Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30014
(383 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70... 70 5e-13
At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, puta... 68 2e-12
At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1 (HS... 65 1e-11
At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2 (HS... 65 1e-11
At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3 (HS... 65 2e-11
At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70... 64 4e-11
At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)... 58 2e-09
At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)... 58 2e-09
At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)... 58 2e-09
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)... 52 1e-07
At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp... 31 0.26
At1g14640.1 68414.m01740 SWAP (Suppressor-of-White-APricot)/surp... 29 1.4
At5g01570.1 68418.m00072 hypothetical protein hypothetical prote... 28 2.5
At3g56850.1 68416.m06322 ABA-responsive element-binding protein ... 28 2.5
At2g01270.1 68415.m00040 thioredoxin family protein low similari... 27 3.3
At3g19050.1 68416.m02420 kinesin motor protein-related contains ... 27 4.3
At2g44710.1 68415.m05564 RNA recognition motif (RRM)-containing ... 27 4.3
At2g41070.3 68415.m05073 basic leucine zipper transcription fact... 27 4.3
At2g41070.2 68415.m05072 basic leucine zipper transcription fact... 27 4.3
At2g41070.1 68415.m05071 basic leucine zipper transcription fact... 27 4.3
At4g29790.1 68417.m04242 expressed protein 26 9.9
At3g62240.1 68416.m06992 zinc finger (C2H2 type) family protein ... 26 9.9
At3g60000.1 68416.m06699 hypothetical protein contains Pfam pro... 26 9.9
At3g16565.1 68416.m02118 alanyl-tRNA synthetase-related low simi... 26 9.9
At2g37870.1 68415.m04649 protease inhibitor/seed storage/lipid t... 26 9.9
>At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70,
putative similar to heat shock protein hsp70 GI:1771478
from [Pisum sativum]
Length = 646
Score = 70.1 bits (164), Expect = 5e-13
Identities = 30/55 (54%), Positives = 43/55 (78%)
Frame = +1
Query: 1 LKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
L +K++ DKQ I ++TI+W++ NQLA+ +E+E+K KELEGI NPII+KMYQ
Sbjct: 563 LAQKLTQEDKQKIEKAIDETIEWIEGNQLAEVDEFEYKLKELEGICNPIISKMYQ 617
>At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein,
putative / HSC70, putative / HSP70, putative strong
similarity to heat shock cognate 70 kd protein 1
SP:P22953 [Arabidopsis thaliana (Mouse-ear cress)]
Length = 617
Score = 67.7 bits (158), Expect = 2e-12
Identities = 28/53 (52%), Positives = 39/53 (73%)
Frame = +1
Query: 7 EKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
EK+ +DK+ D + I+WLD NQLA+ +E+EHK KELE +++ IITKMYQ
Sbjct: 564 EKLPAADKKKFEDSIEEVIQWLDDNQLAEADEFEHKMKELESVWSTIITKMYQ 616
>At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1
(HSC70-1) (HSP70-1) identical to SP|P22953 Heat shock
cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
thaliana}
Length = 651
Score = 65.3 bits (152), Expect = 1e-11
Identities = 29/53 (54%), Positives = 37/53 (69%)
Frame = +1
Query: 7 EKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
EK+ +DK+ I D I+WL+ NQLA+ +E+E K KELE I NPII KMYQ
Sbjct: 566 EKLPAADKKKIEDSIEQAIQWLEGNQLAEADEFEDKMKELESICNPIIAKMYQ 618
>At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2
(HSC70-2) (HSP70-2) identical to SP|P22954 Heat shock
cognate 70 kDa protein 2 (Hsc70.2) {Arabidopsis
thaliana}
Length = 653
Score = 65.3 bits (152), Expect = 1e-11
Identities = 27/53 (50%), Positives = 37/53 (69%)
Frame = +1
Query: 7 EKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
EK+ +DK+ + D + I+WLD NQL + +E+E K KELE + NPII KMYQ
Sbjct: 566 EKLPAADKKKVEDSIEEAIQWLDGNQLGEADEFEDKMKELESVCNPIIAKMYQ 618
>At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3
(HSC70-3) (HSP70-3) identical to SP|O65719 Heat shock
cognate 70 kDa protein 3 (Hsc70.3) {Arabidopsis
thaliana}
Length = 649
Score = 64.9 bits (151), Expect = 2e-11
Identities = 29/53 (54%), Positives = 38/53 (71%)
Frame = +1
Query: 7 EKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
EK++ DK+ I D I+WL++NQLA+ +E+E K KELE I NPII KMYQ
Sbjct: 566 EKLAGDDKKKIEDSIEAAIEWLEANQLAECDEFEDKMKELESICNPIIAKMYQ 618
>At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein
GI:425194 [Spinacia oleracea]
Length = 650
