Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS01034
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 160 2e-40
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 157 2e-39
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 101 8e-23
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 75 1e-14
SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|S... 30 0.31
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 28 1.2
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 1.6
SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 26 5.0
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.6
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 26 6.6
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.7
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 160 bits (389), Expect = 2e-40
Identities = 68/100 (68%), Positives = 83/100 (83%)
Frame = +1
Query: 220 FQHFLQRDRSWQHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 399
F F ++VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY
Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108
Query: 400 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTALG 519
T+GKE+VD V D+IR++AD C+GLQGFL+FHSFGGGT G
Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSG 148
Score = 105 bits (252), Expect = 7e-24
Identities = 45/63 (71%), Positives = 58/63 (92%)
Frame = +3
Query: 510 GSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVD 689
GSGF +LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVD
Sbjct: 146 GSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVD 205
Query: 690 NEA 698
NE+
Sbjct: 206 NES 208
Score = 96.7 bits (230), Expect = 3e-21
Identities = 42/63 (66%), Positives = 46/63 (73%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGS 256
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG G
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 257 TYP 265
P
Sbjct: 61 YVP 63
Score = 31.9 bits (69), Expect = 0.10
Identities = 13/14 (92%), Positives = 13/14 (92%)
Frame = +2
Query: 695 SIYDICRRNLDIER 736
S YDICRRNLDIER
Sbjct: 208 SCYDICRRNLDIER 221
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 157 bits (381), Expect = 2e-39
Identities = 65/100 (65%), Positives = 83/100 (83%)
Frame = +1
Query: 220 FQHFLQRDRSWQHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 399
F F + VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY
Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112
Query: 400 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTALG 519
T+GKE++D VL+RIR++AD C+GLQGFL+FHSFGGGT G
Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSG 152
Score = 108 bits (259), Expect = 1e-24
Identities = 46/63 (73%), Positives = 58/63 (92%)
Frame = +3
Query: 510 GSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVD 689
GSG +LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVD
Sbjct: 150 GSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVD 209
Query: 690 NEA 698
NEA
Sbjct: 210 NEA 212
Score = 90.6 bits (215), Expect = 2e-19
Identities = 46/68 (67%), Positives = 48/68 (70%), Gaps = 5/68 (7%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 241
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 242 TGAGSTYP 265
TG G P
Sbjct: 60 TGQGKFVP 67
Score = 31.1 bits (67), Expect = 0.18
Identities = 14/15 (93%), Positives = 14/15 (93%)
Frame = +2
Query: 701 YDICRRNLDIERQPT 745
YDICRRNLDIER PT
Sbjct: 214 YDICRRNLDIER-PT 227
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 101 bits (243), Expect = 8e-23
Identities = 45/89 (50%), Positives = 61/89 (68%)
Frame = +1
Query: 253 QHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVL 432
++VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VL
Sbjct: 58 KYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVL 117
Query: 433 DRIRKLADQCTGLQGFLIFHSFGGGTALG 519
D +R+ A+ C LQGF + HS GGGT G
Sbjct: 118 DVVRREAEACDALQGFQLTHSLGGGTGSG 146
Score = 61.7 bits (143), Expect = 1e-10
Identities = 27/63 (42%), Positives = 41/63 (65%)
Frame = +3
Query: 510 GSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVD 689
GSG +LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +D
Sbjct: 144 GSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCID 203
Query: 690 NEA 698
NEA
Sbjct: 204 NEA 206
Score = 38.7 bits (86), Expect = 9e-04
Identities = 20/63 (31%), Positives = 27/63 (42%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGS 256
MRE + + GQ G Q+G A W EHG+ G T + N +F+E G
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 257 TYP 265
P
Sbjct: 59 YVP 61
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 74.9 bits (176), Expect = 1e-14
Identities = 38/99 (38%), Positives = 60/99 (60%), Gaps = 2/99 (2%)
Frame = +1
Query: 229 FLQRDRSWQHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 402
F Q D + +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+
Sbjct: 52 FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109
Query: 403 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTALG 519
+ I + ++D I + AD L+GF + HS GGT G
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSG 148
Score = 50.4 bits (115), Expect = 3e-07
Identities = 30/82 (36%), Positives = 46/82 (56%), Gaps = 2/82 (2%)
Frame = +3
Query: 510 GSGFTSLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMV 686
GSG S L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D ++
Sbjct: 146 GSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVL 205
Query: 687 DNEA-SMTSAAVISTLSANLHQ 749
DN A + +A + T + HQ
Sbjct: 206 DNAALAHIAADRLHTQNPTFHQ 227
Score = 48.8 bits (111), Expect = 8e-07
Identities = 24/55 (43%), Positives = 33/55 (60%)
Frame = +2
Query: 80 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSET 244
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQS 55
>SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 30.3 bits (65), Expect = 0.31
Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = +3
Query: 555 YGKKSKLEFAIYPAPQVSTAVVE-PYNSILTTHTTLEHSDCAFMVDNEASM 704
Y + K+E YP ++ V + PYN +L ++LE ++DN S+
Sbjct: 110 YNSRWKIEIPSYPVREIPYVVADTPYNKLLCPLSSLEWVSKLILMDNANSI 160
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 28.3 bits (60), Expect = 1.2
Identities = 14/46 (30%), Positives = 26/46 (56%)
Frame = -1
Query: 564 SCRSQRRDAPSIRK*TQSRTSTEGVEDQESLQTGTLVSELADSVQN 427
S + SIR ++SR+S+ G + Q S+Q+GT ++D ++
Sbjct: 98 SSQKSSNSRKSIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 27.9 bits (59), Expect = 1.6
Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = -1
Query: 312 ADLINNSRFKIDEDS--TGYVLPAPVSLKKVLKESSP 208
AD+IN SR+ ID S T + P+SL KE P
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491
>SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 399
Score = 26.2 bits (55), Expect = 5.0
Identities = 14/58 (24%), Positives = 25/58 (43%), Gaps = 2/58 (3%)
Frame = -1
Query: 345 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLPAPVSLKKVLKESSPPPMVLSVGIW 178
F + + S +++N+ + KID GY S K + E + P + G+W
Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.6
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 175 WPDAHRQDHRGWRRFFQHFLQR 240
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 25.8 bits (54), Expect = 6.6
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = -1
Query: 279 DEDSTGYVLPAPVSLKKVLKESSPPPMVLSVGIWPSG 169
++D T Y+ P P+ + L +++PP +V+ +G P G
Sbjct: 834 EKDLTKYLGP-PIYTSQRLYDTTPPGVVMGLGWTPMG 869
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.7
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 66 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 188
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,122,852
Number of Sequences: 5004
Number of extensions: 66265
Number of successful extensions: 214
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 211
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -