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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30230
         (603 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g54250.1 68414.m06185 DNA-directed RNA polymerase I, II, and ...    70   1e-12
At3g59600.1 68416.m06650 DNA-directed RNA polymerase I, II, and ...    69   3e-12
At5g02020.1 68418.m00121 expressed protein                             30   1.0  
At5g27060.1 68418.m03229 disease resistance family protein conta...    29   2.4  
At5g57900.1 68418.m07243 SKP1/ASK1 interacting partner 1 (SKIP1)...    28   4.1  
At5g02020.2 68418.m00122 expressed protein                             28   4.1  
At4g31250.1 68417.m04436 leucine-rich repeat transmembrane prote...    28   4.1  
At1g64580.1 68414.m07320 pentatricopeptide (PPR) repeat-containi...    28   4.1  
At4g32295.1 68417.m04595 expressed protein                             28   5.5  
At1g67120.1 68414.m07636 midasin-related similar to Midasin (MID...    28   5.5  
At2g28710.1 68415.m03490 zinc finger (C2H2 type) family protein ...    27   7.2  
At1g14790.1 68414.m01768 RNA-dependent RNA polymerase, putative ...    27   9.6  

>At1g54250.1 68414.m06185 DNA-directed RNA polymerase I, II, and
           III, putative similar to SP|P52434 DNA-directed RNA
           polymerases I, II, and III 17.1 kDa polypeptide (EC
           2.7.7.6) (RPB17) (RPB8) {Homo sapiens}; contains Pfam
           profile PF03870: RNA polymerase Rpb8
          Length = 146

 Score = 70.1 bits (164), Expect = 1e-12
 Identities = 29/58 (50%), Positives = 44/58 (75%)
 Frame = +3

Query: 78  VLFEDIFNVKDMDPEGKKFDRVSRLHCESESFKMDLILDINSWIYPMQLGEKFRLVLA 251
           +LFEDIF V  +DP+GKKFD+V+R+   S + +M + LD+N+ +YP+ +G+KF L LA
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALA 63



 Score = 66.1 bits (154), Expect = 2e-11
 Identities = 32/69 (46%), Positives = 47/69 (68%)
 Frame = +1

Query: 301 GNRRNRADSFEYVMSGKVYRIEGDEAAMEPASRLAAYVSFGGLLMRLQGDANNLHGFEVD 480
           G ++  AD +EY+M GK+Y+I  +     P + L  YVSFGGLLM L+GD  ++  FE+D
Sbjct: 80  GAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAEL--YVSFGGLLMLLKGDPAHISHFELD 136

Query: 481 QHMYLLMKK 507
           Q ++LLM+K
Sbjct: 137 QRLFLLMRK 145


>At3g59600.1 68416.m06650 DNA-directed RNA polymerase I, II, and
           III, putative similar to SP|P52434 DNA-directed RNA
           polymerases I, II, and III 17.1 kDa polypeptide (EC
           2.7.7.6) (RPB17) (RPB8) {Homo sapiens}; contains Pfam
           profile PF03870: RNA polymerase Rpb8
          Length = 146

 Score = 68.5 bits (160), Expect = 3e-12
 Identities = 27/58 (46%), Positives = 44/58 (75%)
 Frame = +3

Query: 78  VLFEDIFNVKDMDPEGKKFDRVSRLHCESESFKMDLILDINSWIYPMQLGEKFRLVLA 251
           ++FEDIF V  +DP+GKKFD+V+R+   S + +M + LD+N+ +YP+ +G+KF L +A
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMA 63



 Score = 67.7 bits (158), Expect = 6e-12
 Identities = 33/69 (47%), Positives = 47/69 (68%)
 Frame = +1

Query: 301 GNRRNRADSFEYVMSGKVYRIEGDEAAMEPASRLAAYVSFGGLLMRLQGDANNLHGFEVD 480
           G ++  AD +EY+M GK+Y+I  +     P + L  YVSFGGLLM LQGD  ++  FE+D
Sbjct: 80  GAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAEL--YVSFGGLLMLLQGDPAHISHFELD 136

