Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30255
(466 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 62 3e-11
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 49 3e-07
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 36 0.004
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 29 0.46
SPCPB1C11.01 |amt1||ammonium transporter Amt1|Schizosaccharomyce... 28 0.81
SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual 26 3.3
SPBC1683.12 |||nicotinic acid plasma membrane transporter |Schiz... 26 3.3
SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c... 25 4.3
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 25 4.3
SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIII... 25 5.7
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 25 7.5
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 24 9.9
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 62.5 bits (145), Expect = 3e-11
Identities = 22/64 (34%), Positives = 37/64 (57%)
Frame = +3
Query: 33 YCSDCYADKFATRCKGCGNPIVDKAIIALDAKWHRDCFTCMKCRNPVTDSTFSVMENQPL 212
+C CY +K+A +CK C PI+ ++ D ++H C+TC C + D + ++EN P+
Sbjct: 365 WCQTCYDNKYAVKCKKCRKPILGISVKGSDGEYHSQCWTCGACNALLGDEGYFMIENTPI 424
Query: 213 CGKC 224
C C
Sbjct: 425 CRPC 428
Score = 40.3 bits (90), Expect = 1e-04
Identities = 18/52 (34%), Positives = 28/52 (53%)
Frame = +3
Query: 6 GFMEQAGRPYCSDCYADKFATRCKGCGNPIVDKAIIALDAKWHRDCFTCMKC 161
GF + G+ YC Y ++F+ RCK C PI D+A+ + +H + C C
Sbjct: 296 GFYYREGKFYCHLDYHEQFSPRCKHCKTPIEDQAVHINNDWFHENHHFCAGC 347
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 49.2 bits (112), Expect = 3e-07
Identities = 24/67 (35%), Positives = 30/67 (44%)
Frame = +3
Query: 24 GRPYCSDCYADKFATRCKGCGNPIVDKAIIALDAKWHRDCFTCMKCRNPVTDSTFSVMEN 203
GRP CS C A C C I D A+++ +HR+CF C CR + DS F
Sbjct: 67 GRPVCSSC-----AHTCTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDNR 121
Query: 204 QPLCGKC 224
C C
Sbjct: 122 TIFCNDC 128
Score = 35.5 bits (78), Expect = 0.004
Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 5/64 (7%)
Frame = +3
Query: 51 ADKFATRCKGCGNPIVDKAI-IALDAK-WHRDCFTCMKCRNPVTDS---TFSVMENQPLC 215
+ K +T C CG + I+ WH+DCF C KC + S + +P+C
Sbjct: 12 SSKGSTVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVC 71
Query: 216 GKCA 227
CA
Sbjct: 72 SSCA 75
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 35.5 bits (78), Expect = 0.004
Identities = 18/57 (31%), Positives = 25/57 (43%), Gaps = 4/57 (7%)
Frame = +3
Query: 9 FMEQAGRPYCSDCYADKFATRCKGCGNPIVDKAI----IALDAKWHRDCFTCMKCRN 167
+ E G+ YC Y+ FA RC GC PI+ + + + WH C K N
Sbjct: 219 YYEYEGKVYCHYHYSTLFAARCCGCDGPILRQFVEVYRNGVSQNWHVPCHMIYKFWN 275
Score = 33.1 bits (72), Expect = 0.021
Identities = 15/44 (34%), Positives = 20/44 (45%)
Frame = +3
Query: 30 PYCSDCYADKFATRCKGCGNPIVDKAIIALDAKWHRDCFTCMKC 161
P C Y + C CG + I AL+ K+H + FTC C
Sbjct: 166 PLCETDYFRRLDLLCASCGMALRGYYITALNKKFHIEHFTCSLC 209
Score = 32.3 bits (70), Expect = 0.037
Identities = 13/41 (31%), Positives = 19/41 (46%)
Frame = +3
Query: 72 CKGCGNPIVDKAIIALDAKWHRDCFTCMKCRNPVTDSTFSV 194
C CG I + + AL +H +CF C C + V F +
Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPI 156
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 28.7 bits (61), Expect = 0.46
Identities = 11/39 (28%), Positives = 17/39 (43%), Gaps = 1/39 (2%)
Frame = +3
Query: 72 CKGCGNPIVDKAIIALDAK-WHRDCFTCMKCRNPVTDST 185
C C + + + K WH DCF C+ C + S+
Sbjct: 24 CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSS 62
>SPCPB1C11.01 |amt1||ammonium transporter Amt1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 497
Score = 27.9 bits (59), Expect = 0.81
Identities = 14/55 (25%), Positives = 23/55 (41%)
Frame = -3
Query: 209 WLVFHHGECRIGHWIPALHAREAIAVPLSVKRDDGFIDDWISAPLAASGELVCIA 45
W V + R HW A+A +++ GF+ W + + A G + C A
Sbjct: 271 WCVIDFAKNR--HWSVVGFCEGAVAGLVAITPGSGFVPPWAAVVIGALGAVFCYA 323
>SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual
Length = 566
Score = 25.8 bits (54), Expect = 3.3
Identities = 15/47 (31%), Positives = 22/47 (46%)
Frame = -3
Query: 236 DELSAFTAEWLVFHHGECRIGHWIPALHAREAIAVPLSVKRDDGFID 96
+E A A +F +G R+G + P + VP V RD+ F D
Sbjct: 72 NEKMANEAGGKIFTYGSYRLGVYGPGSDIDTLVVVPKHVSRDNFFQD 118
>SPBC1683.12 |||nicotinic acid plasma membrane transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 482
Score = 25.8 bits (54), Expect = 3.3
Identities = 15/61 (24%), Positives = 28/61 (45%), Gaps = 1/61 (1%)
Frame = -1
Query: 280 SVTIHLDGGF-IYIFVMN*AHLPQSGWFSITENVESVTGFLHFMHVKQSRCHLASSAMMA 104
SV ++ GG +Y+ + GWF I N + GF+ + S + ++ ++A
Sbjct: 313 SVPVYALGGISVYVICLLSDRTNIRGWFIIGMNFFGLAGFIILLATTNSAANYVATYLIA 372
Query: 103 L 101
L
Sbjct: 373 L 373
>SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 339
Score = 25.4 bits (53), Expect = 4.3
Identities = 9/22 (40%), Positives = 13/22 (59%)
Frame = +1
Query: 196 WKTSHSAVNALNSSQIYK*NPH 261
W+ + AV +N + IYK N H
Sbjct: 181 WREQYIAVGCMNDAYIYKQNSH 202
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 25.4 bits (53), Expect = 4.3
Identities = 7/20 (35%), Positives = 11/20 (55%)
Frame = -3
Query: 260 WGFYLYICDELSAFTAEWLV 201
WG+++Y C + WLV
Sbjct: 462 WGYHIYACAVIGLLDPSWLV 481
>SPAC144.09c |sfc2||RNA polymerase III transcription factor
TFIIIA|Schizosaccharomyces pombe|chr 1|||Manual
Length = 374
Score = 25.0 bits (52), Expect = 5.7
Identities = 9/21 (42%), Positives = 11/21 (52%)
Frame = -1
Query: 319 HLNSYNLCHINSKSVTIHLDG 257
HL + CH N K + H DG
Sbjct: 69 HLKIHKRCHTNVKPFSCHYDG 89
>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 855
Score = 24.6 bits (51), Expect = 7.5
Identities = 10/30 (33%), Positives = 13/30 (43%)
Frame = +3
Query: 147 TCMKCRNPVTDSTFSVMENQPLCGKCA*FI 236
TC C+ T + QPLC C F+
Sbjct: 634 TCTNCQTRTTPLWRRSPDGQPLCNACGLFM 663
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 24.2 bits (50), Expect = 9.9
Identities = 13/53 (24%), Positives = 28/53 (52%)
Frame = +1
Query: 10 SWNRLAVPIAPIAMQTSSPLAARGAEIQSSIKPSSRLTLSGTAIASRA*SAGI 168
S + L V +P+A +SSP+ + + + + S +T S ++ + + S+ I
Sbjct: 680 SSSSLIVSSSPVASSSSSPIPSSSSLVSTYSASLSNITHSSLSLTAMSSSSAI 732
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,797,182
Number of Sequences: 5004
Number of extensions: 33395
Number of successful extensions: 93
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 88
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 93
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 176367270
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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