Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30255
(466 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g19270.1 68414.m02397 ubiquitin interaction motif-containing ... 41 5e-04
At5g17890.1 68418.m02098 LIM domain-containing protein / disease... 40 6e-04
At4g36860.1 68417.m05226 LIM domain-containing protein low simil... 37 0.008
At5g66610.1 68418.m08396 LIM domain-containing protein contains ... 36 0.018
At3g55770.1 68416.m06197 LIM domain-containing protein similar t... 36 0.018
At2g45800.1 68415.m05696 LIM domain-containing protein similar t... 36 0.018
At2g39900.1 68415.m04904 LIM domain-containing protein similar t... 35 0.031
At5g66630.1 68418.m08398 LIM domain-containing protein contains ... 33 0.13
At3g61230.1 68416.m06852 LIM domain-containing protein similar t... 32 0.22
At1g01780.1 68414.m00097 LIM domain-containing protein similar t... 30 0.67
At5g60250.1 68418.m07552 zinc finger (C3HC4-type RING finger) fa... 29 1.2
At5g66620.1 68418.m08397 LIM domain-containing protein contains ... 29 1.5
At5g27150.1 68418.m03240 sodium proton exchanger / Na+/H+ antipo... 28 2.7
At2g07630.1 68415.m00881 hypothetical protein 28 2.7
At1g10200.1 68414.m01150 transcription factor LIM, putative stro... 28 2.7
At4g18780.1 68417.m02774 cellulose synthase, catalytic subunit (... 27 4.7
At3g26250.1 68416.m03275 DC1 domain-containing protein contains ... 27 4.7
At3g26240.1 68416.m03274 DC1 domain-containing protein contains ... 27 6.2
At3g42980.1 68416.m04514 hypothetical protein 27 8.3
>At1g19270.1 68414.m02397 ubiquitin interaction motif-containing
protein / LIM domain-containing protein weak similarity
to LIM-homeobox protein [Mus musculus] GI:2149584, Hic-5
[Mus musculus] GI:664955; contains Pfam profiles
PF02809: Ubiquitin interaction motif, PF00412: LIM
domain
Length = 532
Score = 40.7 bits (91), Expect = 5e-04
Identities = 17/52 (32%), Positives = 24/52 (46%), Gaps = 1/52 (1%)
Frame = +3
Query: 72 CKGCGNPIVDKAII-ALDAKWHRDCFTCMKCRNPVTDSTFSVMENQPLCGKC 224
C GC I + L++ WH +CF C C P+++ FS N P C
Sbjct: 172 CAGCNMEIGHGRFLNCLNSLWHPECFRCYGCSQPISEYEFSTSGNYPFHKAC 223
>At5g17890.1 68418.m02098 LIM domain-containing protein / disease
resistance protein-related low similarity to disease
resistance protein RPP4 [Arabidopsis thaliana]
GI:20270890; contains Pfam profiles PF00412: LIM domain,
PF00931: NB-ARC domain, PF00560: Leucine Rich Repeat
Length = 1613
Score = 40.3 bits (90), Expect = 6e-04
Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
Frame = +3
Query: 66 TRCKGCGNPIVDK-AIIALDAKWHRDCFTCMKCRNPVTDSTFS 191
++CK C + I D +I A + WH CF C++CR P+ + S
Sbjct: 1238 SKCKDCKSAIEDGISINAYGSVWHPQCFCCLRCREPIAMNEIS 1280
>At4g36860.1 68417.m05226 LIM domain-containing protein low
similarity to LIM-domain protein [Branchiostoma
floridae] GI:3360516, DRAL/Slim3/FHL2 [Homo sapiens]
