Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30190
(706 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 133 2e-32
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 89 8e-19
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 87 2e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 69 7e-13
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 31 0.12
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 29 0.65
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 29 0.65
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 28 1.1
SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 28 1.5
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 28 1.5
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.0
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.6
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.5
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 27 3.5
SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|... 26 4.6
SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces... 26 4.6
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 4.6
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 25 8.0
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 8.0
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 8.0
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 133 bits (322), Expect = 2e-32
Identities = 57/73 (78%), Positives = 66/73 (90%)
Frame = +3
Query: 255 LVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKES 434
LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+
Sbjct: 65 LVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREA 124
Query: 435 ESCDCLQGFQLIH 473
E+CD LQGFQL H
Sbjct: 125 EACDALQGFQLTH 137
Score = 113 bits (271), Expect = 3e-26
Identities = 46/64 (71%), Positives = 55/64 (85%)
Frame = +1
Query: 64 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 243
MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 244 PRAI 255
PRA+
Sbjct: 61 PRAV 64
Score = 83.4 bits (197), Expect = 3e-17
Identities = 38/47 (80%), Positives = 43/47 (91%)
Frame = +2
Query: 503 GHLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVE 643
G LL+SKIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVE
Sbjct: 148 GTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVE 194
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 88.6 bits (210), Expect = 8e-19
Identities = 37/77 (48%), Positives = 55/77 (71%), Gaps = 1/77 (1%)
Frame = +3
Query: 246 PRHL-VDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 422
PR + VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 423 RKESESCDCLQGFQLIH 473
R+ +++C LQGF + H
Sbjct: 127 RRMADNCSGLQGFLVFH 143
Score = 50.4 bits (115), Expect = 2e-07
Identities = 25/70 (35%), Positives = 37/70 (52%), Gaps = 6/70 (8%)
Frame = +1
Query: 64 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 225
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 226 SGGKYVPRAI 255
GK+VPR+I
Sbjct: 61 GQGKFVPRSI 70
Score = 44.8 bits (101), Expect = 1e-05
Identities = 19/47 (40%), Positives = 31/47 (65%)
Frame = +2
Query: 503 GHLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVE 643
G LL+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++
Sbjct: 154 GALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLD 200
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 87.4 bits (207), Expect = 2e-18
Identities = 37/77 (48%), Positives = 53/77 (68%), Gaps = 1/77 (1%)
Frame = +3
Query: 246 PRHL-VDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 422
PR + VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +
Sbjct: 63 PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKI 122
Query: 423 RKESESCDCLQGFQLIH 473
R+ +++C LQGF + H
Sbjct: 123 RRIADNCSGLQGFLVFH 139
Score = 56.4 bits (130), Expect = 4e-09
Identities = 27/66 (40%), Positives = 38/66 (57%), Gaps = 2/66 (3%)
