Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30160
         (719 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ...    68   2e-12
SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering compo...    29   0.51 
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch...    27   3.6  
SPBPB2B2.02 |mug180||esterase/lipase |Schizosaccharomyces pombe|...    26   4.7  
SPBC11B10.09 |cdc2|swo2|cyclin-dependent protein kinase Cdc2|Sch...    26   6.2  
SPAC1805.14 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   6.2  

>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1012

 Score = 67.7 bits (158), Expect = 2e-12
 Identities = 30/74 (40%), Positives = 51/74 (68%)
 Frame = -2

Query: 709  LHGE*VDHLWDRFEVKGEITLQQFLDHFKNEHKLEITMLSQGVCMLYSFFMPKAKRLERL 530
            ++G+ +D +WDR+ +  + TLQ+ +D+F+ E  LE+TMLS GV +LY+ F P  K  ERL
Sbjct: 908  VNGKEIDKIWDRYNLP-DCTLQELIDYFQKEEGLEVTMLSSGVSLLYANFQPPKKLAERL 966

Query: 529  NLPMSEVSQKCPRR 488
             L +SE+ ++  ++
Sbjct: 967  PLKISELVEQITKK 980



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 19/35 (54%), Positives = 27/35 (77%)
 Frame = -1

Query: 509  VTKVSKKKLEPHVNALVFELCCNDDDGNDVEVPYV 405
            V +++KKKLEP    LV E+CC+D +G DVEVP++
Sbjct: 974  VEQITKKKLEPFRKHLVLEICCDDANGEDVEVPFI 1008


>SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering
           component Pep7 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 536

 Score = 29.5 bits (63), Expect = 0.51
 Identities = 20/52 (38%), Positives = 27/52 (51%), Gaps = 2/52 (3%)
 Frame = -2

Query: 643 QFLDHFKNEHKLEITMLSQGVCMLYSFFMPKAKRLER--LNLPMSEVSQKCP 494
           +FL   K   KLE    S+ V ML +  + KAKRLE+  ++     V Q CP
Sbjct: 231 EFLRLSKRMKKLEELWTSENVSMLDALLLNKAKRLEQSIVHWQDDSVVQICP 282


>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1133

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 18/47 (38%), Positives = 24/47 (51%), Gaps = 2/47 (4%)
 Frame = -1

Query: 254 LQSKFQIITHSRFVSFVCSLSNDDVLHFYY-YFTS*KSFL-PNSIFY 120
           L S+F+    S F  F    S+DD  HFY   +T   + L PNS+ Y
Sbjct: 475 LWSRFRFPKDSEFPEFFKCSSDDDNTHFYVNLYTGETTMLFPNSMPY 521


>SPBPB2B2.02 |mug180||esterase/lipase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 381

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 15/38 (39%), Positives = 20/38 (52%)
 Frame = +1

Query: 397 VYFTYGTSTSFPSSSLQHNSKTNAFTCGSNFFLDTFVT 510
           +YFT GT+  FP   L+HN  +  F C S   L   +T
Sbjct: 23  LYFTIGTN--FPHDELRHNLLSTLF-CSSMLHLSKGLT 57


>SPBC11B10.09 |cdc2|swo2|cyclin-dependent protein kinase
           Cdc2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 297

 Score = 25.8 bits (54), Expect = 6.2
 Identities = 14/34 (41%), Positives = 20/34 (58%)
 Frame = -1

Query: 647 TAIFRSLQKRTQVGDHHVVSRCVHALFVLHAQSQ 546
           TAI R +    +V D +  S CV  L +LHA+S+
Sbjct: 47  TAI-REISLLKEVNDENNRSNCVRLLDILHAESK 79


>SPAC1805.14 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 452

 Score = 25.8 bits (54), Expect = 6.2
 Identities = 10/16 (62%), Positives = 12/16 (75%)
 Frame = +3

Query: 486 LLLGHFCDTSDIGRFN 533
           LL  HFCD S+I +FN
Sbjct: 262 LLDHHFCDLSEISKFN 277


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,624,391
Number of Sequences: 5004
Number of extensions: 51307
Number of successful extensions: 154
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)