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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30263
         (687 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_UPI000051AACF Cluster: PREDICTED: similar to CG14435-PA...   105   1e-21
UniRef50_Q9W3V3 Cluster: CG14435-PA; n=3; Diptera|Rep: CG14435-P...   105   1e-21
UniRef50_UPI00015B5DB3 Cluster: PREDICTED: similar to CG14435-PA...   103   5e-21
UniRef50_Q8NHG8 Cluster: E3 ubiquitin-protein ligase ZNRF2; n=15...    71   4e-11
UniRef50_A7S6Y6 Cluster: Predicted protein; n=2; Eumetazoa|Rep: ...    69   8e-11
UniRef50_Q08CN9 Cluster: E3 ubiquitin-protein ligase ZNRF2; n=5;...    65   1e-09
UniRef50_UPI000155E0E6 Cluster: PREDICTED: hypothetical protein;...    64   4e-09
UniRef50_Q8ND25 Cluster: E3 ubiquitin-protein ligase ZNRF1; n=14...    60   5e-08
UniRef50_UPI0000E47BD5 Cluster: PREDICTED: hypothetical protein;...    59   1e-07
UniRef50_Q9N4I6 Cluster: Putative uncharacterized protein; n=2; ...    59   1e-07
UniRef50_A7E2J2 Cluster: Putative uncharacterized protein; n=3; ...    58   2e-07
UniRef50_Q4SIQ9 Cluster: Chromosome 21 SCAF14577, whole genome s...    46   9e-04
UniRef50_Q6PFI9 Cluster: Zgc:66427; n=3; Clupeocephala|Rep: Zgc:...    38   0.30 
UniRef50_UPI0000DB6D3A Cluster: PREDICTED: similar to T19B10.6; ...    35   2.1  
UniRef50_UPI0000D57429 Cluster: PREDICTED: similar to membrane-a...    35   2.1  
UniRef50_Q4RYQ4 Cluster: Chromosome 16 SCAF14974, whole genome s...    35   2.1  
UniRef50_Q0UXD0 Cluster: Putative uncharacterized protein; n=1; ...    34   2.8  
UniRef50_UPI00015B4FA7 Cluster: PREDICTED: hypothetical protein;...    33   6.5  
UniRef50_UPI0000EB2565 Cluster: UPI0000EB2565 related cluster; n...    33   6.5  

>UniRef50_UPI000051AACF Cluster: PREDICTED: similar to CG14435-PA;
           n=2; Endopterygota|Rep: PREDICTED: similar to CG14435-PA
           - Apis mellifera
          Length = 212

 Score =  105 bits (252), Expect = 1e-21
 Identities = 44/58 (75%), Positives = 50/58 (86%)
 Frame = +1

Query: 484 SGPCLRSXPLPSHIWSINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLSDSK 657
           SG    +  LPSHIWS+NG+KCPV SKF+LPDDIECHLVMCLT+PRLSYNED+LSD K
Sbjct: 109 SGRVYAAHSLPSHIWSLNGLKCPVCSKFILPDDIECHLVMCLTKPRLSYNEDILSDEK 166


>UniRef50_Q9W3V3 Cluster: CG14435-PA; n=3; Diptera|Rep: CG14435-PA -
           Drosophila melanogaster (Fruit fly)
          Length = 303

 Score =  105 bits (252), Expect = 1e-21
 Identities = 46/59 (77%), Positives = 50/59 (84%)
 Frame = +1

Query: 481 GSGPCLRSXPLPSHIWSINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLSDSK 657
           G G    +  LPSHIWS NGIKCPV +KFVLPDDIECHLVMCLT+PRLSYNEDVLSD+K
Sbjct: 199 GIGRVYTATSLPSHIWSFNGIKCPVCNKFVLPDDIECHLVMCLTKPRLSYNEDVLSDAK 257



 Score = 41.1 bits (92), Expect = 0.025
 Identities = 23/43 (53%), Positives = 29/43 (67%), Gaps = 7/43 (16%)
 Frame = +2

Query: 248 GRVSGAEGFSLLRAIPGIQV-------SNXRQRARSLSSVPDL 355
           G  +  +GF+LLR +PG+QV       S  RQRARSLSSVPD+
Sbjct: 56  GNDTTGQGFNLLRTLPGLQVYHNQNSTSIDRQRARSLSSVPDI 98



