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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30089
         (683 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ...   102   7e-21
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L...   102   7e-21
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet...   102   7e-21
UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ...   102   9e-21
UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo...    79   8e-14
UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:...    69   1e-10
UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1; ...    54   2e-06
UniRef50_P06219 Cluster: Beta-galactosidase; n=11; Gammaproteoba...    54   3e-06
UniRef50_A7BPF2 Cluster: LacZ alpha peptide; n=1; Beggiatoa sp. ...    46   9e-04
UniRef50_P81650 Cluster: Beta-galactosidase; n=26; Gammaproteoba...    44   0.003
UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia sp...    41   0.024
UniRef50_A6FJQ2 Cluster: 50S ribosomal protein L5; n=8; Bacteria...    40   0.056
UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3; ...    37   0.40 
UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus la...    36   0.92 
UniRef50_A0UVE2 Cluster: Glycoside hydrolase family 2, TIM barre...    36   1.2  
UniRef50_Q9JN59 Cluster: Beta-galactosidase; n=16; Vibrio choler...    34   2.8  
UniRef50_Q15XN9 Cluster: Glycoside hydrolase family 2, TIM barre...    34   2.8  
UniRef50_Q5DC94 Cluster: SJCHGC09076 protein; n=1; Schistosoma j...    34   2.8  
UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1; ...    34   3.7  
UniRef50_A6G4K3 Cluster: Putative uncharacterized protein; n=1; ...    33   8.6  

>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
           organisms|Rep: LacZ-alpha peptide - Escherichia coli
          Length = 90

 Score =  102 bits (245), Expect = 7e-21
 Identities = 46/47 (97%), Positives = 47/47 (100%)
 Frame = +1

Query: 82  LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRT 222
           LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR+
Sbjct: 22  LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS 68


>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
           protein - Phage M13mp18
          Length = 102

 Score =  102 bits (245), Expect = 7e-21
 Identities = 46/47 (97%), Positives = 47/47 (100%)
 Frame = +1

Query: 82  LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRT 222
           LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR+
Sbjct: 26  LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS 72


>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
           Beta-galactosidase - Escherichia coli (strain K12)
          Length = 1024

 Score =  102 bits (245), Expect = 7e-21
 Identities = 46/47 (97%), Positives = 47/47 (100%)
 Frame = +1

Query: 82  LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRT 222
           LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR+
Sbjct: 8   LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS 54


>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
           Erwinia amylovora|Rep: Putative uncharacterized protein
           - Erwinia amylovora (Fire blight bacteria)
          Length = 123

 Score =  102 bits (244), Expect = 9e-21
 Identities = 46/46 (100%), Positives = 46/46 (100%)
 Frame = +1

Query: 82  LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR 219
           LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
Sbjct: 68  LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR 113


>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
           Eukaryota|Rep: beta-galactosidase - Entamoeba
           histolytica HM-1:IMSS
          Length = 86

 Score = 79.4 bits (187), Expect = 8e-14
 Identities = 43/69 (62%), Positives = 51/69 (73%), Gaps = 2/69 (2%)
 Frame = +2

Query: 80  HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNS--CAPEWRMANCKR* 253
           HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVI++  A    P+    + +WRMA   R 
Sbjct: 5   HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVISEE-ARTDRPSQQLRSLKWRMAPEWRY 63

Query: 254 FLLKFALNF 280
           FLL  ++ +
Sbjct: 64  FLLTQSVRY 72


>UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:
           Beta-galactosidase - Yersinia pseudotuberculosis
          Length = 1066

 Score = 68.5 bits (160), Expect = 1e-10
 Identities = 29/47 (61%), Positives = 35/47 (74%)
 Frame = +1

Query: 82  LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRT 222
           L  +L RRDWENP +TQ +RL AHPPF SWR+ E A+ DRPS Q +T
Sbjct: 15  LPQILSRRDWENPQITQYHRLEAHPPFHSWRDVESAQKDRPSPQQQT 61


>UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1;
           Enterobacter sakazakii ATCC BAA-894|Rep: Putative
           uncharacterized protein - Enterobacter sakazakii ATCC
           BAA-894
          Length = 1043

