Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30265
(583 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC328.10c |rps502|rps5-2|40S ribosomal protein S5|Schizosaccha... 173 2e-44
SPAC8C9.08 |rps5||40S ribosomal protein S5|Schizosaccharomyces p... 173 2e-44
SPAC16E8.10c |||mitochondrial ribosomal protein subunit S7|Schiz... 36 0.006
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 0.50
SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe... 27 2.0
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 27 2.6
SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual 25 6.1
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 25 8.1
>SPAC328.10c |rps502|rps5-2|40S ribosomal protein
S5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 203
Score = 173 bits (420), Expect = 2e-44
Identities = 84/109 (77%), Positives = 96/109 (88%)
Frame = +3
Query: 255 RNNGKKLMAVRIVKHAFXIIHLLTGENPLQVLVTAIINSGPREDSTRIGRAGTVRRQAVD 434
RNNGKKL+A RIVKHAF II LLT +NPLQVLV A+ GPREDSTRIG AGTVRRQAVD
Sbjct: 80 RNNGKKLLATRIVKHAFEIIALLTDQNPLQVLVDAVAACGPREDSTRIGSAGTVRRQAVD 139
Query: 435 VSPLRRVNQAIWLLCTGAREAAFQNIXTIAECVADELINAAKGSSNSYA 581
VSPLRRVNQA+ L+ GAREAAF+N+ +I+EC+A+E+INAAKGSSNSYA
Sbjct: 140 VSPLRRVNQALALITIGAREAAFRNVKSISECLAEEIINAAKGSSNSYA 188
Score = 76.6 bits (180), Expect = 2e-15
Identities = 42/88 (47%), Positives = 58/88 (65%)
Frame = +1
Query: 7 AGSVVVETMSLPQAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYA 186
A S++ + +SL + IKLF ++ V+V D+SL DYI++ + LPH+AGR+
Sbjct: 2 AASIIPKEVSLDETG---HIKLFNKFPFEGVEVKDISLVDYITIGN--GQPLPHTAGRFQ 56
Query: 187 HKRFRKAQCPIVERLTNSLMMHGGTMAK 270
KRFRKA+C IVERLTNSLMM+G K
Sbjct: 57 TKRFRKARCFIVERLTNSLMMNGRNNGK 84
>SPAC8C9.08 |rps5||40S ribosomal protein S5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 203
Score = 173 bits (420), Expect = 2e-44
Identities = 84/109 (77%), Positives = 96/109 (88%)
Frame = +3
Query: 255 RNNGKKLMAVRIVKHAFXIIHLLTGENPLQVLVTAIINSGPREDSTRIGRAGTVRRQAVD 434
RNNGKKL+A RIVKHAF II LLT +NPLQVLV A+ GPREDSTRIG AGTVRRQAVD
Sbjct: 80 RNNGKKLLATRIVKHAFEIIALLTDQNPLQVLVDAVAACGPREDSTRIGSAGTVRRQAVD 139
Query: 435 VSPLRRVNQAIWLLCTGAREAAFQNIXTIAECVADELINAAKGSSNSYA 581
VSPLRRVNQA+ L+ GAREAAF+N+ +I+EC+A+E+INAAKGSSNSYA
Sbjct: 140 VSPLRRVNQALALITIGAREAAFRNVKSISECLAEEIINAAKGSSNSYA 188
Score = 74.1 bits (174), Expect = 1e-14
Identities = 42/84 (50%), Positives = 54/84 (64%), Gaps = 3/84 (3%)
Frame = +1
Query: 28 TMSLPQAADIPE---IKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYAHKRF 198
T SL + E IKLF ++ V+V D+SL DYI++ + LPH+AGR+ KRF
Sbjct: 3 TSSLTPGVSLDENGSIKLFNKFPFEGVEVKDISLVDYITIGN--GQPLPHTAGRFQTKRF 60
Query: 199 RKAQCPIVERLTNSLMMHGGTMAK 270
RKA+C IVERLTNSLMM+G K
Sbjct: 61 RKARCFIVERLTNSLMMNGRNNGK 84
>SPAC16E8.10c |||mitochondrial ribosomal protein subunit
S7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 259
Score = 35.5 bits (78), Expect = 0.006
Identities = 19/39 (48%), Positives = 22/39 (56%)
Frame = +3
Query: 261 NGKKLMAVRIVKHAFXIIHLLTGENPLQVLVTAIINSGP 377
+GKK A +IV A II TGENP+ VL AI P
Sbjct: 136 DGKKAKAEKIVATALSIIQKETGENPIDVLKQAIAEISP 174
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 29.1 bits (62), Expect = 0.50
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = +2
Query: 278 GRTYCQTCV*XYSLVNWRKPSASTRDCHYQLWT 376
G TYC C L+NW K S S C +L+T
Sbjct: 101 GHTYCYEC-----LLNWLKESKSCPTCRQKLYT 128
>SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 27.1 bits (57), Expect = 2.0
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +1
Query: 43 QAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYA 186
+ ADI + FGR ++++++ + I+V+EKYAK P R A
Sbjct: 7 KVADI-SLAAFGR---KELEIAENEMPGLIAVREKYAKSQPLKGARIA 50
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 26.6 bits (56), Expect = 2.6
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +1
Query: 370 LDPVKIRLGSVVRVQFVVKPLMFHPCAESTKQSGFCAQV 486
++PV R SVV V +P+ +H +S ++G Q+
Sbjct: 273 IEPVSSRQSSVVNNNSVQQPVAYHAFVQSPTENGTLPQL 311
>SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual
Length = 613
Score = 25.4 bits (53), Expect = 6.1
Identities = 16/54 (29%), Positives = 28/54 (51%)
Frame = -1
Query: 205 LYGNACVHTCQLNEVNILRTSL*RKCSPAETYQRLAHRSNSICRKA*FQECRRL 44
L N C+ LNE ++ + SL R P++ ++ R +S+ +K+ E R L
Sbjct: 147 LLNNCCIGIANLNEDSVHKISLTRSGKPSQLV-TVSCRHSSVIQKSYGSEKRYL 199
>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 131
Score = 25.0 bits (52), Expect = 8.1
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = +2
Query: 32 CLYHKPPTFLKSSFS 76
C H P TFL SSFS
Sbjct: 117 CFIHPPITFLSSSFS 131
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,442,471
Number of Sequences: 5004
Number of extensions: 48156
Number of successful extensions: 115
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 250133048
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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