BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30265 (583 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g37270.1 68415.m04572 40S ribosomal protein S5 (RPS5A) identi... 186 7e-48 At3g11940.2 68416.m01470 40S ribosomal protein S5 (RPS5B) simila... 186 1e-47 At3g11940.1 68416.m01469 40S ribosomal protein S5 (RPS5B) simila... 186 1e-47 At4g39450.1 68417.m05582 expressed protein 28 4.0 At5g01940.1 68418.m00113 eukaryotic translation initiation facto... 27 6.9 At4g39955.1 68417.m05659 hydrolase, alpha/beta fold family prote... 27 6.9 At1g57600.1 68414.m06536 membrane bound O-acyl transferase (MBOA... 27 6.9 >At2g37270.1 68415.m04572 40S ribosomal protein S5 (RPS5A) identical to GP:3043428 Length = 207 Score = 186 bits (454), Expect = 7e-48 Identities = 90/109 (82%), Positives = 100/109 (91%) Frame = +3 Query: 255 RNNGKKLMAVRIVKHAFXIIHLLTGENPLQVLVTAIINSGPREDSTRIGRAGTVRRQAVD 434 RNNGKKLMAVRIVKHA IIHLL+ NP+QV++ AI+NSGPRED+TRIG AG VRRQAVD Sbjct: 84 RNNGKKLMAVRIVKHAMEIIHLLSDLNPIQVIIDAIVNSGPREDATRIGSAGVVRRQAVD 143 Query: 435 VSPLRRVNQAIWLLCTGAREAAFQNIXTIAECVADELINAAKGSSNSYA 581 +SPLRRVNQAI+LL TGAREAAF+NI TIAEC+ADELINAAKGSSNSYA Sbjct: 144 ISPLRRVNQAIFLLTTGAREAAFRNIKTIAECLADELINAAKGSSNSYA 192 Score = 102 bits (244), Expect = 2e-22 Identities = 48/71 (67%), Positives = 57/71 (80%), Gaps = 1/71 (1%) Frame = +1 Query: 61 EIKLFGRWSCYDVQVSDMSLQDYISVK-EKYAKYLPHSAGRYAHKRFRKAQCPIVERLTN 237 E+KLF RWS DV V+D+SL DYI V+ K+A ++PH+AGRY+ KRFRKAQCPIVERLTN Sbjct: 18 EVKLFNRWSFDDVSVTDISLVDYIGVQPSKHATFVPHTAGRYSVKRFRKAQCPIVERLTN 77 Query: 238 SLMMHGGTMAK 270 SLMMHG K Sbjct: 78 SLMMHGRNNGK 88 >At3g11940.2 68416.m01470 40S ribosomal protein S5 (RPS5B) similar to 40S ribosomal protein S5 GB:AAC98068 GI:4056502 from [Arabidopsis thaliana] Length = 207 Score = 186 bits (452), Expect = 1e-47 Identities = 89/109 (81%), Positives = 100/109 (91%) Frame = +3 Query: 255 RNNGKKLMAVRIVKHAFXIIHLLTGENPLQVLVTAIINSGPREDSTRIGRAGTVRRQAVD 434 RNNGKKLMAVRIVKHA IIHLL+ NP+QV++ AI+NSGPRED+TRIG AG VRRQAVD Sbjct: 84 RNNGKKLMAVRIVKHAMEIIHLLSDLNPIQVIIDAIVNSGPREDATRIGSAGVVRRQAVD 143 Query: 435 VSPLRRVNQAIWLLCTGAREAAFQNIXTIAECVADELINAAKGSSNSYA 581 +SPLRRVNQAI+L+ TGAREAAF+NI TIAEC+ADELINAAKGSSNSYA Sbjct: 144 ISPLRRVNQAIFLITTGAREAAFRNIKTIAECLADELINAAKGSSNSYA 192 Score = 100 bits (240), Expect = 6e-22 Identities = 49/77 (63%), Positives = 59/77 (76%), Gaps = 1/77 (1%) Frame = +1 Query: 43 QAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKE-KYAKYLPHSAGRYAHKRFRKAQCPI 219 Q A E+KLF RW+ DV V+D+SL DYI V+ K+A ++PH+AGRY+ KRFRKAQCPI Sbjct: 12 QQALTNEVKLFNRWTYDDVTVTDISLVDYIGVQAAKHATFVPHTAGRYSVKRFRKAQCPI 71 Query: 220 VERLTNSLMMHGGTMAK 270 VERLTNSLMMHG K Sbjct: 72 VERLTNSLMMHGRNNGK 88 >At3g11940.1 68416.m01469 40S ribosomal protein S5 (RPS5B) similar to 40S ribosomal protein S5 GB:AAC98068 GI:4056502 from [Arabidopsis thaliana] Length = 207 Score = 186 bits (452), Expect = 1e-47 Identities = 89/109 (81%), Positives = 100/109 (91%) Frame = +3 Query: 255 