BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30230 (603 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g54250.1 68414.m06185 DNA-directed RNA polymerase I, II, and ... 70 1e-12 At3g59600.1 68416.m06650 DNA-directed RNA polymerase I, II, and ... 69 3e-12 At5g02020.1 68418.m00121 expressed protein 30 1.0 At5g27060.1 68418.m03229 disease resistance family protein conta... 29 2.4 At5g57900.1 68418.m07243 SKP1/ASK1 interacting partner 1 (SKIP1)... 28 4.1 At5g02020.2 68418.m00122 expressed protein 28 4.1 At4g31250.1 68417.m04436 leucine-rich repeat transmembrane prote... 28 4.1 At1g64580.1 68414.m07320 pentatricopeptide (PPR) repeat-containi... 28 4.1 At4g32295.1 68417.m04595 expressed protein 28 5.5 At1g67120.1 68414.m07636 midasin-related similar to Midasin (MID... 28 5.5 At2g28710.1 68415.m03490 zinc finger (C2H2 type) family protein ... 27 7.2 At1g14790.1 68414.m01768 RNA-dependent RNA polymerase, putative ... 27 9.6 >At1g54250.1 68414.m06185 DNA-directed RNA polymerase I, II, and III, putative similar to SP|P52434 DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide (EC 2.7.7.6) (RPB17) (RPB8) {Homo sapiens}; contains Pfam profile PF03870: RNA polymerase Rpb8 Length = 146 Score = 70.1 bits (164), Expect = 1e-12 Identities = 29/58 (50%), Positives = 44/58 (75%) Frame = +3 Query: 78 VLFEDIFNVKDMDPEGKKFDRVSRLHCESESFKMDLILDINSWIYPMQLGEKFRLVLA 251 +LFEDIF V +DP+GKKFD+V+R+ S + +M + LD+N+ +YP+ +G+KF L LA Sbjct: 6 ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALA 63 Score = 66.1 bits (154), Expect = 2e-11 Identities = 32/69 (46%), Positives = 47/69 (68%) Frame = +1 Query: 301 GNRRNRADSFEYVMSGKVYRIEGDEAAMEPASRLAAYVSFGGLLMRLQGDANNLHGFEVD 480 G ++ AD +EY+M GK+Y+I + P + L YVSFGGLLM L+GD ++ FE+D Sbjct: 80 GAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAEL--YVSFGGLLMLLKGDPAHISHFELD 136 Query: 481 QHMYLLMKK 507 Q ++LLM+K Sbjct: 137 QRLFLLMRK 145 >At3g59600.1 68416.m06650 DNA-directed RNA polymerase I, II, and III, putative similar to SP|P52434 DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide (EC 2.7.7.6) (RPB17) (RPB8) {Homo sapiens}; contains Pfam profile PF03870: RNA polymerase Rpb8 Length = 146 Score = 68.5 bits (160), Expect = 3e-12 Identities = 27/58 (46%), Positives = 44/58 (75%) Frame = +3 Query: 78 VLFEDIFNVKDMDPEGKKFDRVSRLHCESESFKMDLILDINSWIYPMQLGEKFRLVLA 251 ++FEDIF V +DP+GKKFD+V+R+ S + +M + LD+N+ +YP+ +G+KF L +A Sbjct: 6 IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMA 63 Score = 67.7 bits (158), Expect = 6e-12 Identities = 33/69 (47%), Positives = 47/69 (68%) Frame = +1 Query: 301 GNRRNRADSFEYVMSGKVYRIEGDEAAMEPASRLAAYVSFGGLLMRLQGDANNLHGFEVD 480 G ++ AD +EY+M GK+Y+I + P + L YVSFGGLLM LQGD ++ FE+D Sbjct: 80 GAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAEL--YVSFGGLLMLLQGDPAHISHFELD 136 Query: 481 QHMYLLMKK 507 Q ++LLM+K Sbjct: 137 QRLFLLMRK 145 >At5g02020.1 68418.m00121 expressed protein Length = 149 Score = 30.3 bits (65), Expect = 1.0 Identities = 19/74 (25%), Positives = 32/74 (43%) Frame = +2 Query: 71 GGRAIRGHFQRQRHGSGGQKVRQSQPIALRIGVIQNGFNIGH*FLDISNATRRKVSSSFG 250 GG R Q++ HGSG Q+ ++ QP L + G ++ + N+T + G Sbjct: 73 GGNVDRNREQQENHGSGYQQDQRVQPCHLSSSIYYGGPDV---YFQPQNSTSNSTNKKDG 129 Query: 251 HALRENGYPDGGEW 292 ++G G W Sbjct: 130 -GEDDSGSASRGNW 142 >At5g27060.1 68418.m03229 disease resistance family protein contains leucine rich-repeat (LRR) domains Pfam:PF00560, INTERPRO:IPR001611; similar to Hcr2-0B [Lycopersicon esculentum] gi|3894387|gb|AAC78593 Length = 957 Score = 29.1 bits (62), Expect = 2.4 Identities = 18/45 (40%), Positives = 25/45 (55%), Gaps = 5/45 (11%) Frame = -2 Query: 425 PPNET*AANL---DAGSIAASS--PSILYTFPDMTYSKLSARFLR 306 PPN T +NL DA A + PS L+T P +TY +L+ L+ Sbjct: 330 PPNITSLSNLMDFDASDNAFTGTFPSFLFTIPSLTYIRLNGNQLK 374 >At5g57900.1 68418.m07243 SKP1/ASK1 interacting partner 1 (SKIP1) / SCF (Skp1-cullin-F-box) ubiquitin ligase identical to SKP1 interacting partner 1 GI:10716947 from [Arabidopsis thaliana], PMID:11387208 Length = 