BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30176 (505 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g02830.1 68414.m00243 60S ribosomal protein L22 (RPL22A) simi... 51 5e-07 At5g27770.1 68418.m03330 60S ribosomal protein L22 (RPL22C) ribo... 49 2e-06 At3g05560.2 68416.m00614 60S ribosomal protein L22-2 (RPL22B) id... 48 4e-06 At3g05560.1 68416.m00613 60S ribosomal protein L22-2 (RPL22B) id... 48 4e-06 At3g60970.1 68416.m06823 ABC transporter family protein ABC tran... 27 5.4 At2g01710.1 68415.m00099 DNAJ heat shock N-terminal domain-conta... 27 5.4 At1g04120.1 68414.m00401 ABC transporter family protein Strong s... 27 5.4 At3g56700.1 68416.m06307 male sterility protein, putative simila... 27 7.2 At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-contain... 27 9.5 At2g12100.1 68415.m01300 Ulp1 protease family protein contains P... 27 9.5 At1g45090.1 68414.m05169 Ulp1 protease family protein similar to... 27 9.5 >At1g02830.1 68414.m00243 60S ribosomal protein L22 (RPL22A) similar to ribosomal protein L22 GI:710294 from [Rattus norvegicus] Length = 127 Score = 50.8 bits (116), Expect = 5e-07 Identities = 20/43 (46%), Positives = 29/43 (67%) Frame = +3 Query: 129 LKFTIDCTHPAEDSILDVGNFEKYLKEHVKVEGKTNNLSNHVS 257 + F IDC+ P +D+IL++ EK+L+E +KV GK L N VS Sbjct: 18 VSFVIDCSKPVDDTILEIATLEKFLQERIKVRGKAGALGNSVS 60 Score = 31.1 bits (67), Expect = 0.44 Identities = 18/55 (32%), Positives = 24/55 (43%), Gaps = 2/55 (3%) Frame = +2 Query: 257 VARDKTKVAITADIPFSXXXXXXXXXXXXXXXXXXDWLRVVASAHD--AYELRYF 415 + R K+ + A+ FS DWLRV+AS D YE+RYF Sbjct: 61 ITRYNGKINVNANSNFSKRYLKYLTKKYLKKYNLRDWLRVIASNKDKNVYEVRYF 115 >At5g27770.1 68418.m03330 60S ribosomal protein L22 (RPL22C) ribosomal protein L22 (cytosolic), Rattus norvegicus, PIR:S52084 Length = 124 Score = 49.2 bits (112), Expect = 2e-06 Identities = 19/43 (44%), Positives = 30/43 (69%) Frame = +3 Query: 129 LKFTIDCTHPAEDSILDVGNFEKYLKEHVKVEGKTNNLSNHVS 257 + FTIDC+ P +D I+++ + EK+L+E +KV GK L + VS Sbjct: 16 VSFTIDCSKPVDDKIMEIASLEKFLQERIKVGGKAGALGDSVS 58 Score = 40.3 bits (90), Expect = 7e-04 Identities = 21/55 (38%), Positives = 28/55 (50%), Gaps = 2/55 (3%) Frame = +2 Query: 257 VARDKTKVAITADIPFSXXXXXXXXXXXXXXXXXXDWLRVVASAHD--AYELRYF 415 + R+K+K+ +TAD FS DWLRV+A+ D YELRYF Sbjct: 59 ITREKSKITVTADGQFSKRYLKYLTKKYLKKHNVRDWLRVIAANKDRNLYELRYF 113 >At3g05560.2 68416.m00614 60S ribosomal protein L22-2 (RPL22B) identical to 60S ribosomal protein L22-2 SP:Q9M9W1 from [Arabidopsis thaliana] Length = 124 Score = 48.0 bits (109), Expect = 4e-06 Identities = 18/43 (41%), Positives = 30/43 (69%) Frame = +3 Query: 129 LKFTIDCTHPAEDSILDVGNFEKYLKEHVKVEGKTNNLSNHVS 257 + FTIDC+ P +D I+++ + EK+L+E +KV GK L + V+ Sbjct: 16 VSFTIDCSKPVDDKIMEIASLEKFLQERIKVGGKAGALGDSVT 58 Score = 40.3 bits (90), Expect = 7e-04 Identities = 21/55 (38%), Positives = 28/55 (50%), Gaps = 2/55 (3%) Frame = +2 Query: 257 VARDKTKVAITADIPFSXXXXXXXXXXXXXXXXXXDWLRVVASAHD--AYELRYF 415 + R+K+K+ +TAD FS DWLRV+A+ D YELRYF Sbjct: 59 ITREKSKITVTADGQFSKRYLKYLTKKYLKKHNVRDWLRVIAANKDRNLYELRYF 113 >At3g05560.1 68416.m00613 60S ribosomal protein L22-2 (RPL22B) identical to 60S ribosomal protein L22-2 SP:Q9M9W1 from [Arabidopsis thaliana] Length = 124 Score = 48.0 bits (109), Expect = 4e-06 Identities = 18/43 (41%), Positives = 30/43 (69%) Frame = +3 Query: 129 LKFTIDCTHPAEDSILDVGNFEKYLKEHVKVEGKTNNLSNHVS 257 + FTIDC+ P +D I+++ + EK+L+E +KV GK L + V+ Sbjct: 16 VSFTIDCSKPVDDKIMEIASLEKFLQERIKVGGKAGALGDSVT 58 Score = 40.3 bits (90), Expect = 7e-04 Identities = 21/55 (38%), Positives = 28/55 (50%), Gaps = 2/55 (3%) Frame = +2 Query: 257 VARDKTKVAITADIPFSXXXXXXXXXXXXXXXXXXDWLRVVASAHD--AYELRYF 415 + R+K+K+ +TAD FS DWLRV+A+ D YELRYF Sbjct: 59 ITREKSKITVTADGQFSKRYLKYLTKKYLKKHNVRDWLRVIAANKDRNLYELRYF 113 >At3g60970.1 68416.m06823 ABC transporter family protein ABC transporter-like proteins Length = 1037 Score = 27.5 bits (58), Expect = 5.4 Identities = 12/40 (30%), Positives = 21/40 (52%) Frame = -1 Query: 232 VLPSTLTCSFKYFSKLPTSKILSSAGCVQSIVNFKLIFRL 113 +L S YF PT +IL+ A QS+++ ++ +L Sbjct: 560 MLCSIFRAPMSYFDSTPTGRILNRASTDQSVLDLEMAVKL 599 >At2g01710.1 68415.m00099 DNAJ heat shock N-terminal domain-containing protein simlar to AHM1 [Triticum aestivum] GI:6691467; contains Pfam profile PF00226: DnaJ domain Length = 311 Score = 27.5 bits (58), Expect = 5.4 Identities = 12/36 (33%), Positives = 17/36 (47%) Frame = -1 Query: 136 NFKLIFRLMPLPRILPPFTPFLPVFWCNWAFLATGF 29 N + F +P+ LPP P ++C W F GF Sbjct: 192 NCQRAFHAASIPQ-LPPLIPGKDEYYCCWGFFPMGF 226 >At1g04120.1 68414.m00401 ABC transporter family protein Strong similarity to MRP-like ABC transporter gb|U92650 from A. thaliana and canalicular multi-drug resistance protein gb|L49379 from Rattus norvegicus Length = 1514 Score = 27.5 bits (58), Expect = 5.4 Identities = 14/44 (31%), Positives = 21/44 (47%) Frame = -1 Query: 244 LRLFVLPSTLTCSFKYFSKLPTSKILSSAGCVQSIVNFKLIFRL 113 L L +L S +F P +IL+ QS+V+ + FRL Sbjct: 1022 LFLNMLRSVFRAPMSFFDSTPAGRILNRVSIDQSVVDLDIPFRL 1065 >At3g56700.1 68416.m06307 male sterility protein, putative similar to SP|Q08891 Male sterility protein 2 {Arabidopsis thaliana}; contains Pfam profile PF03015: Male sterility protein Length = 527 Score = 27.1 bits (57), Expect = 7.2 Identities = 12/36 (33%), Positives = 23/36 (63%) Frame = +3 Query: 147 CTHPAEDSILDVGNFEKYLKEHVKVEGKTNNLSNHV 254 C P E++++D+ +++K H +EG T+ LSN + Sbjct: 402 CDFPLEETVIDL----EHMKIHSSLEGFTSALSNTI 433 >At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to glucocorticoid receptor DNA binding factor 1 [Canis familiaris] GI:23266717; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 827 Score = 26.6 bits (56), Expect = 9.5 Identities = 12/34 (35%), Positives = 19/34 (55%), Gaps = 1/34 (2%) Frame = -1 Query: 433 AVSVDVEVAQFVSVMS-GGHHSKPVTQIVLLEVT 335 A S+ + + ++ VM G HH P T L+E+T Sbjct: 754 AASLGIPMEEYSPVMDYGRHHHPPATSAALMELT 787 >At2g12100.1 68415.m01300 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain; similar to At5g28270, At2g05450, At1g45090, At2g16180, At2g06750 Length = 1224 Score = 26.6 bits (56), Expect = 9.5 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +3 Query: 150 THPAEDSILDVGNFEKYLKEHVKVEGKTNNLSNHVSS 260 +H E I D GNFE+ L + + + +S+ VSS Sbjct: 686 SHQPEHGIPDGGNFEQLLPDPILSDPALEKMSDDVSS 722 >At1g45090.1 68414.m05169 Ulp1 protease family protein similar to At5g28270, At2g12100, At2g05450, At2g16180, At2g06750; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 1210 Score = 26.6 bits (56), Expect = 9.5 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +3 Query: 150 THPAEDSILDVGNFEKYLKEHVKVEGKTNNLSNHVSS 260 +H E I D GNFE+ L + + + +S+ VSS Sbjct: 677 SHQPEHGIPDGGNFEQLLPDPILSDPALEKMSDDVSS 713 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,944,218 Number of Sequences: 28952 Number of extensions: 158457 Number of successful extensions: 446 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 433 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 443 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 898188928 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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