BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30272 (499 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 149 2e-37 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 146 1e-36 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 81 1e-16 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 49 4e-07 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.13 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 0.90 SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 27 2.1 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 2.7 SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces pombe... 26 2.7 SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual 26 2.7 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 2.7 SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 3.6 SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 26 3.6 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 4.8 SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces... 25 6.3 SPAC31G5.14 |gcv1|n313|glycine decarboxylase T subunit|Schizosac... 25 8.4 SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Sc... 25 8.4 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 149 bits (362), Expect = 2e-37 Identities = 67/82 (81%), Positives = 74/82 (90%) Frame = +1 Query: 4 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEIT 183 Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE SV EIT Sbjct: 233 QVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEIT 292 Query: 184 NACFEPANQMVKCDPRHGKYMA 249 N CFEP NQMVKCDPR G+YMA Sbjct: 293 NQCFEPYNQMVKCDPRAGRYMA 314 Score = 138 bits (334), Expect = 4e-34 Identities = 59/80 (73%), Positives = 70/80 (87%) Frame = +3 Query: 255 MLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVC 434 +LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++AKV RAVC Sbjct: 317 LLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVC 376 Query: 435 MLSNTTAIAEAWARLDHKFD 494 MLSNTT+IAEAW+RLDHKFD Sbjct: 377 MLSNTTSIAEAWSRLDHKFD 396 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 146 bits (355), Expect = 1e-36 Identities = 66/82 (80%), Positives = 73/82 (89%) Frame = +1 Query: 4 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEIT 183 Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE SV EIT Sbjct: 237 QVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEIT 296 Query: 184 NACFEPANQMVKCDPRHGKYMA 249 N CFEP NQMVKCDPR G+YMA Sbjct: 297 NQCFEPYNQMVKCDPRTGRYMA 318 Score = 142 bits (345), Expect = 2e-35 Identities = 60/80 (75%), Positives = 72/80 (90%) Frame = +3 Query: 255 MLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVC 434 +LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +AKV RAVC Sbjct: 321 LLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVC 380 Query: 435 MLSNTTAIAEAWARLDHKFD 494 MLSNTT+IAEAW+RLDHKFD Sbjct: 381 MLSNTTSIAEAWSRLDHKFD 400 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 80.6 bits (190), Expect = 1e-16 Identities = 33/80 (41%), Positives = 52/80 (65%) Frame = +1 Query: 7 IVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITN 186 +++ +T S RF G LN DL + N+VP+PR+HF +V +AP+ + + + +SV E+T Sbjct: 232 VMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQ 291 Query: 187 ACFEPANQMVKCDPRHGKYM 246 F+ N MV DPRHG+Y+ Sbjct: 292 QMFDANNMMVAADPRHGRYL 311 Score = 39.9 bits (89), Expect = 2e-04 Identities = 24/80 (30%), Positives = 41/80 (51%) Frame = +3 Query: 258 LYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCM 437 L+RG V K+V+ I +++TK + FV+W P + PP DL + + Sbjct: 316 LFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK-----DL---KMSATF 367 Query: 438 LSNTTAIAEAWARLDHKFDS 497 + N+T+I E + RL +F + Sbjct: 368 IGNSTSIQEIFRRLGDQFSA 387 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 48.8 bits (111), Expect = 4e-07 Identities = 25/77 (32%), Positives = 40/77 (51%), Gaps = 3/77 (3%) Frame = +1 Query: 7 IVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS---AEKAYHEQLSVAE 177 ++S+ T +LR+ G +N DL +L+P PR HF L +Y P + E + +V + Sbjct: 235 VMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLD 294 Query: 178 ITNACFEPANQMVKCDP 228 + P NQMV +P Sbjct: 295 VMRRLLLPKNQMVSVNP 311 Score = 37.9 bits (84), Expect = 8e-04 Identities = 20/82 (24%), Positives = 43/82 (52%) Frame = +3 Query: 249 LLMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRA 428 +L + +G+ P DV+ ++ I+ +R F+ W P +V ++ + P + ++ + Sbjct: 320 ILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGL--- 376 Query: 429 VCMLSNTTAIAEAWARLDHKFD 494 ML+N T+IA + R ++D Sbjct: 377 --MLANHTSIASLFKRTLDQYD 396 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 30.7 bits (66), Expect = 0.13 Identities = 16/45 (35%), Positives = 21/45 (46%) Frame = +1 Query: 265 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 399 VV P + RP++P P LS V PV+ V + PP P Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596 Score = 25.0 bits (52), Expect = 6.3 Identities = 14/45 (31%), Positives = 18/45 (40%) Frame = +1 Query: 265 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 399 VV P + RP++P P S PV V + PP P Sbjct: 642 VVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686 Score = 24.6 bits (51), Expect = 8.4 Identities = 15/45 (33%), Positives = 19/45 (42%) Frame = +1 Query: 268 VTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 402 V S P+ P +P PSV + V P + SV P PE Sbjct: 541 VPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPE 585 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.9 bits (59), Expect = 0.90 Identities = 17/49 (34%), Positives = 26/49 (53%) Frame = +2 Query: 65 PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 211 P+++ P S + T +SS+P P N+ PSP S + AS+ P R Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279 >SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 793 Score = 26.6 bits (56), Expect = 2.1 Identities = 18/64 (28%), Positives = 24/64 (37%) Frame = +3 Query: 234 WQVHGLLMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLA 413 W+ Y + KDVN + TI +W PTG ++ Y V D Sbjct: 141 WRHSSFAQYYLYNTETKDVNM-LGQDNEHWTISLAEWSPTGHQLSFVYNNDLYVRKND-G 198 Query: 414 KVQR 425 VQR Sbjct: 199 NVQR 202 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 2.7 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +2 Query: 128 QSSLPRRPTMNSFPSPRSQTHASSPPTR 211 + SLPRRP+ +P S T ++ PP + Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765 >SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 526 Score = 26.2 bits (55), Expect = 2.7 Identities = 10/22 (45%), Positives = 13/22 (59%) Frame = -2 Query: 297 PRSHPWVRRHHGTASASHVLAM 232 P + WV RH G+ SA H L + Sbjct: 468 PWVYEWVSRHRGSISAEHGLGL 489 >SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual Length = 1339 Score = 26.2 bits (55), Expect = 2.7 Identities = 14/47 (29%), Positives = 27/47 (57%) Frame = +3 Query: 240 VHGLLMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQ 380 V G + L R ++ + N AIA+I +I+F ++ P GF ++++ Sbjct: 294 VRGAVRLMRFLMLQEVKNDAIASITLSSSIEFTEF-PLGFNENVDFE 339 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 26.2 bits (55), Expect = 2.7 Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%) Frame = +1 Query: 301 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 387 +LP KPS+ +++S V+P S STTS+P Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36 >SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 751 Score = 25.8 bits (54), Expect = 3.6 Identities = 16/47 (34%), Positives = 26/47 (55%) Frame = +2 Query: 62 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 202 SPS LT L S++ S + + + R+ T +S SP+ Q+ S+P Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664 >SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 25.8 bits (54), Expect = 3.6 Identities = 16/51 (31%), Positives = 31/51 (60%) Frame = +2 Query: 89 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 241 LTP + S +S + +S +R ++N+ PS + +H+SS T + PV+++ Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSN 178 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.4 bits (53), Expect = 4.8 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%) Frame = +1 Query: 298 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 393 P PS+P+++SN ST G+Q V V + W Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564 >SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces pombe|chr 2|||Manual Length = 476 Score = 25.0 bits (52), Expect = 6.3 Identities = 15/37 (40%), Positives = 16/37 (43%) Frame = +1 Query: 280 PRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPP 390 PR LP K VL T + VSR TTS P Sbjct: 293 PRPLLNSKLPRKTLVLDLDETLIHSVSRGSRTTSGQP 329 >SPAC31G5.14 |gcv1|n313|glycine decarboxylase T subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 387 Score = 24.6 bits (51), Expect = 8.4 Identities = 8/18 (44%), Positives = 10/18 (55%) Frame = -2 Query: 291 SHPWVRRHHGTASASHVL 238 SH W R H G SH++ Sbjct: 58 SHKWTREHSGLFDVSHMV 75 >SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Schizosaccharomyces pombe|chr 2|||Manual Length = 651 Score = 24.6 bits (51), Expect = 8.4 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = +1 Query: 46 ALNVDLTEFQTNLVPYPRIHFPLVT 120 A +V++ E +TN +P HFP T Sbjct: 368 AQSVEVPENETNQIPTTEEHFPATT 392 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,180,994 Number of Sequences: 5004 Number of extensions: 46793 Number of successful extensions: 194 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 177 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 194 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 196153982 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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