Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30168
(648 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 154 1e-38
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 152 4e-38
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 73 3e-14
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 55 1e-08
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 34 0.020
SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Sc... 28 1.3
SPAC824.02 |||GPI inositol deacylase|Schizosaccharomyces pombe|c... 27 3.1
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 4.1
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 25 7.1
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 7.1
SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster ... 25 7.1
SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|ch... 25 7.1
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 25 9.4
SPAC8C9.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 25 9.4
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 154 bits (373), Expect = 1e-38
Identities = 73/112 (65%), Positives = 85/112 (75%)
Frame = +2
Query: 254 HEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRI 433
+E+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRI
Sbjct: 206 NESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRI 265
Query: 434 HFPLVTYAPVISAEKAYHEQLSVAESQTHASSPPTRW*NATPVMASTWLAVC 589
HFPLVTYAP++SA KA+HE SV E P + P A ++A C
Sbjct: 266 HFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP-RAGRYMATC 316
Score = 120 bits (288), Expect = 2e-28
Identities = 52/84 (61%), Positives = 67/84 (79%)
Frame = +3
Query: 3 IRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAV 182
IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+V
Sbjct: 122 IRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSV 181
Query: 183 VEPYNSILTTHTTLEHSDCAFMVD 254
VEPYNS+LTTH TL+ +DC FMVD
Sbjct: 182 VEPYNSVLTTHATLDLADCTFMVD 205
Score = 79.8 bits (188), Expect = 3e-16
Identities = 31/44 (70%), Positives = 38/44 (86%)
Frame = +1
Query: 502 RRVTNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 633
+ +TN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+
Sbjct: 289 QEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAV 332
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 152 bits (369), Expect = 4e-38
Identities = 73/112 (65%), Positives = 83/112 (74%)
Frame = +2
Query: 254 HEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRI 433
+EA YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLVPYPRI
Sbjct: 210 NEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRI 269
Query: 434 HFPLVTYAPVISAEKAYHEQLSVAESQTHASSPPTRW*NATPVMASTWLAVC 589
HFPLVTY+P++SA KA+HE SV E P + P ++A C
Sbjct: 270 HFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP-RTGRYMATC 320
Score = 124 bits (298), Expect = 1e-29
Identities = 53/84 (63%), Positives = 68/84 (80%)
Frame = +3
Query: 3 IRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAV 182
IR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAPQVST+V
Sbjct: 126 IRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSV 185
Query: 183 VEPYNSILTTHTTLEHSDCAFMVD 254
VEPYNS+LTTH TL++SDC FMVD
Sbjct: 186 VEPYNSVLTTHATLDNSDCTFMVD 209
Score = 79.8 bits (188), Expect = 3e-16
Identities = 31/44 (70%), Positives = 38/44 (86%)
Frame = +1
Query: 502 RRVTNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 633
+ +TN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+
Sbjct: 293 QEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAV 336
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 73.3 bits (172), Expect = 3e-14
Identities = 32/85 (37%), Positives = 53/85 (62%)
Frame = +2
Query: 254 HEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRI 433
+EA+ I L I+ P+Y +LN L+ +++ +T S RF G LN DL + N+VP+PR+
Sbjct: 204 NEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRL 263
Query: 434 HFPLVTYAPVISAEKAYHEQLSVAE 508
HF +V +AP+ + + + +SV E
Sbjct: 264 HFFMVGFAPLAAIGSSSFQAVSVPE 288
Score = 63.7 bits (148), Expect = 2e-11
Identities = 29/84 (34%), Positives = 46/84 (54%)
Frame = +3
Query: 3 IRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAV 182
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S V
Sbjct: 120 VRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTV 179
Query: 183 VEPYNSILTTHTTLEHSDCAFMVD 254
VEPYN+ L+ H +E+SD F +D
Sbjct: 180 VEPYNATLSMHQLVENSDETFCID 203
Score = 42.3 bits (95), Expect = 6e-05
Identities = 18/42 (42%), Positives = 25/42 (59%)
