BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30263 (687 letters) Database: uniref50 1,657,284 sequences; 575,637,011 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value UniRef50_UPI000051AACF Cluster: PREDICTED: similar to CG14435-PA... 105 1e-21 UniRef50_Q9W3V3 Cluster: CG14435-PA; n=3; Diptera|Rep: CG14435-P... 105 1e-21 UniRef50_UPI00015B5DB3 Cluster: PREDICTED: similar to CG14435-PA... 103 5e-21 UniRef50_Q8NHG8 Cluster: E3 ubiquitin-protein ligase ZNRF2; n=15... 71 4e-11 UniRef50_A7S6Y6 Cluster: Predicted protein; n=2; Eumetazoa|Rep: ... 69 8e-11 UniRef50_Q08CN9 Cluster: E3 ubiquitin-protein ligase ZNRF2; n=5;... 65 1e-09 UniRef50_UPI000155E0E6 Cluster: PREDICTED: hypothetical protein;... 64 4e-09 UniRef50_Q8ND25 Cluster: E3 ubiquitin-protein ligase ZNRF1; n=14... 60 5e-08 UniRef50_UPI0000E47BD5 Cluster: PREDICTED: hypothetical protein;... 59 1e-07 UniRef50_Q9N4I6 Cluster: Putative uncharacterized protein; n=2; ... 59 1e-07 UniRef50_A7E2J2 Cluster: Putative uncharacterized protein; n=3; ... 58 2e-07 UniRef50_Q4SIQ9 Cluster: Chromosome 21 SCAF14577, whole genome s... 46 9e-04 UniRef50_Q6PFI9 Cluster: Zgc:66427; n=3; Clupeocephala|Rep: Zgc:... 38 0.30 UniRef50_UPI0000DB6D3A Cluster: PREDICTED: similar to T19B10.6; ... 35 2.1 UniRef50_UPI0000D57429 Cluster: PREDICTED: similar to membrane-a... 35 2.1 UniRef50_Q4RYQ4 Cluster: Chromosome 16 SCAF14974, whole genome s... 35 2.1 UniRef50_Q0UXD0 Cluster: Putative uncharacterized protein; n=1; ... 34 2.8 UniRef50_UPI00015B4FA7 Cluster: PREDICTED: hypothetical protein;... 33 6.5 UniRef50_UPI0000EB2565 Cluster: UPI0000EB2565 related cluster; n... 33 6.5 >UniRef50_UPI000051AACF Cluster: PREDICTED: similar to CG14435-PA; n=2; Endopterygota|Rep: PREDICTED: similar to CG14435-PA - Apis mellifera Length = 212 Score = 105 bits (252), Expect = 1e-21 Identities = 44/58 (75%), Positives = 50/58 (86%) Frame = +1 Query: 484 SGPCLRSXPLPSHIWSINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLSDSK 657 SG + LPSHIWS+NG+KCPV SKF+LPDDIECHLVMCLT+PRLSYNED+LSD K Sbjct: 109 SGRVYAAHSLPSHIWSLNGLKCPVCSKFILPDDIECHLVMCLTKPRLSYNEDILSDEK 166 >UniRef50_Q9W3V3 Cluster: CG14435-PA; n=3; Diptera|Rep: CG14435-PA - Drosophila melanogaster (Fruit fly) Length = 303 Score = 105 bits (252), Expect = 1e-21 Identities = 46/59 (77%), Positives = 50/59 (84%) Frame = +1 Query: 481 GSGPCLRSXPLPSHIWSINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLSDSK 657 G G + LPSHIWS NGIKCPV +KFVLPDDIECHLVMCLT+PRLSYNEDVLSD+K Sbjct: 199 GIGRVYTATSLPSHIWSFNGIKCPVCNKFVLPDDIECHLVMCLTKPRLSYNEDVLSDAK 257 Score = 41.1 bits (92), Expect = 0.025 Identities = 23/43 (53%), Positives = 29/43 (67%), Gaps = 7/43 (16%) Frame = +2 Query: 248 GRVSGAEGFSLLRAIPGIQV-------SNXRQRARSLSSVPDL 355 G + +GF+LLR +PG+QV S RQRARSLSSVPD+ Sbjct: 56 GNDTTGQGFNLLRTLPGLQVYHNQNSTSIDRQRARSLSSVPDI 98 Score = 32.7 bits (71), Expect = 8.6 Identities = 17/25 (68%), Positives = 20/25 (80%), Gaps = 2/25 (8%) Frame = +3 Query: 174 