 Score = 32.7 bits (71), Expect = 8.6
 Identities = 17/25 (68%), Positives = 20/25 (80%), Gaps = 2/25 (8%)
 Frame = +3

Query: 174 MGAKAST--ANGGQSPRARTFSTSS 242
           MG KAST   +G QSPR+RTFS+SS
Sbjct: 1   MGQKASTPATSGQQSPRSRTFSSSS 25


>UniRef50_UPI00015B5DB3 Cluster: PREDICTED: similar to CG14435-PA;
           n=1; Nasonia vitripennis|Rep: PREDICTED: similar to
           CG14435-PA - Nasonia vitripennis
          Length = 268

 Score =  103 bits (246), Expect = 5e-21
 Identities = 42/49 (85%), Positives = 47/49 (95%)
 Frame = +1

Query: 511 LPSHIWSINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLSDSK 657
           LPSHIWS+NG+KCPV SKF+LPDDIECHLVMCLT+PRLSYNEDVL+D K
Sbjct: 174 LPSHIWSLNGLKCPVCSKFILPDDIECHLVMCLTKPRLSYNEDVLADEK 222


>UniRef50_Q8NHG8 Cluster: E3 ubiquitin-protein ligase ZNRF2; n=15;
           Euteleostomi|Rep: E3 ubiquitin-protein ligase ZNRF2 -
           Homo sapiens (Human)
          Length = 242

 Score = 70.5 bits (165), Expect = 4e-11
 Identities = 35/62 (56%), Positives = 43/62 (69%), Gaps = 2/62 (3%)
 Frame = +1

Query: 469 GGVAGSGPCLRSXPLPSHI--WSINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDV 642
           GG  G GP L    LP+H+      G KCPV SKFV  D+++ HLVMCLT+PR++YNEDV
Sbjct: 133 GGSPG-GPRLVIGSLPAHLSPHMFGGFKCPVCSKFVSSDEMDLHLVMCLTKPRITYNEDV 191

Query: 643 LS 648
           LS
Sbjct: 192 LS 193


>UniRef50_A7S6Y6 Cluster: Predicted protein; n=2; Eumetazoa|Rep:
           Predicted protein - Nematostella vectensis
          Length = 85

 Score = 69.3 bits (162), Expect = 8e-11
 Identities = 29/40 (72%), Positives = 34/40 (85%)
 Frame = +1

Query: 538 GIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLSDSK 657
           G+KCPV +K VLP DIE HLV+CLT+PRLSYNEDVL + K
Sbjct: 1   GLKCPVCAKVVLPQDIELHLVICLTKPRLSYNEDVLQEDK 40


>UniRef50_Q08CN9 Cluster: E3 ubiquitin-protein ligase ZNRF2; n=5;
           Euteleostomi|Rep: E3 ubiquitin-protein ligase ZNRF2 -
           Danio rerio (Zebrafish) (Brachydanio rerio)
          Length = 217

 Score = 65.3 bits (152), Expect = 1e-09
 Identities = 30/55 (54%), Positives = 39/55 (70%), Gaps = 2/55 (3%)
 Frame = +1

Query: 490 PCLRSXPLPSHIWS--INGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648
           P L    LP+H+      G KCPV SKF+  D+++ HLVMCLT+PR++YNEDVLS
Sbjct: 114 PRLVIGSLPAHLSPHLFGGFKCPVCSKFISSDEMDLHLVMCLTKPRVTYNEDVLS 168


>UniRef50_UPI000155E0E6 Cluster: PREDICTED: hypothetical protein;
           n=1; Equus caballus|Rep: PREDICTED: hypothetical protein
           - Equus caballus
          Length = 214

 Score = 63.7 bits (148), Expect = 4e-09
 Identities = 26/38 (68%), Positives = 33/38 (86%)
 Frame = +1

Query: 535 NGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648
           +G KCPV SKFV  D+++ HLVMCLT+PR++YNEDVLS
Sbjct: 128 SGFKCPVCSKFVPSDEMDLHLVMCLTKPRITYNEDVLS 165


>UniRef50_Q8ND25 Cluster: E3 ubiquitin-protein ligase ZNRF1; n=14;
           Euteleostomi|Rep: E3 ubiquitin-protein ligase ZNRF1 -
           Homo sapiens (Human)
          Length = 227

 Score = 60.1 bits (139), Expect = 5e-08
 Identities = 28/51 (54%), Positives = 37/51 (72%), Gaps = 5/51 (9%)
 Frame = +1

