Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30095
(565 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein L7|... 114 7e-27
SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomy... 111 5e-26
SPAC664.06 |rpl703|rpl7|60S ribosomal protein L7|Schizosaccharom... 82 6e-17
SPCC1672.10 |mis16||kinetochore protein Mis16 |Schizosaccharomyc... 29 0.47
SPAC1952.01 ||SPAC1B3.19|Pig-U|Schizosaccharomyces pombe|chr 1||... 28 0.82
SPCC830.10 |||nucleoside triphosphatase |Schizosaccharomyces pom... 28 1.1
SPCC63.07 |||tRNA guanylyltransferase |Schizosaccharomyces pombe... 26 3.3
SPCPB16A4.02c |||conserved fungal protein|Schizosaccharomyces po... 26 4.4
SPAPB8E5.03 |mae1||malic acid transport protein Mae1 |Schizosacc... 25 7.7
SPBC30B4.07c |tfb4||transcription factor TFIIH complex subunit T... 25 7.7
>SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein
L7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 250
Score = 114 bits (275), Expect = 7e-27
Identities = 52/102 (50%), Positives = 73/102 (71%), Gaps = 1/102 (0%)
Frame = +2
Query: 206 GIPQLKECP*VSIQTWIAKLSGQRIPITSNSIVEKRLHKHNIICVEDLIHEIFTVGEKFK 385
GIP K + + K++ QRIP++ N+I+E L K++I+ VEDLIHEI+TVG FK
Sbjct: 144 GIPNHKTVRELIYKRGFGKVNKQRIPLSDNAIIEAALGKYSILSVEDLIHEIYTVGPNFK 203
Query: 386 YASNFLWPFKLNNPTGGWR-KKTIHYVDGGDFGNREDKINDL 508
A+NFLWPFKL++P GGWR +K H+++GGD G R++ IN L
Sbjct: 204 QAANFLWPFKLSSPLGGWRERKFKHFIEGGDAGKRDEHINGL 245
Score = 79.8 bits (188), Expect = 3e-16
Identities = 33/55 (60%), Positives = 44/55 (80%)
Frame = +3
Query: 90 EVRKVLQLFRLRQINNGVFVRLNKATVNMLRIAEPYIAWGYPNLKSVRELVYKRG 254
+ RK++QL RL QINNG+FV+ NKA ML++ EPY+ +G PN K+VREL+YKRG
Sbjct: 105 KARKIMQLLRLLQINNGIFVKFNKAIKEMLQVVEPYVTYGIPNHKTVRELIYKRG 159
Score = 49.6 bits (113), Expect = 3e-07
Identities = 22/39 (56%), Positives = 27/39 (69%)
Frame = +1
Query: 1 QARNRGNYYVPGEAKLAFVIRIRGINQVSPKSVKFCNCL 117
+AR GNY+VP E KL FV+RIRGIN + PK+ K L
Sbjct: 75 KARAEGNYFVPHEPKLIFVVRIRGINNIPPKARKIMQLL 113
>SPBC18H10.12c |rpl701||60S ribosomal protein L7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 251
Score = 111 bits (268), Expect = 5e-26
Identities = 49/102 (48%), Positives = 73/102 (71%), Gaps = 1/102 (0%)
Frame = +2
Query: 206 GIPQLKECP*VSIQTWIAKLSGQRIPITSNSIVEKRLHKHNIICVEDLIHEIFTVGEKFK 385
GIP LK + + K++ QRI ++ N+I+E L K++I+ +EDLIHEI+TVG FK
Sbjct: 145 GIPNLKTVRELLYKRGFGKVNKQRIALSDNAIIEAALGKYSILSIEDLIHEIYTVGPNFK 204
Query: 386 YASNFLWPFKLNNPTGGWR-KKTIHYVDGGDFGNREDKINDL 508
A+NF+WPF+L++P GGWR +K H+++GGD G R++ IN L
Sbjct: 205 QAANFIWPFQLSSPLGGWRDRKFKHFIEGGDAGKRDEHINSL 246
Score = 84.2 bits (199), Expect = 1e-17
Identities = 36/55 (65%), Positives = 46/55 (83%)
Frame = +3
Query: 90 EVRKVLQLFRLRQINNGVFVRLNKATVNMLRIAEPYIAWGYPNLKSVRELVYKRG 254
+ RK++QL RL QINNGVFV+ NKAT ML++ EPY+ +G PNLK+VREL+YKRG
Sbjct: 106 KARKIMQLLRLIQINNGVFVKFNKATKEMLQVVEPYVTYGIPNLKTVRELLYKRG 160
Score = 52.8 bits (121), Expect = 3e-08
Identities = 24/39 (61%), Positives = 27/39 (69%)
Frame = +1
Query: 1 QARNRGNYYVPGEAKLAFVIRIRGINQVSPKSVKFCNCL 117
+AR GNYYVP E KL FVIRIRGIN + PK+ K L
Sbjct: 76 KARAEGNYYVPDETKLVFVIRIRGINNIPPKARKIMQLL 114
>SPAC664.06 |rpl703|rpl7|60S ribosomal protein
L7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 249
Score = 81.8 bits (193), Expect = 6e-17
Identities = 37/55 (67%), Positives = 44/55 (80%)
Frame = +3
Query: 90 EVRKVLQLFRLRQINNGVFVRLNKATVNMLRIAEPYIAWGYPNLKSVRELVYKRG 254
++RKVL+L RL +INN VFVR NKA MLRI EPY+ +G PNL SVREL+YKRG
Sbjct: 104 KIRKVLRLLRLSRINNAVFVRNNKAVAQMLRIVEPYVMYGIPNLHSVRELIYKRG 158
