Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30209
(686 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g62290.1 68414.m07027 aspartyl protease family protein contai... 82 3e-16
At4g04460.1 68417.m00648 aspartyl protease family protein contai... 80 2e-15
At1g11910.1 68414.m01374 aspartyl protease family protein contai... 75 6e-14
At1g69100.1 68414.m07907 aspartyl protease family protein contai... 45 5e-05
At4g22050.1 68417.m03189 aspartyl protease family protein contai... 39 0.004
At2g15770.1 68415.m01808 glycine-rich protein contains a domain ... 31 0.95
At5g53870.1 68418.m06701 plastocyanin-like domain-containing pro... 30 1.7
At1g30730.1 68414.m03756 FAD-binding domain-containing protein s... 30 1.7
At1g03080.1 68414.m00282 kinase interacting family protein simil... 29 2.2
At5g10720.1 68418.m01242 sensory transduction histidine kinase-r... 29 2.9
At3g47850.1 68416.m05216 expressed protein 29 2.9
At1g05830.1 68414.m00610 trithorax protein, putative / PHD finge... 29 2.9
At4g35785.1 68417.m05082 transformer serine/arginine-rich ribonu... 29 3.8
At3g56590.1 68416.m06293 hydroxyproline-rich glycoprotein family... 29 3.8
At1g64350.1 68414.m07292 transducin family protein / WD-40 repea... 29 3.8
At3g55460.1 68416.m06159 SC35-like splicing factor, 30 kD (SCL30... 28 5.0
At3g08670.1 68416.m01007 expressed protein 28 6.7
At4g19390.1 68417.m02854 expressed protein 27 8.8
At3g08680.2 68416.m01009 leucine-rich repeat transmembrane prote... 27 8.8
At3g08680.1 68416.m01008 leucine-rich repeat transmembrane prote... 27 8.8
At1g06820.1 68414.m00727 carotenoid isomerase, putative similar ... 27 8.8
>At1g62290.1 68414.m07027 aspartyl protease family protein contains
Pfam profiles: PF00026 eukaryotic aspartyl protease,
PF03489 surfactant protein B, PF05184 saposin-like type
B, region 1
Length = 513
Score = 82.2 bits (194), Expect = 3e-16
Identities = 40/84 (47%), Positives = 52/84 (61%), Gaps = 1/84 (1%)
Frame = +2
Query: 2 EAVSXPGLAFVAAKFDGILGMAFSTIAVDXVTPVFDNMVAQGLV-QPVFSFYLNXXPGAT 178
E S PGL F+ AKFDG+LG+ F IAV TPV+ NM+ QGL+ +PVFSF+LN P +
Sbjct: 179 ETTSEPGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSE 238
Query: 179 TXXXXXXXXSDPAHYRGDLVRVPL 250
DP H+RG+ VP+
Sbjct: 239 EGGEIVFGGVDPKHFRGEHTFVPV 262
Score = 77.4 bits (182), Expect = 8e-15
Identities = 33/58 (56%), Positives = 42/58 (72%)
Frame = +3
Query: 510 DYVLRVAQFGHTVCLXGFMALDVPKPMGPLWILGDVFIGKYYTEFRRGERQLGFAAAV 683
+YVL++ + C+ GF ALD+P P GPLWILGDVF+GKY+T F G Q+GFA AV
Sbjct: 456 EYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEAV 513
Score = 58.8 bits (136), Expect = 3e-09
Identities = 26/57 (45%), Positives = 38/57 (66%), Gaps = 2/57 (3%)
Frame = +1
Query: 262 YWEFHMDSVNV--NASRFCXQGCSAIADTGTSLIAGPSKEVEALNAAVGATAIAFGQ 426
YW+F M V + ++ +C GCSAIAD+GTSL+AGP+ V +N A+GA+ + Q
Sbjct: 267 YWQFDMGEVLIAGESTGYCGSGCSAIADSGTSLLAGPTAVVAMINKAIGASGVVSQQ 323
Score = 31.1 bits (67), Expect = 0.72
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +1