GI:7209525; contains Pfam profile PF00412: LIM domain
Length = 351
Score = 36.7 bits (81), Expect = 0.008
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +3
Query: 129 WHRDCFTCMKCRNPVTDSTFSVMENQPLCGKC 224
WH +CF C C P+ D FS+ N+P C
Sbjct: 5 WHPECFCCNACDKPIIDYEFSMSGNRPYHKLC 36
>At5g66610.1 68418.m08396 LIM domain-containing protein contains
Pfam profile PF00412: LIM domain
Length = 529
Score = 35.5 bits (78), Expect = 0.018
Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 72 CKGCGNPI-VDKAIIALDAKWHRDCFTCMKCRNPV 173
C GC + I +++ AL WH +CF C C P+
Sbjct: 201 CDGCKSAIEYGRSVHALGVNWHPECFCCRYCDKPI 235
>At3g55770.1 68416.m06197 LIM domain-containing protein similar to
pollen specific LIM domain protein 1b [Nicotiana
tabacum] GI:6467905, PGPS/D1 [Petunia x hybrida]
GI:4105772; contains Pfam profile PF00412: LIM domain
Length = 199
Score = 35.5 bits (78), Expect = 0.018
Identities = 14/50 (28%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Frame = +3
Query: 69 RCKGCGNPIVDKAIIALDAK-WHRDCFTCMKCRNPVTDSTFSVMENQPLC 215
+CK C + +++ D +H+ CF C C++ + S++S ME C
Sbjct: 9 KCKACEKTVYAVELLSADGVGYHKSCFKCTHCKSRLQLSSYSSMEGVLYC 58
Score = 27.9 bits (59), Expect = 3.6
Identities = 10/50 (20%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Frame = +3
Query: 69 RCKGCGNPIVDKAIIALDAK-WHRDCFTCMKCRNPVTDSTFSVMENQPLC 215
+C C + + ++++ +H+ CF C P++ S ++ +E C
Sbjct: 107 KCATCSKTVYPIEKVTVESQTYHKSCFKCSHGGCPISPSNYAALEGILYC 156
>At2g45800.1 68415.m05696 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 226
Score = 35.5 bits (78), Expect = 0.018
Identities = 13/50 (26%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Frame = +3
Query: 69 RCKGCGNPIVDKAIIALDAK-WHRDCFTCMKCRNPVTDSTFSVMENQPLC 215
+CK C + ++ L+ +H+ CF C C+ + S +S M+ C
Sbjct: 9 KCKACDKTVYVMDLLTLEGNTYHKSCFRCTHCKGTLVISNYSSMDGVLYC 58
Score = 26.6 bits (56), Expect = 8.3
Identities = 10/50 (20%), Positives = 23/50 (46%), Gaps = 1/50 (2%)
Frame = +3
Query: 69 RCKGCGNPIVDKAIIALDAK-WHRDCFTCMKCRNPVTDSTFSVMENQPLC 215
+C C + + ++ + +H+ CF C P+T S+++ + C
Sbjct: 105 KCATCKKTVYPLEKVTMEGESYHKTCFRCTHSGCPLTHSSYASLNGVLYC 154
>At2g39900.1 68415.m04904 LIM domain-containing protein similar to
pollen specific LIM domain protein 1b [Nicotiana
tabacum] GI:6467905, PGPS/D1 [Petunia x hybrida]
GI:4105772; contains Pfam profile PF00412: LIM domain
Length = 200
Score = 34.7 bits (76), Expect = 0.031
Identities = 13/50 (26%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +3
Query: 69 RCKGCGNPIVDKAIIALDA-KWHRDCFTCMKCRNPVTDSTFSVMENQPLC 215
+C+ C + +++ D +H+ CF C C++ + S +S ME C
Sbjct: 9 KCRACEKTVYPVELLSADGISYHKACFKCSHCKSRLQLSNYSSMEGVVYC 58
Score = 27.1 bits (57), Expect = 6.2
Identities = 10/50 (20%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Frame = +3
Query: 69 RCKGCGNPIVDKAIIALDAK-WHRDCFTCMKCRNPVTDSTFSVMENQPLC 215
+C C + + ++++ +H+ CF C P++ S ++ +E C
