Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30160
(719 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P22314 Cluster: Ubiquitin-activating enzyme E1; n=101; ... 97 5e-19
UniRef50_P22515 Cluster: Ubiquitin-activating enzyme E1 1; n=80;... 76 1e-12
UniRef50_Q27481 Cluster: Putative uncharacterized protein uba-1;... 65 1e-09
UniRef50_UPI00005A5D6B Cluster: PREDICTED: similar to Ral guanin... 51 3e-05
UniRef50_Q014F3 Cluster: Ubiquitin activating enzyme; n=1; Ostre... 46 7e-04
UniRef50_Q23QY3 Cluster: Ubiquitin-activating enzyme E1 family p... 42 0.015
UniRef50_A0AVT1 Cluster: Ubiquitin-activating enzyme E1-like pro... 42 0.015
UniRef50_A6N189 Cluster: Ubiquitin-activating enzyme e1 2; n=3; ... 40 0.047
UniRef50_Q7RNE0 Cluster: Uba1 gene product-related; n=7; Plasmod... 40 0.062
UniRef50_A7SBV9 Cluster: Predicted protein; n=1; Nematostella ve... 40 0.062
UniRef50_O62844 Cluster: Ubiquitin activating enzyme E1; n=1; Le... 39 0.11
UniRef50_A3BNP3 Cluster: Putative uncharacterized protein; n=1; ... 36 1.3
UniRef50_Q5CR33 Cluster: Ubiquitin-activating enzyme E1; n=2; Cr... 35 1.8
UniRef50_A5APJ0 Cluster: Putative uncharacterized protein; n=2; ... 35 2.3
UniRef50_A6QUE9 Cluster: Ubiquitin-activating enzyme E1 X; n=1; ... 35 2.3
UniRef50_Q03376 Cluster: Balbiani ring protein 3 precursor; n=4;... 34 3.1
UniRef50_Q7QTU0 Cluster: GLP_191_9167_5889; n=1; Giardia lamblia... 33 7.1
UniRef50_Q17166 Cluster: Cuticular collagen Bmcol-2; n=1; Brugia... 33 7.1
>UniRef50_P22314 Cluster: Ubiquitin-activating enzyme E1; n=101;
root|Rep: Ubiquitin-activating enzyme E1 - Homo sapiens
(Human)
Length = 1058
Score = 96.7 bits (230), Expect = 5e-19
Identities = 48/76 (63%), Positives = 57/76 (75%), Gaps = 6/76 (7%)
Frame = -2
Query: 685 LWDRFEVKG------EITLQQFLDHFKNEHKLEITMLSQGVCMLYSFFMPKAKRLERLNL 524
LWDRFEV+G E+TL+QFLD+FK EHKLEITMLSQGV MLYSFFMP AK ERL+
Sbjct: 954 LWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQ 1013
Query: 523 PMSEVSQKCPRRSWNR 476
PM+E+ + +R R
Sbjct: 1014 PMTEIVSRVSKRKLGR 1029
Score = 64.9 bits (151), Expect = 2e-09
Identities = 27/39 (69%), Positives = 33/39 (84%)
Frame = -1
Query: 509 VTKVSKKKLEPHVNALVFELCCNDDDGNDVEVPYVKYTL 393
V++VSK+KL HV ALV ELCCND+ G DVEVPYV+YT+
Sbjct: 1019 VSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTI 1057
>UniRef50_P22515 Cluster: Ubiquitin-activating enzyme E1 1; n=80;
cellular organisms|Rep: Ubiquitin-activating enzyme E1 1
- Saccharomyces cerevisiae (Baker's yeast)
Length = 1024
Score = 75.8 bits (178), Expect = 1e-12
Identities = 31/61 (50%), Positives = 45/61 (73%)
Frame = -2
Query: 691 DHLWDRFEVKGEITLQQFLDHFKNEHKLEITMLSQGVCMLYSFFMPKAKRLERLNLPMSE 512
D +WDRF++KG+I L ++HF+ + LEITMLS GV +LY+ F P K ERLNLP+++
Sbjct: 925 DKIWDRFDIKGDIKLSDLIEHFEKDEGLEITMLSYGVSLLYASFFPPKKLKERLNLPITQ 984
Query: 511 V 509
+
