Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30019
(573 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ... 82 1e-14
UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ... 82 1e-14
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L... 82 1e-14
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet... 82 1e-14
UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo... 81 2e-14
UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:... 56 4e-07
UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1; ... 49 9e-05
UniRef50_P06219 Cluster: Beta-galactosidase; n=11; Gammaproteoba... 48 2e-04
UniRef50_P81650 Cluster: Beta-galactosidase; n=26; Gammaproteoba... 39 0.072
UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia sp... 39 0.096
UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3; ... 37 0.29
UniRef50_Q5DC94 Cluster: SJCHGC09076 protein; n=1; Schistosoma j... 37 0.39
UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus la... 36 0.68
UniRef50_A0UVE2 Cluster: Glycoside hydrolase family 2, TIM barre... 36 0.89
UniRef50_A0D095 Cluster: Chromosome undetermined scaffold_33, wh... 34 2.1
UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1; ... 34 2.7
UniRef50_A0M224 Cluster: Beta-galactosidase; n=1; Gramella forse... 34 2.7
UniRef50_A6FJQ2 Cluster: 50S ribosomal protein L5; n=8; Bacteria... 33 3.6
UniRef50_Q15XN9 Cluster: Glycoside hydrolase family 2, TIM barre... 33 6.3
UniRef50_Q2R308 Cluster: Putative uncharacterized protein; n=2; ... 33 6.3
UniRef50_UPI0000F1EDC6 Cluster: PREDICTED: hypothetical protein;... 32 8.3
UniRef50_UPI000038DE68 Cluster: COG0457: FOG: TPR repeat; n=1; N... 32 8.3
UniRef50_Q4S5F6 Cluster: Chromosome 19 SCAF14731, whole genome s... 32 8.3
>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
Erwinia amylovora|Rep: Putative uncharacterized protein
- Erwinia amylovora (Fire blight bacteria)
Length = 123
Score = 81.8 bits (193), Expect = 1e-14
Identities = 36/36 (100%), Positives = 36/36 (100%)
Frame = +2
Query: 146 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
Sbjct: 68 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 103
Score = 35.5 bits (78), Expect = 0.89
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = +1
Query: 256 TDRPSQQLRSLNGEWQ 303
TDRPSQQLR LNGEW+
Sbjct: 105 TDRPSQQLRXLNGEWR 120
>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
organisms|Rep: LacZ-alpha peptide - Escherichia coli
Length = 90
Score = 81.8 bits (193), Expect = 1e-14
Identities = 36/36 (100%), Positives = 36/36 (100%)
Frame = +2
Query: 146 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
Sbjct: 22 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 57
>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
protein - Phage M13mp18
Length = 102
Score = 81.8 bits (193), Expect = 1e-14
Identities = 36/36 (100%), Positives = 36/36 (100%)
Frame = +2
Query: 146 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
Sbjct: 26 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 61
Score = 37.5 bits (83), Expect = 0.22
Identities = 15/16 (93%), Positives = 16/16 (100%)
Frame = +1
Query: 256 TDRPSQQLRSLNGEWQ 303
TDRPSQQLRSLNGEW+
Sbjct: 63 TDRPSQQLRSLNGEWR 78
>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
Beta-galactosidase - Escherichia coli (strain K12)
Length = 1024
Score = 81.8 bits (193), Expect = 1e-14
Identities = 36/36 (100%), Positives = 36/36 (100%)
Frame = +2
Query: 146 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
Sbjct: 8 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 43
Score = 37.5 bits (83), Expect = 0.22
Identities = 15/16 (93%), Positives = 16/16 (100%)
Frame = +1
Query: 256 TDRPSQQLRSLNGEWQ 303
TDRPSQQLRSLNGEW+
Sbjct: 45 TDRPSQQLRSLNGEWR 60
>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
Eukaryota|Rep: beta-galactosidase - Entamoeba
histolytica HM-1:IMSS
Length = 86
Score = 81.0 bits (191), Expect = 2e-14
Identities = 38/54 (70%), Positives = 42/54 (77%), Gaps = 1/54 (1%)
Frame = +3
Query: 144 HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPHRS-PFPTVAQPEWRMA 302
HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVI++ P + +WRMA
Sbjct: 5 HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVISEEARTDRPSQQLRSLKWRMA 58
>UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:
Beta-galactosidase - Yersinia pseudotuberculosis
Length = 1066
Score = 56.4 bits (130), Expect = 4e-07
Identities = 23/36 (63%), Positives = 27/36 (75%)
Frame = +2
Query: 146 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
