Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00965
(640 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 129 3e-31
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 129 4e-31
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 129 4e-31
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 81 2e-16
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 79 7e-16
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 47 2e-06
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 29 0.75
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 27 2.3
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 26 4.0
SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomy... 26 5.3
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 25 7.0
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 9.2
SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch... 25 9.2
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 9.2
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 25 9.2
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 129 bits (312), Expect = 3e-31
Identities = 62/84 (73%), Positives = 72/84 (85%)
Frame = +2
Query: 257 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 436
ADCAVLI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFE
Sbjct: 109 ADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFE 166
Query: 437 EIKKEVSSYIKKIGYNPAAVAFVP 508
EI KE S++IKK+G+NP V FVP
Sbjct: 167 EIVKETSNFIKKVGFNPKTVPFVP 190
Score = 99.1 bits (236), Expect = 5e-22
Identities = 44/46 (95%), Positives = 45/46 (97%)
Frame = +1
Query: 1 TTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 138
TTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVLDKLKAERE
Sbjct: 23 TTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERE 68
Score = 66.9 bits (156), Expect = 2e-12
Identities = 27/51 (52%), Positives = 36/51 (70%)
Frame = +1
Query: 484 PSCCRFRALSGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAI 636
P F +SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I
Sbjct: 183 PKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSI 233
Score = 66.1 bits (154), Expect = 4e-12
Identities = 28/32 (87%), Positives = 30/32 (93%)
Frame = +3
Query: 162 LWKFETSKYYVTIIDAPGHRDFIKNMITGTSR 257
LWKFET KY VT+IDAPGHRDFIKNMITGTS+
Sbjct: 77 LWKFETPKYNVTVIDAPGHRDFIKNMITGTSQ 108
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 129 bits (311), Expect = 4e-31
Identities = 61/84 (72%), Positives = 72/84 (85%)
Frame = +2
Query: 257 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 436
ADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFE
Sbjct: 109 ADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFE 166
Query: 437 EIKKEVSSYIKKIGYNPAAVAFVP 508
EI KE S++IKK+G+NP V FVP
Sbjct: 167 EIVKETSNFIKKVGFNPKTVPFVP 190
Score = 99.1 bits (236), Expect = 5e-22
Identities = 44/46 (95%), Positives = 45/46 (97%)
Frame = +1
Query: 1 TTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 138
TTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVLDKLKAERE
Sbjct: 23 TTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERE 68
Score = 66.9 bits (156), Expect = 2e-12
Identities = 27/51 (52%), Positives = 36/51 (70%)
Frame = +1
Query: 484 PSCCRFRALSGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAI 636
P F +SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I
Sbjct: 183 PKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSI 233
Score = 66.1 bits (154), Expect = 4e-12
Identities = 28/32 (87%), Positives = 30/32 (93%)
Frame = +3
Query: 162 LWKFETSKYYVTIIDAPGHRDFIKNMITGTSR 257
LWKFET KY VT+IDAPGHRDFIKNMITGTS+
Sbjct: 77 LWKFETPKYNVTVIDAPGHRDFIKNMITGTSQ 108
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 129 bits (311), Expect = 4e-31
Identities = 61/84 (72%), Positives = 72/84 (85%)
Frame = +2
Query: 257 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 436
ADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFE
Sbjct: 109 ADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFE 166
Query: 437 EIKKEVSSYIKKIGYNPAAVAFVP 508
EI KE S++IKK+G+NP V FVP
Sbjct: 167 EIVKETSNFIKKVGFNPKTVPFVP 190
Score = 99.1 bits (236), Expect = 5e-22
Identities = 44/46 (95%), Positives = 45/46 (97%)
Frame = +1
Query: 1 TTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 138
TTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVLDKLKAERE
Sbjct: 23 TTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERE 68
Score = 66.9 bits (156), Expect = 2e-12
Identities = 27/51 (52%), Positives = 36/51 (70%)
Frame = +1
Query: 484 PSCCRFRALSGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAI 636
P F +SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I
Sbjct: 183 PKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSI 233
Score = 66.1 bits (154), Expect = 4e-12
Identities = 28/32 (87%), Positives = 30/32 (93%)
Frame = +3
Query: 162 LWKFETSKYYVTIIDAPGHRDFIKNMITGTSR 257
LWKFET KY VT+IDAPGHRDFIKNMITGTS+
Sbjct: 77 LWKFETPKYNVTVIDAPGHRDFIKNMITGTSQ 108
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 80.6 bits (190), Expect = 2e-16
Identities = 38/86 (44%), Positives = 58/86 (67%), Gaps = 2/86 (2%)
Frame = +2
