BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30107 (776 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 130 2e-31 SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 117 2e-27 SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 51 2e-07 SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 31 0.18 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.24 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.3 SPAC607.06c |||metallopeptidase|Schizosaccharomyces pombe|chr 1|... 27 2.3 SPBC16G5.18 |erg24||C-14 sterol reductase Erg24|Schizosaccharomy... 27 3.0 SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating tra... 27 4.0 SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma... 27 4.0 SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyce... 26 5.2 SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces ... 26 5.2 SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||M... 26 5.2 SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 25 9.2 SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 25 9.2 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 9.2 >SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 130 bits (314), Expect = 2e-31 Identities = 67/88 (76%), Positives = 77/88 (87%) Frame = +1 Query: 256 ELIMKSIIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAG 435 +LI+K+ IPVVMAGIIAIYGLVV+VLI+G L++ + LY GFI LGAGL+VG +GLAAG Sbjct: 49 DLIVKNTIPVVMAGIIAIYGLVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLAAG 106 Query: 436 FAIGIVGDAGVRGTAQQPRLFVGMILFL 519 FAIGIVGDAGVRGTAQQPRLFV MIL L Sbjct: 107 FAIGIVGDAGVRGTAQQPRLFVAMILIL 134 Score = 60.5 bits (140), Expect = 3e-10 Identities = 24/36 (66%), Positives = 31/36 (86%) Frame = +2 Query: 131 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAA 238 P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+A Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISA 42 Score = 37.5 bits (83), Expect = 0.002 Identities = 18/20 (90%), Positives = 19/20 (95%) Frame = +3 Query: 510 LILIFAEVLGLYGLIVAIYL 569 LILIFAEVLGLYGLIVA+ L Sbjct: 132 LILIFAEVLGLYGLIVALLL 151 >SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 162 Score = 117 bits (282), Expect = 2e-27 Identities = 53/88 (60%), Positives = 71/88 (80%) Frame = +1 Query: 256 ELIMKSIIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAG 435 E++MKS+IPVVM+GII +YGLV++VLIAG + +Y L+ GFIHL AGLAVG +G+AAG Sbjct: 49 EIVMKSLIPVVMSGIIGVYGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVAAG 108 Query: 436 FAIGIVGDAGVRGTAQQPRLFVGMILFL 519 +AIG+VGD GV+ +Q R+FV M+L L Sbjct: 109 YAIGVVGDRGVQSFMRQDRIFVSMVLIL 136 Score = 49.6 bits (113), Expect = 5e-07 Identities = 21/36 (58%), Positives = 26/36 (72%) Frame = +2 Query: 131 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAA 238 PIY FFG G ++++FS LGA YGTA +G GIAA Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAA 42 Score = 35.9 bits (79), Expect = 0.006 Identities = 17/20 (85%), Positives = 18/20 (90%) Frame = +3 Query: 510 LILIFAEVLGLYGLIVAIYL 569 LILIFAEVLGLYGLIV + L Sbjct: 134 LILIFAEVLGLYGLIVGLIL 153 >SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces pombe|chr 1|||Manual Length = 199 Score = 50.8 bits (116), Expect = 2e-07 Identities = 25/88 (28%), Positives = 45/88 (51%), Gaps = 6/88 (6%) Frame = +1 Query: 268 KSIIPVVMAGIIAIYGLVVAVLIAGALQE--PANY----PLYKGFIHLGAGLAVGFSGLA 429 K++I ++ ++AIY L++A++ + + + PA + Y GF G+ VG L Sbjct: 85 KNLISIIFCEVVAIYSLIIAIVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLI 144 Query: 430 AGFAIGIVGDAGVRGTAQQPRLFVGMIL 513 G +GI G + AQ LFV +++ Sbjct: 145 CGVCVGITGSSAALADAQDASLFVKVLV 172 Score = 29.1 bits (62), Expect = 0.74 Identities = 12/28 (42%), Positives = 18/28 (64%) Frame = +2 Query: 149 FGVMGAASAIIFSALGAAYGTAKSGTGI 232 +G++G AS + F +GAA+G GT I Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSI 72 Score = 25.4 bits (53), Expect = 9.2 Identities = 10/20 (50%), Positives = 15/20 (75%) Frame = +3 Query: 510 LILIFAEVLGLYGLIVAIYL 569 ++ IF VLGL+GLIV + + Sbjct: 172 VVEIFGSVLGLFGLIVGLLI 191 >SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 288 Score = 31.1 bits (67), Expect = 0.18 Identities = 21/68 (30%), Positives = 30/68 (44%) Frame = +1 Query: 274 IIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIV 453 II +AG+IA + +++ IAG + G ++ G L LA GF I Sbjct: 193 IITATIAGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTS 246 Query: 454 GDAGVRGT 477 GD V T Sbjct: 247 GDVCVSNT 254 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 30.7 bits (66), Expect = 0.24 Identities = 14/34 (41%), Positives = 18/34 (52%) Frame = -1 Query: 479 AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNP 378 +VP P++P MP P+A P A AP NP Sbjct: 1713 SVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 28.3 bits (60), Expect = 1.3 Identities = 26/65 (40%), Positives = 30/65 (46%), Gaps = 2/65 (3%) Frame = -1 Query: 479 AVPRTPAS--PTMPMAKPAARPENPTAKPAPKWMNPL*RG*LAGSWRAPAIRTATTRP*M 306 A P P S P++PM PAA P P+A AP PL AG AP + A P Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP----PL----PAGMPAAPPLPPAAPAPPP 478 Query: 305 AIIPA 291 A PA Sbjct: 479 APAPA 483 >SPAC607.06c |||metallopeptidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 612 Score = 27.5 bits (58), Expect = 2.3 Identities = 16/38 (42%), Positives = 21/38 (55%) Frame = +3 Query: 660 GMVVAGSGSNLHRVSEHPSHSCILHLFPCSFFTYPRPV 773 G V G G+++H++ SHS LH FP S Y PV Sbjct: 235 GHVALGGGTDMHKLGVFGSHS--LHSFP-SALEYVVPV 269 >SPBC16G5.18 |erg24||C-14 sterol reductase Erg24|Schizosaccharomyces pombe|chr 2|||Manual Length = 424 Score = 27.1 bits (57), Expect = 3.0 Identities = 8/20 (40%), Positives = 13/20 (65%) Frame = +2 Query: 698 GFGAPFPLLYITFVSLLVFH 757 GFG+P P Y+ + +L+ H Sbjct: 372 GFGSPIPYFYVAYFGVLLVH 391 >SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating transcription Rct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 432 Score = 26.6 bits (56), Expect = 4.0 Identities = 10/23 (43%), Positives = 14/23 (60%) Frame = -1 Query: 89 TGPKNCDDYLHTLTPRNYFCTDK 21 TG KNCDD+L +P + D+ Sbjct: 402 TGNKNCDDHLRDKSPERRYRYDR 424 >SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 629 Score = 26.6 bits (56), Expect = 4.0 Identities = 12/26 (46%), Positives = 17/26 (65%) Frame = +1 Query: 496 FVGMILFLFSLKYWVFTDLSSPSTCT 573 F G+ +F+FS+ YW F +SS S T Sbjct: 564 FCGIYVFIFSVFYWFF-KISSSSLAT 588 >SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyces pombe|chr 2|||Manual Length = 760 Score = 26.2 bits (55), Expect = 5.2 Identities = 14/38 (36%), Positives = 22/38 (57%) Frame = -3 Query: 387 DEPFVKGVVGWLLEGTSNQDSHDQTVDGNNTRHDDRND 274 DEPF+K +L+ SN++S+ VD N+ D +D Sbjct: 48 DEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSD 85 >SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1888 Score = 26.2 bits (55), Expect = 5.2 Identities = 13/47 (27%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Frame = -3 Query: 744 RETNVIYKSGKGAPKPYASWT-RYRRRPSLCASSDSDNEHRGSEHGR 607 + NVI ++ + KP + T R+RR ++ + SD D + +H + Sbjct: 164 KPVNVIVRNRASSRKPPLTSTHRFRRYGAVISDSDDDESNTEQDHSK 210 >SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 629 Score = 26.2 bits (55), Expect = 5.2 Identities = 13/44 (29%), Positives = 23/44 (52%) Frame = +1 Query: 433 GFAIGIVGDAGVRGTAQQPRLFVGMILFLFSLKYWVFTDLSSPS 564 G A+G G+ G R +++P + + + Y+ F DLS P+ Sbjct: 520 GSAVGQNGNVGPRQISEEPMSIIFNLGISNNWAYYYFRDLSFPA 563 >SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 25.4 bits (53), Expect = 9.2 Identities = 8/13 (61%), Positives = 10/13 (76%) Frame = -1 Query: 89 TGPKNCDDYLHTL 51 TGP N DDY+H + Sbjct: 369 TGPSNTDDYIHRI 381 >SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 1563 Score = 25.4 bits (53), Expect = 9.2 Identities = 10/20 (50%), Positives = 15/20 (75%) Frame = +2 Query: 704 GAPFPLLYITFVSLLVFHIS 763 GA P+ Y+TFVSL V+ ++ Sbjct: 1542 GANKPIAYLTFVSLFVYIVT 1561 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 25.4 bits (53), Expect = 9.2 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 4/34 (11%) Frame = -1 Query: 479 AVPRTPASPTMPMA----KPAARPENPTAKPAPK 390 +VP+ PA+P +P A +P A P P AP+ Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQ 546 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,570,466 Number of Sequences: 5004 Number of extensions: 77907 Number of successful extensions: 262 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 240 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 261 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 375345278 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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