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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30048
         (303 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g03670.1 68417.m00502 hypothetical protein                          27   2.4  
At2g41990.1 68415.m05194 expressed protein                             27   2.4  
At2g21910.1 68415.m02603 cytochrome P450, putative                     26   5.6  
At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identic...    25   9.7  
At2g41220.1 68415.m05090 glutamate synthase, chloroplast (GLU2) ...    25   9.7  

>At4g03670.1 68417.m00502 hypothetical protein
          Length = 171

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
 Frame = -3

Query: 274 KLWNNSSASLYYSWRESVNFST-MLKFTRLYADH-NMNFL 161
           +LW      L Y W   +N +T +L+F ++ ADH  +NFL
Sbjct: 126 RLWRELGLDLGYEWTFGMNTTTCVLRFWKV-ADHEGLNFL 164


>At2g41990.1 68415.m05194 expressed protein 
          Length = 297

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 9/17 (52%), Positives = 13/17 (76%)
 Frame = +1

Query: 55  CSGLHHDRDCGTSRFTN 105
           CS +HH R+  TSRF++
Sbjct: 61  CSPIHHSRESSTSRFSD 77


>At2g21910.1 68415.m02603 cytochrome P450, putative 
          Length = 510

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 13/47 (27%), Positives = 26/47 (55%)
 Frame = -1

Query: 303 FFFFFFPLNKSYGTIQARHFTILGAKVLISLLC*NLLDFMLIII*IS 163
           FFFF F ++K    I  R++ +LG    + ++   + D++  I+ +S
Sbjct: 16  FFFFHFLIHKKSHQITPRNWPVLGMLPGVLVMLHRINDYVAEILEVS 62


>At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identical
           to AtSPO11-1 [Arabidopsis thaliana] GI:13383478;
           contains Pfam profile PF04406: Type IIB DNA
           topoisomerase; identical to cDNA putative topoisomerase
           VIA (SPO11 gene 1) GI:7270974
          Length = 362

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 12/34 (35%), Positives = 17/34 (50%)
 Frame = +1

Query: 1   LNSSHFRLYCMGKDV*CFCSGLHHDRDCGTSRFT 102
           ++ + FR YCM  +  C CS    D+  G   FT
Sbjct: 36  ISINQFRNYCMNPEADCLCSS---DKPKGQEIFT 66


>At2g41220.1 68415.m05090 glutamate synthase, chloroplast (GLU2) /
            ferredoxin-dependent glutamate synthase (Fd-GOGAT 2)
            identical to SP|Q9T0P4 Ferredoxin-dependent glutamate
            synthase 2, chloroplast precursor (EC 1.4.7.1) (Fd-GOGAT
            2) {Arabidopsis thaliana}
          Length = 1629

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 10/21 (47%), Positives = 14/21 (66%)
 Frame = -3

Query: 73   RDVIHYKSIRHPFPCNKDESA 11
            RD++ +KS R+P P  K E A
Sbjct: 946  RDLLEFKSDRNPIPVGKVEPA 966


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,624,742
Number of Sequences: 28952
Number of extensions: 90142
Number of successful extensions: 150
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 12,070,560
effective HSP length: 70
effective length of database: 10,043,920
effective search space used: 301317600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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