Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30214
         (419 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC970.05 |rpl3601|rpl36-1|60S ribosomal protein L36|Schizosacc...    60   2e-10
SPBC405.07 |rpl3602|rpl36-2, rpl36|60S ribosomal protein L36|Sch...    59   2e-10
SPAC4H3.01 |||DNAJ domain protein Caj1/Djp1 type|Schizosaccharom...    31   0.055
SPAC21E11.06 |tif224||translation initiation factor eIF2B delta ...    27   0.90 
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo...    27   1.2  
SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pomb...    27   1.2  
SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor |Schizo...    26   2.7  
SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly sc...    25   4.8  
SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog 1|Schizosa...    25   4.8  
SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr...    25   6.3  
SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1||...    25   6.3  
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo...    25   6.3  
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc...    24   8.4  

>SPCC970.05 |rpl3601|rpl36-1|60S ribosomal protein
           L36|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 99

 Score = 59.7 bits (138), Expect = 2e-10
 Identities = 28/55 (50%), Positives = 39/55 (70%)
 Frame = +1

Query: 88  KGITDKAIRIRPARLKGLQTKHSKFVRDLVREVVGHAQYEKRAMELLKVSKDKRA 252
           K +T + +  RP+R KG  +K + FVR +VREV G A YE+R MEL++ S+DKRA
Sbjct: 13  KVLTKRQLPERPSRRKGQLSKRTSFVRSIVREVAGFAPYERRVMELIRNSQDKRA 67



 Score = 29.9 bits (64), Expect = 0.17
 Identities = 15/28 (53%), Positives = 19/28 (67%)
 Frame = +3

Query: 255 KFLKRRLGTHIRAKRKREELSNVLAQMR 338
           K  K+RLGT  RAK K EEL++V+   R
Sbjct: 69  KLAKKRLGTLKRAKGKIEELTSVIQSSR 96


>SPBC405.07 |rpl3602|rpl36-2, rpl36|60S ribosomal protein
           L36|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 99

 Score = 59.3 bits (137), Expect = 2e-10
 Identities = 28/55 (50%), Positives = 39/55 (70%)
 Frame = +1

Query: 88  KGITDKAIRIRPARLKGLQTKHSKFVRDLVREVVGHAQYEKRAMELLKVSKDKRA 252
           K +T + +  RP+R KG  +K + FVR +VREV G A YE+R MEL++ S+DKRA
Sbjct: 13  KTLTKRQLPERPSRRKGHLSKRTAFVRSIVREVAGFAPYERRVMELIRNSQDKRA 67



 Score = 29.9 bits (64), Expect = 0.17
 Identities = 15/28 (53%), Positives = 19/28 (67%)
 Frame = +3

Query: 255 KFLKRRLGTHIRAKRKREELSNVLAQMR 338
           K  K+RLGT  RAK K EEL++V+   R
Sbjct: 69  KLAKKRLGTLKRAKGKIEELTSVIQSSR 96


>SPAC4H3.01 |||DNAJ domain protein Caj1/Djp1
           type|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 392

 Score = 31.5 bits (68), Expect = 0.055
 Identities = 13/23 (56%), Positives = 17/23 (73%)
 Frame = +3

Query: 231 GVKRQACSKFLKRRLGTHIRAKR 299
           GV R+ACS  LK+R+ T +R KR
Sbjct: 322 GVLREACSNLLKKRVPTELRLKR 344


>SPAC21E11.06 |tif224||translation initiation factor eIF2B delta
           subunit|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 467

 Score = 27.5 bits (58), Expect = 0.90
 Identities = 13/24 (54%), Positives = 16/24 (66%)
 Frame = +1

Query: 301 SVKNLATCSLR*GRQPPRLITITI 372
           SVKNL   SL+    PPRLIT+ +
Sbjct: 421 SVKNLKLLSLKYDVTPPRLITVCV 444


>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 689

 Score = 27.1 bits (57), Expect = 1.2
 Identities = 14/38 (36%), Positives = 22/38 (57%)
 Frame = -1

