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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30010
         (756 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1F12.02c |p23fy||translationally controlled tumor protein ho...    76   5e-15
SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces pom...    29   0.54 
SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ...    27   2.2  
SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|...    27   3.8  
SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||...    27   3.8  
SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomy...    27   3.8  
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc...    26   6.7  
SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi...    25   8.8  
SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyce...    25   8.8  
SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces...    25   8.8  

>SPAC1F12.02c |p23fy||translationally controlled tumor protein
           homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 168

 Score = 76.2 bits (179), Expect = 5e-15
 Identities = 39/84 (46%), Positives = 55/84 (65%)
 Frame = +1

Query: 259 DIVLNHRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILGR 438
           ++V + RL  T +F DKKSY  Y+K YMK + A+L+E  P++V VF+ N    +K IL  
Sbjct: 67  NLVYSFRLSPT-SF-DKKSYMSYIKGYMKAIKARLQESNPERVPVFEKNAIGFVKKILAN 124

Query: 439 FKELQFFTGESMDCDGMVAMMEYK 510
           FK+  F+ GESMD D MV +M Y+
Sbjct: 125 FKDYDFYIGESMDPDAMVVLMNYR 148



 Score = 66.9 bits (156), Expect = 3e-12
 Identities = 31/65 (47%), Positives = 46/65 (70%), Gaps = 1/65 (1%)
 Frame = +2

Query: 62  MKIYKDIITGDEMFSDTYKMKLVDEVIYEVTGRLVTRAQ-GDIQIEGFNPSAEEADEGTD 238
           M +YKD+I+GDE+ SD Y +K VD+++YE   ++VT  Q GD+ I G NPSAE+A+E  +
Sbjct: 1   MLLYKDVISGDELVSDAYDLKEVDDIVYEADCQMVTVKQGGDVDI-GANPSAEDAEENAE 59

Query: 239 SAVES 253
              E+
Sbjct: 60  EGTET 64


>SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 355

 Score = 29.5 bits (63), Expect = 0.54
 Identities = 17/49 (34%), Positives = 26/49 (53%)
 Frame = +1

Query: 256 VDIVLNHRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKT 402
           +D V  ++L+E   F ++K  T+YLK   + L  K +   PD VE   T
Sbjct: 89  IDEVSLNKLIEKVGFHNRK--TIYLKQMARILSEKFQGDIPDTVEDLMT 135


>SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor
           Ste6|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 911

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 15/33 (45%), Positives = 20/33 (60%)
 Frame = -3

Query: 616 ASLELLSYFLSNI*NFSSSRPCLKNIMIGICVP 518
           AS ELL+       NFS+ R CL+N ++  CVP
Sbjct: 781 ASFELLNNLTEARKNFSNYRDCLENCVLP-CVP 812


>SPBC16D10.04c |dna2||DNA replication endonuclease-helicase
           Dna2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1398

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 13/38 (34%), Positives = 18/38 (47%)
 Frame = -1

Query: 531 VFAYHQSLIFHHGNHAITIHGLPSKELKFLKPAEDVFH 418
           V   H+  IFH GNH         ++LK +  A D+ H
Sbjct: 363 VLEVHEDFIFHEGNH--------FRQLKLILEANDILH 392


>SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1367

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 13/23 (56%), Positives = 16/23 (69%)
 Frame = -1

Query: 99   ISSPVIMSL*IFILMDWRRLKII 31
            ISSP I  + IFILM+  RL +I
Sbjct: 1220 ISSPTIFVINIFILMNQERLNLI 1242


>SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 654

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 13/37 (35%), Positives = 21/37 (56%)
 Frame = -3

Query: 121 HFVSVREHLITSDNVLIDLHFDGLEAIKNNKNRKNGF 11
           +F  + + L T +  LI   +  +E+IK  KNR +GF
Sbjct: 503 YFSHISDSLTTEELELILRQYGEIESIKYLKNRSSGF 539


>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1016

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 16/46 (34%), Positives = 21/46 (45%)
 Frame = +2

Query: 200 FNPSAEEADEGTDSAVESELT*S*TTG*SKHTPSVTRNPTHCTSKT 337
           +NPS    D G    V+S  T S  T     T S+T  PT  ++ T
Sbjct: 826 YNPSQYGCDNGALGPVQSSSTTSSITPTPTTTSSITPTPTTTSTTT 871


>SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 749

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 13/41 (31%), Positives = 20/41 (48%)
 Frame = -3

Query: 586 SNI*NFSSSRPCLKNIMIGICVPSKSYIPSWQPCHHNPWTP 464
           S + NF+S  P     +    +PS++  PS QP +    TP
Sbjct: 687 SGLSNFNSVTPSSSASLYPPLIPSEARTPSVQPANRRVTTP 727


>SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 481

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 9/27 (33%), Positives = 17/27 (62%)
 Frame = -1

Query: 456 ELKFLKPAEDVFHYFVHVCFKYFNLVR 376
           +  FLKP   ++ YF+ +  +Y +L+R
Sbjct: 175 QFDFLKPNNALYPYFMRIVQQYTSLIR 201


>SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 844

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 13/57 (22%), Positives = 26/57 (45%)
 Frame = +1

Query: 349 LVAKLEEKAPDQVEVFKTNMNKVMKDILGRFKELQFFTGESMDCDGMVAMMEYKTLM 519
           ++ K   KAP +   F++  N   K  +  FK+  +      D D +V + ++K  +
Sbjct: 587 IIGKWYYKAPKEFATFESAKNLNGKSFVDNFKDRYYM--YKQDIDNVVGLKDFKVYL 641


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,087,364
Number of Sequences: 5004
Number of extensions: 63088
Number of successful extensions: 169
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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