Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30198
(385 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70... 75 1e-14
At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3 (HS... 72 1e-13
At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1 (HS... 71 2e-13
At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2 (HS... 71 4e-13
At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70... 70 6e-13
At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, puta... 65 2e-11
At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)... 64 4e-11
At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)... 64 4e-11
At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)... 64 4e-11
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)... 57 4e-09
At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp... 30 0.46
At1g14640.1 68414.m01740 SWAP (Suppressor-of-White-APricot)/surp... 28 1.9
At3g59960.1 68416.m06692 SET domain-containing protein low simil... 27 3.3
At5g01570.1 68418.m00072 hypothetical protein hypothetical prote... 27 4.3
At2g44710.1 68415.m05564 RNA recognition motif (RRM)-containing ... 27 4.3
At3g19050.1 68416.m02420 kinesin motor protein-related contains ... 27 5.7
At3g16565.1 68416.m02118 alanyl-tRNA synthetase-related low simi... 27 5.7
At1g44910.1 68414.m05146 FF domain-containing protein / WW domai... 26 9.9
At1g27920.1 68414.m03421 microtubule associated protein (MAP65/A... 26 9.9
At1g11730.1 68414.m01346 galactosyltransferase family protein co... 26 9.9
>At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70,
putative similar to heat shock protein hsp70 GI:1771478
from [Pisum sativum]
Length = 646
Score = 75.4 bits (177), Expect = 1e-14
Identities = 32/56 (57%), Positives = 44/56 (78%)
Frame = +2
Query: 2 KLKEKISDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGICNPIITKMYQ 169
KL +K++ DKQ I + TI+W++ NQLA+ +E+E+K KELEGICNPII+KMYQ
Sbjct: 562 KLAQKLTQEDKQKIEKAIDETIEWIEGNQLAEVDEFEYKLKELEGICNPIISKMYQ 617
>At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3
(HSC70-3) (HSP70-3) identical to SP|O65719 Heat shock
cognate 70 kDa protein 3 (Hsc70.3) {Arabidopsis
thaliana}
Length = 649
Score = 72.1 bits (169), Expect = 1e-13
Identities = 31/56 (55%), Positives = 41/56 (73%)
Frame = +2
Query: 2 KLKEKISDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGICNPIITKMYQ 169
K+ EK++ DK+ I D I+WL++NQLA+ +E+E K KELE ICNPII KMYQ
Sbjct: 563 KIGEKLAGDDKKKIEDSIEAAIEWLEANQLAECDEFEDKMKELESICNPIIAKMYQ 618
>At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1
(HSC70-1) (HSP70-1) identical to SP|P22953 Heat shock
cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
thaliana}
Length = 651
Score = 71.3 bits (167), Expect = 2e-13
Identities = 31/56 (55%), Positives = 40/56 (71%)
Frame = +2
Query: 2 KLKEKISDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGICNPIITKMYQ 169
K+ EK+ +DK+ I D I+WL+ NQLA+ +E+E K KELE ICNPII KMYQ
Sbjct: 563 KIGEKLPAADKKKIEDSIEQAIQWLEGNQLAEADEFEDKMKELESICNPIIAKMYQ 618
>At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2
(HSC70-2) (HSP70-2) identical to SP|P22954 Heat shock
cognate 70 kDa protein 2 (Hsc70.2) {Arabidopsis
thaliana}
Length = 653
Score = 70.5 bits (165), Expect = 4e-13
Identities = 29/56 (51%), Positives = 39/56 (69%)
Frame = +2
Query: 2 KLKEKISDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGICNPIITKMYQ 169
K+ EK+ +DK+ + D I+WLD NQL + +E+E K KELE +CNPII KMYQ
Sbjct: 563 KIGEKLPAADKKKVEDSIEEAIQWLDGNQLGEADEFEDKMKELESVCNPIIAKMYQ 618
>At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein
GI:425194 [Spinacia oleracea]
Length = 650
