BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS30010 (756 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F12.02c |p23fy||translationally controlled tumor protein ho... 76 5e-15 SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces pom... 29 0.54 SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 27 2.2 SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|... 27 3.8 SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||... 27 3.8 SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomy... 27 3.8 SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 26 6.7 SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi... 25 8.8 SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyce... 25 8.8 SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces... 25 8.8 >SPAC1F12.02c |p23fy||translationally controlled tumor protein homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 168 Score = 76.2 bits (179), Expect = 5e-15 Identities = 39/84 (46%), Positives = 55/84 (65%) Frame = +1 Query: 259 DIVLNHRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILGR 438 ++V + RL T +F DKKSY Y+K YMK + A+L+E P++V VF+ N +K IL Sbjct: 67 NLVYSFRLSPT-SF-DKKSYMSYIKGYMKAIKARLQESNPERVPVFEKNAIGFVKKILAN 124 Query: 439 FKELQFFTGESMDCDGMVAMMEYK 510 FK+ F+ GESMD D MV +M Y+ Sbjct: 125 FKDYDFYIGESMDPDAMVVLMNYR 148 Score = 66.9 bits (156), Expect = 3e-12 Identities = 31/65 (47%), Positives = 46/65 (70%), Gaps = 1/65 (1%) Frame = +2 Query: 62 MKIYKDIITGDEMFSDTYKMKLVDEVIYEVTGRLVTRAQ-GDIQIEGFNPSAEEADEGTD 238 M +YKD+I+GDE+ SD Y +K VD+++YE ++VT Q GD+ I G NPSAE+A+E + Sbjct: 1 MLLYKDVISGDELVSDAYDLKEVDDIVYEADCQMVTVKQGGDVDI-GANPSAEDAEENAE 59 Query: 239 SAVES 253 E+ Sbjct: 60 EGTET 64 >SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces pombe|chr 1|||Manual Length = 355 Score = 29.5 bits (63), Expect = 0.54 Identities = 17/49 (34%), Positives = 26/49 (53%) Frame = +1 Query: 256 VDIVLNHRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKT 402 +D V ++L+E F ++K T+YLK + L K + PD VE T Sbjct: 89 IDEVSLNKLIEKVGFHNRK--TIYLKQMARILSEKFQGDIPDTVEDLMT 135 >SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor Ste6|Schizosaccharomyces pombe|chr 3|||Manual Length = 911 Score = 27.5 bits (58), Expect = 2.2 Identities = 15/33 (45%), Positives = 20/33 (60%) Frame = -3 Query: 616 ASLELLSYFLSNI*NFSSSRPCLKNIMIGICVP 518 AS ELL+ NFS+ R CL+N ++ CVP Sbjct: 781 ASFELLNNLTEARKNFSNYRDCLENCVLP-CVP 812 >SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|Schizosaccharomyces pombe|chr 2|||Manual Length = 1398 Score = 26.6 bits (56), Expect = 3.8 Identities = 13/38 (34%), Positives = 18/38 (47%) Frame = -1 Query: 531 VFAYHQSLIFHHGNHAITIHGLPSKELKFLKPAEDVFH 418 V H+ IFH GNH ++LK + A D+ H Sbjct: 363 VLEVHEDFIFHEGNH--------FRQLKLILEANDILH 392 >SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1367 Score = 26.6 bits (56), Expect = 3.8 Identities = 13/23 (56%), Positives = 16/23 (69%) Frame = -1 Query: 99 ISSPVIMSL*IFILMDWRRLKII 31 ISSP I + IFILM+ RL +I Sbjct: 1220 ISSPTIFVINIFILMNQERLNLI 1242 >SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomyces pombe|chr 3|||Manual Length = 654 Score = 26.6 bits (56), Expect = 3.8 Identities = 13/37 (35%), Positives = 21/37 (56%) Frame = -3 Query: 121 HFVSVREHLITSDNVLIDLHFDGLEAIKNNKNRKNGF 11 +F + + L T + LI + +E+IK KNR +GF Sbjct: 503 YFSHISDSLTTEELELILRQYGEIESIKYLKNRSSGF 539 >SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1016 Score = 25.8 bits (54), Expect = 6.7 Identities = 16/46 (34%), Positives = 21/46 (45%) Frame = +2 Query: 200 FNPSAEEADEGTDSAVESELT*S*TTG*SKHTPSVTRNPTHCTSKT 337 +NPS D G V+S T S T T S+T PT ++ T Sbjct: 826 YNPSQYGCDNGALGPVQSSSTTSSITPTPTTTSSITPTPTTTSTTT 871 >SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schizosaccharomyces pombe|chr 2|||Manual Length = 749 Score = 25.4 bits (53), Expect = 8.8 Identities = 13/41 (31%), Positives = 20/41 (48%) Frame = -3 Query: 586 SNI*NFSSSRPCLKNIMIGICVPSKSYIPSWQPCHHNPWTP 464 S + NF+S P + +PS++ PS QP + TP Sbjct: 687 SGLSNFNSVTPSSSASLYPPLIPSEARTPSVQPANRRVTTP 727 >SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyces pombe|chr 1|||Manual Length = 481 Score = 25.4 bits (53), Expect = 8.8 Identities = 9/27 (33%), Positives = 17/27 (62%) Frame = -1 Query: 456 ELKFLKPAEDVFHYFVHVCFKYFNLVR 376 + FLKP ++ YF+ + +Y +L+R Sbjct: 175 QFDFLKPNNALYPYFMRIVQQYTSLIR 201 >SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 844 Score = 25.4 bits (53), Expect = 8.8 Identities = 13/57 (22%), Positives = 26/57 (45%) Frame = +1 Query: 349 LVAKLEEKAPDQVEVFKTNMNKVMKDILGRFKELQFFTGESMDCDGMVAMMEYKTLM 519 ++ K KAP + F++ N K + FK+ + D D +V + ++K + Sbjct: 587 IIGKWYYKAPKEFATFESAKNLNGKSFVDNFKDRYYM--YKQDIDNVVGLKDFKVYL 641 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,087,364 Number of Sequences: 5004 Number of extensions: 63088 Number of successful extensions: 169 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 157 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 169 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 361294920 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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