BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30060 (814 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g45540.1 68415.m05663 WD-40 repeat family protein / beige-rel... 32 0.52 At5g42370.1 68418.m05159 expressed protein 30 2.1 At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to ... 29 4.9 At5g19700.1 68418.m02343 MATE efflux protein-related contains Pf... 29 4.9 At2g19150.1 68415.m02235 pectinesterase family protein contains ... 29 4.9 At3g29033.1 68416.m03629 glycine-rich protein 28 6.4 At2g03340.1 68415.m00293 WRKY family transcription factor contai... 28 6.4 At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox... 28 6.4 At5g32613.1 68418.m03881 zinc knuckle (CCHC-type) family protein... 28 8.5 At2g35890.1 68415.m04406 calcium-dependent protein kinase, putat... 28 8.5 >At2g45540.1 68415.m05663 WD-40 repeat family protein / beige-related contains Pfam PF02138: Beige/BEACH domain; contains Pfam PF00400: WD domain, G-beta repeat (3 copies) Length = 2946 Score = 31.9 bits (69), Expect = 0.52 Identities = 16/32 (50%), Positives = 21/32 (65%), Gaps = 1/32 (3%) Frame = +1 Query: 124 VTGTCDAKFLIW-YH*AVTSRTCATESAEGSG 216 VTG+ D+ L+W H A TSRT +E + GSG Sbjct: 2684 VTGSRDSTVLLWRIHKAFTSRTSVSEPSTGSG 2715 >At5g42370.1 68418.m05159 expressed protein Length = 447 Score = 29.9 bits (64), Expect = 2.1 Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = -2 Query: 609 PFYRIPWNSNAQAEKKTLPGPLGGVFRPL-WVTPSTL 502 P Y + + Q+ +K +P PL + R L W TPST+ Sbjct: 285 PLYDVTSSGLVQSVEKVVPRPLRSIVRLLFWYTPSTM 321 >At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to autophagy 7 [Arabidopsis thaliana] GI:19912147; contains Pfam profile PF00899: ThiF family Length = 697 Score = 28.7 bits (61), Expect = 4.9 Identities = 13/45 (28%), Positives = 25/45 (55%) Frame = -1 Query: 670 PQRVSGHRRKCGALRVPNHISLL*DSMELERSGRKENSSRTSRRR 536 P ++G CG +V NH++LL +S+ L+ ++S +R + Sbjct: 42 PISITGFYGPCGHPQVSNHLTLLSESLPLDEQSLIASTSHGNRNK 86 >At5g19700.1 68418.m02343 MATE efflux protein-related contains Pfam profile PF01554: Uncharacterized membrane protein family Length = 508 Score = 28.7 bits (61), Expect = 4.9 Identities = 11/30 (36%), Positives = 19/30 (63%) Frame = -2 Query: 546 LGGVFRPLWVTPSTLVFKDEGTIIETVPLP 457 + G+ +P W PS+ FKD G ++ T+ +P Sbjct: 239 IAGLHQPTWTRPSSECFKDWGPVV-TLAIP 267 >At2g19150.1 68415.m02235 pectinesterase family protein contains Pfam profile: PF01095 pectinesterase Length = 339 Score = 28.7 bits (61), Expect = 4.9 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = +1 Query: 640 ICGGVRILSADLENSGEGCTWRCRAGSYPYPQQV 741 I GG+ + ++ EGCT + R G YP P +V Sbjct: 185 ISGGIDFIFGGAQSIFEGCTLKLRVGIYP-PNEV 217 >At3g29033.1 68416.m03629 glycine-rich protein Length = 167 Score = 28.3 bits (60), Expect = 6.4 Identities = 16/47 (34%), Positives = 21/47 (44%) Frame = -2 Query: 258 YPKICTDGGSKRLTPRPFCALRRARPTRYGLMIPN*KFSITRAGNGS 118 Y I D S+ +P P P RYG + PN +F +AG S Sbjct: 12 YGFIAGDDDSQSSSPSPGHGQNSPSPRRYGQVTPNPRFHGYQAGGSS 58 >At2g03340.1 68415.m00293 WRKY family transcription factor contains Pfam profile: PF03106 WRKY DNA -binding domain Length = 513 Score = 28.3 bits (60), Expect = 6.4 Identities = 14/29 (48%), Positives = 19/29 (65%) Frame = +2 Query: 485 PSSLKTSVDGVTQSGLKTPPRGPGRVFFS 571 PS LK+S GV++ + PPR G +FFS Sbjct: 9 PSKLKSST-GVSRPTISLPPRPFGEMFFS 36 >At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140 [Lycopersicon esculentum], GB:CAB56692 [Arabidopsis thaliana] Length = 926 Score = 28.3 bits (60), Expect = 6.4 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = -3 Query: 665 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQK 567 +S + P R ++PYLPS G RTLR+K Sbjct: 215 QSQKDHPSKRILFTNQPYLPSETPSGLRTLREK 247 >At5g32613.1 68418.m03881 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 457 Score = 27.9 bits (59), Expect = 8.5 Identities = 15/40 (37%), Positives = 22/40 (55%) Frame = -2 Query: 582 NAQAEKKTLPGPLGGVFRPLWVTPSTLVFKDEGTIIETVP 463 N+ ++ T P P PLW+TPST F ++GT + P Sbjct: 145 NSSFDRITNPSPTSSK-GPLWMTPST--FLEDGTPMVVAP 181 >At2g35890.1 68415.m04406 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium-dependent protein kinase, isoform AK1 (CDPK). [Arabidopsis thaliana] SWISS-PROT:Q06850; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 520 Score = 27.9 bits (59), Expect = 8.5 Identities = 11/36 (30%), Positives = 18/36 (50%) Frame = +1 Query: 4 MTAGRWPWKSESAKECATTHLPKQPALKMDGAEAFC 111 +T+ WP SESAK+ L + P ++ + C Sbjct: 351 LTSDPWPQVSESAKDLIRKMLERNPIQRLTAQQVLC 386 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,986,102 Number of Sequences: 28952 Number of extensions: 433396 Number of successful extensions: 1241 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 1179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1240 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1853336000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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