L +L RRDWENP +TQ +RL AHPPF SWR+ E A
Sbjct: 15 LPQILSRRDWENPQITQYHRLEAHPPFHSWRDVESA 50
>UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1;
Enterobacter sakazakii ATCC BAA-894|Rep: Putative
uncharacterized protein - Enterobacter sakazakii ATCC
BAA-894
Length = 1043
Score = 48.8 bits (111), Expect = 9e-05
Identities = 17/36 (47%), Positives = 25/36 (69%)
Frame = +2
Query: 146 LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
LA +L R DW+NP +T +NRL +H P WR+++ A
Sbjct: 18 LATILARNDWQNPAITSVNRLPSHTPLHGWRDADRA 53
>UniRef50_P06219 Cluster: Beta-galactosidase; n=11;
Gammaproteobacteria|Rep: Beta-galactosidase - Klebsiella
pneumoniae
Length = 1034
Score = 48.0 bits (109), Expect = 2e-04
Identities = 24/41 (58%), Positives = 27/41 (65%)
Frame = +2
Query: 155 VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAPPIALPNS 277
VL R DW N +T LNRL AHP FASWR+ E A LP+S
Sbjct: 17 VLAREDWHNQTITHLNRLPAHPVFASWRD-ELAARDNLPSS 56
>UniRef50_P81650 Cluster: Beta-galactosidase; n=26;
Gammaproteobacteria|Rep: Beta-galactosidase -
Pseudoalteromonas haloplanktis (Alteromonas
haloplanktis)
Length = 1039
Score = 39.1 bits (87), Expect = 0.072
Identities = 14/33 (42%), Positives = 22/33 (66%)
Frame = +2
Query: 155 VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
++ RRDWENP Q+N++ AH P ++ E+A
Sbjct: 7 IINRRDWENPITVQVNQVKAHSPLNGFKTIEDA 39
>UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia
spumigena CCY 9414|Rep: Beta-D-galactosidase - Nodularia
spumigena CCY 9414
Length = 72
Score = 38.7 bits (86), Expect = 0.096
Identities = 15/18 (83%), Positives = 18/18 (100%)
Frame = +1
Query: 256 TDRPSQQLRSLNGEWQIV 309
TDRPSQQLRSLNGEW+++
Sbjct: 55 TDRPSQQLRSLNGEWRLM 72
>UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3;
Plasmodium (Vinckeia)|Rep: Putative uncharacterized
protein - Plasmodium berghei
Length = 275
Score = 37.1 bits (82), Expect = 0.29
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +3
Query: 93 RGGARYPIRPIVSRIT 140
RGGARYPIRPIVSRIT
Sbjct: 260 RGGARYPIRPIVSRIT 275
>UniRef50_Q5DC94 Cluster: SJCHGC09076 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC09076 protein - Schistosoma
japonicum (Blood fluke)
Length = 109
Score = 36.7 bits (81), Expect = 0.39
Identities = 17/37 (45%), Positives = 23/37 (62%)
Frame = +2
Query: 149 AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAPP 259
A L+RR+ +NPG QLN L A P F +++APP
Sbjct: 57 AAFLKRREGKNPGCPQLNPLEALPLFPGGEKTKKAPP 93
>UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus
lactis|Rep: Beta-galactosidase - Lactococcus lactis
subsp. lactis (Streptococcus lactis)
Length = 998
Score = 35.9 bits (79), Expect = 0.68
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = +2
Query: 155 VLQRRDWENPGVTQLNRLAAHPP 223
VL+R+DWENP V+ NRL H P
Sbjct: 9 VLERKDWENPVVSNWNRLPMHTP 31
>UniRef50_A0UVE2 Cluster: Glycoside hydrolase family 2, TIM barrel;
n=1; Clostridium cellulolyticum H10|Rep: Glycoside
hydrolase family 2, TIM barrel - Clostridium
cellulolyticum H10
Length = 1033
Score = 35.5 bits (78), Expect = 0.89
Identities = 12/29 (41%), Positives = 20/29 (68%)
Frame = +2
Query: 167 RDWENPGVTQLNRLAAHPPFASWRNSEEA 253
R+WEN +TQ+NR H P+ ++ + E+A
Sbjct: 3 REWENQYITQINRYPMHSPYGAYESVEQA 31
>UniRef50_A0D095 Cluster: Chromosome undetermined scaffold_33, whole
genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_33,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 1173
Score = 34.3 bits (75), Expect = 2.1
Identities = 15/44 (34%), Positives = 26/44 (59%)
Frame = -2
Query: 416 VYSFDL*GILPISAYWLKNELI*QKFNANFNKILTLTICHSPFR 285
+++F L G L +WLKN+ KF++ F ++L L + + FR
Sbjct: 665 IFNFSLQGALSYIDFWLKNQHFDDKFSSTFTQLLLLALGVTVFR 708
>UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1;
uncultured bacterium|Rep: Non-ribosomal peptide
synthetase - uncultured bacterium
Length = 338
Score = 33.9 bits (74), Expect = 2.7
Identities = 18/35 (51%), Positives = 18/35 (51%)
Frame = -1
Query: 195 WVTPGFSQSRRCKTTASEL*YDSL*GELGTGPPLE 91
W GF C YDSL GELGTGPPLE
Sbjct: 260 WSKTGFRPF--CLEAGRRAYYDSLYGELGTGPPLE 292
>UniRef50_A0M224 Cluster: Beta-galactosidase; n=1; Gramella forsetii
KT0803|Rep: Beta-galactosidase - Gramella forsetii
(strain KT0803)
Length = 1049
Score = 33.9 bits (74), Expect = 2.7
Identities = 17/44 (38%), Positives = 22/44 (50%)
Frame = +2
Query: 170 DWENPGVTQLNRLAAHPPFASWRNSEEAPPIALPNSCAA*MANG 301
DWENP VT +N+L A S+ N + A + NS NG
Sbjct: 26 DWENPAVTGINKLPARATMYSFSNKQAAINLNKENSDRVKSLNG 69
>UniRef50_A6FJQ2 Cluster: 50S ribosomal protein L5; n=8;
Bacteria|Rep: 50S ribosomal protein L5 - Moritella sp.