MGAKAST--ANGGQSPRARTFSTSS 242 MG KAST +G QSPR+RTFS+SS Sbjct: 1 MGQKASTPATSGQQSPRSRTFSSSS 25 >UniRef50_UPI00015B5DB3 Cluster: PREDICTED: similar to CG14435-PA; n=1; Nasonia vitripennis|Rep: PREDICTED: similar to CG14435-PA - Nasonia vitripennis Length = 268 Score = 103 bits (246), Expect = 5e-21 Identities = 42/49 (85%), Positives = 47/49 (95%) Frame = +1 Query: 511 LPSHIWSINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLSDSK 657 LPSHIWS+NG+KCPV SKF+LPDDIECHLVMCLT+PRLSYNEDVL+D K Sbjct: 174 LPSHIWSLNGLKCPVCSKFILPDDIECHLVMCLTKPRLSYNEDVLADEK 222 >UniRef50_Q8NHG8 Cluster: E3 ubiquitin-protein ligase ZNRF2; n=15; Euteleostomi|Rep: E3 ubiquitin-protein ligase ZNRF2 - Homo sapiens (Human) Length = 242 Score = 70.5 bits (165), Expect = 4e-11 Identities = 35/62 (56%), Positives = 43/62 (69%), Gaps = 2/62 (3%) Frame = +1 Query: 469 GGVAGSGPCLRSXPLPSHI--WSINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDV 642 GG G GP L LP+H+ G KCPV SKFV D+++ HLVMCLT+PR++YNEDV Sbjct: 133 GGSPG-GPRLVIGSLPAHLSPHMFGGFKCPVCSKFVSSDEMDLHLVMCLTKPRITYNEDV 191 Query: 643 LS 648 LS Sbjct: 192 LS 193 >UniRef50_A7S6Y6 Cluster: Predicted protein; n=2; Eumetazoa|Rep: Predicted protein - Nematostella vectensis Length = 85 Score = 69.3 bits (162), Expect = 8e-11 Identities = 29/40 (72%), Positives = 34/40 (85%) Frame = +1 Query: 538 GIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLSDSK 657 G+KCPV +K VLP DIE HLV+CLT+PRLSYNEDVL + K Sbjct: 1 GLKCPVCAKVVLPQDIELHLVICLTKPRLSYNEDVLQEDK 40 >UniRef50_Q08CN9 Cluster: E3 ubiquitin-protein ligase ZNRF2; n=5; Euteleostomi|Rep: E3 ubiquitin-protein ligase ZNRF2 - Danio rerio (Zebrafish) (Brachydanio rerio) Length = 217 Score = 65.3 bits (152), Expect = 1e-09 Identities = 30/55 (54%), Positives = 39/55 (70%), Gaps = 2/55 (3%) Frame = +1 Query: 490 PCLRSXPLPSHIWS--INGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648 P L LP+H+ G KCPV SKF+ D+++ HLVMCLT+PR++YNEDVLS Sbjct: 114 PRLVIGSLPAHLSPHLFGGFKCPVCSKFISSDEMDLHLVMCLTKPRVTYNEDVLS 168 >UniRef50_UPI000155E0E6 Cluster: PREDICTED: hypothetical protein; n=1; Equus caballus|Rep: PREDICTED: hypothetical protein - Equus caballus Length = 214 Score = 63.7 bits (148), Expect = 4e-09 Identities = 26/38 (68%), Positives = 33/38 (86%) Frame = +1 Query: 535 NGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648 +G KCPV SKFV D+++ HLVMCLT+PR++YNEDVLS Sbjct: 128 SGFKCPVCSKFVPSDEMDLHLVMCLTKPRITYNEDVLS 165 >UniRef50_Q8ND25 Cluster: E3 ubiquitin-protein ligase ZNRF1; n=14; Euteleostomi|Rep: E3 ubiquitin-protein ligase ZNRF1 - Homo sapiens (Human) Length = 227 Score = 60.1 bits (139), Expect = 5e-08 Identities = 28/51 (54%), Positives = 37/51 (72%), Gaps = 5/51 (9%) Frame = +1 Query: 511 LPSHI---W--SINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648 LP HI W S +G KCP+ SK V D++E H +MCL++PRLSYN+DVL+ Sbjct: 128 LPLHIAPRWFSSHSGFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLT 178 >UniRef50_UPI0000E47BD5 Cluster: PREDICTED: hypothetical protein; n=1; Strongylocentrotus