Query: 511 LPSHI---W--SINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648
           LP HI   W  S +G KCP+ SK V  D++E H +MCL++PRLSYN+DVL+
Sbjct: 128 LPLHIAPRWFSSHSGFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLT 178


>UniRef50_UPI0000E47BD5 Cluster: PREDICTED: hypothetical protein;
           n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
           hypothetical protein - Strongylocentrotus purpuratus
          Length = 213

 Score = 58.8 bits (136), Expect = 1e-07
 Identities = 32/60 (53%), Positives = 38/60 (63%)
 Frame = +1

Query: 469 GGVAGSGPCLRSXPLPSHIWSINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648
           GG +GSG    S    S       IKCPV SKFV  D+IE HL+ CLT+PR+ YNEDVL+
Sbjct: 106 GGGSGSGSST-SGASGSGGGGGRSIKCPVCSKFVGADNIELHLLACLTKPRIVYNEDVLT 164


>UniRef50_Q9N4I6 Cluster: Putative uncharacterized protein; n=2;
           Caenorhabditis|Rep: Putative uncharacterized protein -
           Caenorhabditis elegans
          Length = 189

 Score = 58.8 bits (136), Expect = 1e-07
 Identities = 24/39 (61%), Positives = 30/39 (76%)
 Frame = +1

Query: 541 IKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLSDSK 657
           +KCPV  K V  DD + HLVMCLTRP+++YN+DVL D K
Sbjct: 104 MKCPVCHKVVPSDDADIHLVMCLTRPKITYNDDVLKDDK 142


>UniRef50_A7E2J2 Cluster: Putative uncharacterized protein; n=3;
           Euteleostomi|Rep: Putative uncharacterized protein -
           Danio rerio (Zebrafish) (Brachydanio rerio)
          Length = 181

 Score = 58.0 bits (134), Expect = 2e-07
 Identities = 25/46 (54%), Positives = 33/46 (71%)
 Frame = +1

Query: 511 LPSHIWSINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648
           L  H+  + G  CPV SKFV  D+I+ HL+MC ++PRL YN+DVLS
Sbjct: 88  LTPHLLGV-GFDCPVCSKFVCSDEIDVHLLMCFSKPRLHYNDDVLS 132


>UniRef50_Q4SIQ9 Cluster: Chromosome 21 SCAF14577, whole genome
           shotgun sequence; n=2; Tetraodontidae|Rep: Chromosome 21
           SCAF14577, whole genome shotgun sequence - Tetraodon
           nigroviridis (Green puffer)
          Length = 205

 Score = 46.0 bits (104), Expect = 9e-04
 Identities = 17/34 (50%), Positives = 27/34 (79%)
 Frame = +1

Query: 547 CPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648
           CP+ +KF+  D+IE HL+ C ++ RL+YN+D+LS
Sbjct: 123 CPLCAKFMATDEIEKHLLKCFSKMRLTYNKDILS 156


>UniRef50_Q6PFI9 Cluster: Zgc:66427; n=3; Clupeocephala|Rep:
           Zgc:66427 - Danio rerio (Zebrafish) (Brachydanio rerio)
          Length = 254

 Score = 37.5 bits (83), Expect = 0.30
 Identities = 18/36 (50%), Positives = 22/36 (61%)
 Frame = +1

Query: 541 IKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648
           I+CP  +K      IE HL+ CLT P L YN DVL+
Sbjct: 170 IRCPFCTKPFPGGRIEDHLLSCLTSPPLPYNTDVLA 205


>UniRef50_UPI0000DB6D3A Cluster: PREDICTED: similar to T19B10.6;
           n=1; Apis mellifera|Rep: PREDICTED: similar to T19B10.6
           - Apis mellifera
          Length = 472

 Score = 34.7 bits (76), Expect = 2.1
 Identities = 16/51 (31%), Positives = 27/51 (52%), Gaps = 2/51 (3%)
 Frame = +1

Query: 541 IKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLSDSKRRMCN--MPRGV 687
           +KCP+ + F+L D I  H+         + NE ++S S   +CN  +P G+
Sbjct: 354 VKCPICNNFILNDKINEHIDFYDIESNRNVNESIISISSTSICNELIPIGI 404


>UniRef50_UPI0000D57429 Cluster: PREDICTED: similar to
           membrane-associated ring finger (C3HC4) 3; n=1;
           Tribolium castaneum|Rep: PREDICTED: similar to
           membrane-associated ring finger (C3HC4) 3 - Tribolium
           castaneum
          Length = 255