 Score = 54.4 bits (125), Expect = 2e-06
 Identities = 20/42 (47%), Positives = 28/42 (66%)
 Frame = +1

Query: 82  LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS 207
           LA +L R DW+NP +T +NRL +H P   WR+++ AR   PS
Sbjct: 18  LATILARNDWQNPAITSVNRLPSHTPLHGWRDADRARRGEPS 59


>UniRef50_P06219 Cluster: Beta-galactosidase; n=11;
           Gammaproteobacteria|Rep: Beta-galactosidase - Klebsiella
           pneumoniae
          Length = 1034

 Score = 54.0 bits (124), Expect = 3e-06
 Identities = 24/43 (55%), Positives = 28/43 (65%)
 Frame = +1

Query: 91  VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR 219
           VL R DW N  +T LNRL AHP FASWR+   AR + PS + R
Sbjct: 17  VLAREDWHNQTITHLNRLPAHPVFASWRDELAARDNLPSSRRR 59


>UniRef50_A7BPF2 Cluster: LacZ alpha peptide; n=1; Beggiatoa sp.
           SS|Rep: LacZ alpha peptide - Beggiatoa sp. SS
          Length = 73

 Score = 46.0 bits (104), Expect = 9e-04
 Identities = 21/28 (75%), Positives = 21/28 (75%)
 Frame = -1

Query: 563 GFPRQALNRGLPLGFRFSVYGTSTPKNL 480
           GFPRQALNRGLPLGFRFS      PK L
Sbjct: 45  GFPRQALNRGLPLGFRFSALRHLDPKKL 72


>UniRef50_P81650 Cluster: Beta-galactosidase; n=26;
           Gammaproteobacteria|Rep: Beta-galactosidase -
           Pseudoalteromonas haloplanktis (Alteromonas
           haloplanktis)
          Length = 1039

 Score = 44.4 bits (100), Expect = 0.003
 Identities = 17/41 (41%), Positives = 27/41 (65%)
 Frame = +1

Query: 91  VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQ 213
           ++ RRDWENP   Q+N++ AH P   ++  E+AR +  SQ+
Sbjct: 7   IINRRDWENPITVQVNQVKAHSPLNGFKTIEDARENTQSQK 47


>UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia
           spumigena CCY 9414|Rep: Beta-D-galactosidase - Nodularia
           spumigena CCY 9414
          Length = 72

 Score = 41.1 bits (92), Expect = 0.024
 Identities = 17/18 (94%), Positives = 18/18 (100%)
 Frame = +1

Query: 169 WRNSEEARTDRPSQQLRT 222
           WRNSEEARTDRPSQQLR+
Sbjct: 47  WRNSEEARTDRPSQQLRS 64


>UniRef50_A6FJQ2 Cluster: 50S ribosomal protein L5; n=8;
           Bacteria|Rep: 50S ribosomal protein L5 - Moritella sp.
           PE36
          Length = 45

 Score = 39.9 bits (89), Expect = 0.056
 Identities = 22/29 (75%), Positives = 23/29 (79%)
 Frame = -3

Query: 240 FAIRHSGAQLLGRAIGAGLFAITPAGERG 154
           FAI+   AQLLGRAIGAGLFAITP  E G
Sbjct: 12  FAIQ--AAQLLGRAIGAGLFAITPEFELG 38


>UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3;
           Plasmodium (Vinckeia)|Rep: Putative uncharacterized
           protein - Plasmodium berghei
          Length = 275

 Score = 37.1 bits (82), Expect = 0.40
 Identities = 16/16 (100%), Positives = 16/16 (100%)
 Frame = +2

Query: 29  RGGARYPIRPIVSRIT 76
           RGGARYPIRPIVSRIT
Sbjct: 260 RGGARYPIRPIVSRIT 275


>UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus
           lactis|Rep: Beta-galactosidase - Lactococcus lactis
           subsp. lactis (Streptococcus lactis)
          Length = 998

 Score = 35.9 bits (79), Expect = 0.92
 Identities = 14/23 (60%), Positives = 17/23 (73%)
 Frame = +1