Sbjct: 985 L 985
Score = 42.3 bits (95), Expect = 0.012
Identities = 15/35 (42%), Positives = 25/35 (71%)
Frame = -1
Query: 509 VTKVSKKKLEPHVNALVFELCCNDDDGNDVEVPYV 405
V V+KK + HV+ ++ E+C +D +G DVEVP++
Sbjct: 986 VKLVTKKDIPAHVSTMILEICADDKEGEDVEVPFI 1020
>UniRef50_Q27481 Cluster: Putative uncharacterized protein uba-1; n=3;
Caenorhabditis|Rep: Putative uncharacterized protein
uba-1 - Caenorhabditis elegans
Length = 1113
Score = 65.3 bits (152), Expect = 1e-09
Identities = 29/68 (42%), Positives = 47/68 (69%), Gaps = 1/68 (1%)
Frame = -2
Query: 685 LWDRFEVKGEITLQQFLDHFKNEHK-LEITMLSQGVCMLYSFFMPKAKRLERLNLPMSEV 509
LWDR +V+G +TLQ+F+D+ +N+ E++MLS G C+L+SFFM K+ ERL + V
Sbjct: 1015 LWDRIDVQGPLTLQEFIDNVQNQTGGCEVSMLSAGACLLFSFFMNAGKKQERLKTEVKAV 1074
Query: 508 SQKCPRRS 485
++ ++S
Sbjct: 1075 YEELLKKS 1082
Score = 44.8 bits (101), Expect = 0.002
Identities = 19/33 (57%), Positives = 24/33 (72%)
Frame = -1
Query: 494 KKKLEPHVNALVFELCCNDDDGNDVEVPYVKYT 396
KK L P V+ALV E +D DG DVEVPY++Y+
Sbjct: 1080 KKSLHPSVHALVLEPMMSDPDGEDVEVPYIRYS 1112
>UniRef50_UPI00005A5D6B Cluster: PREDICTED: similar to Ral guanine
nucleotide dissociation stimulator (RalGEF) (RalGDS);
n=1; Canis lupus familiaris|Rep: PREDICTED: similar to
Ral guanine nucleotide dissociation stimulator (RalGEF)
(RalGDS) - Canis familiaris
Length = 324
Score = 50.8 bits (116), Expect = 3e-05
Identities = 24/40 (60%), Positives = 26/40 (65%)
Frame = -1
Query: 509 VTKVSKKKLEPHVNALVFELCCNDDDGNDVEVPYVKYTLP 390
V+ V K+KL HV ALV ELCCND G DVEVP T P
Sbjct: 74 VSHVWKRKLGRHVRALVLELCCNDKSGKDVEVPLYVITWP 113
>UniRef50_Q014F3 Cluster: Ubiquitin activating enzyme; n=1;
Ostreococcus tauri|Rep: Ubiquitin activating enzyme -
Ostreococcus tauri
Length = 879
Score = 46.4 bits (105), Expect = 7e-04
Identities = 22/61 (36%), Positives = 41/61 (67%)
Frame = -2
Query: 685 LWDRFEVKGEITLQQFLDHFKNEHKLEITMLSQGVCMLYSFFMPKAKRLERLNLPMSEVS 506
+WDR+ ++G++T+Q+ +DHF+ + + +M S G ++Y+ PK + ERLN +SE+
Sbjct: 784 MWDRWILEGDLTVQEVIDHFEAKGLIAYSM-SVGASLVYNNIFPKHR--ERLNQKLSELV 840
Query: 505 Q 503
Q
Sbjct: 841 Q 841
>UniRef50_Q23QY3 Cluster: Ubiquitin-activating enzyme E1 family
protein; n=5; Oligohymenophorea|Rep: Ubiquitin-activating
enzyme E1 family protein - Tetrahymena thermophila SB210
Length = 1091
Score = 41.9 bits (94), Expect = 0.015
Identities = 17/49 (34%), Positives = 32/49 (65%)
Frame = -2
Query: 682 WDRFEVKGEITLQQFLDHFKNEHKLEITMLSQGVCMLYSFFMPKAKRLE 536
WD+ V+G +T+Q D+F +++++ I++LS G LY+ +M +A E
Sbjct: 995 WDKLFVQGPLTIQGLKDYFNDKYQVNISILSVGKICLYNSYMTEAAERE 1043
>UniRef50_A0AVT1 Cluster: Ubiquitin-activating enzyme E1-like protein
2; n=28; Euteleostomi|Rep: Ubiquitin-activating enzyme
E1-like protein 2 - Homo sapiens (Human)
Length = 1052
Score = 41.9 bits (94), Expect = 0.015
Identities = 24/53 (45%), Positives = 34/53 (64%), Gaps = 3/53 (5%)