Frame = +1
Query: 64 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 237
MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 238 YVPRAI 255
YVPR+I
Sbjct: 61 YVPRSI 66
Score = 46.4 bits (105), Expect = 4e-06
Identities = 19/48 (39%), Positives = 32/48 (66%)
Frame = +2
Query: 500 YGHLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVE 643
+G LL+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++
Sbjct: 149 FGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLD 196
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 68.9 bits (161), Expect = 7e-13
Identities = 29/63 (46%), Positives = 44/63 (69%)
Frame = +1
Query: 67 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 246
REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 247 RAI 255
RAI
Sbjct: 63 RAI 65
Score = 67.3 bits (157), Expect = 2e-12
Identities = 26/75 (34%), Positives = 50/75 (66%), Gaps = 2/75 (2%)
Frame = +3
Query: 255 LVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRK 428
L+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +
Sbjct: 66 LIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDR 124
Query: 429 ESESCDCLQGFQLIH 473
E++ D L+GF L+H
Sbjct: 125 EADGSDSLEGFSLLH 139
Score = 48.0 bits (109), Expect = 1e-06
Identities = 21/46 (45%), Positives = 32/46 (69%), Gaps = 1/46 (2%)
Frame = +2
Query: 503 GHLLISKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQL 637
G L+ ++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L
Sbjct: 150 GSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRL 195
>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1131
Score = 31.5 bits (68), Expect = 0.12
Identities = 21/79 (26%), Positives = 39/79 (49%), Gaps = 3/79 (3%)
Frame = +1
Query: 238 YVPRAISSTWSPAPWTLSARDLSDRSSVRTTSF---SDSPAPATTGPRDTTQRVLSSLTR 408
Y+P A SS ++ + LS+ LS +S +TS+ + S + D + +SS+T
Sbjct: 548 YIPNASSSVYASSTEALSSNSLSSSTSSASTSYIPSASSSYEVASNSSDYYSQTVSSITA 607
Query: 409 SSM*SAKNQNLAIAYRASN 465
S S+ ++ ++ SN
Sbjct: 608 SGTTSSTSEIVSTPASNSN 626
Score = 26.6 bits (56), Expect = 3.5
Identities = 14/41 (34%), Positives = 23/41 (56%)
Frame = +1
Query: 304 SDRSSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SA 426
S SS T+ S + + T P ++ V+SS++ SSM S+
Sbjct: 342 SSSSSASATATSSAESSIATSPITSSSNVVSSISTSSMDSS 382
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 29.1 bits (62), Expect = 0.65
Identities = 31/104 (29%), Positives = 44/104 (42%), Gaps = 5/104 (4%)
Frame = +1
Query: 181 SDLQLERINVYYNEASGGKYVPRAISSTWSPAPWTLSARDLSDRSSVRTTSFSDSPAPAT 360
SDL ++ I +ASG KY +ST P + D S ++ T + +PAP+T
Sbjct: 121 SDLNMDDIIRRVADASGSKYSVHTSNST--PQSKHNAFYDAS-QTFGSTAKVAPAPAPST 177
Query: 361 TGP-----RDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLYIP 477
P + Q S S S+K N A SNL +P
Sbjct: 178 KTPLANISKPVVQAQKDSKDNSWDDSSKQSNTQTANTTSNLRVP 221
>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 709
Score = 29.1 bits (62), Expect = 0.65
Identities = 33/100 (33%), Positives = 45/100 (45%)
Frame = +1
Query: 211 YYNEASGGKYVPRAISSTWSPAPWTLSARDLSDRSSVRTTSFSDSPAPATTGPRDTTQRV 390
YY EA K R++S+ S LS SS T+SFSD A + P+ T+
Sbjct: 107 YYQEALRKKSWSRSLSNNIS-----LS----QSSSSPATSSFSDPKAFSAGVPKFTSD-- 155
Query: 391 LSSLTRSSM*SAKNQNLAIAYRASNLYIPSVAAPGPVWAP 510
T S++ S + N ++ N PS P PVWAP
Sbjct: 156 ----TSSTVSSTPSLNHSL----QNSMPPSTPTPPPVWAP 187
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 28.3 bits (60), Expect = 1.1
Identities = 17/59 (28%), Positives = 32/59 (54%)
Frame = +1
Query: 289 SARDLSDRSSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASN 465
S+ S S++ ++S S S P+T+ +T LSS + SS S+ + + +I +S+
Sbjct: 220 SSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSS 278
>SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 682
Score = 27.9 bits (59), Expect = 1.5
Identities = 29/114 (25%), Positives = 47/114 (41%)
Frame = +1