Score = 78.6 bits (185), Expect = 6e-16
Identities = 38/102 (37%), Positives = 60/102 (58%), Gaps = 1/102 (0%)
Frame = +2
Query: 206 GIPQLKECP*VSIQTWIAKLSGQRIPITSNSIVEKRLHKHNIICVEDLIHEIFTVGEKFK 385
GIP L + + K++GQRI ++ N+++E+ L K+++I +ED+IHEI+ VG FK
Sbjct: 143 GIPNLHSVRELIYKRGFGKINGQRIALSDNALIEEALGKYDVISIEDIIHEIYNVGSHFK 202
Query: 386 YASNFLWPFKLNNPTGG-WRKKTIHYVDGGDFGNREDKINDL 508
+ FLWPF L KK H+ +G G ++IN+L
Sbjct: 203 EVTKFLWPFTLTPVKHSLMEKKVKHFNEGRKAGYCGEEINEL 244
Score = 40.3 bits (90), Expect = 2e-04
Identities = 18/38 (47%), Positives = 24/38 (63%)
Frame = +1
Query: 4 ARNRGNYYVPGEAKLAFVIRIRGINQVSPKSVKFCNCL 117
A+N+G+ +VP E KL FVIRI G+ + PK K L
Sbjct: 75 AKNKGDIFVPDETKLLFVIRIAGVKNMPPKIRKVLRLL 112
>SPCC1672.10 |mis16||kinetochore protein Mis16 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 430
Score = 29.1 bits (62), Expect = 0.47
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = -2
Query: 444 LRQPPVGLFNLKGHRKLLAYLNFSPTVKISWMRSSTQIMLCLWSL 310
LR P L L+GH + L +SP + SST +C+W L
Sbjct: 311 LRNPYQRLHTLEGHEDEVYGLEWSPHDEPILASSSTDRRVCIWDL 355
>SPAC1952.01 ||SPAC1B3.19|Pig-U|Schizosaccharomyces pombe|chr
1|||Manual
Length = 408
Score = 28.3 bits (60), Expect = 0.82
Identities = 17/59 (28%), Positives = 27/59 (45%)
Frame = -1
Query: 388 VLELFSNSEDLMDEVLNTDNVVFMEPLLNNAVGSDWYTLSTQLGDPRLYTNSRTLFKLG 212
+L +F+ +D M L T+N + +L +A W TL G+ Y S + LG
Sbjct: 319 LLPIFNKVQDRMRYSLLTNNAIVFALVLGSAFYHSWITLG--CGNANFYYASNLILALG 375
>SPCC830.10 |||nucleoside triphosphatase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 188
Score = 27.9 bits (59), Expect = 1.1
Identities = 11/32 (34%), Positives = 15/32 (46%)
Frame = -1
Query: 457 MNGLLAPTTSWIVQFEGPQEITRVLELFSNSE 362
MNGL P W + GP + R++ F E
Sbjct: 75 MNGLPGPYVKWFLNSVGPDGLYRMVSAFDTKE 106
>SPCC63.07 |||tRNA guanylyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 261
Score = 26.2 bits (55), Expect = 3.3
Identities = 11/40 (27%), Positives = 22/40 (55%)
Frame = +3
Query: 210 YPNLKSVRELVYKRGSPS*VDNVYQSLPTALLRRGSINTT 329
YPN+K +R+ ++ R ++N+Y + L+ +G T
Sbjct: 133 YPNMKVLRDYLHWRQVDCHINNLYNTTFWMLILKGGFTNT 172
>SPCPB16A4.02c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 328
Score = 25.8 bits (54), Expect = 4.4
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +1
Query: 433 WLAQEDHSLCRRGRLW*PRRQDQRSF 510
WL ++ H+ + +LW R+DQ SF
Sbjct: 31 WLIKKGHATSTKKQLWAVLRRDQLSF 56
>SPAPB8E5.03 |mae1||malic acid transport protein Mae1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 438
Score = 25.0 bits (52), Expect = 7.7
Identities = 11/49 (22%), Positives = 24/49 (48%)
Frame = -2
Query: 423 LFNLKGHRKLLAYLNFSPTVKISWMRSSTQIMLCLWSLFSTMLLEVIGI 277
LF+L G L ++ + T+K SW ++ + L + ++++ I
Sbjct: 76 LFSLFGSCMLFRFIKYPSTIKDSWNHHLEKLFIATCLLSISTFIDMLAI 124
>SPBC30B4.07c |tfb4||transcription factor TFIIH complex subunit Tfb4
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 25.0 bits (52), Expect = 7.7
Identities = 14/59 (23%), Positives = 31/59 (52%)
Frame = -1
Query: 496 LVFAVTKVSPVDIMNGLLAPTTSWIVQFEGPQEITRVLELFSNSEDLMDEVLNTDNVVF 320
L+ + KVS +++G L+ ++I Q + + + +FS + D+ + + T N +F
Sbjct: 121 LMSSTDKVSRKTMISGALSRALAYINQVQNKNTLRSRILIFSLTGDVALQYIPTMNCIF 179
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,546,958
Number of Sequences: 5004
Number of extensions: 55715
Number of successful extensions: 152
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 238029836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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