BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30072 (656 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 159 4e-40 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 87 3e-18 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 82 8e-17 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 77 2e-15 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 48 1e-06 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 47 3e-06 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 37 0.003 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 3.1 SPAC6G10.10c |||human hmmtag2 homolog|Schizosaccharomyces pombe|... 26 4.2 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 5.5 SPAC637.07 |moe1||translation initiation factor eIF3d Moe1|Schiz... 25 9.6 SPBC2G2.16 |||mannose-6-phosphate isomerase |Schizosaccharomyces... 25 9.6 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 159 bits (385), Expect = 4e-40 Identities = 71/82 (86%), Positives = 73/82 (89%) Frame = +2 Query: 257 MGQKDSYVGDEAXSXRGILTLKYPIEHGIVTNWDDMXKIWHHTXYNELRVAPEXHPVLLT 436 MGQKDSYVGDEA S RGILTLKYPIEHGIV NWDDM KIWHHT YNELRVAPE HP LLT Sbjct: 47 MGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLT 106 Query: 437 EAPLNPKANXEKMTQIMFXTFN 502 EAPLNPK+N EKMTQI+F TFN Sbjct: 107 EAPLNPKSNREKMTQIIFETFN 128 Score = 91.5 bits (217), Expect = 1e-19 Identities = 39/44 (88%), Positives = 43/44 (97%) Frame = +3 Query: 123 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMV 254 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MV Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMV 45 Score = 91.1 bits (216), Expect = 1e-19 Identities = 43/47 (91%), Positives = 45/47 (95%) Frame = +1 Query: 514 YVAIQAVLSLYASGRTTGIVLXSGDGVSHTVPIYEGYALRHAILRLD 654 YVAIQAVLSLYASGRTTGIVL SGDGV+HTVPIYEGYAL HAI+RLD Sbjct: 133 YVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLD 179 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 86.6 bits (205), Expect = 3e-18 Identities = 35/85 (41%), Positives = 58/85 (68%) Frame = +2 Query: 245 RDGRMGQKDSYVGDEAXSXRGILTLKYPIEHGIVTNWDDMXKIWHHTXYNELRVAPEXHP 424 + G + KD VGDEA + R +L +KYP+E+GI+ ++++M ++W +T + +L++ P Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102 Query: 425 VLLTEAPLNPKANXEKMTQIMFXTF 499 +LLTE P+NP AN EKM + MF + Sbjct: 103 ILLTEPPMNPVANREKMCETMFERY 127 Score = 68.1 bits (159), Expect = 1e-12 Identities = 30/48 (62%), Positives = 38/48 (79%) Frame = +1 Query: 511 MYVAIQAVLSLYASGRTTGIVLXSGDGVSHTVPIYEGYALRHAILRLD 654 +YVAIQAVLSLYA G ++G+V+ SGDGV+H VP+YE L H + RLD Sbjct: 132 VYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLD 179 Score = 48.0 bits (109), Expect = 1e-06 Identities = 21/35 (60%), Positives = 26/35 (74%) Frame = +3 Query: 129 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 233 E A +V+DNG+G K G+A D+ PR FPSIVGRP Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 81.8 bits (193), Expect = 8e-17 Identities = 37/81 (45%), Positives = 51/81 (62%), Gaps = 1/81 (1%) Frame = +2 Query: 263 QKDSYVGDEAXSXRGILTLKYPIEHGIVTNWDDMXKIWHHTXYN-ELRVAPEXHPVLLTE 439 QKD +VG EA + RG+L ++ PIE GI+ NW DM +IW + + +L PE HP+LLTE Sbjct: 53 QKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTE 112 Query: 440 APLNPKANXEKMTQIMFXTFN 502 PL N EK+ + + T N Sbjct: 113 PPLANIRNKEKIAEYFYETLN 133 Score = 58.8 bits (136), Expect = 6e-10 Identities = 25/41 (60%), Positives = 35/41 (85%) Frame = +1 Query: 520 AIQAVLSLYASGRTTGIVLXSGDGVSHTVPIYEGYALRHAI 642 ++Q VL+LYAS RTTGIVL GDG++H+VPIY+G+++ AI Sbjct: 140 SLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAI 180 Score = 55.2 bits (127), Expect = 8e-09 Identities = 22/37 (59%), Positives = 29/37 (78%) Frame = +3 Query: 141 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM 251 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVM 48 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 77.0 bits (181), Expect = 2e-15 Identities = 34/79 (43%), Positives = 45/79 (56%), Gaps = 1/79 (1%) Frame = +2 Query: 269 DSYVGDEAXSXRGI-LTLKYPIEHGIVTNWDDMXKIWHHTXYNELRVAPEXHPVLLTEAP 445 D ++G++A +L YPI HG + NWD M + W + + LR PE H LLTE P Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132 Query: 446 LNPKANXEKMTQIMFXTFN 502 LNP N E +IMF +FN Sbjct: 133 LNPPENRENTAEIMFESFN 151 Score = 45.6 bits (103), Expect = 6e-06 Identities = 24/54 (44%), Positives = 35/54 (64%), Gaps = 8/54 (14%) Frame = +1 Query: 505 APMYVAIQAVLSLYASGRT--------TGIVLXSGDGVSHTVPIYEGYALRHAI 642 A +Y+A+QAVL+L AS + TG V+ SGDGV+H +P+ EGY + +I Sbjct: 153 AGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSI 206 Score = 40.7 bits (91), Expect = 2e-04 Identities = 14/28 (50%), Positives = 23/28 (82%) Frame = +3 Query: 141 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 224 +++DNG+G K G+AG+DAP VFP+++ Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 48.0 bits (109), Expect = 1e-06 Identities = 23/87 (26%), Positives = 47/87 (54%), Gaps = 1/87 (1%) Frame = +2 Query: 245 RDGRMGQKDSYVGDEAXSXRGILTL-KYPIEHGIVTNWDDMXKIWHHTXYNELRVAPEXH 421 RD ++ + + VG++ G ++ + P E +++NWD M ++ +T + +L + H Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYT-FLKLGIDRMEH 116 Query: 422 PVLLTEAPLNPKANXEKMTQIMFXTFN 502 P+ +TE NP MT+++F +N Sbjct: 117 PICMTEPLANPTYVRSTMTELLFELYN 143 Score = 35.9 bits (79), Expect = 0.005 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +3 Query: 141 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 236 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 Score = 26.6 bits (56), Expect = 3.1 Identities = 12/35 (34%), Positives = 20/35 (57%), Gaps = 2/35 (5%) Frame = +1 Query: 523 IQAVLSLYASGR--TTGIVLXSGDGVSHTVPIYEG 621 I + S Y + + ++GIVL G+ SH +P+ G Sbjct: 151 IDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNG 185 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 46.8 bits (106), Expect = 3e-06 Identities = 21/60 (35%), Positives = 31/60 (51%) Frame = +2 Query: 311 LTLKYPIEHGIVTNWDDMXKIWHHTXYNELRVAPEXHPVLLTEAPLNPKANXEKMTQIMF 490 + +K I +G V NWD +W + +L+ P HP+L+TE NP N K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +3 Query: 126 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 227 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 Score = 27.5 bits (58), Expect = 1.8 Identities = 13/40 (32%), Positives = 22/40 (55%) Frame = +1 Query: 514 YVAIQAVLSLYASGRTTGIVLXSGDGVSHTVPIYEGYALR 633 Y+A Q + +ASG+ T ++ G S IY+G+ L+ Sbjct: 131 YLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQ 170 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 36.7 bits (81), Expect = 0.003 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +3 Query: 126 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 227 EE+ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 Score = 35.5 bits (78), Expect = 0.007 Identities = 18/77 (23%), Positives = 38/77 (49%), Gaps = 2/77 (2%) Frame = +2 Query: 266 KDSYVGDEAXSXRGI--LTLKYPIEHGIVTNWDDMXKIWHHTXYNELRVAPEXHPVLLTE 439 ++ YV DE I + +K +GI+ +W+ W +L+V P + +++TE Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108 Query: 440 APLNPKANXEKMTQIMF 490 NP++ +++ + F Sbjct: 109 PSWNPQSVRQQIMEAAF 125 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 26.6 bits (56), Expect = 3.1 Identities = 12/32 (37%), Positives = 18/32 (56%), Gaps = 1/32 (3%) Frame = +3 Query: 171 KAGFAGDDAP-RAVFPSIVGRPRHQGVMVVWD 263 KA F+G R+ + VG+P H G++ WD Sbjct: 1533 KAKFSGQKGTLRSKYSLEVGKPMHYGIISEWD 1564 >SPAC6G10.10c |||human hmmtag2 homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 194 Score = 26.2 bits (55), Expect = 4.2 Identities = 19/41 (46%), Positives = 21/41 (51%) Frame = +3 Query: 498 STXPHVRRHPSRALAVRVRSYHRYRAGLRRRCLPHRAHLRR 620 ST P RH R R RS HR+ G RR P+R HL R Sbjct: 128 STSP-ANRH-RRKEKERTRSNHRH--GSHRRHEPYRTHLSR 164 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 5.5 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -3 Query: 168 TYRSHCLLPTRQLLRRTS*ISEI 100 TYR H +PT LL T +SEI Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190 >SPAC637.07 |moe1||translation initiation factor eIF3d Moe1|Schizosaccharomyces pombe|chr 1|||Manual Length = 567 Score = 25.0 bits (52), Expect = 9.6 Identities = 10/21 (47%), Positives = 10/21 (47%) Frame = -3 Query: 609 GHGVGDTVAGXQHDTGGTTGR 547 G G GDTV GG GR Sbjct: 139 GRGAGDTVVSRSSGAGGARGR 159 >SPBC2G2.16 |||mannose-6-phosphate isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 412 Score = 25.0 bits (52), Expect = 9.6 Identities = 12/43 (27%), Positives = 21/43 (48%) Frame = +3 Query: 510 HVRRHPSRALAVRVRSYHRYRAGLRRRCLPHRAHLRRIRTPPR 638 +VR HP A+ +R H Y +G C+ ++ R+ P+ Sbjct: 248 YVRLHPGEAVFLRALDPHAYVSGNIIECMAASDNVIRLGFTPK 290 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,642,976 Number of Sequences: 5004 Number of extensions: 51629 Number of successful extensions: 153 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 123 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 150 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 297805304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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