Query: 421 GQYVVDCSLIPHLPRVTFTIAGNDFTL 501
G+ VDCS + +P V+FTI G F L
Sbjct: 426 GESAVDCSQLSKMPTVSFTIGGKVFDL 452
>At4g04460.1 68417.m00648 aspartyl protease family protein contains
Pfam profiles: PF00026 eukaryotic aspartyl protease,
PF03489 surfactant protein B, PF05184 saposin-like type
B, region 1
Length = 508
Score = 79.8 bits (188), Expect = 2e-15
Identities = 43/101 (42%), Positives = 56/101 (55%), Gaps = 1/101 (0%)
Frame = +2
Query: 2 EAVSXPGLAFVAAKFDGILGMAFSTIAVDXVTPVFDNMVAQGLV-QPVFSFYLNXXPGAT 178
EA S PG+ F+ AKFDGILG+ F I+V TPV+ NMV +GLV +P+FSF+LN P
Sbjct: 177 EATSEPGITFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRNPKDP 236
Query: 179 TXXXXXXXXSDPAHYRGDLVRVPLLRTRTGSSTWTLSMSTL 301
DP H++G+ VP+ T W M L
Sbjct: 237 EGGEIVFGGVDPKHFKGEHTFVPV----THKGYWQFDMGDL 273
Score = 66.9 bits (156), Expect = 1e-11
Identities = 26/57 (45%), Positives = 39/57 (68%)
Frame = +3
Query: 510 DYVLRVAQFGHTVCLXGFMALDVPKPMGPLWILGDVFIGKYYTEFRRGERQLGFAAA 680
DY+ ++ + + C GF A+D+ P GPLWILGD+F+G Y+T F G+ ++GFA A
Sbjct: 451 DYIFKIGEGVESQCTSGFTAMDIAPPRGPLWILGDIFMGPYHTVFDYGKGRVGFAKA 507
Score = 56.4 bits (130), Expect = 2e-08
Identities = 24/53 (45%), Positives = 35/53 (66%), Gaps = 2/53 (3%)
Frame = +1
Query: 262 YWEFHMDSVNV--NASRFCXQGCSAIADTGTSLIAGPSKEVEALNAAVGATAI 414
YW+F M + + + +C +GCSAIAD+GTSL+ GPS + +N A+GA I
Sbjct: 265 YWQFDMGDLQIAGKPTGYCAKGCSAIADSGTSLLTGPSTVITMINHAIGAQGI 317
Score = 29.1 bits (62), Expect = 2.9
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +1
Query: 424 QYVVDCSLIPHLPRVTFTIAGNDFTL 501
Q VDC + +P VTF+I G F L
Sbjct: 422 QSAVDCGRVSSMPIVTFSIGGRSFDL 447
>At1g11910.1 68414.m01374 aspartyl protease family protein contains
Pfam profiles: PF00026 eukaryotic aspartyl protease,
PF03489 surfactant protein B, PF05184 saposin-like type
B, region 1
Length = 506
Score = 74.5 bits (175), Expect = 6e-14
Identities = 37/84 (44%), Positives = 48/84 (57%), Gaps = 1/84 (1%)
Frame = +2
Query: 2 EAVSXPGLAFVAAKFDGILGMAFSTIAVDXVTPVFDNMVAQGLV-QPVFSFYLNXXPGAT 178
EA PG+ FV AKFDGILG+ F I+V PV+ NM+ QGL+ +PVFSF+LN
Sbjct: 172 EATKEPGITFVVAKFDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRNADEE 231
Query: 179 TXXXXXXXXSDPAHYRGDLVRVPL 250
DP H++G VP+
Sbjct: 232 EGGELVFGGVDPNHFKGKHTYVPV 255
Score = 74.1 bits (174), Expect = 8e-14
Identities = 33/57 (57%), Positives = 41/57 (71%)
Frame = +3
Query: 510 DYVLRVAQFGHTVCLXGFMALDVPKPMGPLWILGDVFIGKYYTEFRRGERQLGFAAA 680
+YVL+V + C+ GF+ALDV P GPLWILGDVF+GKY+T F G Q+GFA A
Sbjct: 449 EYVLKVGEGPVAQCISGFIALDVAPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 505
Score = 58.0 bits (134), Expect = 5e-09
Identities = 26/57 (45%), Positives = 36/57 (63%), Gaps = 2/57 (3%)
Frame = +1
Query: 262 YWEFHMDSVNVNASR--FCXQGCSAIADTGTSLIAGPSKEVEALNAAVGATAIAFGQ 426
YW+F M V + + FC GCSAIAD+GTSL+AGP+ + +N A+GA + Q
Sbjct: 260 YWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPTTIITMINHAIGAAGVVSQQ 316
>At1g69100.1 68414.m07907 aspartyl protease family protein contains
Pfam profile: PF00026 eukaryotic aspartyl protease
