BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30125 (477 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces... 142 2e-35 SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 28 0.84 SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 26 3.4 SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb... 25 5.9 SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 25 7.9 SPBC336.11 |||GARP complex subunit Vps52 |Schizosaccharomyces po... 25 7.9 >SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces pombe|chr 2|||Manual Length = 249 Score = 142 bits (345), Expect = 2e-35 Identities = 64/87 (73%), Positives = 76/87 (87%) Frame = +3 Query: 3 QAESLRYKLIGGLAVRRACYGVLRFIMESGARGCEVVVSGKLRGQRAKSMKFVDGLMIHS 182 Q ESLRYKL+ GLAVRRA YGVLR++ME+GA+GCEVV+SGKLR RAKSMKF DG MIHS Sbjct: 103 QCESLRYKLLAGLAVRRAAYGVLRYVMEAGAKGCEVVISGKLRAARAKSMKFADGFMIHS 162 Query: 183 GDPCNDYVNTATRHVLLRQGVLGIRSK 263 G P D++++ATRHVLLRQGVLG++ K Sbjct: 163 GQPAVDFIDSATRHVLLRQGVLGVKVK 189 >SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2111 Score = 27.9 bits (59), Expect = 0.84 Identities = 15/52 (28%), Positives = 26/52 (50%) Frame = +3 Query: 69 LRFIMESGARGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPCNDYVNTATRH 224 + F ME + + ++ +L+ S K D ++I GD ND + T+ RH Sbjct: 1868 IHFAMEFLHKNTKSLLDSELKDGNYISAKGKDVIVIGGGDTGNDCLGTSVRH 1919 >SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 2685 Score = 25.8 bits (54), Expect = 3.4 Identities = 9/31 (29%), Positives = 16/31 (51%), Gaps = 1/31 (3%) Frame = +2 Query: 134 STCQINEVCR-WTHDPLWRPLQ*LRQHCYQT 223 + C + CR W P W+ + + Q+C+ T Sbjct: 1420 TVCNRKKACRLWNFKPHWQVITRIPQYCHDT 1450 >SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 25.0 bits (52), Expect = 5.9 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = +3 Query: 132 GQRAKSMKFVDGLMIHSGDPCNDYVN 209 G+R K + F D H G P DY++ Sbjct: 218 GRREKLLVFGDDYDSHDGTPIRDYIH 243 >SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|Schizosaccharomyces pombe|chr 2|||Manual Length = 1173 Score = 24.6 bits (51), Expect = 7.9 Identities = 9/14 (64%), Positives = 10/14 (71%) Frame = +3 Query: 189 PCNDYVNTATRHVL 230 PC+DYV A R VL Sbjct: 679 PCSDYVEAAVRQVL 692 >SPBC336.11 |||GARP complex subunit Vps52 |Schizosaccharomyces pombe|chr 2|||Manual Length = 508 Score = 24.6 bits (51), Expect = 7.9 Identities = 8/11 (72%), Positives = 10/11 (90%) Frame = +1 Query: 295 RPEEATTRPHP 327 +PEE T+RPHP Sbjct: 404 KPEEITSRPHP 414 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,790,537 Number of Sequences: 5004 Number of extensions: 34787 Number of successful extensions: 83 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 82 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 83 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 184476110 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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