Score = 63.7 bits (148), Expect = 4e-11
Identities = 27/55 (49%), Positives = 38/55 (69%)
Frame = +1
Query: 1 LKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
+ K+ +DK+ I D + I+WLD NQLA+ +E+E K KELE + NPII +MYQ
Sbjct: 564 IASKLDAADKKKIEDAIDQAIEWLDGNQLAEADEFEDKMKELESLCNPIIARMYQ 618
>At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 613
Score = 58.4 bits (135), Expect = 2e-09
Identities = 24/55 (43%), Positives = 37/55 (67%)
Frame = +1
Query: 1 LKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
L +K+ +K+ I + ++WLD NQ ++KEEY+ K KE+E + NPIIT +YQ
Sbjct: 535 LADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 589
>At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 668
Score = 58.4 bits (135), Expect = 2e-09
Identities = 24/55 (43%), Positives = 37/55 (67%)
Frame = +1
Query: 1 LKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
L +K+ +K+ I + ++WLD NQ ++KEEY+ K KE+E + NPIIT +YQ
Sbjct: 590 LADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 644
>At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)
SWISS-PROT:Q9LKR3 PMID:8888624
Length = 669
Score = 58.4 bits (135), Expect = 2e-09
Identities = 24/55 (43%), Positives = 37/55 (67%)
Frame = +1
Query: 1 LKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
L +K+ +K+ I + ++WLD NQ ++KEEY+ K KE+E + NPIIT +YQ
Sbjct: 590 LADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 644
>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
Similar to Arabidopsis luminal binding protein
(gb|D89342); contains Pfam domain PF00012: dnaK protein
Length = 678
Score = 52.0 bits (119), Expect = 1e-07
Identities = 21/55 (38%), Positives = 37/55 (67%)
Frame = +1
Query: 1 LKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
L +KISD DK+ + + ++WL+ N A+KE+Y+ K KE+E + +P+I +Y+
Sbjct: 604 LAKKISDEDKEKMEGVLKEALEWLEENVNAEKEDYDEKLKEVELVCDPVIKSVYE 658
>At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein / ubiquitin family protein
similar to SP|Q15459 Splicing factor 3 subunit 1
(Spliceosome associated protein 114) {Homo sapiens};
contains Pfam profiles PF00240: Ubiquitin family,
PF01805: Surp module
Length = 785
Score = 31.1 bits (67), Expect = 0.26
Identities = 19/52 (36%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
Frame = +1
Query: 1 LKEKI--SDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPII 150
LKEK+ S +D T+L++C ++W D +Q K++ E +KELE + +I
Sbjct: 252 LKEKLRKSAADLTTVLERCLHRLEW-DRSQEQQKKK-EEDEKELERVQMAMI 301
>At1g14640.1 68414.m01740 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein similar to human splicing
factor GB:CAA59494 GI:899298 from [Homo sapiens];
contains Pfam profile PF01805: Surp module
Length = 735
Score = 28.7 bits (61), Expect = 1.4
Identities = 14/43 (32%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +1
Query: 1 LKEKISDS--DKQTILDKCNDTIKWLDSNQLADKEEYEHKQKE 123
LKEK+ S D T+L++C + ++W + +E + K+KE
Sbjct: 243 LKEKLRKSVADLTTVLERCLNRLEWDRFQEEEKNKEEDEKEKE 285
>At5g01570.1 68418.m00072 hypothetical protein hypothetical protein
T16O11.19 - Arabidopsis thaliana, EMBL:AC010871
Length = 157
Score = 27.9 bits (59), Expect = 2.5
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = +1
Query: 25 DKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELE 129
D Q LD C + +S ++AD+EE E QKEL+
Sbjct: 58 DLQKKLDVCKEKTDEANS-EIADEEEIERLQKELD 91
>At3g56850.1 68416.m06322 ABA-responsive element-binding protein 3
(AREB3) identical to ABA-responsive element binding
protein 3 (AREB3) [Arabidopsis thaliana] GI:9967421
Length = 297
Score = 27.9 bits (59), Expect = 2.5
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +2
Query: 95 RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 238
R S + SR +A+T + +R+ EE+ + + E P VPPP
Sbjct: 236 RESAARSRARKQAYTHELEIKVSRLEEENERLRKQKEVEKILPSVPPP 283
>At2g01270.1 68415.m00040 thioredoxin family protein low similarity
to quiescin [Homo sapiens] GI:13257405; contains Pfam
profiles PF00085: Thioredoxin, PF04777: Erv1 / Alr
family
Length = 495
Score = 27.5 bits (58), Expect = 3.3
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +1
Query: 67 WLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
+L SNQ + + ++EH K L+ Y P + +Y+
Sbjct: 402 YLSSNQKSIEWDHEHVYKFLKNYYGPKLVSLYK 434
>At3g19050.1 68416.m02420 kinesin motor protein-related contains Pfam
profile: PF00225 Kinesin motor domain; contains
non-consensus splice site (GC) at intron 12
Length = 2722
