Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS01011
(761 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 77 2e-15
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 50 5e-07
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 43 4e-05
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 37 0.004
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 36 0.005
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 33 0.034
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 28 1.7
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 27 2.2
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 27 3.9
SPAC3C7.05c |mug191||alpha-1,6-mannanase |Schizosaccharomyces po... 26 5.1
SPAC6F6.11c |||pyridoxine-pyridoxal-pyridoxamine kinase |Schizos... 26 6.7
SPAC23D3.03c |||GTPase activating protein |Schizosaccharomyces p... 25 8.9
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 25 8.9
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 25 8.9
SPAC23H4.14 |vam6|vps39|guanyl-nucleotide exchange factor Vma6|S... 25 8.9
SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium tra... 25 8.9
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 77.4 bits (182), Expect = 2e-15
Identities = 37/85 (43%), Positives = 57/85 (67%), Gaps = 1/85 (1%)
Frame = +1
Query: 256 ALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKYMRAQVGPSSK 435
+L +VDCTE G C ++S+ GYPTL +F+ G+ S+Y+GPR+ + +VKYMR Q+ P+ K
Sbjct: 74 SLVEVDCTEEG-DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPTVK 132
Query: 436 ELLTVADFEAFTSK-DEVVVVGFFE 507
++ E F K D++ VV FF+
Sbjct: 133 P-ISKDTLENFVEKADDLAVVAFFK 156
Score = 50.8 bits (116), Expect = 2e-07
Identities = 23/57 (40%), Positives = 32/57 (56%)
Frame = +2
Query: 74 YLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTD 244
+ C +AE V + + +++ +V FYAPWCGHCK L PEY AA L+ D
Sbjct: 17 FFCASAE--VPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADELEKD 71
Score = 47.6 bits (108), Expect = 2e-06
Identities = 20/43 (46%), Positives = 27/43 (62%), Gaps = 1/43 (2%)
Frame = +2
Query: 89 AEEDVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEY 214
++ED++ L +F V+ + LV FYAPWCGHCK L P Y
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTY 395
Score = 27.1 bits (57), Expect = 2.9
Identities = 19/64 (29%), Positives = 30/64 (46%), Gaps = 4/64 (6%)
Frame = +1
Query: 259 LAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSS--EYNGPRESNGIVKYM--RAQVGP 426
+AK+D TE S S+SG+PT+ F+ + + Y G R + ++ A P
Sbjct: 410 VAKIDATENDISV----SISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDKHASFEP 465
Query: 427 SSKE 438
KE
Sbjct: 466 IKKE 469
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 49.6 bits (113), Expect = 5e-07
Identities = 24/75 (32%), Positives = 38/75 (50%)
Frame = +2
Query: 11 KAPAKFEMFGSLKFVLLLGIIYLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGH 190
+ P F +F + F L+ G+ + ++L +F + +LV+FYAPWCG+
Sbjct: 4 RIPTLFTLFLAC-FSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGY 62
Query: 191 CKRLKPEYAVAAGLL 235
CK+L P Y A L
Sbjct: 63 CKKLVPTYQKLASNL 77
Score = 39.5 bits (88), Expect = 5e-04
Identities = 22/66 (33%), Positives = 37/66 (56%), Gaps = 6/66 (9%)
Frame = +1
Query: 268 VDC-TEGGKSTCEQFSVSGYPTLKIF---RKGE--LSSEYNGPRESNGIVKYMRAQVGPS 429
VDC + ++ C Q+ V G+PT+K+ KG S++YNG R + K++ + PS
Sbjct: 86 VDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDSI-PS 144
Query: 430 SKELLT 447
++LT
Sbjct: 145 KVKILT 150
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 43.2 bits (97), Expect = 4e-05
Identities = 19/50 (38%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = +2
Query: 98 DVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTD 244
+V++L +F V+ LV FYA WCG+CKRL P Y + K +
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKNE 190
Score = 39.5 bits (88), Expect = 5e-04
Identities = 15/28 (53%), Positives = 17/28 (60%)
Frame = +2
Query: 155 ALVMFYAPWCGHCKRLKPEYAVAAGLLK 238
AL+ FYA WCGHCK L P Y L +
Sbjct: 42 ALIEFYATWCGHCKSLAPVYEELGALFE 69
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 36.7 bits (81), Expect = 0.004
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = +2
Query: 119 SDFSAVLSQHDTALVMFYAPWCGHCKRLKPEY 214
S+F +++ Q +V F+A WCG CK + P++
Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF 40
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 36.3 bits (80), Expect = 0.005
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = +2
