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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30272
         (499 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   149   2e-37
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   146   1e-36
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    81   1e-16
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    49   4e-07
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    31   0.13 
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    28   0.90 
SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po...    27   2.1  
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||...    26   2.7  
SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces pombe...    26   2.7  
SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual    26   2.7  
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch...    26   2.7  
SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   3.6  
SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c...    26   3.6  
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po...    25   4.8  
SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces...    25   6.3  
SPAC31G5.14 |gcv1|n313|glycine decarboxylase T subunit|Schizosac...    25   8.4  
SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Sc...    25   8.4  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  149 bits (362), Expect = 2e-37
 Identities = 67/82 (81%), Positives = 74/82 (90%)
 Frame = +1

Query: 4   QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEIT 183
           Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE  SV EIT
Sbjct: 233 QVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEIT 292

Query: 184 NACFEPANQMVKCDPRHGKYMA 249
           N CFEP NQMVKCDPR G+YMA
Sbjct: 293 NQCFEPYNQMVKCDPRAGRYMA 314



 Score =  138 bits (334), Expect = 4e-34
 Identities = 59/80 (73%), Positives = 70/80 (87%)
 Frame = +3

Query: 255 MLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVC 434
           +LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI  +PP  + G ++AKV RAVC
Sbjct: 317 LLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVC 376

Query: 435 MLSNTTAIAEAWARLDHKFD 494
           MLSNTT+IAEAW+RLDHKFD
Sbjct: 377 MLSNTTSIAEAWSRLDHKFD 396


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  146 bits (355), Expect = 1e-36
 Identities = 66/82 (80%), Positives = 73/82 (89%)
 Frame = +1

Query: 4   QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEIT 183
           Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE  SV EIT
Sbjct: 237 QVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEIT 296

Query: 184 NACFEPANQMVKCDPRHGKYMA 249
           N CFEP NQMVKCDPR G+YMA
Sbjct: 297 NQCFEPYNQMVKCDPRTGRYMA 318



 Score =  142 bits (345), Expect = 2e-35
 Identities = 60/80 (75%), Positives = 72/80 (90%)
 Frame = +3

Query: 255 MLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVC 434
           +LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP  VPG  +AKV RAVC
Sbjct: 321 LLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVC 380

Query: 435 MLSNTTAIAEAWARLDHKFD 494
           MLSNTT+IAEAW+RLDHKFD
Sbjct: 381 MLSNTTSIAEAWSRLDHKFD 400


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 80.6 bits (190), Expect = 1e-16
 Identities = 33/80 (41%), Positives = 52/80 (65%)
 Frame = +1

Query: 7   IVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITN 186
           +++ +T S RF G LN DL +   N+VP+PR+HF +V +AP+ +   +  + +SV E+T 
Sbjct: 232 VMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQ 291

Query: 187 ACFEPANQMVKCDPRHGKYM 246
             F+  N MV  DPRHG+Y+
Sbjct: 292 QMFDANNMMVAADPRHGRYL 311



 Score = 39.9 bits (89), Expect = 2e-04
 Identities = 24/80 (30%), Positives = 41/80 (51%)
 Frame = +3

Query: 258 LYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCM 437
           L+RG V  K+V+  I +++TK +  FV+W P      +   PP      DL   + +   
Sbjct: 316 LFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK-----DL---KMSATF 367

Query: 438 LSNTTAIAEAWARLDHKFDS 497
           + N+T+I E + RL  +F +
Sbjct: 368 IGNSTSIQEIFRRLGDQFSA 387


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 48.8 bits (111), Expect = 4e-07
 Identities = 25/77 (32%), Positives = 40/77 (51%), Gaps = 3/77 (3%)
 Frame = +1

Query: 7   IVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS---AEKAYHEQLSVAE 177
           ++S+ T +LR+ G +N DL     +L+P PR HF L +Y P  +    E     + +V +
Sbjct: 235 VMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLD 294

Query: 178 ITNACFEPANQMVKCDP 228
           +      P NQMV  +P
Sbjct: 295 VMRRLLLPKNQMVSVNP 311



 Score = 37.9 bits (84), Expect = 8e-04
 Identities = 20/82 (24%), Positives = 43/82 (52%)
 Frame = +3

Query: 249 LLMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRA 428
           +L + +G+  P DV+ ++  I+ +R   F+ W P   +V ++ + P +     ++ +   
Sbjct: 320 ILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGL--- 376

Query: 429 VCMLSNTTAIAEAWARLDHKFD 494
             ML+N T+IA  + R   ++D
Sbjct: 377 --MLANHTSIASLFKRTLDQYD 396


>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 30.7 bits (66), Expect = 0.13
 Identities = 16/45 (35%), Positives = 21/45 (46%)
 Frame = +1

