BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00964
(698 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC56F8.10 |met9|met5|methylenetetrahydrofolate reductase Met9|... 31 0.16
SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po... 28 1.5
SPCC320.05 |||sulphate transporter |Schizosaccharomyces pombe|ch... 27 2.0
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 27 3.4
SPAC1071.05 |||S-adenosylmethionine-dependent methyltransferase ... 26 4.5
SPBC16D10.02 |trm11||tRNA |Schizosaccharomyces pombe|chr 2|||Manual 26 4.5
SPAC4F10.06 |||BUD22 family protein|Schizosaccharomyces pombe|ch... 25 7.9
SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog... 25 7.9
>SPAC56F8.10 |met9|met5|methylenetetrahydrofolate reductase
Met9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 603
Score = 31.1 bits (67), Expect = 0.16
Identities = 12/52 (23%), Positives = 27/52 (51%)
Frame = -3
Query: 270 LLSPSRVVSWNDNRYAWNVWWNNSCGFTSLSEYLGIQEFRGKYFCKILNSDF 115
++ + ++W D Y+ + W N+ S+S L + + + C I+++DF
Sbjct: 523 IVESTSFLAWKDEAYSLGMEWANAYSPDSISRKLLVSMMKEWFLCVIVDNDF 574
>SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1014
Score = 27.9 bits (59), Expect = 1.5
Identities = 13/46 (28%), Positives = 24/46 (52%)
Frame = +3
Query: 165 FPSIHSSS*NRSCYSTRRSTHTYYHSTRLHATDLEEEHRLAYWRED 302
FPS+ ++ R CY R +++ +LH LEE++ + + D
Sbjct: 697 FPSLRYNTNRRFCYVQMRKPDEAHNALQLHKKLLEEKYPIQVFISD 742
>SPCC320.05 |||sulphate transporter |Schizosaccharomyces pombe|chr
3|||Manual
Length = 667
Score = 27.5 bits (58), Expect = 2.0
Identities = 15/50 (30%), Positives = 23/50 (46%)
Frame = +2
Query: 35 PLHE*T*TLSCSTIVTLWH*CTDVIPEKSELRILQKYFPLNSWIPKYSLK 184
PLHE S + + W + S +R + Y P+ W+P YSL+
Sbjct: 26 PLHEIPSYQSLARRSSTWKRANIPQQKPSLVRRINYYIPVLHWLPNYSLR 75
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1207
Score = 26.6 bits (56), Expect = 3.4
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
Frame = -3
Query: 300 LLASTQGGVLLLSPSRVVS-WNDNRYAWNVWWNNSCGFTSLSEYLGIQEFRGKYFCKILN 124
L +TQ + L ++VS +N NR + VW N++ L GK++ I+
Sbjct: 473 LSTATQVHLFDLQQKKIVSSFNANRVKYVVWSNDNSQAALL----------GKHYVYIVK 522
Query: 123 SDFSGITSVHQCHRV 79
+ ITS+H+ R+
Sbjct: 523 KNLELITSIHETIRI 537
>SPAC1071.05 |||S-adenosylmethionine-dependent methyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 339
Score = 26.2 bits (55), Expect = 4.5
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = -3
Query: 294 ASTQGGVLLLSPSRVVSWNDNRYAWNVWWNNSCGFTSLSEYLGIQEFRG-KYFCKILNSD 118
+S + G + +SPS + ++D+ N++ CG S L IQ G YF +L S+
Sbjct: 187 SSEKHGTIDVSPSLLQEFSDDLARTNIYCEFLCGCWSEEMQLLIQRTYGDHYFSLVLASE 246
>SPBC16D10.02 |trm11||tRNA |Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 26.2 bits (55), Expect = 4.5
Identities = 14/43 (32%), Positives = 24/43 (55%)
Frame = +2
Query: 398 VLLYAPASDSPLQLRAFVQLIKKS*KIQQLEGANSGGYFPKLD 526
+LL A D P +RA + I K + E +++G ++PKL+
Sbjct: 310 LLLDAIVCDPPYGVRAGAKKIAKCSQRPPKESSSTGNHYPKLE 352
>SPAC4F10.06 |||BUD22 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 388
Score = 25.4 bits (53), Expect = 7.9
Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +1
Query: 70 NYCYSVALMHRRDTRKVRVKNFAEVFPSKFL-DSQVFTQAR 189
+YCY ++ +D RK+ +N FP+ D+Q T AR
Sbjct: 85 DYCYYKKILKNKDFRKLLNENLHVDFPADLTEDAQRNTVAR 125
>SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 982
Score = 25.4 bits (53), Expect = 7.9
Identities = 15/50 (30%), Positives = 25/50 (50%), Gaps = 4/50 (8%)
Frame = +2
Query: 515 PKLDSLTSSRG--WPPRQSGMQW--QDLNRAAEGLFVTIDEMERWRRNVE 652
PK+ + S++ SG Q Q++ EG+ + D M +WR NV+
Sbjct: 893 PKIIEMASNKAAELEAEDSGAQGDTQEVKSKKEGMAIVRDIMRQWRSNVK 942
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,059,368
Number of Sequences: 5004
Number of extensions: 66417
Number of successful extensions: 170
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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