIY*P 279
            G +Q Y P
Sbjct: 281 PGVRQEYGP 289



 Score = 29.5 bits (63), Expect = 0.91
 Identities = 26/69 (37%), Positives = 36/69 (52%), Gaps = 7/69 (10%)
 Frame = +1

Query: 94  RS*TPICSGLPW--SQWRSLSVMPRWIRL--KSTILY--WWVAPLVSPRCRSSCKI-SLM 252
           RS  P+ S  P    ++  L ++PRW  +  KST+L+    V P  S R RSS  + SL 
Sbjct: 371 RSSPPVASLDPGVRQEYGPLHLLPRWTLVYGKSTVLFTSCLVGPWCSARVRSSPPVASLD 430

Query: 253 EGAQQIY*P 279
            G +Q Y P
Sbjct: 431 PGVRQEYGP 439


>SB_24074| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 87

 Score = 29.9 bits (64), Expect = 0.69
 Identities = 11/33 (33%), Positives = 21/33 (63%)
 Frame = +3

Query: 174 QIHDIVLVGGSTRIPKVQKLLQDFFNGRSSTNL 272
           +I  + +VGGSTRIP ++ ++++ F     T +
Sbjct: 6   EIDSVEIVGGSTRIPAIKDIIKNVFGKELMTTM 38


>SB_22339| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 285

 Score = 29.5 bits (63), Expect = 0.91
 Identities = 26/69 (37%), Positives = 36/69 (52%), Gaps = 7/69 (10%)
 Frame = +1

Query: 94  RS*TPICSGLPW--SQWRSLSVMPRWIRL--KSTILY--WWVAPLVSPRCRSSCKI-SLM 252
           RS  P+ S  P    ++  L ++PRW  +  KST+L+    V P  S R RSS  + SL 
Sbjct: 103 RSSPPVASLDPGVRQEYGPLHLLPRWTLVYGKSTVLFTSCLVGPWCSARVRSSPPVASLD 162

Query: 253 EGAQQIY*P 279
            G +Q Y P
Sbjct: 163 PGVRQEYGP 171


>SB_25455| Best HMM Match : DUF948 (HMM E-Value=0.11)
          Length = 260

 Score = 28.3 bits (60), Expect = 2.1
 Identities = 15/42 (35%), Positives = 22/42 (52%), Gaps = 2/42 (4%)
 Frame = +3

Query: 36  IDSLFEGIDFYTSITRARFEELNAD--LFRSTMEPVEKSLRD 155
           +DSL   +DF T+ T A F +LN      R T+  +  SL +
Sbjct: 168 LDSLAVRVDFTTNTTNALFTQLNTTDAAIRDTLSQINSSLSE 209


>SB_8228| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 280

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 13/58 (22%), Positives = 31/58 (53%), Gaps = 1/58 (1%)
 Frame = +3

Query: 69  TSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGG-STRIPKVQKLLQ 239
           T  ++ARF++   DL   T++P+ ++++    D   + +++  G    R+   + L+Q
Sbjct: 176 TEHSKARFKQQLTDLVIHTLQPIRENIQSLSKDPGLVSNVLETGAEKARLLAEETLVQ 233


>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
          Length = 592

 Score = 27.5 bits (58), Expect = 3.7
 Identities = 12/26 (46%), Positives = 17/26 (65%)
 Frame = +3

Query: 6   LSSSTQASIEIDSLFEGIDFYTSITR 83
           LS+   A+  +DSL EGIDF   ++R
Sbjct: 240 LSTLESANCSVDSLCEGIDFQGQVSR 265


>SB_48536| Best HMM Match : EPSP_synthase (HMM E-Value=8.2e-06)
          Length = 231

 Score = 27.1 bits (57), Expect = 4.9
 Identities = 12/36 (33%), Positives = 23/36 (63%)
 Frame = -2

Query: 257 PSIKEILQELLHLGDTSGATHQYNIVDLSLIHLGIT 150
           P +KE+ ++++   D S A++ Y+I+ LS I   +T
Sbjct: 130 PFVKEVAKKIVVESDWSSASYFYSIIALSNIGSSVT 165


>SB_8091| Best HMM Match : C1_4 (HMM E-Value=1.3)
          Length = 308

 Score = 27.1 bits (57), Expect = 4.9
 Identities = 11/22 (50%), Positives = 12/22 (54%)
 Frame = +2

Query: 182 RYCTGGWLHSYPQGAEAPARFL 247
           RYC  G LH  PQ  + PA  L
Sbjct: 204 RYCIKGPLHRLPQAVQEPATIL 225


>SB_57167| Best HMM Match : Ribosomal_MRP8 (HMM E-Value=3)
          Length = 698

 Score = 26.6 bits (56), Expect = 6.4
 Identities = 14/46 (30%), Positives = 22/46 (47%)
 Frame = +1

Query: 136 WRSLSVMPRWIRLKSTILYWWVAPLVSPRCRSSCKISLMEGAQQIY 273
           W  + V P W+ +  T LY      +SP+     K+S    +QQ+Y
Sbjct: 237 WLDVFVTPDWLDVFYTSLYNQRLGYLSPKFEKLVKLSGTHLSQQVY 282


>SB_26282| Best HMM Match : Tctex-1 (HMM E-Value=3.1e-14)
          Length = 273

 Score = 26.6 bits (56), Expect = 6.4
 Identities = 16/42 (38%), Positives = 23/42 (54%)
 Frame = -3

Query: 370 IKQQQILHLLRLVTVQDSSLDSCTISYGLVRVNRFVELLPLK 245
           I+QQQ    LRLVT Q +  ++  +   L ++  FV LL  K
Sbjct: 16  IEQQQDSAYLRLVTPQRTETETYVVLCNLRQLQTFVSLLIAK 57


>SB_53255| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 247

 Score = 26.2 bits (55), Expect = 8.5
 Identities = 10/15 (66%), Positives = 12/15 (80%)
 Frame = +3

Query: 216 PKVQKLLQDFFNGRS 260
           PK  +LLQDFF+G S
Sbjct: 229 PKASRLLQDFFSGMS 243


>SB_8303| Best HMM Match : START (HMM E-Value=0.0093)
          Length = 522

 Score = 26.2 bits (55), Expect = 8.5
 Identities = 14/30 (46%), Positives = 17/30 (56%)
 Frame = -1

Query: 261 SSFH*RNLAGASAPWGYEWSHPPVQYRGFE 172
           SS H R +    AP   +WSHP V + GFE
Sbjct: 188 SSGHARCVTRQQAPRVCQWSHPKV-FAGFE 216


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,193,046
Number of Sequences: 59808
Number of extensions: 249905
Number of successful extensions: 3025
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 2970
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3023
length of database: 16,821,457
effective HSP length: 74
effective length of database: 12,395,665
effective search space used: 607387585
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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