Query: 481 QHMYLLMKK 507
           Q ++LLM+K
Sbjct: 137 QRLFLLMRK 145


>At5g02020.1 68418.m00121 expressed protein
          Length = 149

 Score = 30.3 bits (65), Expect = 1.0
 Identities = 19/74 (25%), Positives = 32/74 (43%)
 Frame = +2

Query: 71  GGRAIRGHFQRQRHGSGGQKVRQSQPIALRIGVIQNGFNIGH*FLDISNATRRKVSSSFG 250
           GG   R   Q++ HGSG Q+ ++ QP  L   +   G ++   +    N+T    +   G
Sbjct: 73  GGNVDRNREQQENHGSGYQQDQRVQPCHLSSSIYYGGPDV---YFQPQNSTSNSTNKKDG 129

Query: 251 HALRENGYPDGGEW 292
               ++G    G W
Sbjct: 130 -GEDDSGSASRGNW 142


>At5g27060.1 68418.m03229 disease resistance family protein contains
           leucine rich-repeat (LRR) domains Pfam:PF00560,
           INTERPRO:IPR001611; similar to Hcr2-0B [Lycopersicon
           esculentum] gi|3894387|gb|AAC78593
          Length = 957

 Score = 29.1 bits (62), Expect = 2.4
 Identities = 18/45 (40%), Positives = 25/45 (55%), Gaps = 5/45 (11%)
 Frame = -2

Query: 425 PPNET*AANL---DAGSIAASS--PSILYTFPDMTYSKLSARFLR 306
           PPN T  +NL   DA   A +   PS L+T P +TY +L+   L+
Sbjct: 330 PPNITSLSNLMDFDASDNAFTGTFPSFLFTIPSLTYIRLNGNQLK 374


>At5g57900.1 68418.m07243 SKP1/ASK1 interacting partner 1 (SKIP1) /
           SCF (Skp1-cullin-F-box) ubiquitin ligase identical to
           SKP1 interacting partner 1 GI:10716947 from [Arabidopsis
           thaliana], PMID:11387208
          Length = 300

 Score = 28.3 bits (60), Expect = 4.1
 Identities = 11/36 (30%), Positives = 19/36 (52%)
 Frame = +2

Query: 278 DGGEWNPLETEEIEQIVSNMSCPEKYTG*KVTRQRW 385
           DG +W  L  E +  I+S ++  E +TG    ++ W
Sbjct: 4   DGSDWGGLAPEILINIISRLTIQELWTGPMFVQKSW 39


>At5g02020.2 68418.m00122 expressed protein
          Length = 137

 Score = 28.3 bits (60), Expect = 4.1
 Identities = 13/37 (35%), Positives = 19/37 (51%)
 Frame = +2

Query: 71  GGRAIRGHFQRQRHGSGGQKVRQSQPIALRIGVIQNG 181
           GG   R   Q++ HGSG Q+ ++ QP  L   +   G
Sbjct: 73  GGNVDRNREQQENHGSGYQQDQRVQPCHLSSSIYYGG 109


>At4g31250.1 68417.m04436 leucine-rich repeat transmembrane protein
           kinase, putative receptor kinase, Petunia inflata,
           Patchx:G498278
          Length = 676

 Score = 28.3 bits (60), Expect = 4.1
 Identities = 21/79 (26%), Positives = 32/79 (40%), Gaps = 2/79 (2%)
 Frame = +1

Query: 301 GNRRNRADSFEYVMSGKVYRIEGDEAAMEPASRLAAYVSFGGLL--MRLQGDANNLHGFE 474
           G    +  S +     KVYR   +E     ++  +  +S GGL      +GD   LH   
Sbjct: 294 GQPEQQQHSEKSSQDSKVYRKLANETVQRDSTATSGAISVGGLSPDEDKRGDQRKLHFVR 353

Query: 475 VDQHMYLLMKK*RFSSIIL 531
            DQ  + L    R S+ +L
Sbjct: 354 NDQERFTLQDMLRASAEVL 372


>At1g64580.1 68414.m07320 pentatricopeptide (PPR) repeat-containing
            protein low similarity to fertility restorer [Petunia x
            hybrida] GI:22128587; contains Pfam profile PF01535: PPR
            repeat
          Length = 1052