Sbjct: 108 KCATCTKTVYPIEKVTVESQCYHKSCFKCSHGGCPISPSNYAALEGILYC 157
>At5g66630.1 68418.m08398 LIM domain-containing protein contains low
similarity to Pfam profile PF00412: LIM domain
Length = 702
Score = 32.7 bits (71), Expect = 0.13
Identities = 11/35 (31%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +3
Query: 72 CKGCGNPIV-DKAIIALDAKWHRDCFTCMKCRNPV 173
C GC + + ++++ L WH CF C C P+
Sbjct: 347 CGGCNSAVKHEESVNILGVLWHPGCFCCRSCDKPI 381
>At3g61230.1 68416.m06852 LIM domain-containing protein similar to
SP|P29675 Pollen specific protein SF3 {Helianthus
annuus}; contains Pfam profile PF00412: LIM domain
Length = 213
Score = 31.9 bits (69), Expect = 0.22
Identities = 12/50 (24%), Positives = 22/50 (44%), Gaps = 1/50 (2%)
Frame = +3
Query: 69 RCKGCGNPIVDKAIIALDAK-WHRDCFTCMKCRNPVTDSTFSVMENQPLC 215
+CK C + ++ L+ +H+ CF C C + +S M+ C
Sbjct: 10 KCKACDKTVYVMDLMTLEGMPYHKSCFRCSHCNGTLVICNYSSMDGVLYC 59
Score = 28.3 bits (60), Expect = 2.7
Identities = 10/50 (20%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Frame = +3
Query: 69 RCKGCGNPIVDKAIIALDAK-WHRDCFTCMKCRNPVTDSTFSVMENQPLC 215
+C C + + ++ + +H+ CF C P+T S+++ ++ C
Sbjct: 106 KCAACKKTVYPLEKMTMEGESYHKTCFRCAHSGCPLTHSSYAALDGVLYC 155
>At1g01780.1 68414.m00097 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 205
Score = 30.3 bits (65), Expect = 0.67
Identities = 11/50 (22%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Frame = +3
Query: 69 RCKGCGNPIVDKAIIALDAK-WHRDCFTCMKCRNPVTDSTFSVMENQPLC 215
+C C + +++++ +H+ CF C C+ + S +S M+ C
Sbjct: 9 KCNVCDKTVYVVDMLSIEGMPYHKSCFRCTHCKGTLQMSNYSSMDGVLYC 58
>At5g60250.1 68418.m07552 zinc finger (C3HC4-type RING finger)
family protein contains a Prosite:PS00518 Zinc finger,
C3HC4 type (RING finger), signature and Pfam domain
PF01485: IBR domain
Length = 655
Score = 29.5 bits (63), Expect = 1.2
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3
Query: 72 CKGCGNPIVDKAIIALDAKWHRDCFTCMK 158
C C N IV + + ++D HR CF C+K
Sbjct: 301 CAICFNDIVAEGMFSVDKCRHRFCFQCVK 329
>At5g66620.1 68418.m08397 LIM domain-containing protein contains
Pfam profile PF00412: LIM domain
Length = 644
Score = 29.1 bits (62), Expect = 1.5
Identities = 11/35 (31%), Positives = 14/35 (40%), Gaps = 1/35 (2%)
Frame = +3
Query: 72 CKGCGNPIVDKAII-ALDAKWHRDCFTCMKCRNPV 173
C GC + + L WH CF C C P+
Sbjct: 286 CGGCNFAVEHGGSVNILGVLWHPGCFCCRACHKPI 320
>At5g27150.1 68418.m03240 sodium proton exchanger / Na+/H+
antiporter (NHX1) identical to Na+/H+ exchanger
[Arabidopsis thaliana] gi|6650177|gb|AAF21755 and sodium
proton exchanger Nhx1 [Arabidopsis thaliana]
gi|4324597|gb|AAD16946; Member of The Monovalent
Cation:Proton Antiporter (CPA1) Family, PMID:11500563
Length = 538
Score = 28.3 bits (60), Expect = 2.7
Identities = 10/29 (34%), Positives = 21/29 (72%)
Frame = -1
Query: 316 LNSYNLCHINSKSVTIHLDGGFIYIFVMN 230
+ S++L H+N ++ HL G F+Y+F+++