Score = 27.1 bits (57), Expect = 4.3
Identities = 13/38 (34%), Positives = 23/38 (60%)
Frame = +1
Query: 22 SDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGI 135
+D++T + + ++ I L+ + A EY HK KELE +
Sbjct: 2336 ADQKTEITQLSEHISELNLHAEAQASEYMHKFKELEAM 2373
>At2g44710.1 68415.m05564 RNA recognition motif (RRM)-containing
protein
Length = 809
Score = 27.1 bits (57), Expect = 4.3
Identities = 10/26 (38%), Positives = 13/26 (50%)
Frame = +2
Query: 161 TRVPEESPEVCRASRAEHPEPEVPPP 238
+R P S + SR P P +PPP
Sbjct: 521 SRAPSSSAKRASGSRGRRPRPPLPPP 546
>At2g41070.3 68415.m05073 basic leucine zipper transcription factor
(BZIP12) nearly identical to basic leucine zipper
transcription factor [Arabidopsis thaliana] GI:21694632;
contains a bZIP transcription factor basic domain
signature (PDOC00036)
Length = 262
Score = 27.1 bits (57), Expect = 4.3
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +2
Query: 95 RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 238
R S + SR +A+T + +R+ EE+ ++ R E P PPP
Sbjct: 201 RESAARSRARKQAYTHELEIKVSRLEEENEKLRRLKEVEKILPSEPPP 248
>At2g41070.2 68415.m05072 basic leucine zipper transcription factor
(BZIP12) nearly identical to basic leucine zipper
transcription factor [Arabidopsis thaliana] GI:21694632;
contains a bZIP transcription factor basic domain
signature (PDOC00036)
Length = 262
Score = 27.1 bits (57), Expect = 4.3
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +2
Query: 95 RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 238
R S + SR +A+T + +R+ EE+ ++ R E P PPP
Sbjct: 201 RESAARSRARKQAYTHELEIKVSRLEEENEKLRRLKEVEKILPSEPPP 248
>At2g41070.1 68415.m05071 basic leucine zipper transcription factor
(BZIP12) nearly identical to basic leucine zipper
transcription factor [Arabidopsis thaliana] GI:21694632;
contains a bZIP transcription factor basic domain
signature (PDOC00036)
Length = 262
Score = 27.1 bits (57), Expect = 4.3
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +2
Query: 95 RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 238
R S + SR +A+T + +R+ EE+ ++ R E P PPP
Sbjct: 201 RESAARSRARKQAYTHELEIKVSRLEEENEKLRRLKEVEKILPSEPPP 248
>At4g29790.1 68417.m04242 expressed protein
Length = 1211
Score = 25.8 bits (54), Expect = 9.9
Identities = 14/36 (38%), Positives = 19/36 (52%), Gaps = 5/36 (13%)
Frame = +2
Query: 194 RASRAEHPEPE-----VPPPGLEALAHHRGGRLNQH 286
R+ R E E E +P G+ + AHH GRL+ H
Sbjct: 789 RSDRLEFDELEDDVSVIPLKGVNSSAHHVNGRLSDH 824
>At3g62240.1 68416.m06992 zinc finger (C2H2 type) family protein
contains Pfam PF00096: Zinc finger, C2H2 type
Length = 812
Score = 25.8 bits (54), Expect = 9.9
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = +1
Query: 28 KQTILDKCNDTIKWLDSNQLADKEEYEHKQKE 123
K+T+ D DT++ L S+Q +EE E K+
Sbjct: 691 KETLADNFMDTVRRLQSSQNPQEEEEEAISKD 722
>At3g60000.1 68416.m06699 hypothetical protein contains Pfam
profile: PF04484 family of unknown function (DUF566)
Length = 451
Score = 25.8 bits (54), Expect = 9.9
Identities = 15/48 (31%), Positives = 22/48 (45%)
Frame = +2
Query: 95 RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 238
R S + +R FT+ R+ ++ P + R E P P VPPP
Sbjct: 88 RSSETAARSLDSPFTLSQSRKSSKPSHLKPLNENSHRLETPTPMVPPP 135
>At3g16565.1 68416.m02118 alanyl-tRNA synthetase-related low
similarity to SP|Q56273 Alanyl-tRNA synthetase (EC
6.1.1.7) (Alanine--tRNA ligase) (AlaRS) {Thiobacillus
ferrooxidans}
Length = 271
Score = 25.8 bits (54), Expect = 9.9
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +1
Query: 94 KEEYEHKQKELEGIYNPIITK 156
+EE++ KQKELE N +I+K
Sbjct: 172 QEEFQVKQKELEAEANELISK 192
>At2g37870.1 68415.m04649 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein contains Pfam
protease inhibitor/seed storage/LTP family domain
PF00234
Length = 115
Score = 25.8 bits (54), Expect = 9.9
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = -2
Query: 142 DCKCLPILSACAHTPPCRPAGWNP 71
+C +PI A A PC PA NP
Sbjct: 26 ECGRMPINQAAASLSPCLPATKNP 49
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,298,465
Number of Sequences: 28952
Number of extensions: 130860
Number of successful extensions: 545
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 529
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 545
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 537681456
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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