Query: 91  VLQRRDWENPGVTQLNRLAAHPP 159
           VL+R+DWENP V+  NRL  H P
Sbjct: 9   VLERKDWENPVVSNWNRLPMHTP 31


>UniRef50_A0UVE2 Cluster: Glycoside hydrolase family 2, TIM barrel;
           n=1; Clostridium cellulolyticum H10|Rep: Glycoside
           hydrolase family 2, TIM barrel - Clostridium
           cellulolyticum H10
          Length = 1033

 Score = 35.5 bits (78), Expect = 1.2
 Identities = 12/29 (41%), Positives = 20/29 (68%)
 Frame = +1

Query: 103 RDWENPGVTQLNRLAAHPPFASWRNSEEA 189
           R+WEN  +TQ+NR   H P+ ++ + E+A
Sbjct: 3   REWENQYITQINRYPMHSPYGAYESVEQA 31


>UniRef50_Q9JN59 Cluster: Beta-galactosidase; n=16; Vibrio
           cholerae|Rep: Beta-galactosidase - Vibrio cholerae
          Length = 56

 Score = 34.3 bits (75), Expect = 2.8
 Identities = 13/36 (36%), Positives = 21/36 (58%)
 Frame = +1

Query: 91  VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTD 198
           +L  +DW+NP + + +    H P  S+R  +EAR D
Sbjct: 7   ILLSQDWQNPHIVKWHCRTPHVPLHSYRTEQEARLD 42


>UniRef50_Q15XN9 Cluster: Glycoside hydrolase family 2, TIM barrel
           precursor; n=1; Pseudoalteromonas atlantica T6c|Rep:
           Glycoside hydrolase family 2, TIM barrel precursor -
           Pseudoalteromonas atlantica (strain T6c / BAA-1087)
          Length = 1079

 Score = 34.3 bits (75), Expect = 2.8
 Identities = 17/42 (40%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
 Frame = +1

Query: 100 RRDWENPGVTQLNRLAAHPPFASWRNSEEART-DRPSQQLRT 222
           + DWENP V Q+NRL A     S+   E+A T DR    +++
Sbjct: 31  KNDWENPDVIQINRLPARATSYSFDTPEQALTRDRNQSTIQS 72


>UniRef50_Q5DC94 Cluster: SJCHGC09076 protein; n=1; Schistosoma
           japonicum|Rep: SJCHGC09076 protein - Schistosoma
           japonicum (Blood fluke)
          Length = 109

 Score = 34.3 bits (75), Expect = 2.8
 Identities = 22/64 (34%), Positives = 27/64 (42%)
 Frame = +2

Query: 32  GGARYPIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPN 211
           GGAR PI P      I   +F     GK    P L  L+ +PL P G   K+  P  L  
Sbjct: 39  GGARDPISPKGGPNKISGAAFLKRREGKNPGCPQLNPLEALPLFPGGEKTKKAPPNRLSK 98

Query: 212 SCAP 223
           +  P
Sbjct: 99  NWPP 102


>UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1;
           uncultured bacterium|Rep: Non-ribosomal peptide
           synthetase - uncultured bacterium
          Length = 338

 Score = 33.9 bits (74), Expect = 3.7
 Identities = 18/35 (51%), Positives = 18/35 (51%)
 Frame = -1

Query: 131 WVTPGFSQSRRCKTTASEL*YDSL*GELGTGPPLE 27
           W   GF     C        YDSL GELGTGPPLE
Sbjct: 260 WSKTGFRPF--CLEAGRRAYYDSLYGELGTGPPLE 292


>UniRef50_A6G4K3 Cluster: Putative uncharacterized protein; n=1;
           Plesiocystis pacifica SIR-1|Rep: Putative
           uncharacterized protein - Plesiocystis pacifica SIR-1
          Length = 531

 Score = 32.7 bits (71), Expect = 8.6
 Identities = 15/34 (44%), Positives = 22/34 (64%)
 Frame = +2

Query: 104 VTGKTLALPNLIALQHIPLSPAGVIAKRPAPIAL 205
           V+G  L LP+L+     PL P G++A++  PIAL
Sbjct: 64  VSGPNLQLPHLVDELATPLPPTGLLARKMDPIAL 97


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 670,678,672
Number of Sequences: 1657284
Number of extensions: 13512167
Number of successful extensions: 32306
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 31308
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32296
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 53305790091
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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