Frame = +1
Query: 508 VTNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 633
+T F+ N MV DPRHG+Y+ L+RG V K+V+ I
Sbjct: 289 LTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQI 330
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 54.8 bits (126), Expect = 1e-08
Identities = 24/67 (35%), Positives = 38/67 (56%)
Frame = +2
Query: 260 AIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHF 439
A+ I L + PT+ N+L+ ++S+ T +LR+ G +N DL +L+P PR HF
Sbjct: 209 ALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHF 268
Query: 440 PLVTYAP 460
L +Y P
Sbjct: 269 LLTSYTP 275
Score = 46.4 bits (105), Expect = 4e-06
Identities = 27/85 (31%), Positives = 42/85 (49%), Gaps = 1/85 (1%)
Frame = +3
Query: 3 IRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTA 179
I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181
Query: 180 VVEPYNSILTTHTTLEHSDCAFMVD 254
VV+PYNS+L ++D ++D
Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLD 206
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 33.9 bits (74), Expect = 0.020
Identities = 18/54 (33%), Positives = 30/54 (55%)
Frame = -3
Query: 421 RHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 260
R + E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC428.07 |meu6||meiotic chromosome segregation protein
Meu6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 651
Score = 27.9 bits (59), Expect = 1.3
Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = +2
Query: 377 ALNVDLTEFQTNLVPYPRIHFPLVT--YAPVISAEKAYHEQLSVAESQTHASSPPTRW*N 550
A +V++ E +TN +P HFP T AP S EK + S + T+ N
Sbjct: 368 AQSVEVPENETNQIPTTEEHFPATTEEVAPA-SEEKPATGPAEESTSTQNVEQASTQNDN 426
Query: 551 ATPV 562
TP+
Sbjct: 427 GTPI 430
>SPAC824.02 |||GPI inositol deacylase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1142
Score = 26.6 bits (56), Expect = 3.1
Identities = 13/57 (22%), Positives = 26/57 (45%)
Frame = -1
Query: 573 VLAMTGVAFHHLVGGLEACVCDSATESCSW*AFSAEMTGAYVTSGKWIRG*GTKLVW 403
++++ +AF +L+ +E V D A SW F+ + ++ + G T W
Sbjct: 788 LVSILSIAFSYLISRVELIVADGADPVASWKIFAMMVPKSFWKQNHLLFGLQTAQFW 844
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 26.2 bits (55), Expect = 4.1
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = -3
Query: 637 KRWPRSHPWVRRHHGTAYSKPCTCHDGG 554
K+W R HP+ Y+KPC DGG
Sbjct: 14 KQWRRDHPF------GFYAKPCKSSDGG 35
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 25.4 bits (53), Expect = 7.1
Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 5/48 (10%)
Frame = -2
Query: 308 RLGAQCRDYGGRCHR-----WLHVDHESTVRVLKGCVGGEDRVVGLDD 180
RL QCR + + WL + HES +++L VGG+ + +DD
Sbjct: 877 RLSRQCRAFTNGFTQIIDPHWLAMFHESEIQIL---VGGDPVPIDIDD 921
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 25.4 bits (53), Expect = 7.1
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = -1
Query: 636 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGG 529
S G +LGY ++ A++V+A + V HL+ G
Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDG 442
>SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster
type |Schizosaccharomyces pombe|chr 2|||Manual
Length = 827
Score = 25.4 bits (53), Expect = 7.1
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +1
Query: 499 RRRVTNACFEPANQMVKCDPR 561
R+RV+ AC E + +KCD R
Sbjct: 9 RQRVSRACDECHRRKIKCDQR 29
>SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 815
Score = 25.4 bits (53), Expect = 7.1
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = +1
Query: 475 EGLP*TAFRRRVTNACFEPANQMVKCD 555
EG RRRVT AC + +KCD
Sbjct: 16 EGAKVATRRRRVTRACDMCRRKKIKCD 42
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 25.0 bits (52), Expect = 9.4
Identities = 16/50 (32%), Positives = 20/50 (40%)
Frame = +2
Query: 227 AL*LCFHGRHEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDG 376
AL CF A YDI +R E L R++ I+ A L G
Sbjct: 225 ALNECFKETKNAAYDIIQRKGSTEYGVAAGLVRILAAIIRDENALLTVSG 274
>SPAC8C9.04 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 647
Score = 25.0 bits (52), Expect = 9.4
Identities = 16/58 (27%), Positives = 26/58 (44%)
Frame = +3
Query: 390 TSPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPSHKRMLRARQPDGEMRPPS 563
T+ S R++ + H + SS + PT S P+ S + AR+P + PS
Sbjct: 465 TTASKRVSKHDKASAEKHKVARKPSSTGQEPTTPSTPAKSAQSSKHARRPSKQASAPS 522
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,703,398
Number of Sequences: 5004
Number of extensions: 55168
Number of successful extensions: 197
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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