Query: 265 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 399
           VV   P +  RP++P  P  LS     V PV+  V +   PP  P
Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596



 Score = 25.0 bits (52), Expect = 6.3
 Identities = 14/45 (31%), Positives = 18/45 (40%)
 Frame = +1

Query: 265 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 399
           VV   P +  RP++P  P   S       PV   V +   PP  P
Sbjct: 642 VVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686



 Score = 24.6 bits (51), Expect = 8.4
 Identities = 15/45 (33%), Positives = 19/45 (42%)
 Frame = +1

Query: 268 VTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 402
           V S P+    P +P  PSV    +  V P + SV      P  PE
Sbjct: 541 VPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPE 585


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 27.9 bits (59), Expect = 0.90
 Identities = 17/49 (34%), Positives = 26/49 (53%)
 Frame = +2

Query: 65   PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 211
            P+++      P S +    T +SS+P  P  N+ PSP S + AS+ P R
Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279


>SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 793

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 18/64 (28%), Positives = 24/64 (37%)
 Frame = +3

Query: 234 WQVHGLLMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLA 413
           W+       Y  +   KDVN  +       TI   +W PTG ++   Y     V   D  
Sbjct: 141 WRHSSFAQYYLYNTETKDVNM-LGQDNEHWTISLAEWSPTGHQLSFVYNNDLYVRKND-G 198

Query: 414 KVQR 425
            VQR
Sbjct: 199 NVQR 202


>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1158

 Score = 26.2 bits (55), Expect = 2.7
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +2

Query: 128 QSSLPRRPTMNSFPSPRSQTHASSPPTR 211
           + SLPRRP+     +P S T ++ PP +
Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765


>SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 526

 Score = 26.2 bits (55), Expect = 2.7
 Identities = 10/22 (45%), Positives = 13/22 (59%)
 Frame = -2

Query: 297 PRSHPWVRRHHGTASASHVLAM 232
           P  + WV RH G+ SA H L +
Sbjct: 468 PWVYEWVSRHRGSISAEHGLGL 489


>SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1339

 Score = 26.2 bits (55), Expect = 2.7
 Identities = 14/47 (29%), Positives = 27/47 (57%)
 Frame = +3

Query: 240 VHGLLMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQ 380
           V G + L R  ++ +  N AIA+I    +I+F ++ P GF   ++++
Sbjct: 294 VRGAVRLMRFLMLQEVKNDAIASITLSSSIEFTEF-PLGFNENVDFE 339


>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 628

 Score = 26.2 bits (55), Expect = 2.7
 Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
 Frame = +1

Query: 301 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 387
           +LP KPS+   +++S   V+P S   STTS+P
Sbjct: 5   TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36


>SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 751

 Score = 25.8 bits (54), Expect = 3.6
 Identities = 16/47 (34%), Positives = 26/47 (55%)
 Frame = +2

Query: 62  SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 202
           SPS  LT  L   S++  S  + + + R+ T +S  SP+ Q+  S+P
Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664


>SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 474

 Score = 25.8 bits (54), Expect = 3.6
 Identities = 16/51 (31%), Positives = 31/51 (60%)
 Frame = +2

Query: 89  LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 241
           LTP + S +S +  +S  +R ++N+ PS  + +H+SS  T   +  PV+++
Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSN 178


>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 665

 Score = 25.4 bits (53), Expect = 4.8
 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
 Frame = +1

Query: 298 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 393
           P  PS+P+++SN ST  G+Q V   V   +   W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564


>SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 476

 Score = 25.0 bits (52), Expect = 6.3
 Identities = 15/37 (40%), Positives = 16/37 (43%)
 Frame = +1

Query: 280 PRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPP 390
           PR      LP K  VL    T +  VSR   TTS  P
Sbjct: 293 PRPLLNSKLPRKTLVLDLDETLIHSVSRGSRTTSGQP 329


>SPAC31G5.14 |gcv1|n313|glycine decarboxylase T
           subunit|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 387

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 8/18 (44%), Positives = 10/18 (55%)
 Frame = -2

Query: 291 SHPWVRRHHGTASASHVL 238
           SH W R H G    SH++
Sbjct: 58  SHKWTREHSGLFDVSHMV 75


>SPBC428.07 |meu6||meiotic chromosome segregation protein
           Meu6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 651

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = +1

Query: 46  ALNVDLTEFQTNLVPYPRIHFPLVT 120
           A +V++ E +TN +P    HFP  T
Sbjct: 368 AQSVEVPENETNQIPTTEEHFPATT 392


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,180,994
Number of Sequences: 5004
Number of extensions: 46793
Number of successful extensions: 194
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 194
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 196153982
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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