Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30192
         (738 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit...    38   0.001
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po...    31   0.17 
SPCC1183.03c |||frataxin homolog|Schizosaccharomyces pombe|chr 3...    29   0.52 
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces...    29   0.91 
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ...    27   2.1  
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch...    27   2.8  
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb...    27   3.7  
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ...    26   4.9  
SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c...    26   6.4  
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta...    26   6.4  
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    26   6.4  
SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor Raf2|S...    25   8.5  
SPAC11G7.06c |mug132||S. pombe specific UPF0300 family protein 3...    25   8.5  

>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 21/71 (29%), Positives = 41/71 (57%)
 Frame = +2

Query: 275 QARVWQWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTF 454
           +A+V Q+ S+++SEL  A    + P +    +++Q  + A++ +       D  L ++T+
Sbjct: 90  KAKVLQYCSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTY 149

Query: 455 LVTERITLADV 487
           LV  R+TLAD+
Sbjct: 150 LVGSRLTLADI 160



 Score = 31.9 bits (69), Expect = 0.098
 Identities = 17/39 (43%), Positives = 23/39 (58%)
 Frame = +3

Query: 129 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN 245
           N   D   KFP  K+P F   DG   L+E+ AIA+Y+A+
Sbjct: 38  NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLAS 75


>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 220

 Score = 31.1 bits (67), Expect = 0.17
 Identities = 25/76 (32%), Positives = 32/76 (42%)
 Frame = +3

Query: 9   MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 188
           M  G LY++  N R    L  A+     V +   +       S D   KFP  K+P F  
Sbjct: 1   MFLGTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIG 57

Query: 189 ADGKVLLTESNAIAYY 236
           ADG   L+E  AI  Y
Sbjct: 58  ADG-FELSEVIAIVKY 72



 Score = 30.7 bits (66), Expect = 0.23
 Identities = 17/73 (23%), Positives = 42/73 (57%), Gaps = 2/73 (2%)
 Frame = +2

Query: 275 QARVWQWASWSDSELL-PASCA-WVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTR 448
           +A + +W  + + +++ P +   WV  + G + + ++  + + +  + +LK+ +  +  R
Sbjct: 93  EAEMLKWMCFINFDIVTPQNVRPWVGMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDR 152

Query: 449 TFLVTERITLADV 487
           T+LV +R TLAD+
Sbjct: 153 TYLVGDRFTLADL 165


>SPCC1183.03c |||frataxin homolog|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 158

 Score = 29.5 bits (63), Expect = 0.52
 Identities = 13/38 (34%), Positives = 19/38 (50%)
 Frame = +3

Query: 189 ADGKVLLTESNAIAYYVANESLAEEIWLPKPVSGSGHH 302
           A+G + L       Y +  +  A +IWL  PVSG  H+
Sbjct: 79  ANGVITLMLGEKGTYVINKQPPAHQIWLSSPVSGPKHY 116


>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 442

 Score = 28.7 bits (61), Expect = 0.91
 Identities = 20/58 (34%), Positives = 24/58 (41%), Gaps = 2/58 (3%)
 Frame = +3

Query: 402 TYWPP*KYWTDIFSHAPSLLPRESHLPMSLSSVHC*CFPARARP--ERPFVADKRSAL 569
           TY PP  Y  D+FS APS+   +     S+S       PA        PFV    S L
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMSPTPEVVSPAATNSAISSPFVRKSSSDL 314


>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 267

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 13/39 (33%), Positives = 19/39 (48%), Gaps = 1/39 (2%)
 Frame = +3

Query: 246 ESLAEEIWLPKPVSGSGHHGLTANYCL-LPALGSSLTLV 359
           +  +  +W+P PV  SG    T   C+ LP  G  +T V
Sbjct: 17  QPFSNPLWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55


>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
           Eme1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 738

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 16/62 (25%), Positives = 30/62 (48%)
 Frame = +3

Query: 36  PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 215
           P  F++ KA +  QY+          +FG  +K ++F K+    ++  F S +  +L+  
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611

Query: 216 SN 221
           SN
Sbjct: 612 SN 613


>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1073

 Score = 26.6 bits (56), Expect = 3.7
 Identities = 16/68 (23%), Positives = 28/68 (41%), Gaps = 1/68 (1%)
 Frame = +3

Query: 210 TESNAIAYYVANESLAEEIWLP-KPVSGSGHHGLTANYCLLPALGSSLTLVSCNSTNRML 386
           +ES  I YY  N     E+WLP +  S   +   T    L+    ++L +  C+     +
Sbjct: 722 SESRIIVYYSNNTLYLSELWLPQRTFSSKLNLAATPKRLLVDKYTNTLIIGCCHVLVNEI 781

Query: 387 NVQSLTYW 410
               L ++
Sbjct: 782 TTSGLAFY 789


>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 369

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = -3

Query: 205 STFPSALSNAGTFPAGNFFKKSSDL 131
           +TFP A +  GTFP G  F   S L
Sbjct: 52  ATFPWAQAPVGTFPIGQLFDPVSGL 76


>SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 339

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
 Frame = +3

Query: 294 GHHGLTANYCLLPALGSSLTLV--SCNSTN-RMLNVQSL 401
           GH  L  + C  P++GSS  L+  +C   N R+  V++L
Sbjct: 213 GHTDLIRDICWAPSMGSSYYLIATACKDGNVRIFKVETL 251


>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 450

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 13/53 (24%), Positives = 25/53 (47%)
 Frame = +1

Query: 550 LINVQRWFLTVAHQPQVSAVVGSLTLLCGSLLHTTLKSTRS*LVHRTRRRAKK 708
           L NV RWF  + HQ  V     S+++   ++     K  R  ++ + ++  K+
Sbjct: 170 LNNVCRWFDFIQHQESVMEAANSMSMKLANIDLNAPKIQRPSVIKKDKKEKKE 222


>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 673

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 10/20 (50%), Positives = 15/20 (75%)
 Frame = +3

Query: 654 PKKYQELAGAQNKKEGKKRL 713
           P+K Q+LA ++ K + KKRL
Sbjct: 64  PRKNQQLASSERKTKNKKRL 83


>SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor
           Raf2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 636

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 25/90 (27%), Positives = 38/90 (42%), Gaps = 2/90 (2%)
 Frame = +3

Query: 15  AGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPA--GKVPAFES 188
           +G  YTY +    Y    A  +S T+     NFV        DF+K F    G  PAF  
Sbjct: 430 SGSDYTYEQ----YINHSAVAFSFTEEIFEKNFV----TVLPDFVKLFSISFGYWPAFSF 481

Query: 189 ADGKVLLTESNAIAYYVANESLAEEIWLPK 278
            D  + L  +     Y +  +L +++WL +
Sbjct: 482 YDELLKLLRNKYPKVYSSTPNLCDQVWLDR 511


>SPAC11G7.06c |mug132||S. pombe specific UPF0300 family protein
           3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 8/27 (29%), Positives = 19/27 (70%)
 Frame = -1

Query: 249 FHWQRSKRWHCFQLGAPFHRHFRMQAL 169
           FH   ++ +HC+++G P ++++  +AL
Sbjct: 363 FHHDYTE-YHCYRIGKPLYQNYEEEAL 388


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,073,454
Number of Sequences: 5004
Number of extensions: 64987
Number of successful extensions: 216
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)