purpuratus|Rep: PREDICTED: hypothetical protein - Strongylocentrotus purpuratus Length = 213 Score = 58.8 bits (136), Expect = 1e-07 Identities = 32/60 (53%), Positives = 38/60 (63%) Frame = +1 Query: 469 GGVAGSGPCLRSXPLPSHIWSINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648 GG +GSG S S IKCPV SKFV D+IE HL+ CLT+PR+ YNEDVL+ Sbjct: 106 GGGSGSGSST-SGASGSGGGGGRSIKCPVCSKFVGADNIELHLLACLTKPRIVYNEDVLT 164 >UniRef50_Q9N4I6 Cluster: Putative uncharacterized protein; n=2; Caenorhabditis|Rep: Putative uncharacterized protein - Caenorhabditis elegans Length = 189 Score = 58.8 bits (136), Expect = 1e-07 Identities = 24/39 (61%), Positives = 30/39 (76%) Frame = +1 Query: 541 IKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLSDSK 657 +KCPV K V DD + HLVMCLTRP+++YN+DVL D K Sbjct: 104 MKCPVCHKVVPSDDADIHLVMCLTRPKITYNDDVLKDDK 142 >UniRef50_A7E2J2 Cluster: Putative uncharacterized protein; n=3; Euteleostomi|Rep: Putative uncharacterized protein - Danio rerio (Zebrafish) (Brachydanio rerio) Length = 181 Score = 58.0 bits (134), Expect = 2e-07 Identities = 25/46 (54%), Positives = 33/46 (71%) Frame = +1 Query: 511 LPSHIWSINGIKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648 L H+ + G CPV SKFV D+I+ HL+MC ++PRL YN+DVLS Sbjct: 88 LTPHLLGV-GFDCPVCSKFVCSDEIDVHLLMCFSKPRLHYNDDVLS 132 >UniRef50_Q4SIQ9 Cluster: Chromosome 21 SCAF14577, whole genome shotgun sequence; n=2; Tetraodontidae|Rep: Chromosome 21 SCAF14577, whole genome shotgun sequence - Tetraodon nigroviridis (Green puffer) Length = 205 Score = 46.0 bits (104), Expect = 9e-04 Identities = 17/34 (50%), Positives = 27/34 (79%) Frame = +1 Query: 547 CPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648 CP+ +KF+ D+IE HL+ C ++ RL+YN+D+LS Sbjct: 123 CPLCAKFMATDEIEKHLLKCFSKMRLTYNKDILS 156 >UniRef50_Q6PFI9 Cluster: Zgc:66427; n=3; Clupeocephala|Rep: Zgc:66427 - Danio rerio (Zebrafish) (Brachydanio rerio) Length = 254 Score = 37.5 bits (83), Expect = 0.30 Identities = 18/36 (50%), Positives = 22/36 (61%) Frame = +1 Query: 541 IKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS 648 I+CP +K IE HL+ CLT P L YN DVL+ Sbjct: 170 IRCPFCTKPFPGGRIEDHLLSCLTSPPLPYNTDVLA 205 >UniRef50_UPI0000DB6D3A Cluster: PREDICTED: similar to T19B10.6; n=1; Apis mellifera|Rep: PREDICTED: similar to T19B10.6 - Apis mellifera Length = 472 Score = 34.7 bits (76), Expect = 2.1 Identities = 16/51 (31%), Positives = 27/51 (52%), Gaps = 2/51 (3%) Frame = +1 Query: 541 IKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLSDSKRRMCN--MPRGV 687 +KCP+ + F+L D I H+ + NE ++S S +CN +P G+ Sbjct: 354 VKCPICNNFILNDKINEHIDFYDIESNRNVNESIISISSTSICNELIPIGI 404 >UniRef50_UPI0000D57429 Cluster: PREDICTED: similar to membrane-associated ring finger (C3HC4) 3; n=1; Tribolium castaneum|Rep: PREDICTED: similar to membrane-associated ring finger (C3HC4) 3 - Tribolium castaneum Length = 255 Score = 34.7 bits (76), Expect = 2.1 Identities = 23/70 (32%), Positives = 36/70 (51%) Frame = -2 Query: 233 