Query: 257 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 436
AD VL+++A GEFEAG + GQTREHA+LA T G+ L+V +NKMD +SE R++
Sbjct: 340 ADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYK 399
Query: 437 EIKKEVSSYIKKI-GYNPAA-VAFVP 508
E ++S +++++ GYN V ++P
Sbjct: 400 ECVDKLSMFLRRVAGYNSKTDVKYMP 425
Score = 51.2 bits (117), Expect = 1e-07
Identities = 21/45 (46%), Positives = 32/45 (71%)
Frame = +1
Query: 7 GHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAEREE 141
G++++ G +DKRT+EK E+EA+E GK S+ +W LD ERE+
Sbjct: 256 GNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTSEEREK 300
Score = 35.5 bits (78), Expect = 0.007
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = +3
Query: 171 FETSKYYVTIIDAPGHRDFIKNMITGTSR 257
FET +++DAPGH+ ++ NMI G S+
Sbjct: 311 FETEHRRFSLLDAPGHKGYVTNMINGASQ 339
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 78.6 bits (185), Expect = 7e-16
Identities = 41/85 (48%), Positives = 55/85 (64%), Gaps = 1/85 (1%)
Frame = +2
Query: 257 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 436
AD AVL+V + FE G +NGQTREHA L LG+ +++V VNK+D +SE RF+
Sbjct: 279 ADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQ 336
Query: 437 EIKKEVSSY-IKKIGYNPAAVAFVP 508
EIK VS + IK +G+ + V FVP
Sbjct: 337 EIKNIVSDFLIKMVGFKTSNVHFVP 361
Score = 48.4 bits (110), Expect = 9e-07
Identities = 19/43 (44%), Positives = 29/43 (67%)
Frame = +1
Query: 7 GHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAER 135
G ++++ G I+ R+++K EA GKGSF YAW+LD + ER
Sbjct: 195 GRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTEEER 237
Score = 37.5 bits (83), Expect = 0.002
Identities = 17/28 (60%), Positives = 18/28 (64%)
Frame = +3
Query: 171 FETSKYYVTIIDAPGHRDFIKNMITGTS 254
FE+ K I DAPGHRDFI MI G S
Sbjct: 250 FESDKKIYEIGDAPGHRDFISGMIAGAS 277
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 47.2 bits (107), Expect = 2e-06
Identities = 27/68 (39%), Positives = 41/68 (60%)
Frame = +2
Query: 260 DCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEE 439
D A+++V+A G+ QTREH LLA +GVKQ++V +NK+D EP E E
Sbjct: 141 DGAIIVVSATDGQMP-------QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEME 193
Query: 440 IKKEVSSY 463
+++ +S Y
Sbjct: 194 MRELLSEY 201
Score = 38.7 bits (86), Expect = 7e-04
Identities = 14/31 (45%), Positives = 22/31 (70%)
Frame = +3
Query: 168 KFETSKYYVTIIDAPGHRDFIKNMITGTSRL 260
++ET+ + +D PGH D+IKNMITG + +
Sbjct: 110 EYETANRHYAHVDCPGHADYIKNMITGAATM 140
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 28.7 bits (61), Expect = 0.75
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = +2
Query: 482 NPAAVAFVPFLDGTETTCWSLQPKCLGSRDGRWSVKKAKLTENASLK 622
+PA + V LD + W++QP C + DG A L++NA LK
Sbjct: 139 SPAQITDVLQLDKLKDRLWNVQPTCALTGDGLLE-GLAWLSQNAKLK 184
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 27.1 bits (57), Expect = 2.3
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +3
Query: 186 YYVTIIDAPGHRDFIKNMITGTSRL 260
Y + +ID+PGH DF + ++ SRL
Sbjct: 94 YLINLIDSPGHVDF-SSEVSSASRL 117
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 26.2 bits (55), Expect = 4.0
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +3
Query: 171 FETSKYYVTIIDAPGHRDF 227
FE S Y + IID PGH DF
Sbjct: 141 FEKS-YNINIIDTPGHIDF 158
>SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 384
Score = 25.8 bits (54), Expect = 5.3
Identities = 18/60 (30%), Positives = 28/60 (46%)
Frame = -3
Query: 209 SINDGNIVLASFELPESNIDCDTSSRSAFSLSNTQAYLKDPLPISWASFSNFSMVRLSIP 30
S++ + L S+ P S T+ A ++YL+ PL S S S + +R SIP
Sbjct: 305 SVDSASTALNSYSTPNSVSRKLTNLTPAARRLVARSYLRSPLHGSSPSASRHTALRTSIP 364
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 25.4 bits (53), Expect = 7.0
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +3
Query: 417 TVSPDLRKSRRKYPHTSRRLATTQLLSLSC 506
+ +PD+ ++PHT +R +T + L + C
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRC 174
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 25.0 bits (52), Expect = 9.2
Identities = 12/47 (25%), Positives = 22/47 (46%)
Frame = +2
Query: 326 QTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 466
QT EH + +K +I+ NK+D +E ++ I K + +
Sbjct: 149 QTSEHLAAIEIMQLKHIIILQNKVDLIRESAAEEHYQSILKFIKGTV 195
>SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 791
Score = 25.0 bits (52), Expect = 9.2
Identities = 14/44 (31%), Positives = 25/44 (56%)
Frame = -1
Query: 313 DTSFEFTGTGSYDEHSAISLEVPVIMFLMKSLCPGASMMVT*YL 182
D+ +E T Y E+S + L + I+ + SL +SMM++ +L
Sbjct: 399 DSKWELV-TQKYQEYSPVMLPIAFIINIALSLGAFSSMMISFFL 441
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 9.2
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -1
Query: 76 PGPPSRTSRWYVCQYHHIC 20
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 25.0 bits (52), Expect = 9.2
Identities = 8/22 (36%), Positives = 13/22 (59%)
Frame = +3
Query: 162 LWKFETSKYYVTIIDAPGHRDF 227
++ + Y + +ID PGH DF
Sbjct: 116 IYYYHGQSYLLNLIDTPGHVDF 137
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,772,879
Number of Sequences: 5004
Number of extensions: 58508
Number of successful extensions: 244
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 240
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -