BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30268 (643 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9 |Schizos... 31 0.11 SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|... 31 0.11 SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 31 0.14 SPAC644.06c |cdr1|nim1|GIN4 family protein kinase Cdr1|Schizosac... 30 0.25 SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy... 29 0.57 SPCC18B5.11c |cds1||replication checkpoint kinase Cds1|Schizosac... 29 0.75 SPCC1919.01 |ppk34|SPCC830.12|serine/threonine protein kinase Pp... 29 0.75 SPAC30C2.08 |||conserved fungal protein|Schizosaccharomyces pomb... 28 1.3 SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccha... 28 1.3 SPBC11B10.09 |cdc2|swo2|cyclin-dependent protein kinase Cdc2|Sch... 27 1.7 SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 27 1.7 SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 27 3.0 SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos... 26 5.3 SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schiz... 26 5.3 SPAC1006.05c |och1||alpha-1,6-mannosyltransferase Och1 |Schizosa... 26 5.3 SPBC409.07c |wis1|spc2, smf2|MAP kinase kinase Wis1|Schizosaccha... 25 7.0 SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|c... 25 7.0 SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyce... 25 9.3 SPAC1D4.13 |byr1|ste1, ste3|MAP kinase kinase Byr1|Schizosacchar... 25 9.3 >SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9 |Schizosaccharomyces pombe|chr 1|||Manual Length = 532 Score = 31.5 bits (68), Expect = 0.11 Identities = 16/56 (28%), Positives = 31/56 (55%) Frame = +3 Query: 288 ELPFAKXNDSSVLDRLKTGALEWSVPASMPQSVADILKRCWSQSPTDRPQFAEVCE 455 E PF S+VL R+ G +++P+ + S +D++++ PT R + AE+ + Sbjct: 217 EFPFEDVTISNVLSRVCKGI--YTIPSFVSSSASDLIRQMLMVLPTSRIKVAEIMQ 270 Score = 26.6 bits (56), Expect = 3.0 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = +1 Query: 49 HRLTHRDIAARNCLITSKLQLKLS 120 HR+ HRD+ N L+ L +KL+ Sbjct: 139 HRVAHRDLKLENILLNKDLVVKLT 162 >SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|Schizosaccharomyces pombe|chr 3|||Manual Length = 288 Score = 31.5 bits (68), Expect = 0.11 Identities = 23/69 (33%), Positives = 36/69 (52%), Gaps = 2/69 (2%) Frame = +1 Query: 49 HRLTHRDIAARNCLITSKLQLKLSCPALTRGPLSREYYKLHDQVIPLRWCPLEVVLEGD- 225 +R+ HRD+ +N LI S+ +LKL+ L R + ++V+ L W VL G Sbjct: 120 NRVLHRDLKPQNLLINSRGELKLADFGLARS-IGIPVNTFSNEVVTL-WYRAPDVLLGSR 177 Query: 226 -YSTKSDVY 249 YST D++ Sbjct: 178 VYSTSIDIW 186 >SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1275 Score = 31.1 bits (67), Expect = 0.14 Identities = 15/53 (28%), Positives = 28/53 (52%) Frame = -3 Query: 641 HYFARIFAYEAVLKVNSHLIISILLGKIRPTFTVIASIILVRLLNFVEAFSDV 483 H+ RI A+E V K+N + + PT + + I ++ +LN+ F+D+ Sbjct: 1201 HHLRRIIAFEKVNKMNIRNVCIVF----SPTLNIPSDIFMMLILNYDHIFTDI 1249 >SPAC644.06c |cdr1|nim1|GIN4 family protein kinase Cdr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 593 Score = 30.3 bits (65), Expect = 0.25 Identities = 15/54 (27%), Positives = 29/54 (53%) Frame = +3 Query: 288 ELPFAKXNDSSVLDRLKTGALEWSVPASMPQSVADILKRCWSQSPTDRPQFAEV 449 +LPF N + ++++ GA + +P+S+ + D+L R +P+ R EV Sbjct: 201 KLPFGGQNTDVIYNKIRHGA--YDLPSSISSAAQDLLHRMLDVNPSTRITIPEV 