Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30277
(424 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 35 0.006
SPAC26H5.11 |||spore wall assembly protein |Schizosaccharomyces ... 27 1.2
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 27 1.6
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 2.1
SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 26 2.1
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 26 2.1
SPAC2F3.03c |rpa49|rpa51|DNA-directed RNA polymerase I complex |... 26 2.8
SPAP8A3.14c |||mitochondrial inner membrane protein |Schizosacch... 25 3.7
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 25 3.7
SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase Snf21|... 25 3.7
SPBC577.15c |||NASP family histone binding protein|Schizosacchar... 25 4.8
SPCC18B5.06 |erf1|sup45|translation release factor eRF1|Schizosa... 25 6.4
SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomy... 25 6.4
SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 24 8.5
SPCC736.12c |||conserved protein|Schizosaccharomyces pombe|chr 3... 24 8.5
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 34.7 bits (76), Expect = 0.006
Identities = 18/48 (37%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
Frame = -3
Query: 386 PLGPRAPRGPLAPVSPLGPSMPGGPCGPLGPRSHR-QYPPSNGAVRQV 246
P P P+ P+APV+P PS+P P P+ P + PP+ V +V
Sbjct: 599 PEAPSVPQPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEV 646
Score = 33.9 bits (74), Expect = 0.010
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = -3
Query: 386 PLGPRAPRGPLAPVSPLGPSMPGGPCGPLGP 294
P P P+ P+APV P PS+P P P+ P
Sbjct: 584 PEVPSVPQPPVAPVVPEAPSVPQPPVAPVAP 614
Score = 33.1 bits (72), Expect = 0.018
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = -3
Query: 386 PLGPRAPRGPLAPVSPLGPSMPGGPCGPLGPRS 288
P P AP+ P APV P PS+P P P+ P +
Sbjct: 539 PEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEA 571
Score = 32.7 bits (71), Expect = 0.024
Identities = 18/48 (37%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = -3
Query: 386 PLGPRAPRGPLAPVSPLGPSMPGGPCGPLGPRSHR-QYPPSNGAVRQV 246
P P P+ P APV P PS+P P P+ P + PP+ V +V
Sbjct: 629 PEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEV 676
Score = 31.9 bits (69), Expect = 0.042
Identities = 15/39 (38%), Positives = 20/39 (51%)
Frame = -3
Query: 386 PLGPRAPRGPLAPVSPLGPSMPGGPCGPLGPRSHRQYPP 270
P P P+ P APV P PS+P P P+ P + + P
Sbjct: 659 PEAPSVPQPPAAPVVPEVPSVPQPPAVPVVPEAGQLNEP 697
Score = 30.7 bits (66), Expect = 0.098
Identities = 15/36 (41%), Positives = 22/36 (61%), Gaps = 3/36 (8%)
Frame = -3
Query: 386 PLGPRA---PRGPLAPVSPLGPSMPGGPCGPLGPRS 288
P+ P A P+ P+APV+P PS+P P P+ P +
Sbjct: 566 PVVPEALSVPQPPVAPVAPEVPSVPQPPVAPVVPEA 601
Score = 29.9 bits (64), Expect = 0.17
Identities = 16/42 (38%), Positives = 21/42 (50%), Gaps = 2/42 (4%)
Frame = -3
Query: 413 SIRSIKSRDP--LGPRAPRGPLAPVSPLGPSMPGGPCGPLGP 294
S S K+ P + P P+ P APV P PS+ P P+ P
Sbjct: 498 SSASQKAAQPSVITPSVPQPPAAPVVPEAPSVHQPPAAPVAP 539
>SPAC26H5.11 |||spore wall assembly protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 965
Score = 27.1 bits (57), Expect = 1.2
Identities = 14/32 (43%), Positives = 21/32 (65%)
Frame = -2
Query: 363 RSSRSSITFGSLHARRSLWSPRSSLASTIPAF 268
+SS S + S+ +R L+SPR SL ++PAF
Sbjct: 385 QSSLLSFEWISIISRALLFSPRPSLLISVPAF 416
>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
Length = 596
Score = 26.6 bits (56), Expect = 1.6
Identities = 20/42 (47%), Positives = 25/42 (59%)
Frame = -2
Query: 369 APRSSRSSITFGSLHARRSLWSPRSSLASTIPAFQRSRSSST 244
A RSS SS +F S + R ++ RSS P F RSRSSS+
Sbjct: 169 ATRSSLSSSSFDS-YLRPNVSRSRSS--GNAPPFLRSRSSSS 207
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.2 bits (55), Expect = 2.1
Identities = 15/41 (36%), Positives = 17/41 (41%), Gaps = 4/41 (9%)
Frame = -3
Query: 377 PRAPRGPLAPVSPLGPSM----PGGPCGPLGPRSHRQYPPS 267
P AP +APV P P M P P P Q PP+
Sbjct: 1448 PPAPMHAVAPVQPKAPGMVTNAPAPSSAPAPPAPVSQLPPA 1488
>SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 681
Score = 26.2 bits (55), Expect = 2.1
Identities = 21/57 (36%), Positives = 31/57 (54%)
Frame = -2
