Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30196
(762 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g25040.1 68416.m03129 ER lumen protein retaining receptor, pu... 93 2e-19
At1g29330.1 68414.m03585 ER lumen protein retaining receptor (ER... 79 3e-15
At1g75760.1 68414.m08799 ER lumen protein retaining receptor fam... 70 2e-12
At3g25160.1 68416.m03141 ER lumen protein retaining receptor fam... 63 2e-10
At1g19970.1 68414.m02502 ER lumen protein retaining receptor fam... 63 2e-10
At2g21190.1 68415.m02514 ER lumen protein retaining receptor fam... 60 2e-09
At4g38790.1 68417.m05492 ER lumen protein retaining receptor fam... 56 2e-08
At4g09360.1 68417.m01545 disease resistance protein (NBS-LRR cla... 29 3.4
At5g44860.1 68418.m05499 expressed protein strong similarity to ... 29 4.5
At2g46970.1 68415.m05867 basic helix-loop-helix (bHLH) protein, ... 28 7.8
>At3g25040.1 68416.m03129 ER lumen protein retaining receptor,
putative / HDEL receptor, putative similar to SP|P35402
ER lumen protein retaining receptor (HDEL receptor)
{Arabidopsis thaliana}; contains Pfam profile PF00810:
ER lumen protein retaining receptor
Length = 215
Score = 92.7 bits (220), Expect = 2e-19
Identities = 44/80 (55%), Positives = 58/80 (72%), Gaps = 1/80 (1%)
Frame = +1
Query: 277 YLMYVK-FKATYDHNHDTFRIEFLLIPTFILALLINHEFTVLEVLWTFSIYLESVAILPQ 453
Y+ Y K TYD DTFR FL++P F+LALLI+ +FT LEVLWT S+YLE+VAILPQ
Sbjct: 77 YMKYHKAVHRTYDREQDTFRHWFLVLPCFLLALLIHEKFTFLEVLWTSSLYLEAVAILPQ 136
Query: 454 LFLVSKTREAESITSHYLLL 513
L L+ +TR +++T Y+ L
Sbjct: 137 LVLLQRTRNIDNLTGQYIFL 156
Score = 74.9 bits (176), Expect = 5e-14
Identities = 34/59 (57%), Positives = 39/59 (66%), Gaps = 1/59 (1%)
Frame = +3
Query: 513 LGSYRALYLLNWVYRYVVESHY-ELIAIISGVVQTILYCDFFYLYITKVLKGKKLQLPA 686
LG YR LY+LNW+YRY E H+ I I+G VQT+LY DFFY Y KKLQLPA
Sbjct: 157 LGGYRGLYILNWIYRYFTEPHFVHWITWIAGFVQTLLYADFFYYYFLSWKNNKKLQLPA 215
Score = 66.5 bits (155), Expect = 2e-11
Identities = 33/67 (49%), Positives = 42/67 (62%)
Frame = +2
Query: 53 MNIFRLLGDXXXXXXXXXXXXXXWKSRSCAGISGKSQILFSVVYTTRYLDLLTTHVSPYN 232
MNIFRL GD +SCAG+S K+Q L+++V+ TRYLD+ T+ VS YN
Sbjct: 1 MNIFRLAGDMTHLASVLVLLLKIHTIKSCAGVSLKTQELYAIVFATRYLDIFTSFVSLYN 60
Query: 233 TVMKLVF 253
T MKLVF
Sbjct: 61 TSMKLVF 67
>At1g29330.1 68414.m03585 ER lumen protein retaining receptor (ERD2)
/ HDEL receptor identical to SP:P35402 ER lumen protein
retaining receptor (HDEL receptor) {Arabidopsis
thaliana}
Length = 215
Score = 79.0 bits (186), Expect = 3e-15
Identities = 32/71 (45%), Positives = 53/71 (74%)
Frame = +1
Query: 298 KATYDHNHDTFRIEFLLIPTFILALLINHEFTVLEVLWTFSIYLESVAILPQLFLVSKTR 477