Score = 69.7 bits (163), Expect = 6e-13
Identities = 29/56 (51%), Positives = 40/56 (71%)
Frame = +2
Query: 2 KLKEKISDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGICNPIITKMYQ 169
K+ K+ +DK+ I D + I+WLD NQLA+ +E+E K KELE +CNPII +MYQ
Sbjct: 563 KIASKLDAADKKKIEDAIDQAIEWLDGNQLAEADEFEDKMKELESLCNPIIARMYQ 618
>At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein,
putative / HSC70, putative / HSP70, putative strong
similarity to heat shock cognate 70 kd protein 1
SP:P22953 [Arabidopsis thaliana (Mouse-ear cress)]
Length = 617
Score = 64.9 bits (151), Expect = 2e-11
Identities = 28/53 (52%), Positives = 37/53 (69%)
Frame = +2
Query: 11 EKISDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGICNPIITKMYQ 169
EK+ +DK+ D I+WLD NQLA+ +E+EHK KELE + + IITKMYQ
Sbjct: 564 EKLPAADKKKFEDSIEEVIQWLDDNQLAEADEFEHKMKELESVWSTIITKMYQ 616
>At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 613
Score = 63.7 bits (148), Expect = 4e-11
Identities = 26/56 (46%), Positives = 38/56 (67%)
Frame = +2
Query: 2 KLKEKISDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGICNPIITKMYQ 169
KL +K+ +K+ I ++WLD NQ ++KEEY+ K KE+E +CNPIIT +YQ
Sbjct: 534 KLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 589
>At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 668
Score = 63.7 bits (148), Expect = 4e-11
Identities = 26/56 (46%), Positives = 38/56 (67%)
Frame = +2
Query: 2 KLKEKISDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGICNPIITKMYQ 169
KL +K+ +K+ I ++WLD NQ ++KEEY+ K KE+E +CNPIIT +YQ
Sbjct: 589 KLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 644
>At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)
SWISS-PROT:Q9LKR3 PMID:8888624
Length = 669
Score = 63.7 bits (148), Expect = 4e-11
Identities = 26/56 (46%), Positives = 38/56 (67%)
Frame = +2
Query: 2 KLKEKISDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGICNPIITKMYQ 169
KL +K+ +K+ I ++WLD NQ ++KEEY+ K KE+E +CNPIIT +YQ
Sbjct: 589 KLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 644
>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
Similar to Arabidopsis luminal binding protein
(gb|D89342); contains Pfam domain PF00012: dnaK protein
Length = 678
Score = 57.2 bits (132), Expect = 4e-09
Identities = 23/56 (41%), Positives = 38/56 (67%)
Frame = +2
Query: 2 KLKEKISDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGICNPIITKMYQ 169
KL +KISD DK+ + ++WL+ N A+KE+Y+ K KE+E +C+P+I +Y+
Sbjct: 603 KLAKKISDEDKEKMEGVLKEALEWLEENVNAEKEDYDEKLKEVELVCDPVIKSVYE 658
>At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein / ubiquitin family protein
similar to SP|Q15459 Splicing factor 3 subunit 1
(Spliceosome associated protein 114) {Homo sapiens};
contains Pfam profiles PF00240: Ubiquitin family,
PF01805: Surp module
Length = 785
Score = 30.3 bits (65), Expect = 0.46
Identities = 18/47 (38%), Positives = 30/47 (63%), Gaps = 2/47 (4%)
Frame = +2
Query: 5 LKEKI--SDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGI 139
LKEK+ S +D T+L++C ++W D +Q K++ E +KELE +
Sbjct: 252 LKEKLRKSAADLTTVLERCLHRLEW-DRSQEQQKKK-EEDEKELERV 296
>At1g14640.1 68414.m01740 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein similar to human splicing
factor GB:CAA59494 GI:899298 from [Homo sapiens];
contains Pfam profile PF01805: Surp module
Length = 735
Score = 28.3 bits (60), Expect = 1.9
Identities = 14/43 (32%), Positives = 24/43 (55%), Gaps = 2/43 (4%)
Frame = +2
Query: 5 LKEKISDS--DKQTILDKCNTTIKWLDSNQLADKEEYEHKQKE 127
LKEK+ S D T+L++C ++W + +E + K+KE
Sbjct: 243 LKEKLRKSVADLTTVLERCLNRLEWDRFQEEEKNKEEDEKEKE 285
>At3g59960.1 68416.m06692 SET domain-containing protein low