RNNGKKLMAVRIVKHAFXIIHLLTGENPLQVLVTAIINSGPREDSTRIGRAGTVRRQAVD 434 RNNGKKLMAVRIVKHA IIHLL+ NP+QV++ AI+NSGPRED+TRIG AG VRRQAVD Sbjct: 84 RNNGKKLMAVRIVKHAMEIIHLLSDLNPIQVIIDAIVNSGPREDATRIGSAGVVRRQAVD 143 Query: 435 VSPLRRVNQAIWLLCTGAREAAFQNIXTIAECVADELINAAKGSSNSYA 581 +SPLRRVNQAI+L+ TGAREAAF+NI TIAEC+ADELINAAKGSSNSYA Sbjct: 144 ISPLRRVNQAIFLITTGAREAAFRNIKTIAECLADELINAAKGSSNSYA 192 Score = 100 bits (240), Expect = 6e-22 Identities = 49/77 (63%), Positives = 59/77 (76%), Gaps = 1/77 (1%) Frame = +1 Query: 43 QAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKE-KYAKYLPHSAGRYAHKRFRKAQCPI 219 Q A E+KLF RW+ DV V+D+SL DYI V+ K+A ++PH+AGRY+ KRFRKAQCPI Sbjct: 12 QQALTNEVKLFNRWTYDDVTVTDISLVDYIGVQAAKHATFVPHTAGRYSVKRFRKAQCPI 71 Query: 220 VERLTNSLMMHGGTMAK 270 VERLTNSLMMHG K Sbjct: 72 VERLTNSLMMHGRNNGK 88 >At4g39450.1 68417.m05582 expressed protein Length = 1553 Score = 28.3 bits (60), Expect = 4.0 Identities = 21/48 (43%), Positives = 26/48 (54%) Frame = +3 Query: 432 DVSPLRRVNQAIWLLCTGAREAAFQNIXTIAECVADELINAAKGSSNS 575 DVSPL + IWL T ARE + + I +A E I AA S+NS Sbjct: 411 DVSPLSCLT--IWLEITAARETSSIKVNDITTKIA-ENIGAAVVSTNS 455 >At5g01940.1 68418.m00113 eukaryotic translation initiation factor 2B family protein / eIF-2B family protein similar to SP|P41035 Eukaryotic translation initiation factor 2 subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains Pfam profile PF01873: Domain found in IF2B/IF5 Length = 231 Score = 27.5 bits (58), Expect = 6.9 Identities = 15/42 (35%), Positives = 22/42 (52%) Frame = +2 Query: 77 ADGVATMCKSLICLCRTTFPLKRSTQNIYLIQLAGMHTSVSV 202 A+G T+C + LCRT + R ++ LA M T VS+ Sbjct: 109 AEGTITVCLNFADLCRT---MHRKPDHVMKFLLAQMETKVSL 147 >At4g39955.1 68417.m05659 hydrolase, alpha/beta fold family protein low similarity to SP|P24640|LIP3_MORSP Lipase 3 precursor (EC 3.1.1.3) (Triacylglycerol lipase) {Moraxella sp}; contains Pfam profile PF00561: hydrolase, alpha/beta fold family Length = 328 Score = 27.5 bits (58), Expect = 6.9 Identities = 9/25 (36%), Positives = 16/25 (64%) Frame = +2 Query: 38 YHKPPTFLKSSFSADGVATMCKSLI 112 ++KPP ++ S F+ D + MCK + Sbjct: 191 FYKPPIWIPSCFAMDYIHVMCKDYL 215 >At1g57600.1 68414.m06536 membrane bound O-acyl transferase (MBOAT) family protein low similarity to skinny hedgehog [Drosophila melanogaster] GI:15420842; contains Pfam profile PF03062: MBOAT family Length = 533 Score = 27.5 bits (58), Expect = 6.9 Identities = 10/22 (45%), Positives = 13/22 (59%) Frame = +2 Query: 14 AWLWKPCLYHKPPTFLKSSFSA 79 +W W CL+ P LKS+ SA Sbjct: 426 SWAWLTCLFFMPEMLLKSASSA 447 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,030,475 Number of Sequences: 28952 Number of extensions: 264356 Number of successful extensions: 581 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 574 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 578 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1141585696 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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