Query: 360 SSRSSITFGSLHARRSLWSPRSSLASTIPAFQRSRSSSTLYA*LPSPPSSFSIGCSN 190
+S S TFGS S + P + L++T P+F S SSST + PP++ G +N
Sbjct: 90 TSMSGYTFGS-----SNFVPYNLLSNT-PSFTTSHSSSTTFV----PPATMGGGLNN 136
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 26.2 bits (55), Expect = 2.1
Identities = 25/66 (37%), Positives = 34/66 (51%), Gaps = 2/66 (3%)
Frame = -2
Query: 378 TTCAPRSSRSSI--TFGSLHARRSLWSPRSSLASTIPAFQRSRSSSTLYA*LPSPPSSFS 205
TT + S SSI TF S+ + S S SS +ST + S +SST + S PSS S
Sbjct: 558 TTISSGISSSSIPSTFSSVSSILS--SSTSSPSSTSLSISSSSTSSTFSSASTSSPSSIS 615
Query: 204 IGCSNN 187
S++
Sbjct: 616 SSISSS 621
>SPAC2F3.03c |rpa49|rpa51|DNA-directed RNA polymerase I complex
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 425
Score = 25.8 bits (54), Expect = 2.8
Identities = 24/85 (28%), Positives = 31/85 (36%)
Frame = +2
Query: 17 SDGKKCVPRIPLAIAEPLPLVRASSRCYAPCDTVTWLTRKVRQLNDQXXXXXXXXXXXXE 196
S +K V +PL+ + PLV S Y P +T L +K Q N E
Sbjct: 18 SGDEKSVKILPLSESSLPPLVTTISGFYPPENTRFQLLKKSNQSNRDASLIGRTERVQFE 77
Query: 197 HPMLKDDGGEGNYAYRVLDERLRWK 271
E NY V D+ R K
Sbjct: 78 AKNQSTATVEANYCLAVADKTGRVK 102
>SPAP8A3.14c |||mitochondrial inner membrane protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 677
Score = 25.4 bits (53), Expect = 3.7
Identities = 7/14 (50%), Positives = 10/14 (71%)
Frame = -1
Query: 73 WKRFCNSQRYPWDT 32
WKR+C + Y W+T
Sbjct: 336 WKRWCRVKEYSWNT 349
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 25.4 bits (53), Expect = 3.7
Identities = 14/39 (35%), Positives = 17/39 (43%)
Frame = -3
Query: 386 PLGPRAPRGPLAPVSPLGPSMPGGPCGPLGPRSHRQYPP 270
P PR+ P+ P G S P P P + RQ PP
Sbjct: 340 PPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPP 378
>SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase
Snf21|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1199
Score = 25.4 bits (53), Expect = 3.7
Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 2/56 (3%)
Frame = +1
Query: 25 QEMCPTDTAGYCRTSSTCARLLSMLRAV*--HSDVVNQESETAERSTARNTRRLKE 186
Q +C RT + AR RAV HS + +E AER+ + + LKE
Sbjct: 273 QTVCAHGREINVRTKNAQARAQKANRAVLAYHSHIEKEEQRRAERNAKQRLQALKE 328
>SPBC577.15c |||NASP family histone binding
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 396
Score = 25.0 bits (52), Expect = 4.8
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = +2
Query: 200 PMLKDDGGEGNYAYRVLDERLRWKAGIVDASEDRGDHKDRRA 325
P ++D + N A+ VLD ++ VDA D D K R A
Sbjct: 158 PASEEDEDDFNVAWEVLDLTRVMQSKAVDAYPDSKDEKIRLA 199
>SPCC18B5.06 |erf1|sup45|translation release factor
eRF1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 390
Score = 24.6 bits (51), Expect = 6.4
Identities = 16/56 (28%), Positives = 27/56 (48%)
Frame = +2
Query: 203 MLKDDGGEGNYAYRVLDERLRWKAGIVDASEDRGDHKDRRAWRDPKVILEREDLGA 370
+LKD E A + +R +++ D + DR+AW P +L+ +LGA
Sbjct: 256 ILKDPAVESKLADTKYVQEIR----VLNKFYDVMNEDDRKAWYGPNHVLKAFELGA 307
>SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 385
Score = 24.6 bits (51), Expect = 6.4
Identities = 19/45 (42%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
Frame = -2
Query: 375 TCAPRSSRSSITFGSLHARRSLWSPRSS-LASTIPAFQRSRSSST 244
T A RSS S+ GS +++S SS STI S SSST
Sbjct: 15 TSAARSSSVSVETGSCVRYTTIYSSGSSEFTSTITPETPSSSSST 59
>SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 793
Score = 24.2 bits (50), Expect = 8.5
Identities = 10/29 (34%), Positives = 15/29 (51%)
Frame = +1
Query: 22 WQEMCPTDTAGYCRTSSTCARLLSMLRAV 108
WQE+ T YC + T +RL L ++
Sbjct: 494 WQELRSTKDKDYCLSLETNSRLKQQLSSI 522
>SPCC736.12c |||conserved protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 437
Score = 24.2 bits (50), Expect = 8.5
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -2
Query: 363 RSSRSSITFGSLHARRSLWSPRS 295
RSS+S +L A RSLW P S
Sbjct: 10 RSSKSIPELPNLEALRSLWPPPS 32
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,668,375
Number of Sequences: 5004
Number of extensions: 30724
Number of successful extensions: 116
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 103
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 150383836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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