Length = 343
Score = 44.8 bits (101), Expect = 5e-05
Identities = 26/86 (30%), Positives = 41/86 (47%), Gaps = 4/86 (4%)
Frame = +1
Query: 256 DTYWEFHMDSVNVN---ASRFCXQ-GCSAIADTGTSLIAGPSKEVEALNAAVGATAIAFG 423
D W+ M + +N A FC C+A+ D+G++ I GP + V + +GAT +
Sbjct: 203 DDRWKIKMSKIYINGKPAINFCDDVECTAMVDSGSTDIFGPDEAVGKIYKEIGATKV--- 259
Query: 424 QYVVDCSLIPHLPRVTFTIAGNDFTL 501
++ C P LP + F I G L
Sbjct: 260 --IIRCEQFPALPDIYFEIGGKHLRL 283
>At4g22050.1 68417.m03189 aspartyl protease family protein contains
Pfam profile: PF00026 eukaryotic aspartyl protease
Length = 354
Score = 38.7 bits (86), Expect = 0.004
Identities = 22/67 (32%), Positives = 31/67 (46%)
Frame = +1
Query: 301 SRFCXQGCSAIADTGTSLIAGPSKEVEALNAAVGATAIAFGQYVVDCSLIPHLPRVTFTI 480
+ C GC AI D+G+S I P + ++ +G +C+ LP VTFTI
Sbjct: 239 TNICSSGCKAIVDSGSSNINVPMDSADEIHRYIGVEP--------NCNNFETLPDVTFTI 290
Query: 481 AGNDFTL 501
G F L
Sbjct: 291 GGKAFVL 297
Score = 33.5 bits (73), Expect = 0.13
Identities = 25/71 (35%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
Frame = +2
Query: 29 FVAAKFDGILGMAFSTIAVDXVTPVFDNMVAQG-LVQPVFSFYLN--XXPGATTXXXXXX 199
F FDGILG+ F T ++ T V+ +MV QG + + VFS +L G
Sbjct: 145 FKKMPFDGILGLRF-TDPLNFGTSVWHSMVFQGKIAKNVFSIWLRRFSNSGEINGGEVVF 203
Query: 200 XXSDPAHYRGD 232
PAH+ GD
Sbjct: 204 GGIIPAHFSGD 214
>At2g15770.1 68415.m01808 glycine-rich protein contains a domain
related to blue copper-binding protein; similar to
Stellacyanin (SP:P00302) {Rhus vernicifera}
Length = 301
Score = 30.7 bits (66), Expect = 0.95
Identities = 16/58 (27%), Positives = 26/58 (44%)
Frame = +1
Query: 505 GTTTCSGWHNSGTRCACXXSWRWTCRSPWARCGSWGTCSSASTTRSFDAGNASSGSRP 678
G+ SG +S SW W+ +S W+ SWG+ + S + D+G+ P
Sbjct: 88 GSNRGSGSGSSTNPDGSRRSWNWSLKSGWS--WSWGSNDNDSNSSGSDSGSGLDRETP 143
>At5g53870.1 68418.m06701 plastocyanin-like domain-containing
protein contains similarity to SP|Q02917 Early nodulin
55-2 precursor {Glycine max}; PF02298: Plastocyanin-like
domain
Length = 370
Score = 29.9 bits (64), Expect = 1.7
Identities = 24/89 (26%), Positives = 34/89 (38%), Gaps = 2/89 (2%)
Frame = +2
Query: 404 PRPSPSGSTSWTAASYRTCRESPLPSPGTTSRSRARL--RAPGGTIRAHGVPVRXHGAGR 577
P SPS +TS + A+ +SP P + S S A +P T H
Sbjct: 192 PALSPSHATSHSPATPSPSPKSPSPVSHSPSHSPAHTPSHSPAHTPSHSPAHAPSHSPAH 251
Query: 578 AEAHGPAVDPGGRVHRQVLHGVSTRGTPA 664
A +H PA P H +T +P+
Sbjct: 252 APSHSPAHAPSHSPAHSPSHSPATPKSPS 280
>At1g30730.1 68414.m03756 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 526
Score = 29.9 bits (64), Expect = 1.7
Identities = 22/85 (25%), Positives = 37/85 (43%), Gaps = 6/85 (7%)
Frame = +3
Query: 447 HTAP--AASHLYHRRERLHARGHDY----VLRVAQFGHTVCLXGFMALDVPKPMGPLWIL 608
H +P A + L+ + R+ + GHD+ A F + L F ++DV G W+
Sbjct: 87 HISPVLACARLFPVQVRIRSGGHDFEGLSYTSTAPF-FLIDLLNFKSVDVNLTEGTAWVD 145
Query: 609 GDVFIGKYYTEFRRGERQLGFAAAV 683
+G+ Y + LGF A +
Sbjct: 146 TGATLGELYYKIAEKSNVLGFPAGL 170
>At1g03080.1 68414.m00282 kinase interacting family protein similar to
kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 1744