similarity to huntingtin interacting protein 1 [Homo
sapiens] GI:12697196; contains Pfam profile PF00856: SET
domain
Length = 352
Score = 27.5 bits (58), Expect = 3.3
Identities = 12/26 (46%), Positives = 18/26 (69%), Gaps = 1/26 (3%)
Frame = -3
Query: 170 PGTSSLL-SDCKCLPILSACAHTPPC 96
PG+S+L SDC C +LS+C+ + C
Sbjct: 71 PGSSTLCGSDCNCGILLSSCSSSCKC 96
>At5g01570.1 68418.m00072 hypothetical protein hypothetical protein
T16O11.19 - Arabidopsis thaliana, EMBL:AC010871
Length = 157
Score = 27.1 bits (57), Expect = 4.3
Identities = 16/44 (36%), Positives = 25/44 (56%)
Frame = +2
Query: 2 KLKEKISDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELE 133
++ E + D K+ LD C +S ++AD+EE E QKEL+
Sbjct: 51 EIHESLQDLQKK--LDVCKEKTDEANS-EIADEEEIERLQKELD 91
>At2g44710.1 68415.m05564 RNA recognition motif (RRM)-containing
protein
Length = 809
Score = 27.1 bits (57), Expect = 4.3
Identities = 10/26 (38%), Positives = 13/26 (50%)
Frame = +3
Query: 165 TRVPEESPEVCRASRAEHPEPEVPPP 242
+R P S + SR P P +PPP
Sbjct: 521 SRAPSSSAKRASGSRGRRPRPPLPPP 546
>At3g19050.1 68416.m02420 kinesin motor protein-related contains Pfam
profile: PF00225 Kinesin motor domain; contains
non-consensus splice site (GC) at intron 12
Length = 2722
Score = 26.6 bits (56), Expect = 5.7
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +2
Query: 26 SDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGICNPI 151
+D++T + + + I L+ + A EY HK KELE + +
Sbjct: 2336 ADQKTEITQLSEHISELNLHAEAQASEYMHKFKELEAMAEQV 2377
>At3g16565.1 68416.m02118 alanyl-tRNA synthetase-related low
similarity to SP|Q56273 Alanyl-tRNA synthetase (EC
6.1.1.7) (Alanine--tRNA ligase) (AlaRS) {Thiobacillus
ferrooxidans}
Length = 271
Score = 26.6 bits (56), Expect = 5.7
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +2
Query: 98 KEEYEHKQKELEGICNPIITK 160
+EE++ KQKELE N +I+K
Sbjct: 172 QEEFQVKQKELEAEANELISK 192
>At1g44910.1 68414.m05146 FF domain-containing protein / WW
domain-containing protein contains Pfam profiles
PF01846: FF domain, PF00397: WW domain
Length = 946
Score = 25.8 bits (54), Expect = 9.9
Identities = 13/46 (28%), Positives = 27/46 (58%)
Frame = +2
Query: 2 KLKEKISDSDKQTILDKCNTTIKWLDSNQLADKEEYEHKQKELEGI 139
K+++++ D D+ + L+K + I + + +KEE E K+ E E +
Sbjct: 561 KIQDRLEDDDRCSCLEKIDRLIGFEEYILDLEKEEEELKRVEKEHV 606
>At1g27920.1 68414.m03421 microtubule associated protein
(MAP65/ASE1) family protein low similarity to protein
regulating cytokinesis 1 (PRC1) [Homo sapiens]
GI:2865521; contains Pfam profile PF03999: Microtubule
associated protein (MAP65/ASE1 family)
Length = 592
Score = 25.8 bits (54), Expect = 9.9
Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = +2
Query: 41 ILDKCNTTIKWLDSNQLAD-KEEYEHKQKELEGICN 145
I+ + +K LD + + KE + KQKELE CN
Sbjct: 332 IIQQAEAEVKRLDQLKASRIKELFIKKQKELEDTCN 367
>At1g11730.1 68414.m01346 galactosyltransferase family protein
contains Pfam profile: PF01762 galactosyltransferase
Length = 384
Score = 25.8 bits (54), Expect = 9.9
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = +2
Query: 2 KLKEKISDSDKQTILDKCNTTIKWLDSN 85
KL SD DK+ +LD N TI LD +
Sbjct: 55 KLSLSSSDCDKKNVLDYGNNTIGILDKS 82
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,488,963
Number of Sequences: 28952
Number of extensions: 137471
Number of successful extensions: 556
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 539
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 556
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 537681456
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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