 Score = 34.7 bits (76), Expect = 2.1
 Identities = 23/70 (32%), Positives = 36/70 (51%)
 Frame = -2

Query: 233 RECPCARTLPPVRSARLCTHNDSVTQIANMSCTETEDHENSRDSADAPAERIVRSTETIA 54
           REC   R +PP  +A +C H  S  + AN + T TED+E++R   D+  E+     E   
Sbjct: 181 REC-VVRYIPP-STAHICCH--SPLETANPNNTPTEDNEDARSRNDSEVEQNNEEVEQGG 236

Query: 53  RLIKLALEIP 24
               + +E+P
Sbjct: 237 SEPTVVIEMP 246


>UniRef50_Q4RYQ4 Cluster: Chromosome 16 SCAF14974, whole genome
           shotgun sequence; n=2; Tetraodontidae|Rep: Chromosome 16
           SCAF14974, whole genome shotgun sequence - Tetraodon
           nigroviridis (Green puffer)
          Length = 282

 Score = 34.7 bits (76), Expect = 2.1
 Identities = 18/47 (38%), Positives = 28/47 (59%)
 Frame = -3

Query: 301 NPWDGSQQAKAFRARHTSDPELVENVLARGLCPPFAVLAFAPITTPL 161
           +P D  QQ  +  A +++ P L  +V  + LCP +A+L +AP  TPL
Sbjct: 85  SPADCGQQDGSAAAAYSAAPALAADVYMQTLCPSYAMLTYAP--TPL 129


>UniRef50_Q0UXD0 Cluster: Putative uncharacterized protein; n=1;
           Phaeosphaeria nodorum|Rep: Putative uncharacterized
           protein - Phaeosphaeria nodorum (Septoria nodorum)
          Length = 1186

 Score = 34.3 bits (75), Expect = 2.8
 Identities = 28/85 (32%), Positives = 37/85 (43%), Gaps = 1/85 (1%)
 Frame = -2

Query: 488 PEPATPPXRHQFPHLN*NLCRXXQLQKPHSSASSGVWLLLREKRAGPVLKTETERAAXHS 309
           P PA  P  HQ  HL   + R   + +P +  S+   L+L  + AGP +  E       +
Sbjct: 338 PRPAQRPLFHQ--HLPYPVARVFDVARPANDDSAQAPLVLLPQPAGPKVVEEKANQVWLN 395

Query: 308 TLESLGWLATS-*SLPRPTHVRPGA 237
           T ES  W A S  S P  T   P A
Sbjct: 396 TTESGAWYALSEISYPAVTDGAPTA 420


>UniRef50_UPI00015B4FA7 Cluster: PREDICTED: hypothetical protein;
           n=1; Nasonia vitripennis|Rep: PREDICTED: hypothetical
           protein - Nasonia vitripennis
          Length = 668

 Score = 33.1 bits (72), Expect = 6.5
 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
 Frame = +1

Query: 541 IKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS--DSKRRMCNM 675
           I CP+ SK +LP +   HL  CL+   L   +DV+S  DS     N+
Sbjct: 534 IDCPMCSKKLLPVNFNDHLQKCLSSDMLEEEDDVVSLHDSSSSSINL 580


>UniRef50_UPI0000EB2565 Cluster: UPI0000EB2565 related cluster; n=1;
           Canis lupus familiaris|Rep: UPI0000EB2565 UniRef100
           entry - Canis familiaris
          Length = 526

 Score = 33.1 bits (72), Expect = 6.5
 Identities = 25/74 (33%), Positives = 31/74 (41%), Gaps = 1/74 (1%)
 Frame = -2

Query: 326 RAAXHSTLESLGWLATS*SLPRPTHVRP-GASRECPCARTLPPVRSARLCTHNDSVTQIA 150
           R A H+ L + G   T+     P    P G+SR  P AR L     ARL TH  S     
Sbjct: 102 RTAPHARLPTHGSSRTAPPHGSPRTAPPLGSSRTAPHARLLRTAPHARLLTHGSSARLPT 161

Query: 149 NMSCTETEDHENSR 108
           + S      H +SR
Sbjct: 162 HGSSARLPTHGSSR 175


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 629,511,040
Number of Sequences: 1657284
Number of extensions: 11924288
Number of successful extensions: 35806
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 34367
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35788
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 53719013270
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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