Sbjct: 195 IQSFDLTHLNHEAA-FHLLGNFLYLFLLS 222
>At2g07630.1 68415.m00881 hypothetical protein
Length = 458
Score = 28.3 bits (60), Expect = 2.7
Identities = 14/46 (30%), Positives = 19/46 (41%)
Frame = +3
Query: 108 IIALDAKWHRDCFTCMKCRNPVTDSTFSVMENQPLCGKCA*FITNI 245
I +D W C KC VT + ++ Q C C ITN+
Sbjct: 260 IYDIDRDWSWYYIVCKKCNKKVTKVVMTSLKAQLWCETCRAPITNV 305
>At1g10200.1 68414.m01150 transcription factor LIM, putative strong
similarity to transcription factor Ntlim1 [Nicotiana
tabacum] GI:5689136, LIM domain protein WLIM-1
[Helianthus annuus] GI:5070280; contains Pfam profile
PF00412: LIM domain
Length = 190
Score = 28.3 bits (60), Expect = 2.7
Identities = 14/51 (27%), Positives = 24/51 (47%), Gaps = 2/51 (3%)
Frame = +3
Query: 69 RCKGCGNPI--VDKAIIALDAKWHRDCFTCMKCRNPVTDSTFSVMENQPLC 215
+C C + VDK + A + +H+ CF C C+ + S ++ E C
Sbjct: 9 KCMACDKTVYLVDK-LTADNRVYHKACFRCHHCKGTLKLSNYNSFEGVLYC 58
>At4g18780.1 68417.m02774 cellulose synthase, catalytic subunit
(IRX1) nearly identical to gi:12836997
Length = 985
Score = 27.5 bits (58), Expect = 4.7
Identities = 19/72 (26%), Positives = 32/72 (44%), Gaps = 7/72 (9%)
Frame = +3
Query: 12 MEQAGRPYCSDCYAD-------KFATRCKGCGNPIVDKAIIALDAKWHRDCFTCMKCRNP 170
M ++ P C+ C + +F C C PI KA + + K R C++C NP
Sbjct: 1 MMESRSPICNTCGEEIGVKSNGEFFVACHECSFPIC-KACLEYEFKEGRRI--CLRCGNP 57
Query: 171 VTDSTFSVMENQ 206
++ F +E +
Sbjct: 58 YDENVFDDVETK 69
>At3g26250.1 68416.m03275 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 490
Score = 27.5 bits (58), Expect = 4.7
Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 3/34 (8%)
Frame = +3
Query: 135 RDCFTCMKCRNPVTDSTFSVMENQ---PLCGKCA 227
R+C C + R T TF+ +E + LC KCA
Sbjct: 308 RNCSVCAEARGYYTKETFNCIEEECDFALCFKCA 341
>At3g26240.1 68416.m03274 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 922
Score = 27.1 bits (57), Expect = 6.2
Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 3/34 (8%)
Frame = +3
Query: 135 RDCFTCMKCRNPVTDSTFSVMENQ---PLCGKCA 227
R+C C K T TF+ +E + LC KCA
Sbjct: 742 RNCSVCAKAPGEYTKETFNCIEEECDYALCFKCA 775
>At3g42980.1 68416.m04514 hypothetical protein
Length = 303
Score = 26.6 bits (56), Expect = 8.3
Identities = 17/53 (32%), Positives = 24/53 (45%), Gaps = 7/53 (13%)
Frame = +3
Query: 108 IIALDAKWHRDCFTCMKCRNPVTD-----STFSVMENQP--LCGKCA*FITNI 245
I ALD W F+C C VT +T S+ ++P C C +TN+
Sbjct: 248 IYALDTDWSWYYFSCRNCNKKVTHIHAGVNTTSLKSSKPRFWCDVCKNGVTNV 300
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,447,144
Number of Sequences: 28952
Number of extensions: 177806
Number of successful extensions: 449
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 439
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 449
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 782033640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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