Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30074
(726 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 140 1e-33
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 140 1e-33
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 140 1e-33
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.78
At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family prot... 28 5.5
At5g10730.1 68418.m01243 expressed protein 28 7.2
At1g07510.1 68414.m00804 FtsH protease, putative similar to AAA-... 28 7.2
At2g38570.1 68415.m04738 expressed protein ; expression supporte... 27 9.6
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 140 bits (338), Expect = 1e-33
Identities = 63/85 (74%), Positives = 71/85 (83%)
Frame = +2
Query: 251 KVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVA 430
+VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ +
Sbjct: 71 QVDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQ 130
Query: 431 TGPKNVPYLVTHDGRTIRYPDPLIK 505
G K +PYL T+DGRTIRYPDPLIK
Sbjct: 131 FGQKGIPYLNTYDGRTIRYPDPLIK 155
Score = 117 bits (281), Expect = 9e-27
Identities = 52/68 (76%), Positives = 59/68 (86%)
Frame = +3
Query: 42 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 221
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60
Query: 222 VLKIVKQR 245
V+ I+ QR
Sbjct: 61 VISILMQR 68
Score = 109 bits (263), Expect = 1e-24
Identities = 47/80 (58%), Positives = 63/80 (78%)
Frame = +1
Query: 487 PRPTYQSHDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVH 666
P P + +D+I+LD+ KI++FIKF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +H
Sbjct: 150 PDPLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIH 209
Query: 667 IKDSTGHTFATRLNNVFIIG 726
I+DSTGH FATRL NV+ IG
Sbjct: 210 IQDSTGHEFATRLGNVYTIG 229
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 140 bits (338), Expect = 1e-33
Identities = 63/85 (74%), Positives = 71/85 (83%)
Frame = +2
Query: 251 KVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVA 430
+VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ +
Sbjct: 71 QVDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQ 130
Query: 431 TGPKNVPYLVTHDGRTIRYPDPLIK 505
G K +PYL T+DGRTIRYPDPLIK
Sbjct: 131 FGQKGIPYLNTYDGRTIRYPDPLIK 155
Score = 117 bits (281), Expect = 9e-27
Identities = 52/68 (76%), Positives = 59/68 (86%)
Frame = +3
Query: 42 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 221
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60
Query: 222 VLKIVKQR 245
V+ I+ QR
Sbjct: 61 VISILMQR 68
Score = 109 bits (262), Expect = 2e-24
Identities = 47/80 (58%), Positives = 63/80 (78%)
Frame = +1
Query: 487 PRPTYQSHDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVH 666
P P + +D+I+LD+ KI++FIKF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +H
Sbjct: 150 PDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIH 209
Query: 667 IKDSTGHTFATRLNNVFIIG 726
I+DSTGH FATRL NV+ IG
Sbjct: 210 IQDSTGHEFATRLGNVYTIG 229
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 140 bits (338), Expect = 1e-33
Identities = 63/85 (74%), Positives = 71/85 (83%)
Frame = +2
Query: 251 KVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVA 430
+VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ +
Sbjct: 71 QVDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQ 130
Query: 431 TGPKNVPYLVTHDGRTIRYPDPLIK 505
G K +PYL T+DGRTIRYPDPLIK
Sbjct: 131 FGQKGIPYLNTYDGRTIRYPDPLIK 155
Score = 117 bits (281), Expect = 9e-27
Identities = 52/68 (76%), Positives = 59/68 (86%)
Frame = +3
Query: 42 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 221
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60
Query: 222 VLKIVKQR 245
V+ I+ QR
Sbjct: 61 VISILMQR 68
Score = 109 bits (262), Expect = 2e-24
Identities = 47/80 (58%), Positives = 63/80 (78%)
Frame = +1
Query: 487 PRPTYQSHDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVH 666
P P + +D+I+LD+ KI++FIKF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +H
Sbjct: 150 PDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIH 209
Query: 667 IKDSTGHTFATRLNNVFIIG 726
I+DSTGH FATRL NV+ IG
Sbjct: 210 IQDSTGHEFATRLGNVYTIG 229
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.78
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -3
Query: 166 KHSRNLWGPVDGLGAYTPPSLSNIHAL 86
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family protein
similar to lipase [Arabidopsis thaliana] GI:1145627;
contains Pfam profile:PF00657 Lipase/Acylhydrolase with
GDSL-like motif
Length = 323
Score = 28.3 bits (60), Expect = 5.5
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = -3
Query: 289 RISRVSPNFPINFMRRCFTIFRTSFPVK 206
R V+ P NF CF I+ TSFPVK
Sbjct: 166 RAGAVNVVVPGNFPVGCFPIYLTSFPVK 193
>At5g10730.1 68418.m01243 expressed protein
Length = 287
Score = 27.9 bits (59), Expect = 7.2
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +2
Query: 224 PENCETTPHKVDGKVRTDPTYPAGFMDVVSIEKTNE 331
P N E+ KV + TDP +P G +DV I++ ++
Sbjct: 249 PVNVESVA-KVAVRAATDPVFPPGIVDVHGIQRYSQ 283
>At1g07510.1 68414.m00804 FtsH protease, putative similar to
AAA-metalloprotease FtsH [Pisum sativum] GI:15021761;
contains Pfam profiles PF01434: Peptidase family M41,
PF00004: ATPase AAA family
Length = 813
Score = 27.9 bits (59), Expect = 7.2
Identities = 23/87 (26%), Positives = 37/87 (42%)
Frame = +2
Query: 212 RKRSPENCETTPHKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPE 391
R R+P+N E ++VDG + + A F +E +L R + + R TP+
Sbjct: 34 RLRAPQNLEAAVNQVDGGLGFLRRHFASFAARKGLE-AGDLSRAFANPRLRRFFSSQTPK 92
Query: 392 EAKYKLCKVKRVATGPKNVPYLVTHDG 472
+ Y+ K PKN + DG
Sbjct: 93 KKNYENYYPKDSKKAPKNEQKSESRDG 119
>At2g38570.1 68415.m04738 expressed protein ; expression supported
by MPSS
Length = 302
Score = 27.5 bits (58), Expect = 9.6
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +1
Query: 538 TKIMDFIKFESGNLCMITGGRNLGRVGT 621
T I+ +IK G + GGRN+G+V T
Sbjct: 196 TYILVYIKISVGYRVVTPGGRNIGKVST 223
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,243,176
Number of Sequences: 28952
Number of extensions: 384366
Number of successful extensions: 1134
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1074
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1133
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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