252 >SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 29.1 bits (62), Expect = 0.57 Identities = 19/65 (29%), Positives = 30/65 (46%) Frame = +1 Query: 55 LTHRDIAARNCLITSKLQLKLSCPALTRGPLSREYYKLHDQVIPLRWCPLEVVLEGDYST 234 + HRDI + N L+ + +K++ LS K V W EVV + +Y T Sbjct: 430 IIHRDIKSDNVLLDNSGNIKITDFGFC-ARLSNRTNKRVTMVGTPYWMAPEVVKQNEYGT 488 Query: 235 KSDVY 249 K D++ Sbjct: 489 KVDIW 493 >SPCC18B5.11c |cds1||replication checkpoint kinase Cds1|Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 28.7 bits (61), Expect = 0.75 Identities = 21/70 (30%), Positives = 30/70 (42%), Gaps = 5/70 (7%) Frame = +1 Query: 55 LTHRDIAARNCLITSKLQLKLSCPALTR-----GPLSREYYKLHDQVIPLRWCPLEVVLE 219 +THRDI N LIT+ LK+S L + G + + P V L+ Sbjct: 290 VTHRDIKPENILITNDFHLKISDFGLAKVIHGTGTFLETFCGTMGYLAPEVLKSKNVNLD 349 Query: 220 GDYSTKSDVY 249 G Y K D++ Sbjct: 350 GGYDDKVDIW 359 >SPCC1919.01 |ppk34|SPCC830.12|serine/threonine protein kinase Ppk34|Schizosaccharomyces pombe|chr 3|||Manual Length = 354 Score = 28.7 bits (61), Expect = 0.75 Identities = 18/68 (26%), Positives = 30/68 (44%), Gaps = 1/68 (1%) Frame = +3 Query: 285 AELPFAKXNDSSVLDRLKT-GALEWSVPASMPQSVADILKRCWSQSPTDRPQFAEVCEEI 461 AE PF N S++LD + +PA + +L+RC ++P R E+ + Sbjct: 273 AEFPF---NGSTILDMINVIKGKSLMIPAYCNSDLRKLLERCLEKNPEKRITIEELLRDP 329 Query: 462 NIVPTGDH 485 + G H Sbjct: 330 FLTERGKH 337 >SPAC30C2.08 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 457 Score = 27.9 bits (59), Expect = 1.3 Identities = 12/36 (33%), Positives = 21/36 (58%) Frame = +1 Query: 142 PLSREYYKLHDQVIPLRWCPLEVVLEGDYSTKSDVY 249 P+S E +H+Q+ ++ C LEV G ++ D+Y Sbjct: 354 PVSEELMAVHNQLRTVKRCLLEVQRSGGICSERDLY 389 >SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 27.9 bits (59), Expect = 1.3 Identities = 12/22 (54%), Positives = 16/22 (72%) Frame = +1 Query: 52 RLTHRDIAARNCLITSKLQLKL 117 ++ HRDI N L+TSK Q+KL Sbjct: 201 KIIHRDIKPSNILLTSKGQVKL 222 >SPBC11B10.09 |cdc2|swo2|cyclin-dependent protein kinase Cdc2|Schizosaccharomyces pombe|chr 2|||Manual Length = 297 Score = 27.5 bits (58), Expect = 1.7 Identities = 24/69 (34%), Positives = 36/69 (52%), Gaps = 3/69 (4%) Frame = +1 Query: 52 RLTHRDIAARNCLITSKLQLKLSCPALTR--GPLSREYYKLHDQVIPLRWCPLEVVL-EG 222 R+ HRD+ +N LI + LKL+ L R G R Y H +++ L + EV+L Sbjct: 129 RIIHRDLKPQNLLIDKEGNLKLADFGLARSFGVPLRNY--TH-EIVTLWYRAPEVLLGSR 185 Query: 223 DYSTKSDVY 249 YST D++ Sbjct: 186 HYSTGVDIW 194 >SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc25|Schizosaccharomyces pombe|chr 1|||Manual Length = 596 Score = 27.5 bits (58), Expect = 1.7 Identities = 20/58 (34%), Positives = 32/58 (55%), Gaps = 5/58 (8%) Frame = -1 Query: 349 SAPVF--SRSSTELSFXFAKGSS---ACVYISHTVAATCKHLIWLSSLLQGPLLADTN 191 +AP F SRSS+ S KG+S A ++++ ++ TC +SLL+ L DT+ Sbjct: 195 NAPPFLRSRSSSSYSINKKKGTSGGQATRHLTYALSRTCSQSSNTTSLLESCLTDDTD 252 >SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1463 Score = 26.6 bits (56), Expect = 3.0 Identities = 36/144 (25%), Positives = 64/144 (44%), Gaps = 8/144 (5%) Frame = -3 Query: 608 VLKVNSHLIISILLGKIRPTFTV-IASIILVRLLNFVEAFSDVVSCRDNVDLFADLRELR 432 VL + ++ + L +PT + +AS+++ L F+ A + V+ R N +F L + Sbjct: 77 VLSIITYFLALFLHVVEQPTLRIPMASLLMFWLFKFL-ASALVLLLRPNYTMFPMLNVVP 135 Query: 431 