Frame = -2
Query: 685 LWDRFEVKG--EITLQQFLDHFKNEHKLEITMLSQGVCMLYSFFMP-KAKRLE 536
+WDR+ V G + TL F++ K ++ +E TM+ QGV MLY MP AKRL+
Sbjct: 954 IWDRWTVHGKEDFTLLDFINAVKEKYGIEPTMVVQGVKMLYVPVMPGHAKRLK 1006
>UniRef50_A6N189 Cluster: Ubiquitin-activating enzyme e1 2; n=3;
Oryza sativa|Rep: Ubiquitin-activating enzyme e1 2 -
Oryza sativa subsp. indica (Rice)
Length = 98
Score = 40.3 bits (90), Expect = 0.047
Identities = 22/62 (35%), Positives = 37/62 (59%)
Frame = -2
Query: 685 LWDRFEVKGEITLQQFLDHFKNEHKLEITMLSQGVCMLYSFFMPKAKRLERLNLPMSEVS 506
+WDR+ + G ITL++ LD K E L +S G +LY+ P+ K ERL+ + +V+
Sbjct: 3 VWDRWTITGNITLRELLDWLK-EKGLNAYSISCGTSLLYNSMFPRHK--ERLDKKVVDVA 59
Query: 505 QK 500
++
Sbjct: 60 RE 61
>UniRef50_Q7RNE0 Cluster: Uba1 gene product-related; n=7;
Plasmodium|Rep: Uba1 gene product-related - Plasmodium
yoelii yoelii
Length = 1176
Score = 39.9 bits (89), Expect = 0.062
Identities = 16/56 (28%), Positives = 34/56 (60%)
Frame = -2
Query: 652 TLQQFLDHFKNEHKLEITMLSQGVCMLYSFFMPKAKRLERLNLPMSEVSQKCPRRS 485
T++ +DH + +++ ++S G LY+ ++P A ERLN P+ E+ ++ ++S
Sbjct: 1090 TIKDLVDHINEKFNIDVNLISVGNACLYNCYLP-AHNKERLNKPIHEIYEQISKQS 1144
>UniRef50_A7SBV9 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella vectensis
Length = 1013
Score = 39.9 bits (89), Expect = 0.062
Identities = 22/53 (41%), Positives = 34/53 (64%), Gaps = 3/53 (5%)
Frame = -2
Query: 685 LWDRFEVKGE--ITLQQFLDHFKNEHKLEITMLSQGVCMLYSFFMP-KAKRLE 536
+WDR+ VKG L+ F K+++ L++TM+ QGV M+Y +P AKRL+
Sbjct: 920 IWDRWIVKGNKNFKLKDFNQCIKDQYGLQVTMVVQGVKMIYVPVVPGHAKRLD 972
>UniRef50_O62844 Cluster: Ubiquitin activating enzyme E1; n=1; Lemur
catta|Rep: Ubiquitin activating enzyme E1 - Lemur catta
(Ring-tailed lemur)
Length = 54
Score = 39.1 bits (87), Expect = 0.11
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = -1
Query: 620 RTQVGDHHVVSRCVHALFVLHAQSQAAGT 534
R Q+ DHH + +HALF LHA QA GT
Sbjct: 26 RAQIRDHHAIPGRIHALFFLHATRQAQGT 54
>UniRef50_A3BNP3 Cluster: Putative uncharacterized protein; n=1; Oryza
sativa (japonica cultivar-group)|Rep: Putative
uncharacterized protein - Oryza sativa subsp. japonica
(Rice)
Length = 1270
Score = 35.5 bits (78), Expect = 1.3
Identities = 19/62 (30%), Positives = 36/62 (58%)
Frame = -2
Query: 685 LWDRFEVKGEITLQQFLDHFKNEHKLEITMLSQGVCMLYSFFMPKAKRLERLNLPMSEVS 506
+WDR+ +KG +T+ + L F ++ L +S G +LY+ A+ ERLN + +V+
Sbjct: 1175 VWDRWSIKGNLTVAELLQWF-SDKGLTAYSISCGTSLLYNNMF--ARHKERLNKKVVDVA 1231
Query: 505 QK 500
++
Sbjct: 1232 RE 1233
>UniRef50_Q5CR33 Cluster: Ubiquitin-activating enzyme E1; n=2;
Cryptosporidium|Rep: Ubiquitin-activating enzyme E1 -
Cryptosporidium parvum Iowa II
Length = 1067
Score = 35.1 bits (77), Expect = 1.8
Identities = 14/38 (36%), Positives = 25/38 (65%), Gaps = 1/38 (2%)
Frame = -1
Query: 509 VTKVSKKKLEPHVNALVFEL-CCNDDDGNDVEVPYVKY 399
+ +++KKKL N++ E+ CC+ DDG D +P +K+