Score = 29.5 bits (63), Expect = 2.2
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = -3
Query: 543 CARIVPPGARSRALEREVVPGDGKGDSRQVRYEAAVHDVL 424
C +I+ G ++ E++PG G GD R + +HD+L
Sbjct: 935 CIKILRTGIYQVLMKLEIIPGIGSGDENS-RDQRNMHDIL 973
>At5g10720.1 68418.m01242 sensory transduction histidine
kinase-related similar to Sensor protein rcsC (Capsular
synthesis regulator component C) (SP:Q56128) {Salmonella
typhi}; sensory transduction histidine kinase slr1759,
Synechocystis sp., PIR:S75142
Length = 950
Score = 29.1 bits (62), Expect = 2.9
Identities = 17/46 (36%), Positives = 27/46 (58%), Gaps = 4/46 (8%)
Frame = -1
Query: 449 MRLQSTTYCPKAMA--VAPTAA--LRASTSLEGPAISDVPVSAMAE 324
MRL++T + P+ + V TAA L+ S +LEG DVP+ + +
Sbjct: 429 MRLEATKFRPREVVKHVLQTAAASLKKSLTLEGNIADDVPIEVVGD 474
>At3g47850.1 68416.m05216 expressed protein
Length = 322
Score = 29.1 bits (62), Expect = 2.9
Identities = 16/52 (30%), Positives = 26/52 (50%), Gaps = 5/52 (9%)
Frame = +2
Query: 383 PSTRRWAP-----RPSPSGSTSWTAASYRTCRESPLPSPGTTSRSRARLRAP 523
P T RW+P +P P+ + S + +Y SP GT+ +RL++P
Sbjct: 269 PHTSRWSPLVGPSQPGPAVTVSASGTNYIASSPSPAGFGGTSVFRESRLQSP 320
>At1g05830.1 68414.m00610 trithorax protein, putative / PHD finger
family protein / SET domain-containing protein similar
to trithorax-like protein 1 [Arabidopsis thaliana]
GI:12659210; contains Pfam domain, PF00628: PHD-finger
and PF00856: SET domain
Length = 1056
Score = 29.1 bits (62), Expect = 2.9
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +3
Query: 438 LQPHTAPAASHLYHRRERLHARGHDYVLRVA 530
LQPH P H+Y RR+R R + L +A
Sbjct: 74 LQPHRRPEIVHVYCRRKRRRRRRRESFLELA 104
>At4g35785.1 68417.m05082 transformer serine/arginine-rich
ribonucleoprotein, putative similar to transformer-SR
ribonucleoprotein [Nicotiana tabacum]
gi|1781299|emb|CAA70700
Length = 140
Score = 28.7 bits (61), Expect = 3.8
Identities = 20/56 (35%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
Frame = +2
Query: 494 SRSRARLRAPGGTIRAHGVPVRXHGAGRAEAHGPAVD-PGGRVHRQVLHGVSTRGT 658
SRSR RLR+ ++ P R G R+ + G V+ PG ++ + G+STR T
Sbjct: 31 SRSRPRLRSRSRSLPRPVSPSRSRGRSRSRSRGSEVENPGTTLY---VTGLSTRVT 83
>At3g56590.1 68416.m06293 hydroxyproline-rich glycoprotein family
protein
Length = 477
Score = 28.7 bits (61), Expect = 3.8
Identities = 21/77 (27%), Positives = 29/77 (37%)
Frame = +2
Query: 401 APRPSPSGSTSWTAASYRTCRESPLPSPGTTSRSRARLRAPGGTIRAHGVPVRXHGAGRA 580
AP PS S T A + + SPLP P + R G + H R+
Sbjct: 348 APEPSLSPPTKGFAPASAPTKHSPLP-PRNPPCPYEQRRPKGNSALNHHTAPPTPAPHRS 406
Query: 581 EAHGPAVDPGGRVHRQV 631
+ H PA +P H +
Sbjct: 407 QPHPPAPNPAPPRHHAI 423
>At1g64350.1 68414.m07292 transducin family protein / WD-40 repeat
family protein contains 5 WD-40 repeats (PF00400);
similar to nuclear pore protein SEH1 (SP:P53011)
[Saccharomyces cerevisiae]
Length = 326
Score = 28.7 bits (61), Expect = 3.8
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = +1
Query: 496 TLEGTTTCSGWHNSGTRCA 552
TL+ TTCS W+ SG R A
Sbjct: 7 TLDSGTTCSSWNQSGDRLA 25