AIRW---RLAPTAFKYV--GNRLWHGGWHAPLQRPGLQPIQHRAIVXFRKRQLGLRVHLP 267 +I + + A YV NR+W+ A L+ GL+P + F + G L Sbjct: 136 SITFFCSLVCLLAEIYVPPANRVWYPDDAAELEETGLRPSRFTYANIFSRISFGWLSPLM 195 Query: 266 HGG--GYM*TSDLVE*SPSRTTSS 201 G Y+ SD P+ +S+ Sbjct: 196 KFGYRNYLTESDAWSLPPAERSSN 219 >SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1338 Score = 25.8 bits (54), Expect = 5.3 Identities = 14/45 (31%), Positives = 22/45 (48%) Frame = +1 Query: 55 LTHRDIAARNCLITSKLQLKLSCPALTRGPLSREYYKLHDQVIPL 189 + HRDI N LI+ LKL+ L+R + + + IP+ Sbjct: 709 IIHRDIKPENLLISQNGHLKLTDFGLSRVGYMKRHRRKQSSSIPV 753 >SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schizosaccharomyces pombe|chr 3|||Manual Length = 576 Score = 25.8 bits (54), Expect = 5.3 Identities = 8/24 (33%), Positives = 15/24 (62%) Frame = +1 Query: 49 HRLTHRDIAARNCLITSKLQLKLS 120 H++ HRD+ N L+ L +K++ Sbjct: 150 HKIVHRDLKPENLLLDDNLNVKIA 173 >SPAC1006.05c |och1||alpha-1,6-mannosyltransferase Och1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 396 Score = 25.8 bits (54), Expect = 5.3 Identities = 13/53 (24%), Positives = 22/53 (41%) Frame = +3 Query: 264 VWEMYTQAELPFAKXNDSSVLDRLKTGALEWSVPASMPQSVADILKRCWSQSP 422 +WE+ + K +DS L + +EW+ P ++ D L W P Sbjct: 290 LWELVRRITDETWKLHDSKKLSKNGESVMEWTGPGIWTDAIMDYLN--WQYGP 340 >SPBC409.07c |wis1|spc2, smf2|MAP kinase kinase Wis1|Schizosaccharomyces pombe|chr 2|||Manual Length = 605 Score = 25.4 bits (53), Expect = 7.0 Identities = 9/31 (29%), Positives = 18/31 (58%) Frame = +3 Query: 357 SVPASMPQSVADILKRCWSQSPTDRPQFAEV 449 S+P S D + +C +++P+ RP + E+ Sbjct: 543 SLPDSFSPEARDFVNKCLNKNPSLRPDYHEL 573 >SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr 3|||Manual Length = 625 Score = 25.4 bits (53), Expect = 7.0 Identities = 21/88 (23%), Positives = 38/88 (43%), Gaps = 2/88 (2%) Frame = -1 Query: 397 SMSATDCGMEAGTLHSSAPVFSRSSTELSFXFAKGSSACVYISHTVAATCK--HLIWLSS 224 S+S+ + SS+ SRS + S GS++ SHT+ +T + H S+ Sbjct: 518 SLSSVHTSSATSSSKSSSSSSSRSGSSSSSSSRSGSTSSSGSSHTITSTSQSVHTSGSST 577 Query: 223 LLQGPLLADTNGEGSPGRAACSTRETAA 140 + T S ++ S+ E++A Sbjct: 578 STSSVAVTSTAYSSSSSSSSSSSIESSA 605 >SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1297 Score = 25.0 bits (52), Expect = 9.3 Identities = 10/20 (50%), Positives = 15/20 (75%) Frame = -3 Query: 515 LLNFVEAFSDVVSCRDNVDL 456 LLN +EAF+ + RD++DL Sbjct: 882 LLNLIEAFNKARALRDSLDL 901 >SPAC1D4.13 |byr1|ste1, ste3|MAP kinase kinase Byr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 340 Score = 25.0 bits (52), Expect = 9.3 Identities = 21/69 (30%), Positives = 35/69 (50%), Gaps = 5/69 (7%) Frame = +3 Query: 261 TVWEMYTQAELP--FAKXNDS-SVLDRLKTGALEWS--VPASMPQSVADILKRCWSQSPT 425 ++ E+ TQ ELP F+ +DS +LD L E +P+S P+ + + C + PT Sbjct: 248 SIIELATQ-ELPWSFSNIDDSIGILDLLHCIVQEEPPRLPSSFPEDLRLFVDACLHKDPT 306 Query: 426 DRPQFAEVC 452 R ++C Sbjct: 307 LRASPQQLC 315 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,367,067 Number of Sequences: 5004 Number of extensions: 47706 Number of successful extensions: 150 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 143 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 287744314 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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