PE36
Length = 45
Score = 33.5 bits (73), Expect = 3.6
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = -1
Query: 309 YNLPFAIQAAQLLGRAIG 256
+ PFAIQAAQLLGRAIG
Sbjct: 8 HQAPFAIQAAQLLGRAIG 25
>UniRef50_Q15XN9 Cluster: Glycoside hydrolase family 2, TIM barrel
precursor; n=1; Pseudoalteromonas atlantica T6c|Rep:
Glycoside hydrolase family 2, TIM barrel precursor -
Pseudoalteromonas atlantica (strain T6c / BAA-1087)
Length = 1079
Score = 32.7 bits (71), Expect = 6.3
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +2
Query: 164 RRDWENPGVTQLNRLAAHPPFASWRNSEEA 253
+ DWENP V Q+NRL A S+ E+A
Sbjct: 31 KNDWENPDVIQINRLPARATSYSFDTPEQA 60
>UniRef50_Q2R308 Cluster: Putative uncharacterized protein; n=2;
Oryza sativa|Rep: Putative uncharacterized protein -
Oryza sativa subsp. japonica (Rice)
Length = 103
Score = 32.7 bits (71), Expect = 6.3
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -2
Query: 293 PFRLRNCWEGRSVGPLRY 240
PFR R CW R+VGP R+
Sbjct: 35 PFRSRTCWHSRAVGPTRF 52
>UniRef50_UPI0000F1EDC6 Cluster: PREDICTED: hypothetical protein;
n=1; Danio rerio|Rep: PREDICTED: hypothetical protein -
Danio rerio
Length = 195
Score = 32.3 bits (70), Expect = 8.3
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +2
Query: 146 LAVVLQRRDWENP 184
LAVVLQRRDWENP
Sbjct: 179 LAVVLQRRDWENP 191
>UniRef50_UPI000038DE68 Cluster: COG0457: FOG: TPR repeat; n=1;
Nostoc punctiforme PCC 73102|Rep: COG0457: FOG: TPR
repeat - Nostoc punctiforme PCC 73102
Length = 532
Score = 32.3 bits (70), Expect = 8.3
Identities = 14/37 (37%), Positives = 23/37 (62%), Gaps = 1/37 (2%)
Frame = +2
Query: 146 LAVVLQRRDWENPGVTQLNRLAAH-PPFASWRNSEEA 253
+ V+L+ DWE P + QL+ L ++ P SW + +EA
Sbjct: 96 IPVLLRYADWETPPIDQLSPLPSNRKPIKSWNDRDEA 132
>UniRef50_Q4S5F6 Cluster: Chromosome 19 SCAF14731, whole genome
shotgun sequence; n=2; Clupeocephala|Rep: Chromosome 19
SCAF14731, whole genome shotgun sequence - Tetraodon
nigroviridis (Green puffer)
Length = 842
Score = 32.3 bits (70), Expect = 8.3
Identities = 21/58 (36%), Positives = 29/58 (50%), Gaps = 3/58 (5%)
Frame = +3
Query: 138 TIHWPSFYNVVTGKTLALPNLIALQHIPLSPAG---VIAKRPHRSPFPTVAQPEWRMA 302
++H S Y + T AL L + H+ LSP+ V K+ SPFP V QP +A
Sbjct: 755 SLHPSSLYKTPSPSTPALSPLSSSSHLSLSPSSPGDVPPKQQVFSPFPCVKQPRKSVA 812
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 555,114,538
Number of Sequences: 1657284
Number of extensions: 11578093
Number of successful extensions: 28601
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 27840
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28597
length of database: 575,637,011
effective HSP length: 96
effective length of database: 416,537,747
effective search space used: 39154548218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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