Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30261
(688 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 113 1e-25
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 34 0.10
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 34 0.10
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 33 0.13
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 33 0.13
At3g09710.1 68416.m01150 calmodulin-binding family protein low s... 30 1.3
At5g40740.1 68418.m04944 expressed protein 28 6.7
At5g13340.1 68418.m01535 expressed protein 27 8.8
At3g56600.1 68416.m06294 phosphatidylinositol 3- and 4-kinase fa... 27 8.8
At1g79060.1 68414.m09218 expressed protein 27 8.8
At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to ... 27 8.8
At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to ... 27 8.8
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 113 bits (271), Expect = 1e-25
Identities = 53/89 (59%), Positives = 67/89 (75%)
Frame = +3
Query: 240 PENCQVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFC 419
P +VG+IQ+SIA+PT P ++Q + IF+K+K IID AG GVNI+C QE W MPFAFC
Sbjct: 87 PRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTMPFAFC 146
Query: 420 TREKQPWCEFAESAEDGPTTTFLRELAIK 506
TRE++ WCEFAE DG +T FL+ELA K
Sbjct: 147 TRERR-WCEFAEPV-DGESTKFLQELAKK 173
Score = 108 bits (260), Expect = 3e-24
Identities = 46/59 (77%), Positives = 52/59 (88%)
Frame = +2
Query: 512 MVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGH 688
MVIVS ILERD H ++LWNTAV+I + GN+IGKHRKNHIPRVGDFNES YYMEG+TGH
Sbjct: 176 MVIVSPILERDIDHGEVLWNTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGH 234
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 33.9 bits (74), Expect = 0.10
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = +2
Query: 566 WNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTG 685
+N+ +I G +G +RK+HIP + E Y+ G+TG
Sbjct: 131 YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTG 170
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 33.9 bits (74), Expect = 0.10
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = +2
Query: 566 WNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTG 685
+N+ +I G +G +RK+HIP + E Y+ G+TG
Sbjct: 104 YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTG 143
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 33.5 bits (73), Expect = 0.13
Identities = 19/69 (27%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = +3
Query: 300 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 473
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 474 TTTFLRELA 500
+T L E++
Sbjct: 154 STAMLSEVS 162
Score = 28.7 bits (61), Expect = 3.8
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = +2
Query: 545 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNT 682
E+ D L+NT V G + KHRK H+ + D +ME T
Sbjct: 176 ERVGDRLYNTCCVFGSDGELKAKHRKIHLFDI-DIPGKITFMESKT 220
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 33.5 bits (73), Expect = 0.13
Identities = 19/69 (27%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = +3
Query: 300 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 473
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 474 TTTFLRELA 500
+T L E++
Sbjct: 154 STAMLSEVS 162
Score = 28.7 bits (61), Expect = 3.8
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = +2
Query: 545 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNT 682
E+ D L+NT V G + KHRK H+ + D +ME T
Sbjct: 176 ERVGDRLYNTCCVFGSDGELKAKHRKIHLFDI-DIPGKITFMESKT 220
>At3g09710.1 68416.m01150 calmodulin-binding family protein low
similarity to SF16 protein [Helianthus annuus]
GI:560150; contains Pfam profile PF00612: IQ
calmodulin-binding motif
Length = 454
Score = 30.3 bits (65), Expect = 1.3
Identities = 19/66 (28%), Positives = 34/66 (51%), Gaps = 1/66 (1%)
Frame = +2
Query: 101 KSSTEFILGGGITLKSN*KNPRSLQLRRLTSTSPHTLSR-PRTSRPDPRELSSRNNSAFH 277
K+ST GG T KS+ +N + + T ++ T +R PR RP P + S+++
Sbjct: 290 KNSTNRNSQGGETAKSSNRNKLNSSTKPNTPSASSTATRNPRKKRPIPSSIKSKSSDDEA 349
Query: 278 RGAHRS 295
+ + R+
Sbjct: 350 KSSERN 355
>At5g40740.1 68418.m04944 expressed protein
Length = 741
Score = 27.9 bits (59), Expect = 6.7
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +2
Query: 536 ERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESN 661
ER+ + ++ +++ NVIG N PRVG F SN
Sbjct: 8 ERELELESAMYTNCLLLGLDPNVIGLGASNGTPRVGLFRHSN 49
>At5g13340.1 68418.m01535 expressed protein
Length = 242
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/50 (30%), Positives = 24/50 (48%)
Frame = +2
Query: 167 SLQLRRLTSTSPHTLSRPRTSRPDPRELSSRNNSAFHRGAHRSSSQRAKE 316
S + RR S SP+T ++ P PR+ +S+ HR + + KE
Sbjct: 27 SRRTRRDRSRSPYTSRHKKSRSPAPRQHQRDRSSSLSPSEHRIAIEVKKE 76
>At3g56600.1 68416.m06294 phosphatidylinositol 3- and 4-kinase
family protein low similarity to 55 kDa type II
phosphatidylinositol 4-kinase [Rattus norvegicus]
GI:13660755; contains Pfam profile PF00454:
Phosphatidylinositol 3- and 4-kinase
Length = 533
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/27 (51%), Positives = 17/27 (62%)
Frame = -1
Query: 559 VRMLFVPF*YRGHDHHCYLMASSRRKV 479
VR+ VPF RG DH Y +AS +R V
Sbjct: 187 VRISHVPFHDRGSDHAAYKVASLQRFV 213
>At1g79060.1 68414.m09218 expressed protein
Length = 396
Score = 27.5 bits (58), Expect = 8.8
Identities = 21/68 (30%), Positives = 29/68 (42%), Gaps = 7/68 (10%)
Frame = +2
Query: 119 ILGGGITLKSN*KNPRS-------LQLRRLTSTSPHTLSRPRTSRPDPRELSSRNNSAFH 277
I GGG + K PR L L+R + S + S T+ +PR +S H
Sbjct: 104 ISGGGDNCSFSPKAPRCSSRWRDLLGLKRFSQNSSKSASTATTTTTNPRSSTSSLKQFLH 163
Query: 278 RGAHRSSS 301
R + SSS
Sbjct: 164 RSSRSSSS 171
>At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 383
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +2
Query: 584 ISDTGNVIGKHRKNHIP 634
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
>At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 250
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +2
Query: 584 ISDTGNVIGKHRKNHIP 634
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,096,176
Number of Sequences: 28952
Number of extensions: 311034
Number of successful extensions: 987
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 966
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 986
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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