Query: 122 DFSAVLSQHDTALVMFYAPWCGHCKRLKP 208
D++ +S +V FYA WCG CK LKP
Sbjct: 27 DYNTRISADKVTVVDFYADWCGPCKYLKP 55
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 33.5 bits (73), Expect = 0.034
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +2
Query: 110 LTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKP 208
LTD+D + +S+ T + +Y P CG CKRL P
Sbjct: 31 LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGP 62
Score = 28.3 bits (60), Expect = 1.3
Identities = 15/49 (30%), Positives = 25/49 (51%)
Frame = +1
Query: 259 LAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKY 405
+A ++C K C+Q+S+ +PT +F K E EY G +V +
Sbjct: 333 VAHINCAVS-KRACKQYSIQYFPTF-LFFKEEAFVEYVGLPNEGDLVSF 379
>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2052
Score = 27.9 bits (59), Expect = 1.7
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +2
Query: 566 TFAHSSANEVLEKTGYKNNVVLYRPKRLQNKFEDSSVAF 682
T + S +NE+ EKT K + L + K++ +F D +F
Sbjct: 1265 TSSTSDSNEI-EKTQEKKRLALEKQKKIMQQFRDQQASF 1302
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 27.5 bits (58), Expect = 2.2
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +2
Query: 170 YAPWCGHCKRLKPEYAVAA 226
YA WCG CK + P ++ A
Sbjct: 27 YADWCGPCKAISPLFSQLA 45
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 26.6 bits (56), Expect = 3.9
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +3
Query: 102 FSILQIPTFRLFYLNMIQPWSCF 170
F LQ+ FR + N+++PW CF
Sbjct: 208 FYCLQLQMFRKMH-NIVRPWDCF 229
>SPAC3C7.05c |mug191||alpha-1,6-mannanase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 442
Score = 26.2 bits (55), Expect = 5.1
Identities = 19/67 (28%), Positives = 30/67 (44%), Gaps = 1/67 (1%)
Frame = -1
Query: 527 LFQVRFLSKNPTTTTS-SLEVKASKSATVRSSLELGPTWARMYLTMPLDSLGPLYSEESS 351
L + RF TT T+ SL + + +E PT+ LD+ GPLY + +
Sbjct: 229 LDKARFTYNTGTTMTAMSLLMGLGEFTKGLQDIEKLPTYLEDMARGALDTNGPLYDQSCN 288
Query: 350 PFLKIFS 330
K++S
Sbjct: 289 GSFKVWS 295
>SPAC6F6.11c |||pyridoxine-pyridoxal-pyridoxamine kinase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 309
Score = 25.8 bits (54), Expect = 6.7
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +2
Query: 545 DKLREEVTFAHSSANEVLEKT 607
DKL++ V A SS +EV++KT
Sbjct: 248 DKLKKSVEMALSSVHEVIQKT 268
>SPAC23D3.03c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 472
Score = 25.4 bits (53), Expect = 8.9
Identities = 29/106 (27%), Positives = 48/106 (45%), Gaps = 1/106 (0%)
Frame = +2
Query: 107 DLTDSDF-SAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPPGR*LK*TVRK 283
+L D F AV +Q ++L FY + K+ +PE A L+K + P + +RK
Sbjct: 338 NLLDKPFLQAVYTQDTSSLKSFYQTFLDTLKKNEPELATHL-LIKLELVPDDFVYPLLRK 396
Query: 284 VARVLVNNSLCPDILH*KSSEKENFLPSTMDQGSLMALSSTCVPKL 421
+ +V+ + IL E+++F LMA+ PKL
Sbjct: 397 LFIPMVSPEIASRILDCYVFEEDSFFIQL-----LMAVFKLLKPKL 437
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 25.4 bits (53), Expect = 8.9
Identities = 8/15 (53%), Positives = 11/15 (73%)
Frame = +2
Query: 158 LVMFYAPWCGHCKRL 202
L+ FYAPW CK++
Sbjct: 24 LLNFYAPWAAPCKQM 38
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 25.4 bits (53), Expect = 8.9
Identities = 10/31 (32%), Positives = 18/31 (58%)
Frame = -1
Query: 422 PTWARMYLTMPLDSLGPLYSEESSPFLKIFS 330
P ++Y+T+ D +G E +SP +K+ S
Sbjct: 1414 PQKEKLYITISADEVGKFILEATSPTVKVSS 1444
>SPAC23H4.14 |vam6|vps39|guanyl-nucleotide exchange factor
Vma6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 905
Score = 25.4 bits (53), Expect = 8.9
Identities = 13/51 (25%), Positives = 22/51 (43%)
Frame = +2
Query: 518 PEREFLKTADKLREEVTFAHSSANEVLEKTGYKNNVVLYRPKRLQNKFEDS 670
P+ + D+ E T + + LE YK +++ L NKF D+
Sbjct: 606 PQNSIVIFIDENSEASTISKGVVLKYLETISYKVSIIYLEKLLLDNKFNDT 656
>SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium
transporting Cta4 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1211
Score = 25.4 bits (53), Expect = 8.9
Identities = 17/50 (34%), Positives = 27/50 (54%)
Frame = +2
Query: 92 EEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKT 241
EE V+D+ + D +AVL L + +P+ CK P+ V A +L+T
Sbjct: 324 EEAVIDVDELDKNAVLFGGTRVLQVTQSPF---CKLKTPDNGVPAIVLRT 370
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,121,157
Number of Sequences: 5004
Number of extensions: 64869
Number of successful extensions: 220
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 365309308
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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