Sbjct: 1027 IEQITKKKLHVTKNSIALEVSCCDIDDGVDTIIPSIKF 1064
>UniRef50_A5APJ0 Cluster: Putative uncharacterized protein; n=2; core
eudicotyledons|Rep: Putative uncharacterized protein -
Vitis vinifera (Grape)
Length = 1228
Score = 34.7 bits (76), Expect = 2.3
Identities = 28/92 (30%), Positives = 38/92 (41%), Gaps = 9/92 (9%)
Frame = -1
Query: 641 IFRSLQKRTQVGDHHV----VSRCVHALFVLHAQSQAAGTIEPANVGGVTKVSKKKLEPH 474
+F K +V H + S CV FV ++ Q A + G K KL+
Sbjct: 922 VFHERTKHIEVDCHFIRKKIASGCVATSFV-NSNDQLADIFTKSLRGPKIKYICNKLDAF 980
Query: 473 VNALVFELCCNDDD-----GNDVEVPYVKYTL 393
+AL E CNDDD G+ + YV Y L
Sbjct: 981 CDALSVEKVCNDDDFFMMGGDSIAAAYVSYNL 1012
>UniRef50_A6QUE9 Cluster: Ubiquitin-activating enzyme E1 X; n=1;
Ajellomyces capsulatus NAm1|Rep: Ubiquitin-activating
enzyme E1 X - Ajellomyces capsulatus NAm1
Length = 1219
Score = 34.7 bits (76), Expect = 2.3
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = -1
Query: 509 VTKVSKKKLEPHVNALVFELCCNDDDGNDVEVPYV 405
V VS K + H ++FE DD DV+VPYV
Sbjct: 1169 VQSVSDKAIPDHQKYVIFEFLARDDTDEDVDVPYV 1203
>UniRef50_Q03376 Cluster: Balbiani ring protein 3 precursor; n=4;
Chironomus|Rep: Balbiani ring protein 3 precursor -
Chironomus tentans (Midge)
Length = 1700
Score = 34.3 bits (75), Expect = 3.1
Identities = 18/57 (31%), Positives = 22/57 (38%), Gaps = 5/57 (8%)
Frame = -3
Query: 618 NTSWRSPCCLKVCACFIRSSCPKPSGWND*TCQCR-----RCHKSVQEEVGTACECI 463
N W C C C +CP WN TCQC +C Q AC+C+
Sbjct: 1335 NQQWNEKDC--ECKCSATGNCPAGQTWNSQTCQCSCPATGKC-TGAQVWCSKACKCV 1388
>UniRef50_Q7QTU0 Cluster: GLP_191_9167_5889; n=1; Giardia lamblia ATCC
50803|Rep: GLP_191_9167_5889 - Giardia lamblia ATCC 50803
Length = 1092
Score = 33.1 bits (72), Expect = 7.1
Identities = 14/50 (28%), Positives = 28/50 (56%)
Frame = -2
Query: 682 WDRFEVKGEITLQQFLDHFKNEHKLEITMLSQGVCMLYSFFMPKAKRLER 533
WD E+ +T+Q +D+F++ +K ++ + M+YS F A L++
Sbjct: 1006 WDHIELPKTVTVQGVIDYFRDRYKGDVDSIIFNTRMVYSSFGNGAVALDK 1055
>UniRef50_Q17166 Cluster: Cuticular collagen Bmcol-2; n=1; Brugia
malayi|Rep: Cuticular collagen Bmcol-2 - Brugia malayi
(Filarial nematode worm)
Length = 329
Score = 33.1 bits (72), Expect = 7.1
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = -1
Query: 242 FQIITHSRFVSFVCSLSNDDVLHFYYYFTS*KSFLPNSIFYCKIRWVCMWXE 87
F + S SF+C++S V FY Y +S + N + +CK+R +W E
Sbjct: 27 FYGVALSTIASFICAIS---VPMFYNYLQHVQSVMQNEVDFCKLRSSNIWRE 75
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 614,495,793
Number of Sequences: 1657284
Number of extensions: 11381958
Number of successful extensions: 25538
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 24734
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25529
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 58264468239
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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