300 Score = 28.3 bits (60), Expect = 4.1 Identities = 11/36 (30%), Positives = 19/36 (52%) Frame = +2 Query: 278 DGGEWNPLETEEIEQIVSNMSCPEKYTG*KVTRQRW 385 DG +W L E + I+S ++ E +TG ++ W Sbjct: 4 DGSDWGGLAPEILINIISRLTIQELWTGPMFVQKSW 39 >At5g02020.2 68418.m00122 expressed protein Length = 137 Score = 28.3 bits (60), Expect = 4.1 Identities = 13/37 (35%), Positives = 19/37 (51%) Frame = +2 Query: 71 GGRAIRGHFQRQRHGSGGQKVRQSQPIALRIGVIQNG 181 GG R Q++ HGSG Q+ ++ QP L + G Sbjct: 73 GGNVDRNREQQENHGSGYQQDQRVQPCHLSSSIYYGG 109 >At4g31250.1 68417.m04436 leucine-rich repeat transmembrane protein kinase, putative receptor kinase, Petunia inflata, Patchx:G498278 Length = 676 Score = 28.3 bits (60), Expect = 4.1 Identities = 21/79 (26%), Positives = 32/79 (40%), Gaps = 2/79 (2%) Frame = +1 Query: 301 GNRRNRADSFEYVMSGKVYRIEGDEAAMEPASRLAAYVSFGGLL--MRLQGDANNLHGFE 474 G + S + KVYR +E ++ + +S GGL +GD LH Sbjct: 294 GQPEQQQHSEKSSQDSKVYRKLANETVQRDSTATSGAISVGGLSPDEDKRGDQRKLHFVR 353 Query: 475 VDQHMYLLMKK*RFSSIIL 531 DQ + L R S+ +L Sbjct: 354 NDQERFTLQDMLRASAEVL 372 >At1g64580.1 68414.m07320 pentatricopeptide (PPR) repeat-containing protein low similarity to fertility restorer [Petunia x hybrida] GI:22128587; contains Pfam profile PF01535: PPR repeat Length = 1052 Score = 28.3 bits (60), Expect = 4.1 Identities = 14/44 (31%), Positives = 23/44 (52%), Gaps = 2/44 (4%) Frame = -3 Query: 244 TRRNFSPSCIGYIQELMSNI--KSILNDSDSQCNRLTLSNFLPS 119 TR+ P I YI ++S + K + ++D C R+ F+PS Sbjct: 985 TRKGVKPDAIAYIT-MISGLCRKGLQREADKLCRRMKEDGFMPS 1027 >At4g32295.1 68417.m04595 expressed protein Length = 238 Score = 27.9 bits (59), Expect = 5.5 Identities = 17/80 (21%), Positives = 38/80 (47%) Frame = -3 Query: 286 SAVRVSVFPQRVAKTRRNFSPSCIGYIQELMSNIKSILNDSDSQCNRLTLSNFLPSGSMS 107 +A+ + P+R ++++RN +P C Y+ + +N+ ++L+ + N N + Sbjct: 111 AALHLGCTPRRASRSQRNINPRC--YLSQDSTNLDNMLSQVFMKPNNFAPKNLAVAQENC 168 Query: 106 LTLKMSSNSTPAMIKFYPVN 47 K S + P F P++ Sbjct: 169 PVSKYS--AYPLCYSFRPIS 186 >At1g67120.1 68414.m07636 midasin-related similar to Midasin (MIDAS-containing protein) (Swiss-Prot:Q12019) [Saccharomyces cerevisiae]; similar to Midasin (MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo sapiens]; contains Prosite PS00017: ATP/GTP-binding site motif A (P-loop) Length = 5336 Score = 27.9 bits (59), Expect = 5.5 Identities = 22/68 (32%), Positives = 31/68 (45%), Gaps = 1/68 (1%) Frame = -3 Query: 322 LLDFFGFQRVPFSAVRVSVFPQRVAKTRRNFSPSCIGYIQELMSNIKSIL-NDSDSQCNR 146 LLD GF P S+++ V V R+NF + +L + I S N+ S C Sbjct: 4246 LLDCNGFIITPSSSLKQFVTQHMVQVLRQNFD-----QLTDLENQISSFCENNEKSYCRD 4300 Query: 145 LTLSNFLP 122 + LS F P Sbjct: 4301 VLLSQFSP 4308 >At2g28710.1 68415.m03490 zinc finger (C2H2 type) family protein contains Pfam domain, PF00096: Zinc finger, C2H2 type Length = 156 Score = 27.5 bits (58), Expect = 7.2 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +2 Query: 74 GRAIRGHFQRQRHGSGGQKVRQSQPIALRIGVIQNG 181 G+A+ GH ++ R GSGG R P + + ++G Sbjct: 90 GQALGGHMRKHRGGSGGGGGRSLAPATAPVTMKKSG 125 >At1g14790.1 68414.m01768 RNA-dependent RNA polymerase, putative similar to RNA-directed RNA polymerase GB:CAA09697 GI:4138282 [Nicotiana tabacum] Length = 1107 Score = 27.1 bits (57), Expect = 9.6 Identities = 17/68 (25%), Positives = 29/68 (42%), Gaps = 2/68 (2%) Frame = -2 Query: 539 FTNNIIELKRYFFINKYMCWSTSKPCKLFASPCSRIRRPPNET*AANLDAGSIAASSPSI 360 F N I+ N + ++ +P K F+ PC + + + A + +AA P Sbjct: 843 FANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFST--AVDFPKTGVAAVIPQH 900 Query: 359 LYT--FPD 342 LY +PD Sbjct: 901 LYVKEYPD 908 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,898,174 Number of Sequences: 28952 Number of extensions: 288652 Number of successful extensions: 823 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 796 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 821 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1197101088 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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