BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30128 (628 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g55780.1 68418.m06952 DC1 domain-containing protein contains ... 31 0.47 At3g61820.1 68416.m06939 aspartyl protease family protein contai... 31 0.83 At3g14610.1 68416.m01850 cytochrome P450, putative similar to GB... 29 2.5 At4g18790.1 68417.m02775 NRAMP metal ion transporter 5, putative... 29 3.3 At3g56780.1 68416.m06315 F-box family protein contains F-box dom... 28 5.8 At4g25160.1 68417.m03622 protein kinase family protein contains ... 27 7.7 >At5g55780.1 68418.m06952 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 685 Score = 31.5 bits (68), Expect = 0.47 Identities = 13/25 (52%), Positives = 15/25 (60%) Frame = +3 Query: 369 CRYPFGLAINGDKLYWSDWRTLKIE 443 CR PF + GDKL + W LKIE Sbjct: 651 CRCPFPIFYKGDKLIFCSWYCLKIE 675 >At3g61820.1 68416.m06939 aspartyl protease family protein contains Pfam domain, PF00026: eukaryotic aspartyl protease Length = 483 Score = 30.7 bits (66), Expect = 0.83 Identities = 16/59 (27%), Positives = 31/59 (52%) Frame = -2 Query: 279 STSRWPQSWKLHIWATEED*SFRAVHVRPLDLRIASIPVSPEDFPSLRMYGDAPRIRNV 103 +T WP+S L + E + +VH+ +D + SP D +LR+ D+ R++++ Sbjct: 39 ATLSWPESESLTDESLSESTTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSI 97 >At3g14610.1 68416.m01850 cytochrome P450, putative similar to GB:Q05047 from [Catharanthus roseus] Length = 512 Score = 29.1 bits (62), Expect = 2.5 Identities = 11/37 (29%), Positives = 22/37 (59%) Frame = -3 Query: 125 MPLGLEMFCTENKTFLTFVSKLATSIVSFLESVQNMF 15 +PL L+M + KTF ++ L T +++ E ++ +F Sbjct: 81 LPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVF 117 >At4g18790.1 68417.m02775 NRAMP metal ion transporter 5, putative (NRAMP5) identical to heavy metal transporter [Arabidopsis thaliana]gi|12657269|emb|CAC27822; similar to metal transporter Nramp3 [Arabidopsis thaliana] gi|6468012|gb|AAF13278; member of the natural resistance-associated macrophage protein (NRAMP) metal transporter family, PMID:11500563 Length = 530 Score = 28.7 bits (61), Expect = 3.3 Identities = 16/77 (20%), Positives = 36/77 (46%) Frame = -1 Query: 499 IVDLGMSICPLAWVIVSMISIFNVLQSLQYNLSPLMARPKGYLQFSAIVSICRLCRSMHL 320 + +G S+ LAW + + + N L + ++ + G+L F +V ++L Sbjct: 448 VFKIGPSLEKLAWTVAVFVMMINGYLLLDFFMAEVEGFLVGFLVFGGVVGYISFI--IYL 505 Query: 319 MTWRPASAKQTRSLDQS 269 +++R + + SL+ S Sbjct: 506 VSYRSSQSSSWSSLEMS 522 >At3g56780.1 68416.m06315 F-box family protein contains F-box domain Pfam:PF00646 Length = 431 Score = 27.9 bits (59), Expect = 5.8 Identities = 25/87 (28%), Positives = 42/87 (48%), Gaps = 2/87 (2%) Frame = -1 Query: 562 LRANVGSPGTLARRVGNTYNRIVDLGMSICPLAWV--IVSMISIFNVLQSLQYNLSPLMA 389 +R +G P L +T++RIV L +SIC V +V ++ F L L+ L+ Sbjct: 330 VRVRLGVPSKLED--ASTFSRIVHLELSICSERSVEMLVDLLLCFTKLVVLKLEHVYLLT 387 Query: 388 RPKGYLQFSAIVSICRLCRSMHLMTWR 308 P G + ++V C L S+ + W+ Sbjct: 388 -PLGRWEPPSLVPEC-LLSSLEALEWK 412 >At4g25160.1 68417.m03622 protein kinase family protein contains Pfam profile: PF00069 Eukaryotic protein kinase domain Length = 835 Score = 27.5 bits (58), Expect = 7.7 Identities = 12/38 (31%), Positives = 23/38 (60%) Frame = -2 Query: 279 STSRWPQSWKLHIWATEED*SFRAVHVRPLDLRIASIP 166 S S++ +W + +ATE + F+ +H+ P+ I S+P Sbjct: 30 SKSKYVVTWAIEKFATEGNVGFKLLHIHPM---ITSVP 64 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,426,602 Number of Sequences: 28952 Number of extensions: 310677 Number of successful extensions: 978 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 961 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 978 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1275599520 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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