+ +YD + DTFR +++++ F+L L++N +FTV EV W FSIYLE+VAILPQL L+ ++
Sbjct: 85 RRSYDKDLDTFRHQYVVLACFVLGLILNEKFTVQEVFWAFSIYLEAVAILPQLVLLQRSG 144
Query: 478 EAESITSHYLL 510
+++T Y++
Sbjct: 145 NVDNLTGQYVV 155
Score = 73.7 bits (173), Expect = 1e-13
Identities = 33/61 (54%), Positives = 41/61 (67%), Gaps = 1/61 (1%)
Frame = +3
Query: 507 VALGSYRALYLLNWVYRYVVESHY-ELIAIISGVVQTILYCDFFYLYITKVLKGKKLQLP 683
V LG+YR LY++NW+YRY E H+ IA +SG+VQT LY DFFY Y KL+LP
Sbjct: 155 VFLGAYRGLYIINWIYRYFTEDHFTRWIACVSGLVQTALYADFFYYYYISWKTNTKLKLP 214
Query: 684 A 686
A
Sbjct: 215 A 215
Score = 68.5 bits (160), Expect = 5e-12
Identities = 36/87 (41%), Positives = 53/87 (60%)
Frame = +2
Query: 53 MNIFRLLGDXXXXXXXXXXXXXXWKSRSCAGISGKSQILFSVVYTTRYLDLLTTHVSPYN 232
MNIFR GD + ++SCAGIS K+Q L+++V+ TRYLDL T +VS YN
Sbjct: 1 MNIFRFAGDMSHLISVLILLLKIYATKSCAGISLKTQELYALVFLTRYLDLFTDYVSLYN 60
Query: 233 TVMKLVFTLPPMQLFI*CMSSLRPPMI 313
++MK+VF + + + CM R P++
Sbjct: 61 SIMKIVFIASSLAI-VWCMR--RHPLV 84
>At1g75760.1 68414.m08799 ER lumen protein retaining receptor family
protein similar to SP|P33946 ER lumen protein retaining
receptor 1 (KDEL receptor 1) {Bos taurus}; contains Pfam
profile PF00810: ER lumen protein retaining receptor
Length = 272
Score = 69.7 bits (163), Expect = 2e-12
Identities = 31/86 (36%), Positives = 55/86 (63%), Gaps = 3/86 (3%)
Frame = +1
Query: 259 TSYATIYLMYVKFKATYDHNHDTFRIEFLLIPTFILALLIN---HEFTVLEVLWTFSIYL 429
T+ IY++ K KA+Y + D F I +++IP +L++LI+ H + ++ W F +YL
Sbjct: 113 TTLFVIYMIRFKLKASYMDDKDNFAIYYVVIPCVVLSVLIHPSTHHHIINKISWAFCVYL 172
Query: 430 ESVAILPQLFLVSKTREAESITSHYL 507
E+V++LPQL ++ T+ E T+HY+
Sbjct: 173 EAVSVLPQLRVMQNTKIVEPFTAHYV 198
Score = 30.3 bits (65), Expect = 1.5
Identities = 12/38 (31%), Positives = 25/38 (65%), Gaps = 1/38 (2%)
Frame = +3
Query: 576 YELIAIISGVVQTILYCDFFYLYITKVLKGK-KLQLPA 686
+ ++ ++S +VQT + DF Y Y+ ++ G+ L+LP+
Sbjct: 232 WPIMVLLSEIVQTFILADFCYYYVKSLMGGQLVLRLPS 269
>At3g25160.1 68416.m03141 ER lumen protein retaining receptor family
protein similar to SP|P24390 ER lumen protein retaining
receptor 1 (KDEL receptor 1) {Homo sapiens}; contains
Pfam profile PF00810: ER lumen protein retaining
receptor
Length = 272
Score = 63.3 bits (147), Expect = 2e-10
Identities = 32/81 (39%), Positives = 50/81 (61%), Gaps = 3/81 (3%)
Frame = +1
Query: 274 IYLMYVKFKATYDHNHDTFRIEFLLIPTFILALLINHEFTVL---EVLWTFSIYLESVAI 444
IY++ K KA+Y + DT ++L+P+ ILAL+IN + +LW F +Y ESV++
Sbjct: 120 IYMIRYKLKASYIKSLDTCYNYYVLVPSAILALIINPSTSYSYFHRILWAFCVYTESVSV 179
Query: 445 LPQLFLVSKTREAESITSHYL 507
LPQL L+ + E T+HY+