Query: 244 PRAISSTWSPAPWTLSARDLSDRSSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*S 423
P A S +P+P S + L + + +D PA P+ + S L+
Sbjct: 553 PSAHDSASAPSPHMQSQQALPYQYYNPLPAMAD---PAQNVPQQLPPPIHSHLSDDQHIQ 609
Query: 424 AKNQNLAIAYRASNLYIPSVAAPGPVWAPPHLKDP*RVPRQNHEHILSSPLAQS 585
N + N++ PS A +++P +P+QNHEH SPL +S
Sbjct: 610 YSYPNTFVNRFPQNIHHPSANLLDASAALNPVQNPLLMPQQNHEH---SPLVRS 660
>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 27.9 bits (59), Expect = 1.5
Identities = 20/55 (36%), Positives = 31/55 (56%), Gaps = 8/55 (14%)
Frame = +2
Query: 497 RYGHLLISKIREEYP-DRIMNTYSVVP--SPKVSDTVVE---PYN--ATLSVHQL 637
+Y H++ SK++ EYP D+++ SV+ PK VVE P N A L+ H +
Sbjct: 392 QYAHVVASKLQREYPRDKVLYYSSVLTEFDPKGIQEVVESLRPNNFFAILAAHSI 446
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +3
Query: 366 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 455
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.6
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 103 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 204
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.5
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 297 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 398
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 26.6 bits (56), Expect = 3.5
Identities = 27/94 (28%), Positives = 39/94 (41%), Gaps = 3/94 (3%)
Frame = +1
Query: 256 SSTWSPAPWTLSARDLSDRSSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SA-KN 432
SST SPAPW A+ S + + AP + L + + SA +
Sbjct: 418 SSTSSPAPW---AKPASSAAPSNPAPWQQPAAPQSAPALSMNPSSLPPWQQPTQQSAVQP 474
Query: 433 QNLAIAYRASNLYIPSVAAPGP--VWAPPHLKDP 528
NL + A +IP +AP P +APP + P
Sbjct: 475 SNLVPSQNAP--FIPGTSAPLPPTTFAPPGVPLP 506
>SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 497
Score = 26.2 bits (55), Expect = 4.6
Identities = 24/88 (27%), Positives = 39/88 (44%), Gaps = 3/88 (3%)
Frame = +1
Query: 244 PRAISSTWSPAPWTLSARDLSDRSSV---RTTSFSDSPAPATTGPRDTTQRVLSSLTRSS 414
PR ++ SPAP D+ + R + A +TTG + + LS + + +
Sbjct: 311 PRVANTVVSPAPIPTIGSATIDKDAEGFQRVLAMLGRQASSTTG---SPKVALSQMPQVN 367
Query: 415 M*SAKNQNLAIAYRASNLYIPSVAAPGP 498
+ + NQ+LA + S PS AP P
Sbjct: 368 V-NPSNQDLASVKQPSGFSPPSAVAPPP 394
>SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 967
Score = 26.2 bits (55), Expect = 4.6
Identities = 16/43 (37%), Positives = 21/43 (48%)
Frame = +3
Query: 231 RQVRAPRHLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGN 359
R+V AP+H GT RS +FR ++ FGQ GN
Sbjct: 486 REVFAPKH-ARRRLGTRFHSRSSHRPSVFRDNSVAFGQLDNGN 527
>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 363
Score = 26.2 bits (55), Expect = 4.6
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +2
Query: 47 KITYTK*GKSFIYRPANVV 103
+I+YT G SFIYRP N++
Sbjct: 64 EISYT--GTSFIYRPGNII 80
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 25.4 bits (53), Expect = 8.0
Identities = 19/86 (22%), Positives = 35/86 (40%)
Frame = +1
Query: 157 PTGAYHGDSDLQLERINVYYNEASGGKYVPRAISSTWSPAPWTLSARDLSDRSSVRTTSF 336
P G +G ++ V Y YVP+ + P+T + ++S S +
Sbjct: 391 PYGQVYGQPQYIVDPNMVQYGPILQPGYVPQYYP-VYHQTPYTQNFPNMSRSGSQVSDQV 449
Query: 337 SDSPAPATTGPRDTTQRVLSSLTRSS 414
+SP +T PR+ ++ +SS
Sbjct: 450 VESPNSSTLSPRNGFAPIVKQQKKSS 475
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 8.0
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +3
Query: 318 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 449
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 8.0
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -1
Query: 355 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 260
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,880,527
Number of Sequences: 5004
Number of extensions: 59780
Number of successful extensions: 212
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 207
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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