 Score = 28.3 bits (60), Expect = 4.1
 Identities = 14/44 (31%), Positives = 23/44 (52%), Gaps = 2/44 (4%)
 Frame = -3

Query: 244  TRRNFSPSCIGYIQELMSNI--KSILNDSDSQCNRLTLSNFLPS 119
            TR+   P  I YI  ++S +  K +  ++D  C R+    F+PS
Sbjct: 985  TRKGVKPDAIAYIT-MISGLCRKGLQREADKLCRRMKEDGFMPS 1027


>At4g32295.1 68417.m04595 expressed protein
          Length = 238

 Score = 27.9 bits (59), Expect = 5.5
 Identities = 17/80 (21%), Positives = 38/80 (47%)
 Frame = -3

Query: 286 SAVRVSVFPQRVAKTRRNFSPSCIGYIQELMSNIKSILNDSDSQCNRLTLSNFLPSGSMS 107
           +A+ +   P+R ++++RN +P C  Y+ +  +N+ ++L+    + N     N   +    
Sbjct: 111 AALHLGCTPRRASRSQRNINPRC--YLSQDSTNLDNMLSQVFMKPNNFAPKNLAVAQENC 168

Query: 106 LTLKMSSNSTPAMIKFYPVN 47
              K S  + P    F P++
Sbjct: 169 PVSKYS--AYPLCYSFRPIS 186


>At1g67120.1 68414.m07636 midasin-related similar to Midasin
            (MIDAS-containing protein) (Swiss-Prot:Q12019)
            [Saccharomyces cerevisiae]; similar to Midasin
            (MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo
            sapiens]; contains Prosite PS00017: ATP/GTP-binding site
            motif A (P-loop)
          Length = 5336

 Score = 27.9 bits (59), Expect = 5.5
 Identities = 22/68 (32%), Positives = 31/68 (45%), Gaps = 1/68 (1%)
 Frame = -3

Query: 322  LLDFFGFQRVPFSAVRVSVFPQRVAKTRRNFSPSCIGYIQELMSNIKSIL-NDSDSQCNR 146
            LLD  GF   P S+++  V    V   R+NF       + +L + I S   N+  S C  
Sbjct: 4246 LLDCNGFIITPSSSLKQFVTQHMVQVLRQNFD-----QLTDLENQISSFCENNEKSYCRD 4300

Query: 145  LTLSNFLP 122
            + LS F P
Sbjct: 4301 VLLSQFSP 4308


>At2g28710.1 68415.m03490 zinc finger (C2H2 type) family protein
           contains Pfam domain, PF00096: Zinc finger, C2H2 type
          Length = 156

 Score = 27.5 bits (58), Expect = 7.2
 Identities = 12/36 (33%), Positives = 20/36 (55%)
 Frame = +2

Query: 74  GRAIRGHFQRQRHGSGGQKVRQSQPIALRIGVIQNG 181
           G+A+ GH ++ R GSGG   R   P    + + ++G
Sbjct: 90  GQALGGHMRKHRGGSGGGGGRSLAPATAPVTMKKSG 125


>At1g14790.1 68414.m01768 RNA-dependent RNA polymerase, putative
            similar to RNA-directed RNA polymerase GB:CAA09697
            GI:4138282 [Nicotiana tabacum]
          Length = 1107

 Score = 27.1 bits (57), Expect = 9.6
 Identities = 17/68 (25%), Positives = 29/68 (42%), Gaps = 2/68 (2%)
 Frame = -2

Query: 539  FTNNIIELKRYFFINKYMCWSTSKPCKLFASPCSRIRRPPNET*AANLDAGSIAASSPSI 360
            F N I+        N +  ++  +P K F+ PC  + +  +   A +     +AA  P  
Sbjct: 843  FANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFST--AVDFPKTGVAAVIPQH 900

Query: 359  LYT--FPD 342
            LY   +PD
Sbjct: 901  LYVKEYPD 908


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,898,174
Number of Sequences: 28952
Number of extensions: 288652
Number of successful extensions: 823
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 796
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 821
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1197101088
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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