Sbjct: 180 LPQLRLMQNAQIIEPFTAHYV 200
Score = 31.9 bits (69), Expect = 0.48
Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 12/70 (17%)
Frame = +3
Query: 510 ALGSYRALYLLNWVYRYV-VESHYEL----------IAIISGVVQTILYCDFFYLYITKV 656
ALG R L +W+ + V HY +A+++ +VQT + DF Y Y+ V
Sbjct: 202 ALGIARFLACAHWIIQVVETRGHYLWLLGAGYFWFPVALLAEIVQTFILADFCYYYVKSV 261
Query: 657 LKGK-KLQLP 683
++G+ L++P
Sbjct: 262 MEGQLVLKMP 271
>At1g19970.1 68414.m02502 ER lumen protein retaining receptor family
protein similar to SP|P33946 ER lumen protein retaining
receptor 1 (KDEL receptor 1) {Bos taurus}; contains Pfam
profile PF00810: ER lumen protein retaining receptor
Length = 272
Score = 62.9 bits (146), Expect = 2e-10
Identities = 28/86 (32%), Positives = 50/86 (58%), Gaps = 3/86 (3%)
Frame = +1
Query: 259 TSYATIYLMYVKFKATYDHNHDTFRIEFLLIPTFILALLIN---HEFTVLEVLWTFSIYL 429
T+ +Y++ K + TY + D F I F++IP +L+ I+ + + W F +YL
Sbjct: 113 TTLWVVYMIRFKLRPTYMEDKDNFAIYFVVIPCAVLSFFIHPSTRHHIINRISWAFCVYL 172
Query: 430 ESVAILPQLFLVSKTREAESITSHYL 507
E+V++LPQL ++ T+ E T+HY+
Sbjct: 173 EAVSVLPQLRVMQNTKIVEPFTAHYV 198
Score = 30.7 bits (66), Expect = 1.1
Identities = 13/38 (34%), Positives = 25/38 (65%), Gaps = 1/38 (2%)
Frame = +3
Query: 576 YELIAIISGVVQTILYCDFFYLYITKVLKGK-KLQLPA 686
+ ++ +IS +VQT + DF Y Y+ ++ G+ L+LP+
Sbjct: 232 WPVMVLISEIVQTFILADFCYYYVQSLMGGQLVLRLPS 269
>At2g21190.1 68415.m02514 ER lumen protein retaining receptor family
protein similar to SP|P33948 ER lumen protein retaining
receptor. {Plasmodium falciparum}; contains Pfam profile
PF00810: ER lumen protein retaining receptor
Length = 269
Score = 59.7 bits (138), Expect = 2e-09
Identities = 31/87 (35%), Positives = 49/87 (56%), Gaps = 4/87 (4%)
Frame = +1
Query: 259 TSYATIYLMYVKFKATYDHNHDTFRIEFLLIPTFILALLINHEFTVLEVL----WTFSIY 426
T+ IY++ K TY D F + ++L P +LA+ I H T +L W F +Y
Sbjct: 110 TTLWVIYMIRFNLKTTYMEEKDNFALYYVLAPCVVLAVWI-HPSTSHNILNRISWGFCVY 168
Query: 427 LESVAILPQLFLVSKTREAESITSHYL 507
LE+V++LPQL ++ T+ E T+HY+
Sbjct: 169 LEAVSVLPQLRVMQNTKIVEPFTAHYV 195
Score = 31.1 bits (67), Expect = 0.84
Identities = 15/35 (42%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = +3
Query: 585 IAIISGVVQTILYCDFFYLYITKVLKGK-KLQLPA 686
+ IIS +VQT + DF Y Y+ V G+ L+LP+
Sbjct: 232 MVIISEIVQTFILADFCYYYVKSVFGGQLVLRLPS 266
>At4g38790.1 68417.m05492 ER lumen protein retaining receptor family
protein similar to SP|P35402 ER lumen protein retaining
receptor (HDEL receptor) {Arabidopsis thaliana};
contains Pfam profile PF00810: ER lumen protein
retaining receptor
Length = 273
Score = 56.4 bits (130), Expect = 2e-08