RECPCARTLPPVRSARLCTHNDSVTQIANMSCTETEDHENSRDSADAPAERIVRSTETIA 54 REC R +PP +A +C H S + AN + T TED+E++R D+ E+ E Sbjct: 181 REC-VVRYIPP-STAHICCH--SPLETANPNNTPTEDNEDARSRNDSEVEQNNEEVEQGG 236 Query: 53 RLIKLALEIP 24 + +E+P Sbjct: 237 SEPTVVIEMP 246 >UniRef50_Q4RYQ4 Cluster: Chromosome 16 SCAF14974, whole genome shotgun sequence; n=2; Tetraodontidae|Rep: Chromosome 16 SCAF14974, whole genome shotgun sequence - Tetraodon nigroviridis (Green puffer) Length = 282 Score = 34.7 bits (76), Expect = 2.1 Identities = 18/47 (38%), Positives = 28/47 (59%) Frame = -3 Query: 301 NPWDGSQQAKAFRARHTSDPELVENVLARGLCPPFAVLAFAPITTPL 161 +P D QQ + A +++ P L +V + LCP +A+L +AP TPL Sbjct: 85 SPADCGQQDGSAAAAYSAAPALAADVYMQTLCPSYAMLTYAP--TPL 129 >UniRef50_Q0UXD0 Cluster: Putative uncharacterized protein; n=1; Phaeosphaeria nodorum|Rep: Putative uncharacterized protein - Phaeosphaeria nodorum (Septoria nodorum) Length = 1186 Score = 34.3 bits (75), Expect = 2.8 Identities = 28/85 (32%), Positives = 37/85 (43%), Gaps = 1/85 (1%) Frame = -2 Query: 488 PEPATPPXRHQFPHLN*NLCRXXQLQKPHSSASSGVWLLLREKRAGPVLKTETERAAXHS 309 P PA P HQ HL + R + +P + S+ L+L + AGP + E + Sbjct: 338 PRPAQRPLFHQ--HLPYPVARVFDVARPANDDSAQAPLVLLPQPAGPKVVEEKANQVWLN 395 Query: 308 TLESLGWLATS-*SLPRPTHVRPGA 237 T ES W A S S P T P A Sbjct: 396 TTESGAWYALSEISYPAVTDGAPTA 420 >UniRef50_UPI00015B4FA7 Cluster: PREDICTED: hypothetical protein; n=1; Nasonia vitripennis|Rep: PREDICTED: hypothetical protein - Nasonia vitripennis Length = 668 Score = 33.1 bits (72), Expect = 6.5 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 2/47 (4%) Frame = +1 Query: 541 IKCPVXSKFVLPDDIECHLVMCLTRPRLSYNEDVLS--DSKRRMCNM 675 I CP+ SK +LP + HL CL+ L +DV+S DS N+ Sbjct: 534 IDCPMCSKKLLPVNFNDHLQKCLSSDMLEEEDDVVSLHDSSSSSINL 580 >UniRef50_UPI0000EB2565 Cluster: UPI0000EB2565 related cluster; n=1; Canis lupus familiaris|Rep: UPI0000EB2565 UniRef100 entry - Canis familiaris Length = 526 Score = 33.1 bits (72), Expect = 6.5 Identities = 25/74 (33%), Positives = 31/74 (41%), Gaps = 1/74 (1%) Frame = -2 Query: 326 RAAXHSTLESLGWLATS*SLPRPTHVRP-GASRECPCARTLPPVRSARLCTHNDSVTQIA 150 R A H+ L + G T+ P P G+SR P AR L ARL TH S Sbjct: 102 RTAPHARLPTHGSSRTAPPHGSPRTAPPLGSSRTAPHARLLRTAPHARLLTHGSSARLPT 161 Query: 149 NMSCTETEDHENSR 108 + S H +SR Sbjct: 162 HGSSARLPTHGSSR 175 Database: uniref50 Posted date: Oct 5, 2007 11:19 AM Number of letters in database: 575,637,011 Number of sequences in database: 1,657,284 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 629,511,040 Number of Sequences: 1657284 Number of extensions: 11924288 Number of successful extensions: 35806 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 34367 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 35788 length of database: 575,637,011 effective HSP length: 98 effective length of database: 413,223,179 effective search space used: 53719013270 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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