Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30143
         (674 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...   103   2e-23
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    56   5e-09
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    54   2e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    51   1e-07
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    48   1e-06
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    33   0.029
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    29   0.46 
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha...    28   1.1  
SPAC56F8.05c |mug64||conserved fungal protein|Schizosaccharomyce...    27   2.5  
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ...    27   3.3  
SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k...    26   5.7  
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy...    26   5.7  
SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog Chs2|Schiz...    26   5.7  
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma...    25   7.6  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score =  103 bits (247), Expect = 2e-23
 Identities = 48/58 (82%), Positives = 53/58 (91%)
 Frame = -3

Query: 174 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNG 1
           LT+EQIAEF+EAFSLFD+D DG IT+ ELG VMRSLGQ+PT AELQDMINEVDADGNG
Sbjct: 6   LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNG 63



 Score = 61.3 bits (142), Expect = 1e-10
 Identities = 30/62 (48%), Positives = 42/62 (67%)
 Frame = -3

Query: 186 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG 7
           M D   EE++   +EAF +FDKDG+G IT +EL  V+ SLG+  ++ E+ DMI E D DG
Sbjct: 78  MKDTDNEEEV---REAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDG 134

Query: 6   NG 1
           +G
Sbjct: 135 DG 136



 Score = 36.3 bits (80), Expect = 0.004
 Identities = 19/53 (35%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
 Frame = -3

Query: 156 AEFKEAFSLFDKDGDGTITTKELGTVM-RSLGQNPTEAELQDMINEVDADGNG 1
           AE ++  +  D DG+GTI   E  T+M R +     E E+++     D DGNG
Sbjct: 48  AELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNG 100


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 56.0 bits (129), Expect = 5e-09
 Identities = 27/51 (52%), Positives = 36/51 (70%)
 Frame = -3

Query: 171 TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEV 19
           ++EQ  E KEAF L+D D DG I T  +G+V+RSLG N T+AEL  + NE+
Sbjct: 4   SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL 54



 Score = 45.2 bits (102), Expect = 9e-06
 Identities = 23/65 (35%), Positives = 39/65 (60%), Gaps = 1/65 (1%)
 Frame = -3

Query: 192 STMADQLTE-EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 16
           S ++++L E E   E+ +AF +FDKD  G I T +    M++LG+  ++ E+Q M+ E D
Sbjct: 65  SFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEAD 124

Query: 15  ADGNG 1
              +G
Sbjct: 125 PTNSG 129


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 54.0 bits (124), Expect = 2e-08
 Identities = 24/54 (44%), Positives = 35/54 (64%)
 Frame = -3

Query: 177 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 16
           QLT  QI E KEAF+L DKDGDG I  +++ T++ SL Q+ +E  +  M   ++
Sbjct: 41  QLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94



 Score = 25.8 bits (54), Expect = 5.7
 Identities = 12/46 (26%), Positives = 20/46 (43%)
 Frame = -3

Query: 144 EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG 7
           EAFS FD    G I    +   + S+G      E++ ++    + G
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSYTSHG 166


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 51.2 bits (117), Expect = 1e-07
 Identities = 24/59 (40%), Positives = 34/59 (57%)
 Frame = -3

Query: 177 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNG 1
           ++TEEQ  +  EAF LFD D D  I   EL   MR+LG N  ++E+  ++ + D  G G
Sbjct: 30  EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKG 88


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 48.0 bits (109), Expect = 1e-06
 Identities = 20/47 (42%), Positives = 33/47 (70%)
 Frame = -3

Query: 150 FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDAD 10
           +K+AFSLFD+ G G I    +G ++R+ GQNPT AE+ ++ + + A+
Sbjct: 8   YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAE 54



 Score = 37.1 bits (82), Expect = 0.002
 Identities = 17/45 (37%), Positives = 26/45 (57%)
 Frame = -3

Query: 153 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEV 19
           EF + F +FDKD  G I   EL  V+ SLG+  +  E+ +++  V
Sbjct: 78  EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV 122


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 33.5 bits (73), Expect = 0.029
 Identities = 19/53 (35%), Positives = 28/53 (52%)
 Frame = -3

Query: 165 EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG 7
           E   E +EAF LFD    G I  ++L      LG+N T+ +LQ M++    +G
Sbjct: 9   EMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNG 61


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 29.5 bits (63), Expect = 0.46
 Identities = 25/80 (31%), Positives = 39/80 (48%), Gaps = 9/80 (11%)
 Frame = +3

Query: 258 PTAET-ILPGGARY---PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPL 410
           P  ET + PGG+     P  P+ S ++ H   +    +    +L N I+L      ++PL
Sbjct: 155 PLPETPVSPGGSLVHPLPRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPL 214

Query: 411 SPAGVIAKRPAPIALPNSCA 470
           SP    A+ P+PI L +S A
Sbjct: 215 SPTRSPARTPSPIRLYSSDA 234


>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
           1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1011

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 15/56 (26%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
 Frame = -3

Query: 180 DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLG--QNPTEAELQDMINEV 19
           D+  E   A  +  +S+FD+D +G IT +E+      +G  +    A L+D+ + +
Sbjct: 538 DETGEVDNATLEACYSIFDRDLNGDITCEEIELACVEIGKERKSISASLRDLNDSI 593


>SPAC56F8.05c |mug64||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 295

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 26/88 (29%), Positives = 35/88 (39%), Gaps = 1/88 (1%)
 Frame = +3

Query: 201 DYLELH*KITDYA-AKATAGPTAETILPGGARYPIRPIVSRITIHWPSFYNVVTGKTLAL 377
           DY+EL  ++     A     P  ET+   G  YP       IT     F   V+GK   L
Sbjct: 55  DYVELEQQVDSLKEAYNLVLPIVETVEVDGYDYPTN-FRDSIT----DFGKTVSGKVRNL 109

Query: 378 PNLIALQHIPLSPAGVIAKRPAPIALPN 461
            NL  L+  PL+  G   +     A P+
Sbjct: 110 GNLTPLEQTPLASVGKNLEEKEAAAKPS 137


>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
           zf-fungal binuclear cluster type |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 977

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 9/27 (33%), Positives = 15/27 (55%)
 Frame = +3

Query: 36  LEVRLLWGSVLATSSRCPALWS*WCHR 116
           LE++  WGS+ A   +   +W+ W  R
Sbjct: 850 LEMKNYWGSISALCDKMSEIWADWVQR 876


>SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein
           kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 836

 Score = 25.8 bits (54), Expect = 5.7
 Identities = 10/26 (38%), Positives = 14/26 (53%)
 Frame = -3

Query: 663 SPRSLIVDSCSKLEQHSTLSRSILLI 586
           SP +L   +CS L  HST    + L+
Sbjct: 28  SPNNLTEQTCSPLRAHSTFKEPVFLL 53


>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 2052

 Score = 25.8 bits (54), Expect = 5.7
 Identities = 12/24 (50%), Positives = 14/24 (58%)
 Frame = +1

Query: 532 VKSAHFLTNRPKSAKSLINQKNRP 603
           VK   FLTN   +  SL+ Q NRP
Sbjct: 675 VKDYDFLTNLNATTLSLLTQSNRP 698


>SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog
           Chs2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 926

 Score = 25.8 bits (54), Expect = 5.7
 Identities = 11/27 (40%), Positives = 13/27 (48%)
 Frame = -1

Query: 479 PFXCATVGKGDRCGPLRYYASWRKGDV 399
           P  C  V  G R GP   Y +W+  DV
Sbjct: 392 PKVCLFVRNGARLGPTSIYHAWKAFDV 418


>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 630

 Score = 25.4 bits (53), Expect = 7.6
 Identities = 9/24 (37%), Positives = 14/24 (58%)
 Frame = -3

Query: 144 EAFSLFDKDGDGTITTKELGTVMR 73
           + F  FD+D DG +  +EL  + R
Sbjct: 311 DLFYQFDRDNDGALNNEELSALFR 334


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,752,052
Number of Sequences: 5004
Number of extensions: 54494
Number of successful extensions: 119
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 119
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)