Query: 365 MVMSLGGCLPHLSEHVAKFFTLPLGADVCAQSSLQELR 252
           +V SL G    L +  A+FFT PL ++VCA + +   +
Sbjct: 544 IVKSLNGEYIALEKIEAQFFTSPLVSNVCAYADVNHAK 581


>SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 990

 Score = 27.1 bits (57), Expect = 1.2
 Identities = 14/42 (33%), Positives = 26/42 (61%), Gaps = 3/42 (7%)
 Frame = +3

Query: 174  STRSCRTRSI*EEGY---GVT*GVKRQACSKFLKRRLGTHIR 290
            +TR+C T+S+ EEG+    V+  +KR+  +  LK R+   ++
Sbjct: 928  ATRACLTQSLEEEGFPSRNVSNEIKRRFLTDLLKARIKDKVK 969


>SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 456

 Score = 25.8 bits (54), Expect = 2.7
 Identities = 10/30 (33%), Positives = 20/30 (66%)
 Frame = -1

Query: 269 SLQELRARLSFDTLSNSIALFSY*ACPTTS 180
           SL    + +S+D+LS+ +++ S+  CP T+
Sbjct: 71  SLSSSDSIMSYDSLSDDVSVLSFLDCPLTT 100


>SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly
           scaffold protein Isu1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 192

 Score = 25.0 bits (52), Expect = 4.8
 Identities = 13/35 (37%), Positives = 18/35 (51%)
 Frame = +1

Query: 118 RPARLKGLQTKHSKFVRDLVREVVGHAQYEKRAME 222
           RPA L+ L+T  SKFV  +    V    Y K  ++
Sbjct: 27  RPANLQFLKTNSSKFVPQVTAN-VSRRMYHKNVLD 60


>SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog
           1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 941

 Score = 25.0 bits (52), Expect = 4.8
 Identities = 12/29 (41%), Positives = 19/29 (65%)
 Frame = -1

Query: 233 TLSNSIALFSY*ACPTTSRTKSRTNLECF 147
           T+++S  LF+  A   T+ TKS  N+EC+
Sbjct: 850 TINHSRTLFATHAHQLTNLTKSFKNVECY 878


>SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1060

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 13/43 (30%), Positives = 20/43 (46%)
 Frame = -1

Query: 341 LPHLSEHVAKFFTLPLGADVCAQSSLQELRARLSFDTLSNSIA 213
           LP+ S   + FF LP      A +S  ++       +L N+IA
Sbjct: 284 LPNASGSSSSFFGLPTAVSASAGTSFSDISPAAPKASLENNIA 326


>SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1101

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 13/30 (43%), Positives = 17/30 (56%)
 Frame = -3

Query: 96  DTLAASGYFSCFVAFSQAYCDFKTRSHDFG 7
           D   A  + S F AFSQ Y  +  +S+DFG
Sbjct: 319 DLRLACCFESHFFAFSQHYPRYLEQSNDFG 348


>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1183

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 9/24 (37%), Positives = 19/24 (79%)
 Frame = +3

Query: 264 KRRLGTHIRAKRKREELSNVLAQM 335
           K+RL  +I  K K+EE+++++A++
Sbjct: 89  KKRLDKYIENKLKKEEVASLIAKL 112


>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1576

 Score = 24.2 bits (50), Expect = 8.4
 Identities = 9/26 (34%), Positives = 17/26 (65%)
 Frame = +1

Query: 184  VVGHAQYEKRAMELLKVSKDKRALSS 261
            ++ HA      ++ L+VSKDKR +++
Sbjct: 1119 LINHADILSSILDYLQVSKDKRRMAT 1144


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,468,242
Number of Sequences: 5004
Number of extensions: 25977
Number of successful extensions: 74
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 72
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 74
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 148351622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)