BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30215
(655 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13C5.07 |rad32|mre11|Rad32 nuclease|Schizosaccharomyces pomb... 27 1.8
SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein ... 27 3.1
SPMIT.11 |cox2||cytochrome c oxidase 2|Schizosaccharomyces pombe... 26 5.5
SPBC83.12 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 25 7.2
SPAC458.05 |pik3|vps34|phosphatidylinositol 3-kinase Pik3|Schizo... 25 7.2
SPAC1F3.07c |rsc58||RSC complex subunit Rsc58|Schizosaccharomyce... 25 7.2
SPCC306.11 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 25 9.5
SPAC23A1.12c |||phenylalanine-tRNA ligase beta subunit |Schizosa... 25 9.5
>SPAC13C5.07 |rad32|mre11|Rad32 nuclease|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 649
Score = 27.5 bits (58), Expect = 1.8
Identities = 15/49 (30%), Positives = 24/49 (48%), Gaps = 3/49 (6%)
Frame = +2
Query: 41 DYQSNLLIMSNDYGFNSDDSP---NKTNSHLQLPSPIITRRNRTASTSE 178
D+ S L S+++ + SP KTN +LPS + + RT S+
Sbjct: 552 DHHSPLATSSSEHEMEATPSPALLKKTNKRRELPSSLTKKNTRTPQRSK 600
>SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein
S0B|Schizosaccharomyces pombe|chr 1|||Manual
Length = 287
Score = 26.6 bits (56), Expect = 3.1
Identities = 19/51 (37%), Positives = 25/51 (49%), Gaps = 5/51 (9%)
Frame = +3
Query: 336 ISTLPNPT*VRKISSCPCAHN-----SSHSGETRQVGRTTSVTIKNPRYVT 473
I+T+ NP V ISS P H ++H+G T GR T N Y+T
Sbjct: 68 IATIENPADVCVISSRPYGHRAVLKFAAHTGATAIAGRFTPGNFTN--YIT 116
>SPMIT.11 |cox2||cytochrome c oxidase 2|Schizosaccharomyces
pombe|chr mitochondrial|||Manual
Length = 248
Score = 25.8 bits (54), Expect = 5.5
Identities = 25/112 (22%), Positives = 47/112 (41%), Gaps = 1/112 (0%)
Frame = -2
Query: 420 VFLRSEMNYAHMDSLKFFELRWDWAKSIYNFITRQGYIFTFDIRYMEKYIXXXXXXXXXX 241
++L E+ M ++K +W W+ + +F+T + +FD YM +
Sbjct: 100 LYLLDEVQKPSM-TVKAIGRQWFWSYELNDFVTNENEPVSFD-SYM---VPEEDLEEGSL 154
Query: 240 XXFSLQNVLILPVSNGLPKALSDVDAVRFRRV-IIGLGNCKCEFVLLGESSL 88
+ N L+LP+ + L+ D + V +G+ C C L + SL
Sbjct: 155 RQLEVDNRLVLPIDTRIRLILTSGDVIHSWAVPSLGI-KCDCIPSRLNQVSL 205
>SPBC83.12 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 161
Score = 25.4 bits (53), Expect = 7.2
Identities = 10/35 (28%), Positives = 19/35 (54%)
Frame = +2
Query: 236 GHGFVKLKKGAKIYFSIYLISKVNMYPCLVMKLYI 340
GHG++ +K +F+ L + + Y C++ YI
Sbjct: 10 GHGYILIKPNYPDHFNYRLPADMKFYKCVLTINYI 44
>SPAC458.05 |pik3|vps34|phosphatidylinositol 3-kinase
Pik3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 801
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +2
Query: 14 LTYLLPILDDYQSNLLIMSNDYGFNSD 94
+TYLL + D + NLLI + + F++D
Sbjct: 649 ITYLLGVGDRHLDNLLITKDGHFFHAD 675
>SPAC1F3.07c |rsc58||RSC complex subunit Rsc58|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 403
Score = 25.4 bits (53), Expect = 7.2
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = +2
Query: 47 QSNLLIMSNDYGFNSDDSPNKTNSHLQ 127
QSN + SN N SP+ TN LQ
Sbjct: 367 QSNAALRSNSLSMNGSLSPSSTNVPLQ 393
>SPCC306.11 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 283
Score = 25.0 bits (52), Expect = 9.5
Identities = 11/32 (34%), Positives = 16/32 (50%)
Frame = -1
Query: 595 VFFYYDSAQDWSTTHTLHSYIQVYKIRRVFDG 500
+ F DS+ DWS TL + Y + F+G
Sbjct: 181 ILFTRDSSMDWSCNMTLCNTDYPYLVNTCFNG 212
>SPAC23A1.12c |||phenylalanine-tRNA ligase beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 589
Score = 25.0 bits (52), Expect = 9.5
Identities = 11/39 (28%), Positives = 23/39 (58%)
Frame = +3
Query: 387 CAHNSSHSGETRQVGRTTSVTIKNPRYVTFFVLMQGLLP 503
C+H+ +++ R+ + +V + NP+ + F V+ LLP
Sbjct: 408 CSHDENYAW-LRKTDDSKAVQLANPKTLEFQVVRSSLLP 445
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,760,693
Number of Sequences: 5004
Number of extensions: 59484
Number of successful extensions: 177
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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