BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30230 (603 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC14C8.12 |rpb8||DNA-directed RNA polymerase I, II and III sub... 53 4e-08 SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyce... 27 2.1 SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 27 2.8 SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 26 4.9 SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosacch... 25 8.5 >SPBC14C8.12 |rpb8||DNA-directed RNA polymerase I, II and III subunit Rpb8|Schizosaccharomyces pombe|chr 2|||Manual Length = 125 Score = 52.8 bits (121), Expect = 4e-08 Identities = 28/68 (41%), Positives = 45/68 (66%) Frame = +1 Query: 304 NRRNRADSFEYVMSGKVYRIEGDEAAMEPASRLAAYVSFGGLLMRLQGDANNLHGFEVDQ 483 N + ++ +Y+M GKVYR+E EA E +++ YVSFGGLLM ++G L+ +D Sbjct: 64 NSPDLKEAADYIMYGKVYRVE--EAKDE---KVSVYVSFGGLLMAIEGSHRKLYRLSLD- 117 Query: 484 HMYLLMKK 507 H+YLL+++ Sbjct: 118 HVYLLLRR 125 Score = 44.8 bits (101), Expect = 1e-05 Identities = 25/65 (38%), Positives = 36/65 (55%) Frame = +3 Query: 78 VLFEDIFNVKDMDPEGKKFDRVSRLHCESESFKMDLILDINSWIYPMQLGEKFRLVLATR 257 VL ++IF V +D + K+ RVSR+ S M+L LDINS IYP++ F L + + Sbjct: 5 VLLDEIFTVTSVDKQ--KYQRVSRITAVSGQNDMNLTLDINSQIYPLEKDATFSLQITSN 62 Query: 258 *GKTD 272 D Sbjct: 63 LNSPD 67 >SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 973 Score = 27.1 bits (57), Expect = 2.1 Identities = 26/80 (32%), Positives = 34/80 (42%), Gaps = 2/80 (2%) Frame = -3 Query: 406 PLIW--MPAPSLPRHLLSCILFRT*HIRNYLLDFFGFQRVPFSAVRVSVFPQRVAKTRRN 233 PL W + P LPR L+SC +I L FF VP + P V +N Sbjct: 508 PLTWQGLYIPVLPRRLISCFEAPCSYIIGTLSYFFHMDDVPLDNI-----PLVVCDLDKN 562 Query: 232 FSPSCIGYIQELMSNIKSIL 173 S S G I L +++S L Sbjct: 563 -SVSTFGKIVRLSRSLRSKL 581 >SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces pombe|chr 1|||Manual Length = 949 Score = 26.6 bits (56), Expect = 2.8 Identities = 13/42 (30%), Positives = 23/42 (54%) Frame = +3 Query: 102 VKDMDPEGKKFDRVSRLHCESESFKMDLILDINSWIYPMQLG 227 ++ +D GK SRL E ++ +D+ +NS +Y + LG Sbjct: 720 LETLDDYGKLLAHFSRLAPEFQTGMLDMFRTLNSRVYQLILG 761 >SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1253 Score = 25.8 bits (54), Expect = 4.9 Identities = 12/40 (30%), Positives = 23/40 (57%) Frame = -3 Query: 166 SDSQCNRLTLSNFLPSGSMSLTLKMSSNSTPAMIKFYPVN 47 S+ Q + +L ++ PSGS+ T+K S+ + + F+ N Sbjct: 248 SEPQDGQQSLLSWKPSGSVLATIKSDSSDNSSKVIFFERN 287 >SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 533 Score = 25.0 bits (52), Expect = 8.5 Identities = 12/25 (48%), Positives = 16/25 (64%) Frame = +3 Query: 126 KKFDRVSRLHCESESFKMDLILDIN 200 KK R++RL ESE F+ + DIN Sbjct: 71 KKTVRLNRLQRESEQFENSALKDIN 95 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,455,835 Number of Sequences: 5004 Number of extensions: 52943 Number of successful extensions: 157 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 149 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 156 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 264253462 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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