>At3g55460.1 68416.m06159 SC35-like splicing factor, 30 kD (SCL30)
nearly identical to SC35-like splicing factor SCL30, 30
kD [Arabidopsis thaliana] GI:9843657;
Serine/arginine-rich protein/putative splicing factor,
Arabidopdis thaliana, EMBL:AF099940; contains Pfam
profile PF00076: RNA recognition motif. (a.k.a. RRM,
RBD, or RNP domain)
Length = 262
Score = 28.3 bits (60), Expect = 5.0
Identities = 16/39 (41%), Positives = 18/39 (46%)
Frame = +1
Query: 4 GRVGXRACLRGRQVRRDPRDGLQHYRSGXCDPGVRQHGG 120
GR R+ R R RR P D YRS P R+ GG
Sbjct: 153 GRSRSRSISRSRSPRR-PSDSRSRYRSRSYSPAPRRRGG 190
>At3g08670.1 68416.m01007 expressed protein
Length = 567
Score = 27.9 bits (59), Expect = 6.7
Identities = 21/54 (38%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +2
Query: 380 KPSTRRWAP-RPSPSGSTSWTAASYRTCRESPLPSPGTTSRSRARLRAPGGTIR 538
+P++R P R SPS STS +A S T S G T S +R +PG +R
Sbjct: 264 RPNSRPSTPTRRSPS-STSLSATSGPTISGGRAASNGRTGPSLSRPSSPGPRVR 316
>At4g19390.1 68417.m02854 expressed protein
Length = 273
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +1
Query: 541 TRCACXXSWRWTCRSPWARCGSWGTCSSASTTRS 642
T+ W W+ RSP R S T +SAST+ S
Sbjct: 51 TKTITSSDWSWSYRSP-GRLASASTSTSASTSTS 83
>At3g08680.2 68416.m01009 leucine-rich repeat transmembrane protein
kinase, putative contains Pfam profile: PF00069
Eukaryotic protein kinase domain, PF00560 leucine Rich
Repeat (5 copies)
Length = 640
Score = 27.5 bits (58), Expect = 8.8
Identities = 22/56 (39%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
Frame = -1
Query: 509 VPSSVKSFPA-MVKVTRGRCGMRLQSTTYCPKAMAVAPTAALRASTSLEGPAISDV 345
VPSSVKSFPA + CG L T CP+ AP+ + +T EGP +++
Sbjct: 199 VPSSVKSFPASSFQGNSLLCGAPL---TPCPE-NTTAPSPS--PTTPTEGPGTTNI 248
>At3g08680.1 68416.m01008 leucine-rich repeat transmembrane protein
kinase, putative contains Pfam profile: PF00069
Eukaryotic protein kinase domain, PF00560 leucine Rich
Repeat (5 copies)
Length = 640
Score = 27.5 bits (58), Expect = 8.8
Identities = 22/56 (39%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
Frame = -1
Query: 509 VPSSVKSFPA-MVKVTRGRCGMRLQSTTYCPKAMAVAPTAALRASTSLEGPAISDV 345
VPSSVKSFPA + CG L T CP+ AP+ + +T EGP +++
Sbjct: 199 VPSSVKSFPASSFQGNSLLCGAPL---TPCPE-NTTAPSPS--PTTPTEGPGTTNI 248
>At1g06820.1 68414.m00727 carotenoid isomerase, putative similar to
carotenoid isomerase from Lycopersicon esculentum
[gi:19550437]; contains Pfam profile: PF02032 Phytoene
dehydrogenase related enzyme
Length = 595
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +2
Query: 50 GILGMAFSTIAVDXVTPVFDN-MVAQGLVQPVFS 148
G+LGM F+T A+D + V D+ QG++ FS
Sbjct: 526 GLLGMPFNTTAIDGLYCVGDSCFPGQGVIAVAFS 559
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,792,413
Number of Sequences: 28952
Number of extensions: 278370
Number of successful extensions: 980
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 912
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 973
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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