Identities = 26/86 (30%), Positives = 50/86 (58%), Gaps = 3/86 (3%)
Frame = +1
Query: 259 TSYATIYLMYVKFKATYDHNHDTFRIEFLLIPTFILALLINHEFT---VLEVLWTFSIYL 429
T+ I+++ K +A+Y + D F + ++L P LA+ I+ + + + W +YL
Sbjct: 114 TTLWVIFMIRFKLRASYMEDKDNFPLYYVLAPCVALAVFIHPSTSHNIINRISWALCVYL 173
Query: 430 ESVAILPQLFLVSKTREAESITSHYL 507
E+V++LPQL ++ T+ E T+HY+
Sbjct: 174 EAVSVLPQLRVMQNTKIVEPFTAHYV 199
Score = 31.5 bits (68), Expect = 0.63
Identities = 25/70 (35%), Positives = 35/70 (50%), Gaps = 11/70 (15%)
Frame = +3
Query: 510 ALGSYRALYLLNWVY-------RYVVESHYEL---IAIISGVVQTILYCDFFYLYITKVL 659
ALG R L +WV R +V Y L + +IS +VQT + DF Y Y+ V
Sbjct: 201 ALGVARFLSCAHWVLQVVDTRGRLLVALGYGLWPSMVLISEIVQTFILADFCYYYVKSVF 260
Query: 660 KGK-KLQLPA 686
G+ L+LP+
Sbjct: 261 GGQLVLRLPS 270
>At4g09360.1 68417.m01545 disease resistance protein (NBS-LRR
class), putative domain signature NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 853
Score = 29.1 bits (62), Expect = 3.4
Identities = 12/31 (38%), Positives = 22/31 (70%)
Frame = +1
Query: 433 SVAILPQLFLVSKTREAESITSHYLLL*VHI 525
S+ + P L L S+ E E++TSH+L++ +H+
Sbjct: 766 SINLTPNLNLSSEIEEEETVTSHHLVI-IHV 795
>At5g44860.1 68418.m05499 expressed protein strong similarity to
unknown protein (gb AAC79135.1)
Length = 321
Score = 28.7 bits (61), Expect = 4.5
Identities = 14/43 (32%), Positives = 25/43 (58%)
Frame = +1
Query: 49 KHEYIPFAW*SIPPVSYNNIVIKDLEIKIMCRDIRKVSDSVFS 177
K +I F W S+ + YN++ + L + I+ D++ V +VFS
Sbjct: 127 KRLFITFLWVSLMMLVYNSVFLLFLVVLIVAIDLQSVILAVFS 169
>At2g46970.1 68415.m05867 basic helix-loop-helix (bHLH) protein,
putative similar to PIF3 like basic Helix Loop Helix
protein (PIL1) [Arabidopsis thaliana] GI:22535492;
contains Myc-type, 'helix-loop-helix' dimerization
domain signature, PROSITE:PS00038
Length = 416
Score = 27.9 bits (59), Expect = 7.8
Identities = 22/77 (28%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
Frame = -1
Query: 696 KKLKLATVVSFPSTLS*CKGKRNHST--ELSAQHPR*SLSTHSETPQHNGKPSSRGTELY 523
KKLK ++ + F +S ST ++SA+ P+ ++ + P R TELY
Sbjct: 109 KKLK-SSKIEFERNVSKSNKCVESSTLIDVSAKGPK-NVEVTTAPPDEQSAAVGRSTELY 166
Query: 522 MNLKQQIMRGNAFGLSC 472
+ RG + LSC
Sbjct: 167 FASSSKFSRGTSRDLSC 183
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,421,235
Number of Sequences: 28952
Number of extensions: 348473
Number of successful extensions: 719
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 684
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 711
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1702303248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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